Alkaline comet assay study with breast cancer patients: evaluation of baseline and chemotherapy-induced DNA damage in non-target cells.


:The sensitivity of the alkaline comet assay for the evaluation of baseline and treatment-induced DNA damage in white blood cells of breast cancer patients receiving adjuvant chemotherapy according to three conventional anthracycline- and cyclophosphamide-containing protocols was investigated. Additionally, baseline DNA damage in cancer patients was compared with the levels of DNA damage recorded in healthy women. Altogether 30 patients with diagnosed breast cancer and 30 female blood donors with no known familial history of breast cancer participated in the study. Alkaline comet assay was performed according to standard protocol and DNA migration in peripheral blood leukocytes was measured by a computer-based image analysis system. For each subject the frequency of "damaged" cells, i.e., long-tailed nuclei (LTN) with tail length exceeding 95th percentile for the considered parameter among controls, is also reported. Breast cancer patients had significantly increased background levels of DNA damage in their peripheral blood leukocytes as compared to healthy women. Prior to the chemotherapy, a majority of patients showed a statistically significant increase in the number of LTN compared to healthy blood donors. Marked interindividual variations in baseline DNA damage among patients were recorded, some of them related to the disease stage and status. The present study confirmed the alkaline comet assay as a sensitive technique able to detect significantly elevated DNA migration in blood cells of patients already one hour after completion of the first cycle of chemotherapy. Administration of antineoplastic drugs in three chemotherapy protocols studied induced a similar increase of primary DNA damage in nontarget cells. The evaluation of the LTN frequencies indicates the best response to the protocol containing cyclophosphamide, methotrexate and 5-fluorouracil (CMF). Our results point to the significance of simultaneous evaluation of DNA migration and frequency of LTN in the same subject and approved the use of alkaline comet assay as a suitable method for the routine detection of critical DNA lesions produced after administration of antineoplastic drugs in the clinical settings.


Clin Exp Med


Kopjar N,Milas I,Garaj-Vrhovac V,Gamulin M




Has Abstract


2006-12-01 00:00:00












  • Nitric oxide production by Leishmania-infected macrophages and modulation by prostaglandin E2.

    abstract::Nitric oxide (NO), produced by the nitric oxide synthase (iNOS) enzyme, is the most-important molecule responsible for the killing of Leishmania parasites by macrophages. In previous work we have demonstrated that, after activation with recombinant human interferon-gamma and/or bacterial lipopolysaccharide, human macr...

    journal_title:Clinical and experimental medicine

    pub_type: 杂志文章


    authors: Panaro MA,Brandonisio O,Sisto M,Acquafredda A,Leogrande D,Fumarola L,Mitolo V

    更新日期:2001-09-01 00:00:00

  • DNA methylation and not allelic variation regulates STAT6 expression in human T cells.

    abstract::STAT6 transcription factor, which has been implicated in commitment to Th2, is known to be activated by IL-4 and IL-13. Accordingly, STAT6 is primarily responsible for the transcriptional effects of IL-4 and IL-13. STAT6-deficient mice are known to have defective IL-4-mediated functions, such as B cell proliferation, ...

    journal_title:Clinical and experimental medicine

    pub_type: 杂志文章


    authors: Kim EG,Shin HJ,Lee CG,Park HY,Kim YK,Park HW,Cho SH,Min KU,Cho ML,Park SH,Lee CW

    更新日期:2010-09-01 00:00:00

  • Establishing serological classification tree model in rheumatoid arthritis using combination of MALDI-TOF-MS and magnetic beads.

    abstract::To establish a serological classification tree model for rheumatoid arthritis (RA), protein/peptide profiles of serum were detected by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF-MS) combined with weak cationic exchange (WCX) from Cohort 1, including 65 patients with RA and ...

    journal_title:Clinical and experimental medicine

    pub_type: 杂志文章


    authors: Yan Z,Chaojun H,Chuiwen D,Xiaomei L,Xin Z,Yongzhe L,Fengchun Z

    更新日期:2015-02-01 00:00:00

  • Hereditary periodic fever and reactive amyloidosis.

    abstract::Hereditary periodic fever syndromes (HPF) are a group of diseases characterised by recurrences of fever and inflammation separated by symptom-free intervals. Familial Mediterranean fever (FMF) is the most frequent entity within this group of disorders which further consists of hyperimmunoglobulinaemia D and periodic f...

