Abstract:
:GPR61 is an orphan receptor that belongs to Class A of G-protein-coupled receptors. It has been reported that GPR61 has a constitutive activity and couples to Gαs. In the present study, we characterized GPR61 function and ligand binding by experimental and molecular docking studies. We demonstrated that heterologous expression of GPR61 in HEK293 cells enhanced the cAMP synthesis response to forskolin, whereas the basal cAMP synthesis was unaffected. 5-(Nonyloxy)tryptamine inhibited forskolin-stimulated cAMP production in GPR61-expressing HEK293 cells. These studies highlight that the intrinsic activity of this receptor is only measurable following its synergy with Gαs.
journal_name
Pharmacologyjournal_title
Pharmacologyauthors
Kozielewicz P,Grafton G,Sajkowska-Kozielewicz JJ,Barnes NMdoi
10.1159/000501926subject
Has Abstractpub_date
2019-01-01 00:00:00pages
377-382issue
5-6eissn
0031-7012issn
1423-0313pii
000501926journal_volume
104pub_type
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