Abstract:
:The aim of this study was to evaluate the ferric-reducing ability of serum (FRAS), paraoxonase 1 (PON1), ceruloplasmin serum oxidase activity and hsCRP level in patients with type1 diabetes mellitus without and with diabetic retinopathy. The study was performed in 76 patients with type 1 diabetes mellitus, 35 without diabetic retinopathy (group 1) and 41 with preproliferative and proliferative retinopathy (group 2). Control group consisted of 35 nondiabetic, age-, gender-, body mass-matched healthy volunteers who came to the outpatient clinic for a routine health check-up. We evaluated FRAS using the method described by Benzie and Strain; PON1 by kinetic spectrophotometric assay with paraoxon as substrate and ceruloplasmin using its oxidative activity with 3-phenylenodiamine as substrate. CRP was measured with a high sensitive enzyme immunoassay. PON1 activity was significantly decreased in patients with diabetic retinopathy (227.66 +/- 123.57 U/l) when compared with control (312.04 +/- 129.77 U/l). FRAS was significantly decreased in group 2 (439.33 +/- 79.87 micromol/l) when compared with group 1 (522.79 +/- 167.56 micromol/l) and control (529.80 +/- 81.99 micromol/l). Ceruloplasmin activity was significantly elevated in group 1 (58.36 +/- 22.56 U/g protein) when compared with control (45.22 +/- 14.96 U/g protein). We have found significant increase in hsCRP level in group 2 (3.71 +/- 2.47 mg/l) when compared with group 1 (1.75 +/- 1.01 mg/l) and control (0.57 +/- 0.46 mg/l). The PON1/CRP ratio in control group was significantly increased when compared with diabetic patients and was significantly decreased in group 2 compared with group 1. We have not found gender-dependent difference in studied parameters in both control and in study groups. We have found tendency to decrease the serum activity of FRAS and hsCRP in elder patients but the difference was significant only in group 2. FRAS and PON 1 activity is decreased in patients with type 1 diabetes mellitus with presence of diabetic retinopathy which confirms that oxidative stress could play a role in pathogenesis of diabetic retinopathy. Significantly elevated levels of hsCRP in diabetic patients with the presence of diabetic retinopathy compared with patients without diabetic retinopathy providing a link between inflammation and the development of microvascular complication of diabetes. Because of the significant difference in PON1/CRP ratio between patients without and with the presence of diabetic retinopathy, it seems that PON1:CRP ratio may be used as a biochemical marker for progression of retinopathy. The link between the antioxidant concentration, inflammation and the development of diabetes complications needs further longitudinal studies in order to confirm our findings.
journal_name
Clin Exp Medjournal_title
Clinical and experimental medicineauthors
Nowak M,Wielkoszyński T,Marek B,Kos-Kudła B,Swietochowska E,Siemińska L,Karpe J,Kajdaniuk D,Głogowska-Szelag J,Nowak Kdoi
10.1007/s10238-009-0084-7subject
Has Abstractpub_date
2010-09-01 00:00:00pages
185-92issue
3eissn
1591-8890issn
1591-9528journal_volume
10pub_type
杂志文章abstract::The purpose of this study was to reveal the clinical diagnostic and prognostic value of miR-768-3p in HBV-related HCC and to investigate its effect on the biological function of HCC. Quantitative real-time polymerase chain reaction was used to detect the expression level of miR-768-3p in subjects' serum. The receiver ...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-020-00646-z
更新日期:2020-11-01 00:00:00
abstract::It is suggested that gastric mucins, and in particular some specific glycan structures that can act as carbohydrate receptors, are involved in the interactions with Helicobacter pylori adhesins. The main aim of our study was to evaluate glycosylation pattern of glycoproteins of gastric juice before and at the end of e...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-010-0113-6
更新日期:2011-06-01 00:00:00
abstract::Interleukin (IL)-27 is an IL-12 family cytokine and exerts a critical role in immune regulation in the context of infection, autoimmunity, and angiogenesis. In this study, we aimed to investigate the possible pathophysiological role of IL-27 and vascular endothelial growth factor (VEGF) in ankylosing spondylitis (AS)....
