Abstract：
:Recently, Th17 cell-associated responses have received growing attention; however, the role of IL-22 (a cytokines also produced by Th17 cells) in the pathogenesis of systemic lupus erythematosus (SLE) has not been widely explored. In this study, we analyze the frequencies of IL-22-positive CD4+/CD8+ T cells in peripheral blood mononuclear cells (PBMCs) from patients with SLE and their correlations with disease activity and clinical data. Five-color flow cytometry (FCM) was used to assess IL-22 production of CD4+/CD8+ T cells in PBMCs from 31 patients with SLE and 22 healthy control subjects, following stimulation ex vivo with phorbol 12-myristate 13-acetate and ionomycin for 4 h. Results showed that the percentages of IL-22-positive CD4+ T cells were increased in the PBMCs of patients with SLE compared with healthy control subjects, whereas there were no significant differences in the percentages of IL-22-positive CD8+ T cells. There was a strong positive correlation between the proportion of CD4+ T cells expressing IL-22 and SLEDAI score (r (s) = 0.65, P < 0.001). Furthermore, the frequencies of IL-22-positive CD4+ T cells were significantly higher in patients with SLE with nephritis than those without nephritis (Z = -2.72, P < 0.01). In conclusion, increased frequencies of IL-22-positive CD4+ T cells in patients with SLE and positive correlation with SLEDAI score and lupus nephritis suggest that this cytokine may be implicated in the pathogenesis of the disease.
journal_name
Clin Exp Medjournal_title
Clinical and experimental medicineauthors
Qin WZ,Chen LL,Pan HF,Leng RX,Zhai ZM,Wang C,Li RJ,Wang S,Wang HP,Ye DQdoi
10.1007/s10238-011-0134-9subject
Has Abstractpub_date
2011-12-01 00:00:00pages
245-50issue
4eissn
1591-8890issn
1591-9528journal_volume
11pub_type
杂志文章abstract：:To investigate the expression of cyclin G1, cyclin G2 in nasopharyngeal carcinoma (NPC) cell lines and its significance. The protein expression of cyclin G1, cyclin G2 in NPC cell lines of different differentiation degree (HNE2, CNE1) was detected by indirect immunofluorescence. The mRNA expression of cyclin G1, cycli...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-011-0142-9
更新日期：2012-03-01 00:00:00
abstract：:Mixed-phenotype acute leukemia (MPAL) is the infrequent type of acute leukemia characterized by immunophenotypic and/or cytochemical features of both lineages, but the diagnosis of this disease still is a challenge. In this study, we analyzed immunophenotyping, cytochemistry and frequency of MPAL patients to better di...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-018-0520-7
更新日期：2018-11-01 00:00:00
abstract：:The most important mutation associated with Multiple Endocrine Neoplasia type 2B (MEN 2B) is the change of thymine to cytosine in codon 918 of exon 16 in the RET oncogene (ATG → ACG). The aim of this work was to develop a single oligoarray by using tandem hybridization to detect the T918C/RET mutation for MEN 2B patie...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-010-0128-z
更新日期：2011-12-01 00:00:00
abstract：:Primary biliary cirrhosis (PBC) is a slowly progressive autoimmune disease of unknown mechanism. We established a PBC animal model by injecting C57BL/6 mice with polyinosinic-polycytidylic acid sodium (polyI:C) to investigate the therapeutic effect of bone marrow-derived mesenchymal stem cells (BM-MSC) on this model. ...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-010-0105-6
更新日期：2011-03-01 00:00:00
abstract：:Increased expression of progranulin (PGRN) has been reported in some hematological cancers, but limited information regarding its significance in acute lymphoblastic leukemia (ALL) is available. This study involved 60 subjects (40 de novo adult ALL patients and 20 controls). Serum PGRN level was measured by enzyme-lin...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-020-00610-x
更新日期：2020-05-01 00:00:00
abstract：:Previous studies have implicated protective effects of vitamin D on insulin secretion and pancreas beta cell function. The goal of the present study is to determine if a combination therapy of 25-hydroxyvitamin D3 and insulin had any advantage over insulin therapy alone on lipid peroxidation and antioxidant activity i...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-005-0061-8
更新日期：2005-05-01 00:00:00
abstract：OBJECTIVE:Infection of prosthetic material is a major complication of vascular surgery. Therapy for it includes implantation of antimicrobial prostheses bonded with different antimicrobial agents. These agents may, however, induce an acute phase reaction following implantation in the host, thus compromising follow-up o...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-001-8008-1
更新日期：2001-03-01 00:00:00
abstract：:The sensitivity of the alkaline comet assay for the evaluation of baseline and treatment-induced DNA damage in white blood cells of breast cancer patients receiving adjuvant chemotherapy according to three conventional anthracycline- and cyclophosphamide-containing protocols was investigated. Additionally, baseline DN...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-006-0113-8
更新日期：2006-12-01 00:00:00
abstract：:Immunophenotypic characterization of the leukemic cells has been widely used as a tool for diagnosis, classification and prognosis of leukaemia. A total of 192 Chinese patients with acute myeloid leukemia (AML) were immunophenotyped by flow cytometry using a panel of monoclonal antibodies. Among the 192 patients enrol...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-009-0030-8
