Abstract：
:We have previously used the antibody-mediated binding of bacteria to identify Ig-bearing cells and the natural binding of bacteria to identify several lymphocyte subpopulations. By using bacteria this identification can be carried out in conventional blood smears since bacteria are small and easily distinguished from all blood elements. To develop a standardized method for identification of lymphocyte subpopulations that may become useful in clinical laboratories, we investigated here several parameters that might affect the safety and accuracy of the test, and that might simplify the procedure. We found that: (1) the rosettes formed between bacteria and lymphocytes cannot be disrupted by vigorous handling; (2) the ability fo form rosettes is a stable property of the bacterial strains; (3) for optimal results the blood sample must not be stored for more than 4 hr at 25 degrees C and the entire procedure must be performed in medium supplemented with 6% bovine serum albumin; (4) a large excess of anti-Ig antibody is required for the optimal coating of bacteria to detect Ig-bearing cells; (5) both the antibody-coated bacteria and formaldehyde-fixed bacteria can be stored at 4 degrees C or at -20 degrees C for at least 6 months; (6) the buffy coat from the blood sample can be used instead of the whole blood to reduce the time required for reading the smears; and (7) some of the pathogenic bacteria can be killed by autoclaving without modifying their binding properties. A complete and simple method which uses the bacterial adherence for the identification of lymphocyte subpopulations in blood smears is presented.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Bratescu A,Mayer E,Teodorescu Msubject
Has Abstractpub_date
1980-09-01 00:00:00pages
547-58issue
3eissn
0009-9104issn
1365-2249journal_volume
41pub_type
杂志文章abstract：:Cellular sensitization to rabbit liver specific lipoprotein (RLSP) has been investigated using a lymphocyte transformation test in patients with chronic active hepatitis (CAH). A stimulation index greater than 2 was recorded in twenty out of twenty-five cases (eight of ten HBsAg positive and twelve of fiteen HBsAg neg...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1979-11-01 00:00:00
abstract：:Schizophrenic patients are reported to have immunological dysfunction, however, the immune response to surgery in schizophrenic patients remains unclear. We measured plasma interleukin-6 (IL-6), interleukin-8 (IL-8) and tumour necrosis factor-alpha (TNF-alpha) before, during and after colectomy, hemicolectomy and sigm...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2001.01581.x
更新日期：2001-07-01 00:00:00
abstract：:We describe the adoptive transfer of erosive arthritis to an immunodeficient host. Spleen cells from arthritic DBA/1 mice (H-2q), immunized 4-6 weeks previously with bovine type II collagen in adjuvant, were transferred intraperitoneally into SCID mice (H-2d). SCID recipient mice also received native or denatured type...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1992.tb06471.x
更新日期：1992-06-01 00:00:00
abstract：:The programmed death-1 (PD-1) receptor ligands, PD-L1 and PD-L2, are co-stimulatory molecules that contribute to the negative regulation of T lymphocyte activation. It is still unclear whether there is correlation between PD-L1 or PD-L2 and tumour-infiltrating dendritic cells (TIDCs) in cutaneous squamous cell carcino...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.12921
更新日期：2017-06-01 00:00:00
abstract：:The effector arm of the mucosal immune system comprises lymphocytes scattered at intraepithelial and lamina propria levels. Intraepithelial lymphocytes (IEL) are a large population of oligoclonal resting cells which exhibit phenotypic and functional characteristics of cytolytic T cells when activated. Several mechanis...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2000.01200.x
更新日期：2000-05-01 00:00:00
abstract：:Intestinal inflammation in inflammatory bowel disease (IBD) and experimental models of colitis is characterized by a dysregulated intestinal immune response with elevated levels of Th1 cytokines. The luminal flora has been implicated as a major factor contributing to the initiation and perpetuation of inflammation in ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2003.02288.x
更新日期：2003-11-01 00:00:00
abstract：:Circulating immune complexes have been investigated in jirds (Meriones unguiculatus) infected with the filarial nematode Brugia pahangi. Two-month-old male jirds were inoculated with seventy-five B. pahangi infective larvae into the left groin. At 8 months post-infection, sera of individual animals from a group of sev...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1980-05-01 00:00:00