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-014-0281-x
更新日期:2015-05-01 00:00:00
abstract::Glioblastoma multiforme (GBM) is the most common malignancy in central nervous system. A small subpopulation of GBM cells known as GBM stem-like cells (GSLCs) were supposed to be the most malignant cells among GBM cells as they are resistant to multiple therapies including radiotherapy. In this study, we set up two GS...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-018-0504-7
更新日期:2018-08-01 00:00:00
abstract::Nasopharyngeal carcinoma (NPC) is a very common head and neck cancer in southern china. Despite advances in surgical and chemotherapeutic approaches, its prognosis is still not promising. Hedgehog signaling pathway was reported to be involved in a number of cancers including head and neck. However, it remains unclear ...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-012-0198-1
更新日期:2013-11-01 00:00:00
abstract::Gastrointestinal (GIT) cancers represent the third common cancers worldwide, characterized by rapid progression and higher mortality rate. Matrix metalloproteinases (MMPs) play an important role in cancer metastases. The present study was conducted to estimate and evaluate the role of MMP-7, -9, -10 and -12 and TGF β1...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-020-00651-2
更新日期:2020-11-01 00:00:00
abstract::The incidence of sepsis-associated acute kidney injury (AKI) is on the rise. Recent studies have found a correlation between antithrombin III and AKI. We established a predictive model for sepsis-associated AKI based on plasma ATIII levels. A prospective study (March 2018-January 2020) was conducted in sepsis patients...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-020-00656-x
更新日期:2020-08-31 00:00:00
abstract::The sensitivity of the alkaline comet assay for the evaluation of baseline and treatment-induced DNA damage in white blood cells of breast cancer patients receiving adjuvant chemotherapy according to three conventional anthracycline- and cyclophosphamide-containing protocols was investigated. Additionally, baseline DN...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-006-0113-8
更新日期:2006-12-01 00:00:00
abstract::Longitudinal studies have improved current diagnostics and management of metabolic associated fatty liver disease (MAFLD) patients by liver biopsy and therapeutic intervention, yet the deficiency of biomarker spectrum for dissecting subtypes largely hinders the symptomatic treatment. We originally enriched serum from ...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-020-00636-1
更新日期:2020-11-01 00:00:00
abstract::Vascular endothelial growth factor (VEGF) has been linked with tumor invasion and metastasis. However, the role of VEGF expression in osteosarcoma remains controversial. By searching the PubMed, Embase, and Google Scholar databases, we conducted a meta-analysis to evaluate the pathological and prognostic significance ...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章,meta分析
doi:10.1007/s10238-015-0382-1
更新日期:2016-11-01 00:00:00
abstract::Recently, it was discovered that high mobility group box chromosomal protein 1 (HMGB1) acts as a potent pro-inflammatory cytokine that is released into the extracellular milieu. Signal transducer and activator of transcription (STAT) proteins play an important role in cytokine signaling. Some investigators have report...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-010-0116-3
更新日期:2011-06-01 00:00:00
abstract::Interleukin-17A plays a role in tissue inflammation by inducing release of proinflammatory and neutrophil-mobilizing cytokines. We investigated the association between ulcerative colitis (UC) and polymorphisms of IL17A, rs2275913 (-197 G > A), and rs3748067 (*1249 C > T). The study was performed in 475 healthy subject...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-012-0206-5
更新日期:2013-11-01 00:00:00
abstract::Oxidative stress (OS) has recently been documented in hypothyroidism, a disease more prevalent in women. In general, OS is reported to be more prevalent in males. However, the effect of gender on OS and protein glycation in hypothyroidism has not been addressed. Blood samples from 37 recently diagnosed primary hypothy...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-008-0164-0
更新日期:2008-06-01 00:00:00
abstract::Hyperhomocysteinemia is an independent risk factor for vascular disease, frequently observed in patients with severe renal impairment. Hyperhomocysteinemia has never been considered as a possible risk factor in renal artery stenosis. We investigated plasma folate and vitamin B12, methylenetetrahydrofolate reductase (M...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s102380100005
更新日期:2001-12-01 00:00:00
abstract::Alpha1-adrenoceptor antagonists (alpha1-blockers) are currently used as first-line drugs for the treatment of benign prostatic hyperplasia (BPH). However, cases of BPH are often encountered in which the efficacy of alpha1-blockers decreases and switching to surgical treatment is required. One factor responsible for th...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-009-0073-x
更新日期:2010-06-01 00:00:00
abstract::We retrospectively analyzed the medical history of 19 elderly myeloma patients treated with the "novel subcutaneous formulation of bortezomib." In our experience, two patients (10 %) discontinued treatment for paralytic ileus. The exact pathogenetic mechanisms of toxic megacolon and paralytic ileus due to "novel subcu...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-015-0337-6
更新日期:2016-02-01 00:00:00