更新日期：2009-06-01 00:00:00
abstract：:B-cell chronic lymphocytic leukemia (B-CLL) is one of the most common leukemias among the elderly and, despite many efforts, still stays incurable. Recent studies point to the microenvironment as the critical factor providing leukemic lymphocytes with pro-survival signals. Thus, the neighboring cells appear to be a pe...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-013-0268-z
更新日期：2015-02-01 00:00:00
abstract：:Anti-inflammatory cytokine and its serological detection may have an important role in the process of cardiovascular and cerebrovascular diseases. We investigated whether serum interleukin-10 (IL-10) is associated with cerebral infarction or not in the general population. Identified comprehensive searching was perform...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章,meta分析
doi：10.1007/s10238-015-0346-5
更新日期：2016-05-01 00:00:00
abstract：:Oxidative stress (OS) has recently been documented in hypothyroidism, a disease more prevalent in women. In general, OS is reported to be more prevalent in males. However, the effect of gender on OS and protein glycation in hypothyroidism has not been addressed. Blood samples from 37 recently diagnosed primary hypothy...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-008-0164-0
更新日期：2008-06-01 00:00:00
abstract：:STAT6 transcription factor, which has been implicated in commitment to Th2, is known to be activated by IL-4 and IL-13. Accordingly, STAT6 is primarily responsible for the transcriptional effects of IL-4 and IL-13. STAT6-deficient mice are known to have defective IL-4-mediated functions, such as B cell proliferation, ...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-009-0083-8
更新日期：2010-09-01 00:00:00
abstract：:Current hepatitis C virus (HCV) genotyping techniques are often highly technical, costly, or need improvements in sensitivity and specificity. These limitations indicate the need of novel methods for HCV genotyping. The present study aimed to develop a novel genotyping method combining high-resolution melting (HRM) an...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-016-0424-3
更新日期：2017-08-01 00:00:00
abstract：:Macrophage migration inhibitory factor (MIF) has been reported to associate with increased cancer risk in several cancers. However, the role of MIF in breast cancer (BC) susceptibility remains unknown. For the first time, we conducted a case-control study to assess the potential association of three common MIF gene va...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-016-0439-9
更新日期：2017-08-01 00:00:00
abstract：:It is suggested that gastric mucins, and in particular some specific glycan structures that can act as carbohydrate receptors, are involved in the interactions with Helicobacter pylori adhesins. The main aim of our study was to evaluate glycosylation pattern of glycoproteins of gastric juice before and at the end of e...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-010-0113-6
更新日期：2011-06-01 00:00:00
abstract：:Longitudinal studies have improved current diagnostics and management of metabolic associated fatty liver disease (MAFLD) patients by liver biopsy and therapeutic intervention, yet the deficiency of biomarker spectrum for dissecting subtypes largely hinders the symptomatic treatment. We originally enriched serum from ...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-020-00636-1
更新日期：2020-11-01 00:00:00
abstract：:Many tumor cells express chemokines and chemokine receptors, and these molecules can affect both tumor progression and anti-tumor immune response. Genetic polymorphisms of some chemokine receptors were found to be closely related to malignant tumors, especially in metastasis process, including breast cancer (BC). Cons...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章,评审
doi：10.1007/s10238-015-0342-9
更新日期：2016-05-01 00:00:00
abstract：:Twist1 is a key transcription factor, which confers tumor cells with cancer stem cell (CSC)-like characteristics and enhances epithelial-mesenchymal transition in pathological conditions including tumor malignancy and metastasis. This study aimed to evaluate the expression patterns and clinical significance of Twist1 ...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-017-0481-2
更新日期：2018-05-01 00:00:00
abstract：:The aim of this work was to investigate the presence of the RHD gene in fetal cells obtained from amniotic fluid. We studied 65 samples of amniotic fluid, 11 from RhD-negative mothers sensitized with anti-D alloantibodies. The fetal origin of the DNA was confirmed with the analysis of 1 VNTR locus and 3 STR loci in DN...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s102380200010
更新日期：2002-07-01 00:00:00
abstract：:Imatinib mesylate represents the first of a new generation of molecularly targeted therapies engineered to disrupt signal transduction pathways. It is a tyrosine kinase inhibitor with relatively selective activity against the Abelson (ABL) proto-oncogene, platelet-derived growth factor receptor, and c-KIT receptor. De...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章,评审
doi：10.1007/s10238-004-0032-5
更新日期：2004-09-01 00:00:00
abstract：:Atopic dermatitis (AD) is a chronic, inflammatory skin disease which is characterized by severe pruritus and affects patients' quality of life. In recent years gamma interferon (IFN-gamma) has been accepted as a novel treatment for severe AD, however, its mechanism of action is not clearly identified. Present study ev...
