当前位置： SCI文献检索 > CLINICAL AND EXPERIMENTAL MEDICINE期刊下所有文献 > Single oligoarray-based detection of specific M918T mutation in RET oncogene in multiple endocrine neoplasia type 2B.

Single oligoarray-based detection of specific M918T mutation in RET oncogene in multiple endocrine neoplasia type 2B.

Abstract：

:The most important mutation associated with Multiple Endocrine Neoplasia type 2B (MEN 2B) is the change of thymine to cytosine in codon 918 of exon 16 in the RET oncogene (ATG → ACG). The aim of this work was to develop a single oligoarray by using tandem hybridization to detect the T918C/RET mutation for MEN 2B patients. Two genetically non-related families were studied; each family had a member affected by MEN2B. Both patients presented the T918C/RET mutation in a heterozygous fashion. None of the relatives was positive for this mutation; thus, these cases arose de novo. The proper mutation was confirmed by with different tools, PCR-Fok I endonuclease, direct sequencing, and also using our oligoarray. In this case, it is suitable to use a DNA target smaller than 150 bases with single- or double-stranded DNA and short probes of 7-mer. It was also possible to detect the mutation by employing different sources of DNA, fresh or paraffin-embedded tissues. Therefore, the present oligoarray can identify the most common M918T mutation of RET oncogene from a variety of DNA sources with good specificity and be a good alternative in the molecular diagnosis for MEN 2B cases.

journal_name

Clin Exp Med

journal_title

Clinical and experimental medicine

authors

Pacheco-Rivera RA,Hernández-Zamora E,González-Yebra B,Beattie K,Maldonado-Rodríguez R,Santiago-Hernández JC,Medrano-Ortiz de Zárate ME,Salcedo M

doi

10.1007/s10238-010-0128-z

subject

Has Abstract

pub_date

2011-12-01 00:00:00

pages

227-34

issue

4

eissn

1591-8890

issn

1591-9528

journal_volume

11

pub_type

杂志文章

相关文献

CLINICAL AND EXPERIMENTAL MEDICINE文献大全
  • LncPRYP4-3 serves as a novel diagnostic biomarker for dissecting subtypes of metabolic associated fatty liver disease by targeting RPS4Y2.

    abstract：:Longitudinal studies have improved current diagnostics and management of metabolic associated fatty liver disease (MAFLD) patients by liver biopsy and therapeutic intervention, yet the deficiency of biomarker spectrum for dissecting subtypes largely hinders the symptomatic treatment. We originally enriched serum from ...

    journal_title：Clinical and experimental medicine

    pub_type： 杂志文章

    doi：10.1007/s10238-020-00636-1

    authors： Yang H,Li Q,Zhang L,Zhu M,Niu J,Xue F,Yang L,Qu Q,Lao Y,Ding Z,Xiao C,Wang K

    更新日期：2020-11-01 00:00:00

  • Metabolic syndrome and chronic arthritis: effects of anti-TNF-α therapy.

    abstract：:TNF-α plays a key role in the inflammatory cytokine cascade involved in the pathogenesis of chronic arthritis, including rheumatoid arthritis, psoriatic arthritis, and ankylosing spondylitis. Systemic inflammation and the increased production of pro-inflammatory cytokines in these patients may favor the onset of metab...

    journal_title：Clinical and experimental medicine

    pub_type： 杂志文章,评审

    doi：10.1007/s10238-014-0323-4

    authors： Maruotti N,d'Onofrio F,Cantatore FP

    更新日期：2015-11-01 00:00:00

  • ApoE epsilon2/epsilon3/epsilon4 polymorphism, ApoC-III/ApoE ratio and metabolic syndrome.

    abstract：:Triglyceride-rich lipoproteins contain both apolipoproteins E (ApoE) and C-III (ApoC-III), which show opposite functional properties. The relationships between the ApoE (epsilon2/epsilon3/epsilon4) gene polymorphism and ApoC-III/ApoE ratio has never been investigated. A large population (n=552) of cardiovascular patie...