    journal_title:Clinical and experimental medicine

    pub_type: 杂志文章,评审


    authors: van der Hilst JC,Simon A,Drenth JP

    更新日期:2005-10-01 00:00:00

  • FDG PET/CT as a survival prognostic factor in patients with advanced renal cell carcinoma.

    abstract::Accurate prediction of the outcome of molecular target-based treatment in advanced renal cell carcinoma (RCC) is an important clinical problem. Positron emission tomography/computed tomography using [18F]-2-fluoro-2-deoxyglucose (FDG PET/CT) is a noninvasive tool for the assessment of glucose accumulation which can be...

    journal_title:Clinical and experimental medicine

    pub_type: 杂志文章


    authors: Pankowska V,Malkowski B,Wedrowski M,Wedrowska E,Roszkowski K

    更新日期:2019-02-01 00:00:00

  • Six long noncoding RNAs as potentially biomarkers involved in competitive endogenous RNA of hepatocellular carcinoma.

    abstract::To investigate lncRNAs acting as competing endogenous RNAs (ceRNAs) involved in oncogenesis and progression of HCC. Different expressed lncRNAs, microRNAs, and mRNAs (DElncRNAs, DEmiRNAs, DEmRNAs), downloaded from The Cancer Genome Atlas (TCGA) database, were identified by edgeR package. CeRNA network was constructed ...

    journal_title:Clinical and experimental medicine

    pub_type: 杂志文章


    authors: Qu L,Cai X,Xu J,Wei X,Qu X,Sun L,Gong L,Su C,Zhu Y

    更新日期:2020-08-01 00:00:00

  • Telomere length in non-neoplastic colonic mucosa in ulcerative colitis (UC) and its relationship to the severe clinical phenotypes.

    abstract::Telomere shortening occurs with human aging in many organs and tissues and is accelerated by rapid cell turnover and oxidative injury. To clarify the clinical importance of telomere shortening in colonic mucosa in ulcerative colitis (UC), we measured average telomere length using quantitative real-time PCR in non-neop...

    journal_title:Clinical and experimental medicine

    pub_type: 杂志文章


    authors: Tahara T,Shibata T,Okubo M,Kawamura T,Sumi K,Ishizuka T,Nakamura M,Nagasaka M,Nakagawa Y,Ohmiya N,Arisawa T,Hirata I

    更新日期:2015-08-01 00:00:00

  • Structural changes in alpha1-adrenoceptor antagonist-treated human prostatic stroma.

    abstract::Alpha1-adrenoceptor antagonists (alpha1-blockers) are currently used as first-line drugs for the treatment of benign prostatic hyperplasia (BPH). However, cases of BPH are often encountered in which the efficacy of alpha1-blockers decreases and switching to surgical treatment is required. One factor responsible for th...

    journal_title:Clinical and experimental medicine

    pub_type: 杂志文章


    authors: Imamura T,Ishii K,Kanda H,Arase S,Yoshio Y,Hori Y,Soga N,Kise H,Arima K,Sugimura Y

    更新日期:2010-06-01 00:00:00

  • Slit2 is a potential biomarker for renal impairment in systemic lupus erythematosus.

    abstract::Slit2 glycoprotein has been described to regulate the inflammatory response and be involved in autoimmune diseases. Here, we investigated the expression of Slit2 and its potential significance in systemic lupus erythematosus (SLE). A total of 103 patients with SLE participated in our study. The levels of serum Slit2 w...

    journal_title:Clinical and experimental medicine

    pub_type: 杂志文章


    authors: Zhang Y,Hu L,Li X,Chen L,Yang X

    更新日期:2020-10-20 00:00:00

  • The oxidant and antioxidant effects of 25-hydroxyvitamin D3 in liver, kidney and heart tissues of diabetic rats.

    abstract::Previous studies have implicated protective effects of vitamin D on insulin secretion and pancreas beta cell function. The goal of the present study is to determine if a combination therapy of 25-hydroxyvitamin D3 and insulin had any advantage over insulin therapy alone on lipid peroxidation and antioxidant activity i...

    journal_title:Clinical and experimental medicine

    pub_type: 杂志文章


    authors: Noyan T,Balaharoğlu R,Kömüroğlu U

    更新日期:2005-05-01 00:00:00

  • 1q23.1 homozygous deletion and downregulation of Fc receptor-like family genes confer poor prognosis in chronic lymphocytic leukemia.