journal_title：Clinical and experimental medicine
pub_type： 临床试验,杂志文章
doi：10.1007/s10238-011-0164-3
更新日期：2012-12-01 00:00:00
abstract：:The aim of this study was to discover potential biomarkers for rheumatoid arthritis (RA) using Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry combined with magnetic beads. Proteomic fingerprint technology combining magnetic beads with MALDI-TOF-MS was used to profile and compare the prote...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-011-0154-5
更新日期：2012-09-01 00:00:00
abstract：:Behcet's disease is a recurrent multisystem vasculitis that can affect any organ or system, but was originally described as orogenital ulcerations and uveitis. Specific criteria have been proposed for diagnosis and identification of affected organs by different national authorities. Behcet's disease is proposed to be ...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章,评审
doi：10.1007/s10238-004-0045-0
更新日期：2004-12-01 00:00:00
abstract：:Arginase activity was measured in serum and biopsy from healthy individuals and colorectal cancer patients. Arginase activity in tumor samples (87 +/- 7.7 U/g tissue) was significantly higher than in controls (40.7 +/- 3.3 U/g tissue). However, serum arginase activity did not show any significant change in both groups...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s102380200007
更新日期：2002-05-01 00:00:00
abstract：:The development of new therapies based on tumor biology is one of the main topics in cancer treatment. In this regard, investigating the microenvironment and cellular composition of the tumor is of particular interest. Mesenchymal stem cells (MSCs) are a major group of cells in the tumor tissue and play a critical rol...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章,评审
doi：10.1007/s10238-020-00674-9
更新日期：2021-01-23 00:00:00
abstract：:The aim of this study was to evaluate the ferric-reducing ability of serum (FRAS), paraoxonase 1 (PON1), ceruloplasmin serum oxidase activity and hsCRP level in patients with type1 diabetes mellitus without and with diabetic retinopathy. The study was performed in 76 patients with type 1 diabetes mellitus, 35 without ...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-009-0084-7
更新日期：2010-09-01 00:00:00
abstract：:The rat is an established model for studying intestinal adaptations following abdominal surgery. In the study of functional and morphological adaptations of the small intestine, it is helpful to estimate the mucosal surface area. In order to simplify measurements and calculation we developed a new mathematical model f...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s102380200018
更新日期：2002-11-01 00:00:00
abstract：:Vascular endothelial growth factor (VEGF) has been linked with tumor invasion and metastasis. However, the role of VEGF expression in osteosarcoma remains controversial. By searching the PubMed, Embase, and Google Scholar databases, we conducted a meta-analysis to evaluate the pathological and prognostic significance ...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章,meta分析
doi：10.1007/s10238-015-0382-1
更新日期：2016-11-01 00:00:00
abstract：:The effect of preimmunisation with cancer procoagulant (CP) on the growth of Walker 256 carcinosarcoma cells in Wistar Lew/Han/IMP rats in vivo has been analysed. One group of rats was immunised with CP purified from human amnion-chorion membranes. The rest of the animals (control groups) were injected with 0.9% NaCl ...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-006-0106-7
更新日期：2006-10-01 00:00:00