    journal_title：Clinical and experimental medicine

    pub_type： 杂志文章

    doi：10.1007/s10238-007-0142-y

    authors： Olivieri O,Martinelli N,Bassi A,Trabetti E,Girelli D,Pizzolo F,Friso S,Pignatti PF,Corrocher R

    更新日期：2007-12-01 00:00:00

  • The oxidant and antioxidant effects of 25-hydroxyvitamin D3 in liver, kidney and heart tissues of diabetic rats.

    abstract：:Previous studies have implicated protective effects of vitamin D on insulin secretion and pancreas beta cell function. The goal of the present study is to determine if a combination therapy of 25-hydroxyvitamin D3 and insulin had any advantage over insulin therapy alone on lipid peroxidation and antioxidant activity i...

    journal_title：Clinical and experimental medicine

    pub_type： 杂志文章

    doi：10.1007/s10238-005-0061-8

    authors： Noyan T,Balaharoğlu R,Kömüroğlu U

    更新日期：2005-05-01 00:00:00

  • Anti-cytomegalovirus (CMV) IgG antibody titer in patients with risk factors to atherosclerosis.

    abstract：:Studies have demonstrated cytomegalovirus (CMV) DNA particles in restenotic lesions in atherosclerotic coronary arteries. We have shown that high (>1:800) anti-CMV IgG antibody titers in the serum are associated with active coronary disease and with post coronary angioplasty restenosis. In this study we assessed the a...

    journal_title：Clinical and experimental medicine

    pub_type： 杂志文章

    doi：10.1007/s10238-003-0019-7

    authors： Blum A,Peleg A,Weinberg M

    更新日期：2003-11-01 00:00:00

  • Prognostic significance of serum progranulin level in de novo adult acute lymphoblastic leukemia patients.

    abstract：:Increased expression of progranulin (PGRN) has been reported in some hematological cancers, but limited information regarding its significance in acute lymphoblastic leukemia (ALL) is available. This study involved 60 subjects (40 de novo adult ALL patients and 20 controls). Serum PGRN level was measured by enzyme-lin...

    journal_title：Clinical and experimental medicine

    pub_type： 杂志文章

    doi：10.1007/s10238-020-00610-x

    authors： El-Ghammaz AMS,Azzazi MO,Mostafa N,Hegab HM,Mahmoud AA

    更新日期：2020-05-01 00:00:00

  • Glycosylation of mucins present in gastric juice: the effect of helicobacter pylori eradication treatment.

    abstract：:It is suggested that gastric mucins, and in particular some specific glycan structures that can act as carbohydrate receptors, are involved in the interactions with Helicobacter pylori adhesins. The main aim of our study was to evaluate glycosylation pattern of glycoproteins of gastric juice before and at the end of e...

    journal_title：Clinical and experimental medicine

    pub_type： 杂志文章

    doi：10.1007/s10238-010-0113-6

    authors： Radziejewska I,Borzym-Kluczyk M,Namiot Z,Stefańska E

    更新日期：2011-06-01 00:00:00

  • Telomere length in non-neoplastic colonic mucosa in ulcerative colitis (UC) and its relationship to the severe clinical phenotypes.

    abstract：:Telomere shortening occurs with human aging in many organs and tissues and is accelerated by rapid cell turnover and oxidative injury. To clarify the clinical importance of telomere shortening in colonic mucosa in ulcerative colitis (UC), we measured average telomere length using quantitative real-time PCR in non-neop...

    journal_title：Clinical and experimental medicine

    pub_type： 杂志文章

    doi：10.1007/s10238-014-0295-4

    authors： Tahara T,Shibata T,Okubo M,Kawamura T,Sumi K,Ishizuka T,Nakamura M,Nagasaka M,Nakagawa Y,Ohmiya N,Arisawa T,Hirata I

    更新日期：2015-08-01 00:00:00

  • Slit2 is a potential biomarker for renal impairment in systemic lupus erythematosus.