    abstract::The identification of chromosome 1 translocations and deletions is a rare and poorly investigated event in chronic lymphocytic leukemia (CLL). Nevertheless, the identification of novel additional molecular alterations is of great interest, opening to new prognostic and therapeutic strategies for such heterogeneous hem...

    journal_title:Clinical and experimental medicine

    pub_type: 杂志文章


    authors: Daniele G,L'Abbate A,Turchiano A,Palumbo O,Carella M,Lo Cunsolo C,Iuzzolino P,Lonoce A,Hernández-Sánchez M,Minoia C,Leone P,Hernandez-Rivas JM,Storlazzi CT

    更新日期:2019-05-01 00:00:00

  • Tumor-resident adenosine-producing mesenchymal stem cells as a potential target for cancer treatment.

    abstract::The development of new therapies based on tumor biology is one of the main topics in cancer treatment. In this regard, investigating the microenvironment and cellular composition of the tumor is of particular interest. Mesenchymal stem cells (MSCs) are a major group of cells in the tumor tissue and play a critical rol...

    journal_title:Clinical and experimental medicine

    pub_type: 杂志文章,评审


    authors: Arab S,Alizadeh A,Asgharzade S

    更新日期:2021-01-23 00:00:00

  • Effect of allogeneic bone marrow-derived mesenchymal stem cells transplantation in a polyI:C-induced primary biliary cirrhosis mouse model.

    abstract::Primary biliary cirrhosis (PBC) is a slowly progressive autoimmune disease of unknown mechanism. We established a PBC animal model by injecting C57BL/6 mice with polyinosinic-polycytidylic acid sodium (polyI:C) to investigate the therapeutic effect of bone marrow-derived mesenchymal stem cells (BM-MSC) on this model. ...

    journal_title:Clinical and experimental medicine

    pub_type: 杂志文章


    authors: Wang D,Zhang H,Liang J,Gu Z,Ma X,Huang J,Lin J,Hou Y,Lu L,Sun L

    更新日期:2011-03-01 00:00:00

  • Autoantibodies to the ribosomal P proteins in systemic lupus erythematosus.

    abstract::This paper describes the clinical significance of antibodies to the ribosomal P proteins in systemic lupus erythematosus. It appears that liver disease due to the lupus process and not attributable to viral infection, alcohol or drugs is associated with anti-ribosomal P. In addition, there is a strong relationship to ...

    journal_title:Clinical and experimental medicine

    pub_type: 杂志文章,评审


    authors: Reichlin M

    更新日期:2006-06-01 00:00:00

  • The effect of rhBMP-2 on pulmonary arterioles remodeling in endotoxin-induced acute lung injury in rats.

    abstract::Endotoxin is known to cause acute lung injury (ALI). Here, we investigated the effects and mechanisms of recombinant human bone morphogenetic protein-2 (rhBMP-2) on pulmonary arteriole remodeling in endotoxin-induced ALI in rats. Sixty Wistar rats were randomly divided into 3 groups (n = 20). Control group was infused...

    journal_title:Clinical and experimental medicine

    pub_type: 杂志文章


    authors: Wang K,Gong J,Pei L,Shan S,Tan W

    更新日期:2013-08-01 00:00:00

  • Recombinant human interferon gamma (Gamma Immunex) in treatment of atopic dermatitis.

    abstract::Atopic dermatitis (AD) is a chronic, inflammatory skin disease which is characterized by severe pruritus and affects patients' quality of life. In recent years gamma interferon (IFN-gamma) has been accepted as a novel treatment for severe AD, however, its mechanism of action is not clearly identified. Present study ev...

    journal_title:Clinical and experimental medicine

    pub_type: 临床试验,杂志文章


    authors: Panahi Y,Davoudi SM,Madanchi N,Abolhasani E

    更新日期:2012-12-01 00:00:00

  • Eculizumab in refractory catastrophic antiphospholipid syndrome: a case report and systematic review of the literature.

    abstract::Catastrophic antiphospholipid syndrome (CAPS) is a rare disorder, characterized by the development of multiple vascular thrombosis over a short period of time, in patients with persistently detectable antiphospholipid antibodies (aPLs). The vascular occlusions predominantly affect small vessels. The overall mortality ...

    journal_title:Clinical and experimental medicine

    pub_type: 杂志文章


    authors: Tinti MG,Carnevale V,Inglese M,Molinaro F,Bernal M,Migliore A,De Cata A

    更新日期:2019-08-01 00:00:00

  • Early detection of RhD status in pregnancies at risk of hemolytic disease of the newborn.

