In vitro effect of carvedilol on professional phagocytes.


AIM:Superfluous reactive nitrogen and oxygen species generation is implicated in the damage of tissues at sites of inflammation where activated neutrophils and macrophages are involved. This study was conducted to investigate whether the beneficial effects of carvedilol involve modulation of respiratory burst, degranulation-myeloperoxidase release and inducible nitric oxide synthase (iNOS) expression. METHODS:Spectrophotometry and chemiluminescence were used to evaluate the effect of carvedilol on opsonized zymosan (0.5 mg/ml)- or N-formyl-methionyl-leucyl-phenyl-alanine (fMLP, 0.1 micromol/l)-stimulated superoxide generation and myeloperoxidase release in human neutrophils. Western blot analysis was used for iNOS expression and Griess reagent for nitric oxide production in RAW 264.7 macrophages (lipopolysaccharide (0.1 microg/ml) stimulated). RESULTS:Carvedilol (10 and 100 micromol/l) significantly decreased opsonized zymosan- and fMPL-stimulated superoxide generation and myeloperoxidase release. Carvedilol (100 micromol/l) enhanced the effect of wortmannin (50 nmol/l), a specific inhibitor of 1-phosphatidylinositol 3-kinase and decreased iNOS expression and nitric oxide production. CONCLUSION:Carvedilol appears to have a non-specific effect on membranes and to interfere with the phospholipase D signaling pathway, with subsequent inhibition of reactive oxygen species generation and myeloperoxidase release, without affecting iNOS expression.






Pecivová J,Macicková T,Lojek A,Gallova L,Cíz M,Nosál' R,Holománová D




Has Abstract


2007-01-01 00:00:00














  • Modulation of urokinase-type plasminogen activator by transforming growth factor beta1 in acetaldehyde-activated hepatic stellate cells.

    abstract::The aim of this study was to determine whether transforming growth factor-beta1 (TGF-beta1) induces the synthesis, release and gene expression of urokinase-type plasminogen activator (uPA) in hepatic stellate cells. In addition to stimulating collagen production, TGF-beta1 induced the morphological and phenotypical ch...


    pub_type: 杂志文章


    authors: Pérez-Liz G,Flores-Hernández J,Arias-Montaño JA,Reyes-Esparza JA,Rodríguez-Fragoso L

    更新日期:2005-01-01 00:00:00

  • Sucralfate and gastric bicarbonate.

    abstract::The effect of sucralfate treatment (3 g daily for 6 weeks) on gastric HCO3- secretion was investigated in 15 duodenal ulcer patients. After treatment a highly significant (p less than 0.01) increase in gastric bicarbonate output was found. It is suggested that the mode of action of sucralfate includes stimulation of g...


    pub_type: 杂志文章


    authors: Guslandi M

    更新日期:1985-01-01 00:00:00

  • Anomalous results of studies on drug interaction in man. II. Halofenate (mk-185) and antipyrine, bishydroxycoumarin, and warfarin.

    abstract::Three highly reproducible experiments on drug interaction in normal human volunteers provided anomalous results: chronic halofenate administration shortened plasma antipyrine and bishydroxycoumarin half-lives but prolonged plasma warfarin half-lives. This dissociation in the effect produced by a chronically administer...


    pub_type: 杂志文章


    authors: Vesell ES,Passananti GT

    更新日期:1975-01-01 00:00:00

  • Korean Red Ginseng Inhibits Amyloid-β-Induced Apoptosis and Nucling Expression in Human Neuronal Cells.

    abstract:INTRODUCTION:The accumulation of amyloid-β (Aβ) plaque in the brain is a characteristic feature of Alzheimer's disease (AD) and the cause of fatal oxidative damage to neuronal cells. Korean red ginseng (RG) is used extensively in traditional medicine and is known to have anti-oxidative and anti-inflammatory properties....


    pub_type: 杂志文章


    authors: Choi S,Lim JW,Kim H

    更新日期:2020-01-01 00:00:00

  • The Small Molecule Indirubin-3'-Oxime Inhibits Protein Kinase R: Antiapoptotic and Antioxidant Effect in Rat Cardiac Myocytes.

