Abstract：
:Apoptosis of the acinar and ductal epithelial cells of the salivary glands has been proposed as a mechanism possibly responsible for the impairment of the secretory function in Sjögren's syndrome, an organ-specific autoimmune disorder characterized by destruction of these glandular structures. The presence of serum autoantibodies (Abs) directed against the ribonucleoproteic antigens Ro and La is one of the classification criteria used to identify Sjögren patients, and there is increasing evidence of the direct involvement of Abs in tissue pathogenesis. Our recent report demonstrated that anti-Ro and anti-La Abs are able to trigger the extrinsic pathway of apoptosis in the human salivary gland cells. To better understand how the anti-Ro and anti-La Abs exert their apoptotic effect, human caspase-8 gene expression was examined in primary human salivary gland epithelial cell (SGEC) cultures established from biopsies of labial minor salivary glands. To measure mRNA expression changes of initiating caspase-8, the real-time polymerase chain reaction was employed. This was combined with western blot to study the activation of caspase-8 detecting the cleaved form of caspase-8 and the cleaved poly (ADP-ribose) polymerase, downstream consequences of caspases activation. Data obtained suggest that the anti-Ro and anti-La Abs determine a transcriptional up-regulation and activation of caspase-8. Study of the mRNA in SGEC experimental model may provide insight into the signal transduction pathway stimulated by anti-nuclear autoantibodies.
journal_name
Clin Exp Medjournal_title
Clinical and experimental medicineauthors
Lisi S,Sisto M,Lofrumento D,Frassanito MA,Caprio S,Romano ML,Mitolo V,D'Amore Mdoi
10.1007/s10238-009-0087-4subject
Has Abstractpub_date
2010-09-01 00:00:00pages
199-203issue
3eissn
1591-8890issn
1591-9528journal_volume
10pub_type
杂志文章abstract：:Recently, Th17 cell-associated responses have received growing attention; however, the role of IL-22 (a cytokines also produced by Th17 cells) in the pathogenesis of systemic lupus erythematosus (SLE) has not been widely explored. In this study, we analyze the frequencies of IL-22-positive CD4+/CD8+ T cells in periphe...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-011-0134-9
更新日期：2011-12-01 00:00:00
abstract：:Immunophenotypic characterization of the leukemic cells has been widely used as a tool for diagnosis, classification and prognosis of leukaemia. A total of 192 Chinese patients with acute myeloid leukemia (AML) were immunophenotyped by flow cytometry using a panel of monoclonal antibodies. Among the 192 patients enrol...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-009-0030-8
更新日期：2009-06-01 00:00:00
abstract：:The incidence of sepsis-associated acute kidney injury (AKI) is on the rise. Recent studies have found a correlation between antithrombin III and AKI. We established a predictive model for sepsis-associated AKI based on plasma ATIII levels. A prospective study (March 2018-January 2020) was conducted in sepsis patients...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-020-00656-x
更新日期：2020-08-31 00:00:00
abstract：:Atopic dermatitis (AD) is a chronic, inflammatory skin disease which is characterized by severe pruritus and affects patients' quality of life. In recent years gamma interferon (IFN-gamma) has been accepted as a novel treatment for severe AD, however, its mechanism of action is not clearly identified. Present study ev...