    abstract：:Slit2 glycoprotein has been described to regulate the inflammatory response and be involved in autoimmune diseases. Here, we investigated the expression of Slit2 and its potential significance in systemic lupus erythematosus (SLE). A total of 103 patients with SLE participated in our study. The levels of serum Slit2 w...

    journal_title：Clinical and experimental medicine

    pub_type： 杂志文章

    doi：10.1007/s10238-020-00664-x

    authors： Zhang Y,Hu L,Li X,Chen L,Yang X

    更新日期：2020-10-20 00:00:00

  • Lenalidomide potentiates CD4+CD25+Treg-related suppression of lymphoma B-cell proliferation.

    abstract：:We have previously found that ex vivo expanded human CD4+CD25+Treg cells suppress proliferation of lymphoma B-cell lines. Here we demonstrate that the immunomodulatory drug lenalidomide potentiates suppression of lymphoma B-cell proliferation by freshly isolated CD4+CD25+Tregs, as well as suppression by Tregs expanded...

    journal_title：Clinical and experimental medicine

    pub_type： 杂志文章

    doi：10.1007/s10238-016-0411-8

    authors： Grygorowicz MA,Borycka IS,Nowak E,Paszkiewicz-Kozik E,Rymkiewicz G,Błachnio K,Biernacka M,Bujko M,Walewski J,Markowicz S

    更新日期：2017-05-01 00:00:00

  • Apolipoprotein E polymorphism is not associated with lipid levels and coronary artery disease in Greek patients with familial hypercholesterolaemia.

    abstract：:Familial hypercholesterolaemia is a genetic disorder characterised by high low-density lipoprotein (LDL) cholesterol concentrations, which frequently gives rise to premature coronary artery disease (CAD). The clinical expression of familial hypercholesterolaemia is highly variable even in patients carrying the same LD...

    journal_title：Clinical and experimental medicine

    pub_type： 杂志文章

    doi：10.1007/s10238-005-0086-z

    authors： Pitsavos C,Choumerianou DM,Skoumas J,Maumus S,Stefanadis C,Dedoussis GV,Visvikis-Siest S

    更新日期：2005-12-01 00:00:00

  • A prediction model of sepsis-associated acute kidney injury based on antithrombin III.

    abstract：:The incidence of sepsis-associated acute kidney injury (AKI) is on the rise. Recent studies have found a correlation between antithrombin III and AKI. We established a predictive model for sepsis-associated AKI based on plasma ATIII levels. A prospective study (March 2018-January 2020) was conducted in sepsis patients...

    journal_title：Clinical and experimental medicine

    pub_type： 杂志文章

    doi：10.1007/s10238-020-00656-x

    authors： Xie Y,Zhang Y,Tian R,Jin W,Du J,Zhou Z,Wang R

    更新日期：2020-08-31 00:00:00

  • Establishing serological classification tree model in rheumatoid arthritis using combination of MALDI-TOF-MS and magnetic beads.

    abstract：:To establish a serological classification tree model for rheumatoid arthritis (RA), protein/peptide profiles of serum were detected by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF-MS) combined with weak cationic exchange (WCX) from Cohort 1, including 65 patients with RA and ...

    journal_title：Clinical and experimental medicine

    pub_type： 杂志文章

    doi：10.1007/s10238-013-0265-2

    authors： Yan Z,Chaojun H,Chuiwen D,Xiaomei L,Xin Z,Yongzhe L,Fengchun Z

    更新日期：2015-02-01 00:00:00

  • FDG PET/CT as a survival prognostic factor in patients with advanced renal cell carcinoma.

    abstract：:Accurate prediction of the outcome of molecular target-based treatment in advanced renal cell carcinoma (RCC) is an important clinical problem. Positron emission tomography/computed tomography using [18F]-2-fluoro-2-deoxyglucose (FDG PET/CT) is a noninvasive tool for the assessment of glucose accumulation which can be...

