Abstract:
:Nitric oxide (NO), produced by the nitric oxide synthase (iNOS) enzyme, is the most-important molecule responsible for the killing of Leishmania parasites by macrophages. In previous work we have demonstrated that, after activation with recombinant human interferon-gamma and/or bacterial lipopolysaccharide, human macrophages infected with Leishmania infantum are able to produce nitric oxide and to express nitric oxide synthase. The arachidonate derivative prostaglandin E2 has been shown to modulate various macrophage activities, and in particular nitric oxide production, sometimes with opposite effects, related to experimental conditions. In this work we have evaluated nitric oxide release and parasite killing by peripheral blood-derived L. infantum-infected human macrophages in vitro stimulated with lipopolysaccharide and simultaneously treated with prostaglandin E2. Experiments were also performed in the presence of the nitric oxide synthase inhibitor L-NGmonomethylarginine (L-NMMA) and of the cyclooxygenase inhibitor indomethacin. Nitric oxide release in supernatants of macrophage cultures was measured by the Griess reaction for nitrites. Parasite killing was microscopically evaluated by fluorescent dyes. Results demonstrated that macrophages stimulated with lipopolysaccharide and treated with prostaglandin E2 exhibited increased nitric oxide production and parasite killing, which were significantly reduced by either L-NMMA or indomethacin. In indomethacin-treated macrophages, nitric oxide production and leishmanicidal ability were partially restored by the addition of exogenous prostaglandin E2. Taken together, these results indicate that prostaglandin E2 may be involved in nitric oxide production, and possibly in the host-protective immune response against Leishmania. Moreover, the demonstration of a stimulatory role of prostaglandin E2 on nitric oxide production induced by intracellular pathogens in humans is interesting in the light of a possible pharmacological regulation of nitric oxide by modulation of prostaglandin E2 synthesis.
journal_name
Clin Exp Medjournal_title
Clinical and experimental medicineauthors
Panaro MA,Brandonisio O,Sisto M,Acquafredda A,Leogrande D,Fumarola L,Mitolo Vdoi
10.1007/s10238-001-8025-0subject
Has Abstractpub_date
2001-09-01 00:00:00pages
137-43issue
3eissn
1591-8890issn
1591-9528journal_volume
1pub_type
杂志文章abstract::Anti-inflammatory cytokine and its serological detection may have an important role in the process of cardiovascular and cerebrovascular diseases. We investigated whether serum interleukin-10 (IL-10) is associated with cerebral infarction or not in the general population. Identified comprehensive searching was perform...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章,meta分析
doi:10.1007/s10238-015-0346-5
更新日期:2016-05-01 00:00:00
abstract::Interleukin (IL)-27 is an IL-12 family cytokine and exerts a critical role in immune regulation in the context of infection, autoimmunity, and angiogenesis. In this study, we aimed to investigate the possible pathophysiological role of IL-27 and vascular endothelial growth factor (VEGF) in ankylosing spondylitis (AS)....
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-014-0281-x
更新日期:2015-05-01 00:00:00
abstract::Pancreatic islet transplantation has remained an effective therapy for type 1 diabetes since 2000. Its widespread use has been prohibited by the shortage of suitable donors. It is critical to explore an applicable alternative for β-cell replacement. This study was performed to generate insulin-producing cells (IPCs) f...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-014-0319-0
更新日期:2015-11-01 00:00:00
abstract::Triglyceride-rich lipoproteins contain both apolipoproteins E (ApoE) and C-III (ApoC-III), which show opposite functional properties. The relationships between the ApoE (epsilon2/epsilon3/epsilon4) gene polymorphism and ApoC-III/ApoE ratio has never been investigated. A large population (n=552) of cardiovascular patie...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-007-0142-y
更新日期:2007-12-01 00:00:00
abstract::The melphalan-prednisone regimen has been considered as standard therapy for patients with multiple myeloma (MM) for many years. Recently, high-dose chemotherapy with stem-cell support has extended progression-free survival and increased overall survival, and it is now considered conventional therapy in younger patien...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章,评审
doi:10.1007/s10238-007-0134-y
更新日期:2007-09-01 00:00:00
abstract::The aim of this study was to evaluate the ferric-reducing ability of serum (FRAS), paraoxonase 1 (PON1), ceruloplasmin serum oxidase activity and hsCRP level in patients with type1 diabetes mellitus without and with diabetic retinopathy. The study was performed in 76 patients with type 1 diabetes mellitus, 35 without ...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-009-0084-7
更新日期:2010-09-01 00:00:00