abstract：:Two-stage stimulation of SK activity of normal human peripheral blood lymphocytes by soluble factors was demonstrated. The first stage was initiated by factors present in supernatant derived from normal B-LCL cultures. Only cell lines which could induce SK activity in culture in an MLR-type reaction had the capacity t...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1980-12-01 00:00:00
abstract：:The diagnostic value of the determination of the relative distribution of B (SmIg-positive) and T (E rosetting) cells in the blood and in pleural or ascitic effusions was evaluated in 10 patients with tuberculosis and in six with malignant diseases. A higher percentage of T and a lower percentage of B lymphocytes in s...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1981-07-01 00:00:00
abstract：:We have studied immunological, serological and clinical abnormalities in 264 HIV-positive and HIV-negative drug abusers. Ninety percent of the 264 drug addicts (mean age 26 +/- 0.8 years) were found to have HIV antibodies and there was a significant increase (P less than 0.01) in the frequency of HIV antibody positivi...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1988-05-01 00:00:00
abstract：:The precise factor responsible for the altered immunological reactivity and diminished inflammatory responses occurring in pregnancy is unknown. Recent observations implicate the pregnancy-associated alpha-2 glycoprotein (PAG). Using rosette assays for the detection of cells carrying Fc receptors and for the demonstra...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1981-11-01 00:00:00
abstract：:Ten out of 20 (50%) 17-week-old female NOD/WEHI mice developed an acute form of autoimmune diabetes when injected with two large doses of cyclophosphamide (CY), given at 14-day intervals. If these mice were treated under a prophylactic regimen with 2.5 mg/kg body weight per day of the novel immunosuppressant deoxysper...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1993.tb05888.x
更新日期：1993-02-01 00:00:00
abstract：:Psoriasis (Ps), psoriatic arthritis (PsA) and rheumatoid arthritis (RA) are common diseases dependent on environmental factors that activate the immune system in unknown ways. Mannan is a group of polysaccharides common in the environment; they are potentially pathogenic, because at least some of them induce Ps-, PsA-...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.13268
更新日期：2019-06-01 00:00:00
abstract：:Aspergillus fumigatus, a ubiquitous fungus, is implicated in the pathogenesis of a number of clinically different allergic diseases in man, including allergic bronchopulmonary aspergillosis. Peptide-based immunotherapy may offer an alternative treatment strategy for the management of allergic disease. The objective of...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.2002.01776.x
更新日期：2002-02-01 00:00:00
abstract：:Three B cell hybridomas were produced by the fusion of spleen cells from a 5 month old MRL/Mp-lpr/lpr mouse with the myeloma cell line, NS-1. By competitive inhibition, all three monoclonal antibodies (MoAb) were specific for poly(rA) and were inhibited to a lesser extent by dDNA, nDNA, poly(rI) and poly(rC). Moreover...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1985-07-01 00:00:00
abstract：:It has been shown that bronchoalveolar lavages (BALs) from patients with sarcoidosis and other interstitial lung diseases contain abnormally increased numbers of alveolar macrophages (AM) expressing antigens found on monocytes. The aim of this study was to compare the phenotype of AM from patients with sarcoidosis wit...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1991.tb05791.x
更新日期：1991-10-01 00:00:00
abstract：:Immunoenzymatic assays were developed for the measurement of antibodies against mycobacterial lipoarabinomannan (LAM), a cell-free proteic extract (CFX) of Mycobacterium leprae, and the 38-kD protein antigen of M. tuberculosis. Sera from 108 leprosy patients, belonging to all clinical-immunological forms of the spectr...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.1998.00452.x
更新日期：1998-01-01 00:00:00
abstract：:The functional integrity of the local immune system in vitamin A-deficient (A-) rats was investigated. Secretory IgA levels in the intestinal fluid of A- rats were significantly lower than in controls. This and the decrease in intensity of immunofluorescent staining for secretory component (SC) in the intestinal cells...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1980-04-01 00:00:00
abstract：:Both CC- and CXC-chemokines are known to be potent leucocyte activators and chemoattractants and play important roles in inflammatory responses. However, chemokine response to bacillus Calmette-Guérin (BCG) infection remains incompletely defined. In this study, we investigated human CC- [macrophage-derived chemokine (...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2006.03288.x