    abstract::Double-stranded, RNA-dependent protein kinase R (PKR) is a serine/threonine protein kinase activated by various stress signals. It plays an important role in inflammation, insulin sensitivity and glucose homeostasis. Increased PKR activity has been observed in obese humans as well as in obese diabetic mice. Indirubin-...


    pub_type: 杂志文章


    authors: Udumula MP,Medapi B,Dhar I,Bhat A,Desai K,Sriram D,Dhar A

    更新日期:2016-01-01 00:00:00

  • Participation of neuropeptides in antigen-induced contraction of guinea pig bronchi via NK(2) but not NK(1) receptor stimulation.

    abstract::We evaluated the contribution of neuropeptides to antigen-induced contractions of isolated bronchi and tracheae of passively sensitized guinea pigs using CP-96345 and SR 48968, specific antagonists of NK(1) and NK(2) receptors, respectively, in combination with treatment by an antihistaminic and a cysteinyl leukotrien...


    pub_type: 杂志文章


    authors: Tanpo T,Nabe T,Yasui K,Kamiki T,Kohno S

    更新日期:2000-05-01 00:00:00

  • Influence of the 2'-chloro substitution on central activity of desmethyldiazepam.

    abstract::Two diazepam derivatives, the N-desmethyl and the 2'-chlor-N-desmethyldiazepam, were compared in rats using behavioral and EEG techniques. Both drugs had depressant effects upon locomotor activity, facilitated behavior suppressed by punishment, increased the number of shocks received by rats in a Sidman avoidance proc...


    pub_type: 杂志文章


    authors: Babbini M,Gaiardi M,Bartoletti M

    更新日期:1978-01-01 00:00:00

  • Hemodynamic effects of carvedilol infusion and the contribution of the sympathetic nervous system in rats with heart failure.

    abstract::We investigated the contribution of the sympathetic nervous system (SNS) in maintaining the blood pressure and in regulating the cardiac function during and after carvedilol administration in rats with heart failure (group F). Left ventricular end-diastolic pressure, percent functional shortening, and rates of intrave...


    pub_type: 杂志文章


    authors: Abe Y,Watanabe K,Sato S,Nagai Y,Kamal FA,Wahed MI,Wen J,Narasimman G,Ma M,Suresh P,Takahashi T,Tachikawa H,Kashimura T,Tanabe N,Kodama M,Aizawa Y,Yamaguchi K,Miyazaki M,Kakemi M

    更新日期:2004-12-01 00:00:00

  • Antiarrhythmic-like actions of the smooth muscle spasmolytic agent, cinnamedrine, on action potentials of mammalian ventricular tissue.

    abstract::Cinnamedrine is an active ingredient in preparations used to relieve dysmenorrhea. It has been reported to have local anesthetic properties in nerve. This property prompted us to evaluate the effects of cinnamedrine on the cardiac action potential. Cinnamedrine (10-35 microM) significantly reduced the overshoot and ma...


    pub_type: 杂志文章


    authors: Arena JP,McArdle JJ,Argentieri TM

    更新日期:1987-01-01 00:00:00

  • Therapeutic drug monitoring of the HIV/AIDS drugs abacavir, zidovudine, efavirenz, nevirapine, indinavir, lopinavir, and nelfinavir.

    abstract::Combination therapy with antiretroviral drugs is used for the treatment of patients infected with the human immunodeficiency virus. To achieve optimal drug concentrations for viral suppression and avoidance of drug toxicity, monitoring of drug levels has been considered essential. We set up an analytical procedure for...


    pub_type: 杂志文章


    authors: Donnerer J,Kronawetter M,Kapper A,Haas I,Kessler HH

    更新日期:2003-12-01 00:00:00

  • Protective effects of sodium daidzein sulfonate on trabecular bone in ovariectomized rats.

    abstract::The ovariectomized (OVX) rat, as an established animal model of human osteoporosis, was adopted in the present experiment to study the protective effects of sodium daidzein sulfonate (SDS) on trabecular bone. Six-month-old Sprague-Dawley rats were sham-operated or ovariectomized. Five days later, the OVX rats were ran...