    journal_title：Clinical and experimental medicine

    pub_type： 杂志文章

    doi：10.1007/s10238-018-0539-9

    authors： Pankowska V,Malkowski B,Wedrowski M,Wedrowska E,Roszkowski K

    更新日期：2019-02-01 00:00:00

  • Elevated circulating CD14lowCD16+ monocyte subset in primary biliary cirrhosis correlates with liver injury and promotes Th1 polarization.

    abstract：:Primary biliary cirrhosis (PBC) is a progressive autoimmune liver disease in which monocytes/macrophages infiltration and skewed T helper type (Th) 1 and Th17 cell responses participate in the development of the disease. Human peripheral blood monocytes are heterogeneous and can be divided into classical CD14highCD16-...

    journal_title：Clinical and experimental medicine

    pub_type： 杂志文章

    doi：10.1007/s10238-015-0381-2

    authors： Peng A,Ke P,Zhao R,Lu X,Zhang C,Huang X,Tian G,Huang J,Wang J,Invernizzi P,Chen Q,Zhuang J

    更新日期：2016-11-01 00:00:00

  • Upregulation of ROCK2 in gastric cancer cell promotes tumor cell proliferation, metastasis and invasion.

    abstract：:Rho-associated coiled-coil-containing protein kinase 2 (ROCK2) has been known as an effector for the small GTPase Rho and plays an important role in tumor progression and metastasis. However, the effect of ROCK2 in gastric cancer (GC) has not been identified. This study showed that ROCK2 expression significantly incre...

    journal_title：Clinical and experimental medicine

    pub_type： 杂志文章

    doi：10.1007/s10238-016-0444-z

    authors： Li M,Ke J,Wang Q,Qian H,Yang L,Zhang X,Xiao J,Ding H,Shan X,Liu Q,Xiao Y,Bao B,Huang H

    更新日期：2017-11-01 00:00:00

  • Paralytic ileus following "subcutaneous bortezomib" therapy: focus on the clinical emergency-report of two cases.

    abstract：:We retrospectively analyzed the medical history of 19 elderly myeloma patients treated with the "novel subcutaneous formulation of bortezomib." In our experience, two patients (10 %) discontinued treatment for paralytic ileus. The exact pathogenetic mechanisms of toxic megacolon and paralytic ileus due to "novel subcu...

    journal_title：Clinical and experimental medicine

    pub_type： 杂志文章

    doi：10.1007/s10238-015-0337-6

    authors： Mele G,Coppi MR,Melpignano A,Quarta G

    更新日期：2016-02-01 00:00:00

  • Mixed-phenotype acute leukemia characteristics: first report from Iran.

    abstract：:Mixed-phenotype acute leukemia (MPAL) is the infrequent type of acute leukemia characterized by immunophenotypic and/or cytochemical features of both lineages, but the diagnosis of this disease still is a challenge. In this study, we analyzed immunophenotyping, cytochemistry and frequency of MPAL patients to better di...

    journal_title：Clinical and experimental medicine

    pub_type： 杂志文章

    doi：10.1007/s10238-018-0520-7

    authors： Poopak B,Khosravi A,Bahoush-Mehdiabadi G,Madani T,Khodadi E,Farahani Z,Vahedi AA,Khosravipour G,Poopak P,Poopak AH

    更新日期：2018-11-01 00:00:00

  • Tumor-resident adenosine-producing mesenchymal stem cells as a potential target for cancer treatment.

    abstract：:The development of new therapies based on tumor biology is one of the main topics in cancer treatment. In this regard, investigating the microenvironment and cellular composition of the tumor is of particular interest. Mesenchymal stem cells (MSCs) are a major group of cells in the tumor tissue and play a critical rol...

    journal_title：Clinical and experimental medicine

    pub_type： 杂志文章,评审

    doi：10.1007/s10238-020-00674-9

    authors： Arab S,Alizadeh A,Asgharzade S

    更新日期：2021-01-23 00:00:00

  • BAL biomarkers' panel for differential diagnosis of interstitial lung diseases.