    abstract::The aim of this work was to investigate the presence of the RHD gene in fetal cells obtained from amniotic fluid. We studied 65 samples of amniotic fluid, 11 from RhD-negative mothers sensitized with anti-D alloantibodies. The fetal origin of the DNA was confirmed with the analysis of 1 VNTR locus and 3 STR loci in DN...

    journal_title:Clinical and experimental medicine

    pub_type: 杂志文章


    authors: Cotorruelo C,Biondi C,García Borrás S,Di Mónaco R,Racca A

    更新日期:2002-07-01 00:00:00

  • Takayasu arteritis: a cohort of Italian patients and recent pathogenetic and therapeutic advances.

    abstract::Takayasu arteritis (TAK) is a rare granulomatous vasculitis of unknown etiology that mainly affects the aorta and its major branches. The aim is to describe the clinical features, diagnostic procedures, pathogenesis, and management of TAK in a longitudinal cohort of patients recruited within a single region of souther...

    journal_title:Clinical and experimental medicine

    pub_type: 杂志文章


    authors: Dammacco F,Cirulli A,Simeone A,Leone P,Pulli R,Angiletta D,Rubini G,Di Palo A,Vacca A,Dammacco R

    更新日期:2020-10-07 00:00:00

  • High mobility group box 1 induces synoviocyte proliferation in rheumatoid arthritis by activating the signal transducer and activator transcription signal pathway.

    abstract::Recently, it was discovered that high mobility group box chromosomal protein 1 (HMGB1) acts as a potent pro-inflammatory cytokine that is released into the extracellular milieu. Signal transducer and activator of transcription (STAT) proteins play an important role in cytokine signaling. Some investigators have report...

    journal_title:Clinical and experimental medicine

    pub_type: 杂志文章


    authors: Guo HF,Liu SX,Zhang YJ,Liu QJ,Hao J,Gao LX

    更新日期:2011-06-01 00:00:00

  • Paralytic ileus following "subcutaneous bortezomib" therapy: focus on the clinical emergency-report of two cases.

    abstract::We retrospectively analyzed the medical history of 19 elderly myeloma patients treated with the "novel subcutaneous formulation of bortezomib." In our experience, two patients (10 %) discontinued treatment for paralytic ileus. The exact pathogenetic mechanisms of toxic megacolon and paralytic ileus due to "novel subcu...

    journal_title:Clinical and experimental medicine

    pub_type: 杂志文章


    authors: Mele G,Coppi MR,Melpignano A,Quarta G

    更新日期:2016-02-01 00:00:00

  • Leukocytes apoptosis and adipocytokines in children with beta thalassemia major.

    abstract::β-Thalassemia is a significant public health problem in Egypt. Infectious complications represent the second most common cause of mortality and the major cause of morbidity in β-thalassemia major (BTM). The increased susceptibility of these patients to infectious diseases has been attributed to the abnormalities of th...

    journal_title:Clinical and experimental medicine

    pub_type: 杂志文章


    authors: Elsayh KI,Mohammed WS,Zahran AM,Saad K

    更新日期:2016-08-01 00:00:00

  • Diverse phenotypic and genotypic presentation of RAG1 mutations in two cases with SCID.

    abstract::Severe combined immunodeficiencies (SCID) comprise a spectrum of genetic defects that involve both humoral and cellular immunities. Defects in recombinating activating gene 1 (RAG1), RAG2, Artemis, or LIG4 can disrupt V(D)J recombination. Defective V(D)J recombination of the T and B cell receptors is responsible for T...

    journal_title:Clinical and experimental medicine

    pub_type: 杂志文章


    authors: Karaca NE,Aksu G,Genel F,Gulez N,Can S,Aydinok Y,Aksoylar S,Karaca E,Altuglu I,Kutukculer N

    更新日期:2009-12-01 00:00:00

  • Assessment of CD40 and CD40L expression in rheumatoid arthritis patients, association with clinical features and DAS28.

    abstract::The predominance of the effector mechanisms by CD4 + T cells is a characteristic of inflammatory autoimmune diseases such as rheumatoid arthritis (RA). The CD40/CD40L costimulatory pathway contributes to these pathogenic mechanisms by promoting autoantibody production and inflammation. Aberrant expression of CD40 and ...

    journal_title:Clinical and experimental medicine

    pub_type: 杂志文章


    authors: Román-Fernández IV,García-Chagollán M,Cerpa-Cruz S,Jave-Suárez LF,Palafox-Sánchez CA,García-Arellano S,Sánchez-Zuno GA,Muñoz-Valle JF