更新日期：2007-02-01 00:00:00
abstract：:In recent years galectin-3 has gained attention as a signalling molecule, mainly in inflammatory diseases. Data on galectin-3 expression in neonates, however, are limited, and expression of this lectin in cord blood has not yet been reported. The aim of this study was to determine galectin-3 levels in cord blood of te...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2011.04509.x
更新日期：2012-02-01 00:00:00
abstract：:Humoral antibodies to tumour associated membrane antigens of cultured human breast cancer cell lines were studied using the immune adherence (IA) test. Sera from 353 post-operative breast cancer patients and from twenty-five patients immunized by allogeneic breast cancer cells were tested against the MDA-MB-436 cell l...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1980-01-01 00:00:00
abstract：:The capacity of microbial products to inhibit allergic inflammation make them logical candidates for novel therapies in allergic diseases such as atopic dermatitis. To assess the effects of intradermal Mycobacterium vaccae derivative on allergen-specific immune responses in children with moderate to severe atopic derm...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2011.04371.x
更新日期：2011-06-01 00:00:00
abstract：:In a study of 75 alveolar cell co-cultures from 55 HIV-seropositive subjects, p24 antigen production was identified by ELISA in 11 out of 26 (42%) of unseparated cell cultures, 15 out of 39 (38%) purified alveolar macrophage cultures and eight out of 10 purified alveolar lymphocyte samples. Positivity in unseparated c...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1991-06-01 00:00:00
abstract：:Listeria monocytogenes (Listeria) are known to grow and proliferate in the liver while a splenectomy induces host resistance against a Listeria infection despite the fact that a splenectomy inhibits the Th1 response. Therefore, the mechanism by which a splenectomy helps to prevent the growth of Listeria still remains ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2005.02735.x
更新日期：2005-04-01 00:00:00
abstract：:Inadequate apoptosis may contribute to the synovial hyperplasia associated with rheumatoid arthritis (RA). The Fas-associated death domain protein (FADD)-like interleukin (IL)-1beta-converting enzyme (FLICE)-inhibitory protein (FLIP), which is an apoptotic inhibitor, has been implicated in the resistance to Fas-mediat...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2006.03070.x
更新日期：2006-06-01 00:00:00
abstract：:Psoriasis is an immune-mediated inflammatory skin disease that mainly affects the skin barrier. Treatment for psoriasis mainly includes conventional immunosuppressive drugs. However, long-term treatment with global immunosuppressive agents may cause a variety of side effects, including nephrotoxicity and infections. K...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.13363
更新日期：2019-12-01 00:00:00
abstract：:Natural killer (NK) cells mediate host defense against infections and are regulated by interleukin 2 (IL-2) and other factors. We studied NK cell function in burn patients using a 51Cr release assay with K562 target cells. We found that peripheral blood lymphocytes from burn patients had depressed NK activity (target ...
journal_title：Clinical and experimental immunology
pub_type： 临床试验,杂志文章,随机对照试验
doi：
更新日期：1988-01-01 00:00:00
abstract：:A 7 month old girl with severe combined immunodeficiency disease (SCID) received a single transfusion of peripheral blood leucocytes from her histocompatible grandfather in an attempt to achieve immunological reconstitution. There was rapid restoration of humoral and cellular immunity which has persisted undiminished ...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1986-06-01 00:00:00
abstract：:Neopterin is primarily synthesized and released by activated macrophages/monocytes upon stimulation with interferon-γ and is considered as a marker for macrophage activation. This study aimed to analyze the serum levels of neopterin in patients with dermatomyositis (DM) in association with clinical manifestations, lab...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.13404
更新日期：2020-03-01 00:00:00
abstract：:Increasing evidence has demonstrated that the immune system closely interacts with other physiological systems, whose communications are mediated by circulating cytokines and hormones. The aim of our study was to test whether the number and cytolytic activity of NK cells in a group of relatively healthy Italian nonage...
journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2249.1999.00855.x
更新日期：1999-04-01 00:00:00