    abstract：:Bronchoalveolar lavage (BAL) is a useful procedure for differential diagnosis of interstitial lung diseases (ILDs) and for identification of granulomatous lung diseases. We investigated a panel of biomarkers from BAL fluid of ILD patients to evaluate their utility in differentiating ILDs. Bronchoscopy with BAL was per...

    journal_title：Clinical and experimental medicine

    pub_type： 杂志文章

    doi：10.1007/s10238-020-00608-5

    authors： d'Alessandro M,Carleo A,Cameli P,Bergantini L,Perrone A,Vietri L,Lanzarone N,Vagaggini C,Sestini P,Bargagli E

    更新日期：2020-05-01 00:00:00

  • The acute phase response following implantation of triclosan-bonded vascular prostheses.

    abstract：OBJECTIVE:Infection of prosthetic material is a major complication of vascular surgery. Therapy for it includes implantation of antimicrobial prostheses bonded with different antimicrobial agents. These agents may, however, induce an acute phase reaction following implantation in the host, thus compromising follow-up o...

    journal_title：Clinical and experimental medicine

    pub_type： 杂志文章

    doi：10.1007/s10238-001-8008-1

    authors： Hernandez-Richter TM,Wichmann MW,Schrödl W,Angele MK,Heinritzi K,Schildberg FW

    更新日期：2001-03-01 00:00:00

  • Increased B7H4 tissue expression correlates with high CA19.9 serum levels and a worse prognosis of pancreatic adenocarcinoma.

    abstract：:Pancreatic cancer (PC) is a leading cause of cancer death worldwide, especially in Western societies. Its aggressive nature and poor prognosis increase the need for identifying new and more accurate diagnostic and prognostic tools. We studied 41 patients who had undergone radical surgical resection for PC, investigate...

    journal_title：Clinical and experimental medicine

    pub_type： 杂志文章

    doi：10.1007/s10238-015-0352-7

    authors： Tsiaousidou A,Tsaroucha AK,Lambropoulou M,Pitiakoudis M,Polychronidis A,Chatzitheoklitos E,Romanidis K,Simopoulos C

    更新日期：2016-08-01 00:00:00

  • Megalin and cubilin in the human gallbladder epithelium.

    abstract：:Although the role of cholesterol absorption by the gallbladder epithelium in gallstone formation is well established, the exact process is poorly understood. Potential candidates for regulation of transepithelial cholesterol transport are suggested to be two large membrane multiple ligand receptors, megalin and cubili...

    journal_title：Clinical and experimental medicine

    pub_type： 杂志文章

    doi：10.1007/s10238-008-0174-y

    authors： Tsaroucha AK,Chatzaki E,Lambropoulou M,Despoudi K,Laftsidis P,Charsou C,Polychronidis A,Papadopoulos N,Simopoulos CE

    更新日期：2008-09-01 00:00:00

  • Effect of the antioxidant alpha-lipoic acid on apoptosis in human umbilical vein endothelial cells induced by high glucose.

    abstract：:High glucose plays an important role in the pathogenesis of atherosclerosis. In this study, we assessed the effects of high glucose on human umbilical vein endothelial cell (HUVEC) apoptosis. Additionally, we investigated whether alpha-lipoic acid, an antioxidant, prevents high glucose-induced apoptosis of HUVECs. HUV...

    journal_title：Clinical and experimental medicine

    pub_type： 杂志文章

    doi：10.1007/s10238-008-0155-1

    authors： Meng X,Li ZM,Zhou YJ,Cao YL,Zhang J

    更新日期：2008-03-01 00:00:00

  • A simple new method to calculate small intestine absorptive surface in the rat.

    abstract：:The rat is an established model for studying intestinal adaptations following abdominal surgery. In the study of functional and morphological adaptations of the small intestine, it is helpful to estimate the mucosal surface area. In order to simplify measurements and calculation we developed a new mathematical model f...