abstract::Rho-associated coiled-coil-containing protein kinase 2 (ROCK2) has been known as an effector for the small GTPase Rho and plays an important role in tumor progression and metastasis. However, the effect of ROCK2 in gastric cancer (GC) has not been identified. This study showed that ROCK2 expression significantly incre...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-016-0444-z
更新日期:2017-11-01 00:00:00
abstract::The cross-sectional study was conducted to examine hepatic function via liver enzymes/proteins assessments, along with the estimation of an inflammatory response from C-reactive protein (CRP)-which is a liver-synthesized protein. The liver function tests with aspartate aminotransferase (AST), alanine aminotransferase ...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-017-0462-5
更新日期:2018-02-01 00:00:00
abstract::Anti-inflammatory cytokine and its serological detection may have an important role in the process of cardiovascular and cerebrovascular diseases. We investigated whether serum interleukin-10 (IL-10) is associated with cerebral infarction or not in the general population. Identified comprehensive searching was perform...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章,meta分析
doi:10.1007/s10238-015-0346-5
更新日期:2016-05-01 00:00:00
abstract::Recent findings suggest that cell and gene transplantation in the infarcted myocardium may improve heart function. The aim of the study was to investigate the mechanism involved in improving heart function following the co-injection of adipose-derived stem cells (ADSCs) and basic fibroblast growth factor (bFGF) in a r...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-015-0383-0
更新日期:2016-11-01 00:00:00
abstract::Breast cancer is the most common cause of malignancy and mortality in women worldwide. This study aimed at localising homologous recombination repair (HR) genes and their chromosomal loci and correlating their nucleotide variants with susceptibility to breast cancer. In this study, authors analysed the association bet...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-016-0441-2
更新日期:2017-11-01 00:00:00
abstract::Apoptosis of the acinar and ductal epithelial cells of the salivary glands has been proposed as a mechanism possibly responsible for the impairment of the secretory function in Sjögren's syndrome, an organ-specific autoimmune disorder characterized by destruction of these glandular structures. The presence of serum au...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-009-0087-4
更新日期:2010-09-01 00:00:00
abstract::Severe combined immunodeficiencies (SCID) comprise a spectrum of genetic defects that involve both humoral and cellular immunities. Defects in recombinating activating gene 1 (RAG1), RAG2, Artemis, or LIG4 can disrupt V(D)J recombination. Defective V(D)J recombination of the T and B cell receptors is responsible for T...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-009-0053-1
更新日期:2009-12-01 00:00:00
abstract::Slit2 glycoprotein has been described to regulate the inflammatory response and be involved in autoimmune diseases. Here, we investigated the expression of Slit2 and its potential significance in systemic lupus erythematosus (SLE). A total of 103 patients with SLE participated in our study. The levels of serum Slit2 w...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-020-00664-x
更新日期:2020-10-20 00:00:00
abstract::The aim of this work was to investigate the presence of the RHD gene in fetal cells obtained from amniotic fluid. We studied 65 samples of amniotic fluid, 11 from RhD-negative mothers sensitized with anti-D alloantibodies. The fetal origin of the DNA was confirmed with the analysis of 1 VNTR locus and 3 STR loci in DN...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s102380200010
更新日期:2002-07-01 00:00:00
abstract::Hereditary periodic fever syndromes (HPF) are a group of diseases characterised by recurrences of fever and inflammation separated by symptom-free intervals. Familial Mediterranean fever (FMF) is the most frequent entity within this group of disorders which further consists of hyperimmunoglobulinaemia D and periodic f...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章,评审
doi:10.1007/s10238-005-0071-6
更新日期:2005-10-01 00:00:00
abstract::Mixed-phenotype acute leukemia (MPAL) is the infrequent type of acute leukemia characterized by immunophenotypic and/or cytochemical features of both lineages, but the diagnosis of this disease still is a challenge. In this study, we analyzed immunophenotyping, cytochemistry and frequency of MPAL patients to better di...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-018-0520-7
更新日期:2018-11-01 00:00:00
abstract::Chronic infection with hepatitis C virus (HCV) is a leading cause of liver-related morbidity and mortality throughout the world. Although reliable figures regarding the global prevalence of HCV infection are wanting, it is likely that HCV prevalence will continue to increase. Injection drug use is the most important s...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章,评审
doi:10.1007/s10238-006-0089-4
更新日期:2006-03-01 00:00:00
abstract::Endotoxin is known to cause acute lung injury (ALI). Here, we investigated the effects and mechanisms of recombinant human bone morphogenetic protein-2 (rhBMP-2) on pulmonary arteriole remodeling in endotoxin-induced ALI in rats. Sixty Wistar rats were randomly divided into 3 groups (n = 20). Control group was infused...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-012-0197-2
更新日期:2013-08-01 00:00:00
abstract::B-cell chronic lymphocytic leukemia (B-CLL) is one of the most common leukemias among the elderly and, despite many efforts, still stays incurable. Recent studies point to the microenvironment as the critical factor providing leukemic lymphocytes with pro-survival signals. Thus, the neighboring cells appear to be a pe...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-013-0268-z
更新日期:2015-02-01 00:00:00