    pub_type: 杂志文章


    authors: Ren P,Ji H,Shao Q,Chen X,Han J,Sun Y

    更新日期:2007-01-01 00:00:00

  • Trichostatin A Induces Autophagy in Cervical Cancer Cells by Regulating the PRMT5-STC1-TRPV6-JNK Pathway.

    abstract:OBJECTIVE:The aim of this study was to investigate the effects of trichostatin A (TSA) on cervical cancer and the related mechanisms. METHODS:The HeLa and Caski cervical cancer cell lines were treated with different concentrations of TSA. Cell viability was measured by MTT assays. Cell apoptosis was analysed using flo...


    pub_type: 杂志文章


    authors: Liu JH,Cao YM,Rong ZP,Ding J,Pan X

    更新日期:2020-11-03 00:00:00

  • Fluid resuscitation improves survival of endotoxemic or septicemic rats: possible contribution of tumor necrosis factor.

    abstract::The present study was designed to investigate the effects of fluid administration on survival in endotoxemic or septicemic male Sprague-Dawley rats. Endotoxemia was induced by intravenous injection of Escherichia coli lipopolysaccharide (LPS), and septicemia produced by cecal ligation and puncture (CLP). In endotoxemi...


    pub_type: 杂志文章


    authors: Smith EF 3rd,Slivjak MJ,Egan JW,Gagnon R,Arleth AJ,Esser KM

    更新日期:1993-05-01 00:00:00

  • A new model for the study of opiate B-receptors: isolated splenic strip of the cat.

    abstract::Noradrenergic neurotransmission in the electrically stimulated isolated splenic strip of the cat was found to be modulated via opiate receptor. Pharmacodynamic parameters of morphine, methionine-enkephalin, azidomorphine, naloxone and N-cyclopropylmethyl-norazidomorphine (CAM) were determined. As CAM proved to be more...


    pub_type: 杂志文章


    authors: Kerecsen L,Knoll J

    更新日期:1981-01-01 00:00:00

  • Protective effect of verapamil upon ouabain-induced cardiac arrhythmias.

    abstract::Protective influence of verapamil upon ouabain-induced cardiac arrhythmias was investigated in anesthetized (alpha-chloralose 60 mg/kg and urethane 500 mg/kg) open-chest guinea pigs. Verapamil in doses between 100 and 150 micrograms/kg significantly increased (80-90%) the dose of ouabain, necessary to cause ventricula...


    pub_type: 杂志文章


    authors: Khatter JC,Navaratnam S,Hoeschen RJ

    更新日期:1988-01-01 00:00:00

  • Effect of atorvastatin treatment on isoproterenol-induced myocardial infarction in rats.

    abstract::In the present study, chronic treatment of atorvastatin was evaluated on isoproterenol-induced myocardial infarction. Male Sprague-Dawley rats (200 +/- 25 g) were randomized into the following four groups: (1) control group, (2) isoproterenol-treated group, (3) atorvastatin-treated group, and (4) isoproterenol- and at...


    pub_type: 杂志文章


    authors: Trivedi CJ,Balaraman R,Majithiya JB,Bothara SB

    更新日期:2006-01-01 00:00:00

  • Aspirin, indomethacin, and tartrazine increase carotid-sinus-nerve activity and arterial blood pressure in guinea pigs.

    abstract::Acetylsalicylic acid (ASA; 0.1-10 mg/kg), indomethacin (IND; 0.1-1.0 mg/kg), and tartrazine (TZ; 0.1-2.0 mg/kg), given intravenously induced dose-dependent increases in carotid-sinus nerve (CSN) activity, accompanied by increases in mean arterial blood pressure (MABP), but only the IND-induced MABP increases were dose...


    pub_type: 杂志文章


    authors: D'Souza SJ,Biggs DF

    更新日期:1987-01-01 00:00:00

  • Effect of anti-parkinsonism drugs on gastric emptying and intestinal transit in the rat.