    journal_title：Clinical and experimental medicine

    pub_type： 杂志文章

    doi：10.1007/s102380200018

    authors： Kisielinski K,Willis S,Prescher A,Klosterhalfen B,Schumpelick V

    更新日期：2002-11-01 00:00:00

  • Administration of pigment epithelium-derived factor prolongs bleeding time by suppressing plasminogen activator inhibitor-1 activity and platelet aggregation in rats.

    abstract：:We have recently found that pigment epithelium-derived factor (PEDF), a glycoprotein with potent neuronal differentiating activity, not only inhibits endothelial cell damage, smooth muscle cell proliferation and platelet aggregation in vitro, but also suppresses occlusive thrombus formation in rats. These observations...

    journal_title：Clinical and experimental medicine

    pub_type： 杂志文章

    doi：10.1007/s10238-008-0010-4

    authors： Yamagishi S,Matsui T,Nakamura K,Takenaka K

    更新日期：2009-03-01 00:00:00

  • CCR2-V64I genetic polymorphism: a possible involvement in HER2+ breast cancer.

    abstract：:Many tumor cells express chemokines and chemokine receptors, and these molecules can affect both tumor progression and anti-tumor immune response. Genetic polymorphisms of some chemokine receptors were found to be closely related to malignant tumors, especially in metastasis process, including breast cancer (BC). Cons...

    journal_title：Clinical and experimental medicine

    pub_type： 杂志文章,评审

    doi：10.1007/s10238-015-0342-9

    authors： Banin-Hirata BK,Losi-Guembarovski R,Oda JM,de Oliveira CE,Campos CZ,Mazzuco TL,Borelli SD,Ceribelli JR,Watanabe MA

    更新日期：2016-05-01 00:00:00

  • Fuzzy logic-based tumor marker profiles improved sensitivity of the detection of progression in small-cell lung cancer patients.

    abstract：:Tumor markers were used for disease monitoring in small-cell lung cancer patients. The aim of this study was to improve diagnostic efficiency in the detection of tumor progression in small-cell lung cancer patients by using fuzzy logic modeling in combination with a tumor marker panel (NSE, ProGRP, Tumor M2-PK, CYFRA ...

    journal_title：Clinical and experimental medicine

    pub_type： 杂志文章

    doi：10.1007/s102380300005

    authors： Schneider J,Peltri G,Bitterlich N,Philipp M,Velcovsky HG,Morr H,Katz N,Eigenbrodt E

    更新日期：2003-02-01 00:00:00

  • Synergistic effect of TRAIL and irradiation in elimination of glioblastoma stem-like cells.

    abstract：:Glioblastoma multiforme (GBM) is the most common malignancy in central nervous system. A small subpopulation of GBM cells known as GBM stem-like cells (GSLCs) were supposed to be the most malignant cells among GBM cells as they are resistant to multiple therapies including radiotherapy. In this study, we set up two GS...

    journal_title：Clinical and experimental medicine

    pub_type： 杂志文章

    doi：10.1007/s10238-018-0504-7

    authors： Liu J,Gao Q,Xie T,Liu Y,Luo L,Xu C,Shen L,Wan F,Lei T,Ye F

    更新日期：2018-08-01 00:00:00

  • Diverse phenotypic and genotypic presentation of RAG1 mutations in two cases with SCID.

    abstract：:Severe combined immunodeficiencies (SCID) comprise a spectrum of genetic defects that involve both humoral and cellular immunities. Defects in recombinating activating gene 1 (RAG1), RAG2, Artemis, or LIG4 can disrupt V(D)J recombination. Defective V(D)J recombination of the T and B cell receptors is responsible for T...

    journal_title：Clinical and experimental medicine

    pub_type： 杂志文章

    doi：10.1007/s10238-009-0053-1

    authors： Karaca NE,Aksu G,Genel F,Gulez N,Can S,Aydinok Y,Aksoylar S,Karaca E,Altuglu I,Kutukculer N

    更新日期：2009-12-01 00:00:00