    更新日期:2019-11-01 00:00:00

  • Parathormone and 1,25(OH)2D3 but not 25(OH)D3 serum levels, in an inverse correlation, reveal an association with advanced stages of colorectal cancer.

    abstract::Experimental, epidemiologic, and clinical trial data indicate the antineoplastic effects of calcium and vitamin D in large-bowel neoplasia. The aim of this study was to determine serum levels of vitamin D metabolites and parathormone (PTH) in patients with colorectal cancer (CRC) and to extract conclusions comparing t...

    journal_title:Clinical and experimental medicine

    pub_type: 杂志文章


    authors: Charalampopoulos A,Charalabopoulos A,Batistatou A,Golias C,Anogeianaki A,Peschos D,Iliadis I,Macheras A,Charalabopoulos K

    更新日期:2010-03-01 00:00:00

  • In vitro autoreactivity against skin in rheumatoid arthritis: are peripheral blood mononuclear cells of patients with rheumatoid arthritis able to lyze autologous keratinocytes?

    abstract::An in vitro skin explant model was originally developed to predict the occurrence and severity of acute graft-versus-host disease in allogeneic hematopoietic stem cell transplants. In previous studies we reported that peripheral blood mononuclear cells of patients with rheumatoid arthritis were able to induce graft-ve...

    journal_title:Clinical and experimental medicine

    pub_type: 杂志文章


    authors: Stechová K,Vavrincová P,Reitzová H,Chudoba D,Zimák J,Hromadníková I

    更新日期:2001-06-01 00:00:00

  • Significance of persistence of antibodies against Leishmania infantum in Sicilian patients affected by acute visceral leishmaniasis.

    abstract::The background of this article is as follows: Few data are available about the persistence of serum-specific IgG antibodies to L. infantum after acute VL. The objective of this article is to evaluate the persistence of antibodies against L. infantum in patients healed from acute VL, and the kinetic of the same antibod...

    journal_title:Clinical and experimental medicine

    pub_type: 杂志文章


    authors: Mansueto P,Pepe I,Seidita A,Scozzari F,Vitale G,Arcoleo F,Elvira I,Cillari E,Rini GB,Napoli N,Di Rosa S,Mansueto S,Di Fede G

    更新日期:2012-06-01 00:00:00

  • Fuzzy logic-based tumor marker profiles improved sensitivity of the detection of progression in small-cell lung cancer patients.

    abstract::Tumor markers were used for disease monitoring in small-cell lung cancer patients. The aim of this study was to improve diagnostic efficiency in the detection of tumor progression in small-cell lung cancer patients by using fuzzy logic modeling in combination with a tumor marker panel (NSE, ProGRP, Tumor M2-PK, CYFRA ...

    journal_title:Clinical and experimental medicine

    pub_type: 杂志文章


    authors: Schneider J,Peltri G,Bitterlich N,Philipp M,Velcovsky HG,Morr H,Katz N,Eigenbrodt E

    更新日期:2003-02-01 00:00:00

  • Injection of basic fibroblast growth factor together with adipose-derived stem cell transplantation: improved cardiac remodeling and function in myocardial infarction.

    abstract::Recent findings suggest that cell and gene transplantation in the infarcted myocardium may improve heart function. The aim of the study was to investigate the mechanism involved in improving heart function following the co-injection of adipose-derived stem cells (ADSCs) and basic fibroblast growth factor (bFGF) in a r...

    journal_title:Clinical and experimental medicine

    pub_type: 杂志文章


    authors: Wang B,Ma X,Zhao L,Zhou X,Ma Y,Sun H,Yang Y,Chen B

    更新日期:2016-11-01 00:00:00

  • Mannose-binding lectin polymorphisms in common variable immunodeficiency.

    abstract::Common variable immunodeficiency (CVID) is a heterogeneous group of disorders, characterized by hypogammaglobulinemia and increased susceptibility to infections, autoimmunity and malignancies. This study was performed to analyze the Mannose-binding lectin (MBL) polymorphisms in Iranian patients with CVID. Thirty-five ...

    journal_title:Clinical and experimental medicine

    pub_type: 杂志文章


    authors: Aghamohammadi A,Foroughi F,Rezaei N,Dianat S,Solgi G,Amirzargar AA

    更新日期:2009-12-01 00:00:00