journal_title：Clinical and experimental medicine
pub_type： 临床试验,杂志文章
doi：10.1007/s10238-011-0164-3
更新日期：2012-12-01 00:00:00
abstract：:Imatinib mesylate represents the first of a new generation of molecularly targeted therapies engineered to disrupt signal transduction pathways. It is a tyrosine kinase inhibitor with relatively selective activity against the Abelson (ABL) proto-oncogene, platelet-derived growth factor receptor, and c-KIT receptor. De...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章,评审
doi：10.1007/s10238-004-0032-5
更新日期：2004-09-01 00:00:00
abstract：:The development of new therapies based on tumor biology is one of the main topics in cancer treatment. In this regard, investigating the microenvironment and cellular composition of the tumor is of particular interest. Mesenchymal stem cells (MSCs) are a major group of cells in the tumor tissue and play a critical rol...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章,评审
doi：10.1007/s10238-020-00674-9
更新日期：2021-01-23 00:00:00
abstract：:Anti-tumour necrosis factor-α (TNF-α) therapy brought new hopes for treating rheumatic diseases but also increased the risk of infection, including mycobacterium tuberculosis (MTb). Conventional screening tools, such as tuberculin skin test (TST), lack sensitivity or specificity. Recently, T-SPOT.TB has been introduce...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-010-0123-4
更新日期：2011-09-01 00:00:00
abstract：:Current hepatitis C virus (HCV) genotyping techniques are often highly technical, costly, or need improvements in sensitivity and specificity. These limitations indicate the need of novel methods for HCV genotyping. The present study aimed to develop a novel genotyping method combining high-resolution melting (HRM) an...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-016-0424-3
更新日期：2017-08-01 00:00:00
abstract：:B-cell chronic lymphocytic leukemia (B-CLL) is one of the most common leukemias among the elderly and, despite many efforts, still stays incurable. Recent studies point to the microenvironment as the critical factor providing leukemic lymphocytes with pro-survival signals. Thus, the neighboring cells appear to be a pe...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-013-0268-z
更新日期：2015-02-01 00:00:00
abstract：:To establish a serological classification tree model for rheumatoid arthritis (RA), protein/peptide profiles of serum were detected by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF-MS) combined with weak cationic exchange (WCX) from Cohort 1, including 65 patients with RA and ...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-013-0265-2
更新日期：2015-02-01 00:00:00
abstract：:The predominance of the effector mechanisms by CD4 + T cells is a characteristic of inflammatory autoimmune diseases such as rheumatoid arthritis (RA). The CD40/CD40L costimulatory pathway contributes to these pathogenic mechanisms by promoting autoantibody production and inflammation. Aberrant expression of CD40 and ...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-019-00568-5
更新日期：2019-11-01 00:00:00
abstract：:Cancer stem cells (CSCs) are thought to be a major player in tumor initiation, progression, and metastasis. Targeting CSCs for elimination presents a promising therapeutic strategy; however, this approach will require a stronger understanding of CSC biology and identification of CSC-specific markers. The present study...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-020-00665-w
更新日期：2020-09-23 00:00:00
abstract：:The rat is an established model for studying intestinal adaptations following abdominal surgery. In the study of functional and morphological adaptations of the small intestine, it is helpful to estimate the mucosal surface area. In order to simplify measurements and calculation we developed a new mathematical model f...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s102380200018
更新日期：2002-11-01 00:00:00
abstract：:Slit2 glycoprotein has been described to regulate the inflammatory response and be involved in autoimmune diseases. Here, we investigated the expression of Slit2 and its potential significance in systemic lupus erythematosus (SLE). A total of 103 patients with SLE participated in our study. The levels of serum Slit2 w...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-020-00664-x
更新日期：2020-10-20 00:00:00
abstract：:Primary biliary cirrhosis (PBC) is a progressive autoimmune liver disease in which monocytes/macrophages infiltration and skewed T helper type (Th) 1 and Th17 cell responses participate in the development of the disease. Human peripheral blood monocytes are heterogeneous and can be divided into classical CD14highCD16-...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-015-0381-2
更新日期：2016-11-01 00:00:00
abstract：:Nitric oxide (NO), produced by the nitric oxide synthase (iNOS) enzyme, is the most-important molecule responsible for the killing of Leishmania parasites by macrophages. In previous work we have demonstrated that, after activation with recombinant human interferon-gamma and/or bacterial lipopolysaccharide, human macr...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-001-8025-0
更新日期：2001-09-01 00:00:00
abstract：:The aim of this work was to investigate the presence of the RHD gene in fetal cells obtained from amniotic fluid. We studied 65 samples of amniotic fluid, 11 from RhD-negative mothers sensitized with anti-D alloantibodies. The fetal origin of the DNA was confirmed with the analysis of 1 VNTR locus and 3 STR loci in DN...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s102380200010