abstract::Longitudinal studies have improved current diagnostics and management of metabolic associated fatty liver disease (MAFLD) patients by liver biopsy and therapeutic intervention, yet the deficiency of biomarker spectrum for dissecting subtypes largely hinders the symptomatic treatment. We originally enriched serum from ...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-020-00636-1
更新日期:2020-11-01 00:00:00
abstract::Telomere shortening occurs with human aging in many organs and tissues and is accelerated by rapid cell turnover and oxidative injury. To clarify the clinical importance of telomere shortening in colonic mucosa in ulcerative colitis (UC), we measured average telomere length using quantitative real-time PCR in non-neop...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-014-0295-4
更新日期:2015-08-01 00:00:00
abstract::The sensitivity of the alkaline comet assay for the evaluation of baseline and treatment-induced DNA damage in white blood cells of breast cancer patients receiving adjuvant chemotherapy according to three conventional anthracycline- and cyclophosphamide-containing protocols was investigated. Additionally, baseline DN...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-006-0113-8
更新日期:2006-12-01 00:00:00
abstract::Twist1 is a key transcription factor, which confers tumor cells with cancer stem cell (CSC)-like characteristics and enhances epithelial-mesenchymal transition in pathological conditions including tumor malignancy and metastasis. This study aimed to evaluate the expression patterns and clinical significance of Twist1 ...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-017-0481-2
更新日期:2018-05-01 00:00:00
abstract::Current hepatitis C virus (HCV) genotyping techniques are often highly technical, costly, or need improvements in sensitivity and specificity. These limitations indicate the need of novel methods for HCV genotyping. The present study aimed to develop a novel genotyping method combining high-resolution melting (HRM) an...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-016-0424-3
更新日期:2017-08-01 00:00:00
abstract::The identification of chromosome 1 translocations and deletions is a rare and poorly investigated event in chronic lymphocytic leukemia (CLL). Nevertheless, the identification of novel additional molecular alterations is of great interest, opening to new prognostic and therapeutic strategies for such heterogeneous hem...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-019-00551-0
更新日期:2019-05-01 00:00:00
abstract::Increased expression of progranulin (PGRN) has been reported in some hematological cancers, but limited information regarding its significance in acute lymphoblastic leukemia (ALL) is available. This study involved 60 subjects (40 de novo adult ALL patients and 20 controls). Serum PGRN level was measured by enzyme-lin...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-020-00610-x
更新日期:2020-05-01 00:00:00
abstract::Type I interferons are currently used for the treatment of chronic viral hepatitis, multiple sclerosis and several hematological and solid tumors. Side effects are not uncommon, and include multiple alterations in thyroid function, some of which are unrelated to autoimmunity. Review of the literature revealed an overa...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章,评审
doi:10.1007/s10238-004-0026-3
更新日期:2004-04-01 00:00:00
abstract::The effect of preimmunisation with cancer procoagulant (CP) on the growth of Walker 256 carcinosarcoma cells in Wistar Lew/Han/IMP rats in vivo has been analysed. One group of rats was immunised with CP purified from human amnion-chorion membranes. The rest of the animals (control groups) were injected with 0.9% NaCl ...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-006-0106-7
更新日期:2006-10-01 00:00:00
abstract::Macrophage migration inhibitory factor (MIF) has been reported to associate with increased cancer risk in several cancers. However, the role of MIF in breast cancer (BC) susceptibility remains unknown. For the first time, we conducted a case-control study to assess the potential association of three common MIF gene va...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-016-0439-9
更新日期:2017-08-01 00:00:00
abstract::The present study was initiated to examine whether the concentration of CO in the breath is elevated in patients with inflammatory bowel disease (IBD). Twenty-three clinically stable patients with IBD in the outpatient clinic (11 with Crohn's disease, 12 with ulcerative colitis), who are non-smokers and non-passive sm...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-007-0129-8
更新日期:2007-06-01 00:00:00
abstract::Pancreatic cancer (PC) is a leading cause of cancer death worldwide, especially in Western societies. Its aggressive nature and poor prognosis increase the need for identifying new and more accurate diagnostic and prognostic tools. We studied 41 patients who had undergone radical surgical resection for PC, investigate...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-015-0352-7
更新日期:2016-08-01 00:00:00