    abstract::The effect of atropine sulfate, trihexyphenidyl HCl, benztropine mesylate, diphenhydramine HCl and ethopropazine HCl on gastric emptying and intestinal transit of a phenol red solution in the rat was examined. Intraperitoneal administration of 0.3 mg/kg atropine, 1.2 mg/kg benztropine and trihexyphenidyl results in a ...


    pub_type: 杂志文章


    authors: Feldman S,Putcha L

    更新日期:1977-01-01 00:00:00

  • Study of the vasodilating activity of salbutamol on dog coronary arteries. Unexpected effects of methylene blue.

    abstract::The vascular relaxant effect of salbutamol and its dependence on the endothelium were studied in the isolated dog coronary artery, precontracted with prostaglandin F2 alpha. Salbutamol induced a concentration-dependent relaxation which was partially inhibited by removal of endothelial cells. Atenolol 10(-6) mol/l, a b...


    pub_type: 杂志文章


    authors: Bernard F,Jouquey S,Hamon G

    更新日期:1991-01-01 00:00:00

  • Hepatic cytochrome P-450-dependent metabolism and enzymatic conjugation of foreign compounds in vitamin A-deficient rats.

    abstract::The temporal effects of vitamin A deficiency on hepatic cytochrome P-450-dependent and conjugation reactions were studied in the rat. Cytochrome P-450 levels and N-methyl-p-chloroaniline N-demethylase activity were significantly reduced in the deficient animals. No other changes in parameters dependent on cytochrome P...


    pub_type: 杂志文章


    authors: Siddik ZH,Drew R,Litterst CL,Mimnaugh EG,Sikic BI,Gram TE

    更新日期:1980-01-01 00:00:00

  • Age-dependent changes in the myocardial adenylate cyclase of normotensive and spontaneously hypertensive rat.

    abstract::The activity of myocardial adenylate cyclase from two age groups (10-11 and 18-19 weeks old, respectively) of male spontaneously hypertensive rats (SHR) and their age-matched normotensive Wistar-Kyoto rats (WKY) was examined. Stimulation of the enzyme by isoproterenol and 5'-guanylyl-imidodiphosphate (GMP-PNP) was dec...


    pub_type: 杂志文章


    authors: Chiu TH

    更新日期:1981-01-01 00:00:00

  • Differential treatment regimen-related effects of HU210 on CB(1) and D(2)-like receptor functionality in the rat basal ganglia.

    abstract:BACKGROUND/AIMS:Functional linkages between the cannabinoid CB(1) and the dopaminergic systems have been reported although the observations and the mechanisms hypothesizing their interactions at the G protein-coupled receptor (GPCR) functionality level are conflicting. METHODS:Administration of a potent cannabinoid ag...


    pub_type: 杂志文章


    authors: Nguyen VH,Wang H,Verdurand M,Zavitsanou K

    更新日期:2012-01-01 00:00:00

  • Involvement of the nitric oxide/cyclic guanylate monophosphate pathway in the pilocarpine-induced seizure model in mice.

    abstract::The present study was designed to investigate the involvement of the nitric oxide (NO)/cyclic guanylate monophosphate pathway in pilocarpine-induced seizures in mice. Male Swiss mice (26-32 g) were used as the in vivo model. The following pharmacological tools were utilized: the non-selective NO synthase (NOS) inhibit...


    pub_type: 杂志文章


    authors: Vasconcelos Rios ER,Moura Rocha NF,Rodrigues Carvalho AM,Freire Vasconcelos L,Leite Dias M,de Carvalho Lima CN,Soares Lopes K,Cavalcante Melo FH,de França Fonteles MM

    更新日期:2013-01-01 00:00:00

  • Euodia daniellii Hemsl. (Bee-Bee Tree) Oil Attenuates Palmitate-Induced Lipid Accumulation and Apoptosis in Hepatocytes.

    abstract::Hepatic lipid accumulation and apoptosis is elevated in patients with non-alcoholic steatohepatitis and is closely associated with severity. Saturated fatty acid palmitate stimulates lipid accumulation and apoptosis in hepatocytes. In the present study, we examined bee-bee tree oil (BO)-mediated protective effects on ...