更新日期：2002-07-01 00:00:00
abstract：:The aim of this study was to discover potential biomarkers for rheumatoid arthritis (RA) using Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry combined with magnetic beads. Proteomic fingerprint technology combining magnetic beads with MALDI-TOF-MS was used to profile and compare the prote...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-011-0154-5
更新日期：2012-09-01 00:00:00
abstract：:Systemic lupus erythematosus (SLE) is the prototype autoimmune rheumatic disease. The etiology of this disease is incompletely understood; however, environmental factors and genetic predisposition are involved. Cytokine-mediated immunity plays a crucial role in the pathogenesis of SLE. We investigate the association o...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-014-0315-4
更新日期：2015-11-01 00:00:00
abstract：:Chronic infection with hepatitis C virus (HCV) is a leading cause of liver-related morbidity and mortality throughout the world. Although reliable figures regarding the global prevalence of HCV infection are wanting, it is likely that HCV prevalence will continue to increase. Injection drug use is the most important s...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章,评审
doi：10.1007/s10238-006-0089-4
更新日期：2006-03-01 00:00:00
abstract：:The most important mutation associated with Multiple Endocrine Neoplasia type 2B (MEN 2B) is the change of thymine to cytosine in codon 918 of exon 16 in the RET oncogene (ATG → ACG). The aim of this work was to develop a single oligoarray by using tandem hybridization to detect the T918C/RET mutation for MEN 2B patie...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-010-0128-z
更新日期：2011-12-01 00:00:00
abstract：:The objective of this study was to compare between the effects of mesenchymal stem cell (MSC) and betamethasone in the treatment of rheumatoid arthritis. Sixty male albino mice were divided equally into 2 models. They are MSC model, group 1: saline control group, group 2: collagen-induced arthritis (CIA), group 3: ind...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-013-0248-3
更新日期：2014-08-01 00:00:00
abstract：:SOCS3 as an important negative regulator of IL6/JAK/STAT3 signaling pathway may be early critical determinants of carcinogenesis. This study aimed to explore the aberrant promoter methylation of SOCS3 gene in circulating DNA as a noninvasive biomarker for screening hepatocellular carcinoma (HCC) high-risk individuals ...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-017-0473-2
更新日期：2018-02-01 00:00:00
abstract：:Diabetes is one of the most common and challenging health problems. Studies in several nations show that polymorphisms within the transcription factor 7-like 2 genes could be associated with type 2 diabetes (T2D). Therefore, a case-control study was conducted to find the association between SNP rs7903146 and T2D in ou...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-011-0144-7
更新日期：2012-06-01 00:00:00
abstract：:T helper (Th) 17 cells have a critical role in the pathogenesis of chronic hepatitis B virus (HBV) infection, and basic leucine zipper transcription factor, ATF-like (BATF) is a newly identified transcriptional factor regulating the differentiation of Th17 cells. However, its precise role in patients with chronic hepa...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-017-0480-3
更新日期：2018-05-01 00:00:00
abstract：:Type I interferons are currently used for the treatment of chronic viral hepatitis, multiple sclerosis and several hematological and solid tumors. Side effects are not uncommon, and include multiple alterations in thyroid function, some of which are unrelated to autoimmunity. Review of the literature revealed an overa...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章,评审
doi：10.1007/s10238-004-0026-3
更新日期：2004-04-01 00:00:00
abstract：:Alpha1-adrenoceptor antagonists (alpha1-blockers) are currently used as first-line drugs for the treatment of benign prostatic hyperplasia (BPH). However, cases of BPH are often encountered in which the efficacy of alpha1-blockers decreases and switching to surgical treatment is required. One factor responsible for th...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-009-0073-x
更新日期：2010-06-01 00:00:00
abstract：:Nasopharyngeal carcinoma (NPC) is a very common head and neck cancer in southern china. Despite advances in surgical and chemotherapeutic approaches, its prognosis is still not promising. Hedgehog signaling pathway was reported to be involved in a number of cancers including head and neck. However, it remains unclear ...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-012-0198-1
更新日期：2013-11-01 00:00:00
abstract：:Endotoxin is known to cause acute lung injury (ALI). Here, we investigated the effects and mechanisms of recombinant human bone morphogenetic protein-2 (rhBMP-2) on pulmonary arteriole remodeling in endotoxin-induced ALI in rats. Sixty Wistar rats were randomly divided into 3 groups (n = 20). Control group was infused...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-012-0197-2
更新日期：2013-08-01 00:00:00
abstract：:Anti-inflammatory cytokine and its serological detection may have an important role in the process of cardiovascular and cerebrovascular diseases. We investigated whether serum interleukin-10 (IL-10) is associated with cerebral infarction or not in the general population. Identified comprehensive searching was perform...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章,meta分析
doi：10.1007/s10238-015-0346-5
更新日期：2016-05-01 00:00:00