abstract::Interleukin-17A plays a role in tissue inflammation by inducing release of proinflammatory and neutrophil-mobilizing cytokines. We investigated the association between ulcerative colitis (UC) and polymorphisms of IL17A, rs2275913 (-197 G > A), and rs3748067 (*1249 C > T). The study was performed in 475 healthy subject...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-012-0206-5
更新日期:2013-11-01 00:00:00
abstract::Recently, it was discovered that high mobility group box chromosomal protein 1 (HMGB1) acts as a potent pro-inflammatory cytokine that is released into the extracellular milieu. Signal transducer and activator of transcription (STAT) proteins play an important role in cytokine signaling. Some investigators have report...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-010-0116-3
更新日期:2011-06-01 00:00:00
abstract::An in vitro skin explant model was originally developed to predict the occurrence and severity of acute graft-versus-host disease in allogeneic hematopoietic stem cell transplants. In previous studies we reported that peripheral blood mononuclear cells of patients with rheumatoid arthritis were able to induce graft-ve...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-001-8014-3
更新日期:2001-06-01 00:00:00
abstract::Recent findings suggest that cell and gene transplantation in the infarcted myocardium may improve heart function. The aim of the study was to investigate the mechanism involved in improving heart function following the co-injection of adipose-derived stem cells (ADSCs) and basic fibroblast growth factor (bFGF) in a r...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-015-0383-0
更新日期:2016-11-01 00:00:00
abstract::SOCS3 as an important negative regulator of IL6/JAK/STAT3 signaling pathway may be early critical determinants of carcinogenesis. This study aimed to explore the aberrant promoter methylation of SOCS3 gene in circulating DNA as a noninvasive biomarker for screening hepatocellular carcinoma (HCC) high-risk individuals ...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-017-0473-2
更新日期:2018-02-01 00:00:00
abstract::Cancer stem cells (CSCs) are thought to be a major player in tumor initiation, progression, and metastasis. Targeting CSCs for elimination presents a promising therapeutic strategy; however, this approach will require a stronger understanding of CSC biology and identification of CSC-specific markers. The present study...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-020-00665-w
更新日期:2020-09-23 00:00:00
abstract::It is suggested that gastric mucins, and in particular some specific glycan structures that can act as carbohydrate receptors, are involved in the interactions with Helicobacter pylori adhesins. The main aim of our study was to evaluate glycosylation pattern of glycoproteins of gastric juice before and at the end of e...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-010-0113-6
更新日期:2011-06-01 00:00:00
abstract::The purpose of this study was to reveal the clinical diagnostic and prognostic value of miR-768-3p in HBV-related HCC and to investigate its effect on the biological function of HCC. Quantitative real-time polymerase chain reaction was used to detect the expression level of miR-768-3p in subjects' serum. The receiver ...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-020-00646-z
更新日期:2020-11-01 00:00:00
abstract::The incidence of sepsis-associated acute kidney injury (AKI) is on the rise. Recent studies have found a correlation between antithrombin III and AKI. We established a predictive model for sepsis-associated AKI based on plasma ATIII levels. A prospective study (March 2018-January 2020) was conducted in sepsis patients...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-020-00656-x
更新日期:2020-08-31 00:00:00
abstract::T helper (Th) 17 cells have a critical role in the pathogenesis of chronic hepatitis B virus (HBV) infection, and basic leucine zipper transcription factor, ATF-like (BATF) is a newly identified transcriptional factor regulating the differentiation of Th17 cells. However, its precise role in patients with chronic hepa...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s10238-017-0480-3
更新日期:2018-05-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) have captured the attention of researchers today due to their multipotent differentiation capacity. Also, they have been successfully applied clinically, in the treatment of various diseases of the heart and musculoskeletal systems, with encouraging results. Their supportive role in haema...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章,评审
doi:10.1007/s10238-013-0247-4
更新日期:2014-08-01 00:00:00
abstract::The aim of this work was to investigate the presence of the RHD gene in fetal cells obtained from amniotic fluid. We studied 65 samples of amniotic fluid, 11 from RhD-negative mothers sensitized with anti-D alloantibodies. The fetal origin of the DNA was confirmed with the analysis of 1 VNTR locus and 3 STR loci in DN...
journal_title:Clinical and experimental medicine
pub_type: 杂志文章
doi:10.1007/s102380200010
更新日期:2002-07-01 00:00:00