    pub_type: 杂志文章


    authors: Jung TW,Kim SY,Kim DS,Shin EC,Park YB,Lee KT

    更新日期:2018-01-01 00:00:00

  • Clofibrate decreases jejunal cholesterol synthesis and activity of postheparin plasma lipoprotein lipase in the rat.

    abstract::Clofibrate treatment decreased drastically the activity of serum postheparin lipoprotein lipase of rats fed with fat-free test diet but had no effect on hepatic lipase. 3 weeks' treatment was followed by a significant fall in serum cholesterol caused by a marked decrease in HDL-cholesterol. Clofibrate decreased in vit...


    pub_type: 杂志文章


    authors: Strandberg TE,Kuusi T,Tilvis RS,Miettinen TA

    更新日期:1983-01-01 00:00:00

  • Monophosphoryl lipid A preserves myocardial contractile function following multiple, brief periods of coronary occlusion in dogs.

    abstract::The effects of a 1- or 24-hour pretreatment regimen with monophosphoryl lipid A (MLA, 35 micrograms/kg i.v.) on myocardial stunning produced by repetitive coronary occlusions were studied in barbital-anesthetized dogs. Regional segment function (%SS) and myocardial blood flow were measured by sonomicrometry and the ra...


    pub_type: 杂志文章


    authors: Yao Z,Elliott GT,Gross GJ

    更新日期:1995-09-01 00:00:00

  • Cannabinoid receptor agonists inhibit Ca(2+) influx to synaptosomes from rat brain.

    abstract::We examined the effects of cannabinoid receptor agonists on (45)Ca(2+) uptake in rat brain synaptosomes. A cannabinoid receptor agonist, (R)-(+)-[2,3-dihydro-5-methyl-3-[(4-merpholino)methyl]pyrrolo-[1,2,3-de]-1,4-benzoxazin-6-yl](1-naphthyl)methanone (WIN 55212-2) dose-dependently inhibited (45)Ca(2+) uptake in rat s...


    pub_type: 杂志文章


    authors: Yoshihara S,Morimoto H,Ohori M,Yamada Y,Abe T,Arisaka O

    更新日期:2006-01-01 00:00:00

  • Pharmacokinetics of two novel bicalutamide formulations in healthy male volunteers.

    abstract::The oral bioavailability of two investigational formulations of bicalutamide was compared with the current clinical formulation. The first formulation was amorphous R-/S-bicalutamide in solid dispersion with a polymeric matrix of hydroxypropyl methylcellulose phthalate (HP55S) (R-/S-bicalutamide/HP55S); the second was...


    pub_type: 杂志文章,随机对照试验


    authors: Cantarini M,Fuhr R,Morris T

    更新日期:2006-01-01 00:00:00

  • Effects of fructose-1,6-diphosphate on the activity of rat liver nitric oxide synthase in vitro.

    abstract::Fructose-1,6-diphosphate (FDP) was found to cause significant stimulation of nitric oxide synthase (NOS) in rat liver homogenates in vitro. This effect was more pronounced for the inducible isoform than its constitutive counterpart. Furthermore, FDP restored rat liver inducible NOS levels following their depletion by ...


    pub_type: 杂志文章


    authors: Mihas AA,Maliakkal RJ,Mihas TA,Kanji VK,Markov AK

    更新日期:1997-01-01 00:00:00

  • Development of an intravenous desferrioxamine mesylate treatment protocol for swine: monitoring of desferrioxamine and metabolites by high-performance liquid chromatography.

    abstract::Desferrioxamine (DFO) metabolism and its pharmacokinetics were studied in a swine model using high-performance liquid chromatography. DFO and three iron-binding metabolites occurred in plasma. Interindividual differences in pharmacokinetics and metabolism were observed. Urine analysis in 4 pigs showed three iron-bindi...


    pub_type: 杂志文章


    authors: Fisher EA,McLachlan DR,Kruck TP,Mustard RA

    更新日期:1990-01-01 00:00:00