Abstract：
:Cancer stem cells (CSCs) are thought to be a major player in tumor initiation, progression, and metastasis. Targeting CSCs for elimination presents a promising therapeutic strategy; however, this approach will require a stronger understanding of CSC biology and identification of CSC-specific markers. The present study was conducted to examine the correlation between DCLK1 and miR-137 and miR-15a levels in colorectal cancer. A total of 222 samples, including 181 colorectal cancer specimens, 24 adenomatosis, and 17 non-adenomatosis colonic polyps, were stained for DCLK1 expression using immunohistochemistry. Also, expression of miR-137 and miR-15a was assessed in colorectal cancer with high and low DCLK1 expression levels. Most colorectal cancer specimens (76%) showed strong expression of DCLK1, whereas only 21% of adenomatous and none of non-adenomatous colonic polyps showed strong DCLK1 expression. A significant difference in DCLK1 expression was found between colorectal cancer, adenomatous, and non-adenomatous colonic polyps (P < 0.001). Higher expression of DCLK1 was more frequently detected in colorectal cases with larger tumor size (P = 0.03), poor differentiation (P = 0.03), and lymph node involvement (P = 0.04). Comparison of miR-137 and miR-15a in colorectal cancer cases revealed a significant inverse correlation with DCLK1 expression (P = 0.03 and P = 0.04, respectively). DCLK1 may act as a candidate marker for colorectal cancer stem cells. The critical role of DCLK1 in colorectal cancer suggests that it may represent an early diagnostic marker and therapeutic target; however, further investigation is warranted.
journal_name
Clin Exp Medjournal_title
Clinical and experimental medicineauthors
Razi S,Sadeghi A,Asadi-Lari Z,Tam KJ,Kalantari E,Madjd Zdoi
10.1007/s10238-020-00665-wsubject
Has Abstractpub_date
2020-09-23 00:00:00eissn
1591-8890issn
1591-9528pii
10.1007/s10238-020-00665-wpub_type
杂志文章abstract：:TNF-α plays a key role in the inflammatory cytokine cascade involved in the pathogenesis of chronic arthritis, including rheumatoid arthritis, psoriatic arthritis, and ankylosing spondylitis. Systemic inflammation and the increased production of pro-inflammatory cytokines in these patients may favor the onset of metab...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章,评审
doi：10.1007/s10238-014-0323-4
更新日期：2015-11-01 00:00:00
abstract：:To investigate the expression of cyclin G1, cyclin G2 in nasopharyngeal carcinoma (NPC) cell lines and its significance. The protein expression of cyclin G1, cyclin G2 in NPC cell lines of different differentiation degree (HNE2, CNE1) was detected by indirect immunofluorescence. The mRNA expression of cyclin G1, cycli...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-011-0142-9
更新日期：2012-03-01 00:00:00
abstract：:Although the role of cholesterol absorption by the gallbladder epithelium in gallstone formation is well established, the exact process is poorly understood. Potential candidates for regulation of transepithelial cholesterol transport are suggested to be two large membrane multiple ligand receptors, megalin and cubili...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-008-0174-y
更新日期：2008-09-01 00:00:00
abstract：:We have previously found that ex vivo expanded human CD4+CD25+Treg cells suppress proliferation of lymphoma B-cell lines. Here we demonstrate that the immunomodulatory drug lenalidomide potentiates suppression of lymphoma B-cell proliferation by freshly isolated CD4+CD25+Tregs, as well as suppression by Tregs expanded...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-016-0411-8
更新日期：2017-05-01 00:00:00
abstract：:Alpha1-adrenoceptor antagonists (alpha1-blockers) are currently used as first-line drugs for the treatment of benign prostatic hyperplasia (BPH). However, cases of BPH are often encountered in which the efficacy of alpha1-blockers decreases and switching to surgical treatment is required. One factor responsible for th...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-009-0073-x
更新日期：2010-06-01 00:00:00
abstract：:Mesenchymal stem cells (MSCs) have captured the attention of researchers today due to their multipotent differentiation capacity. Also, they have been successfully applied clinically, in the treatment of various diseases of the heart and musculoskeletal systems, with encouraging results. Their supportive role in haema...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章,评审
doi：10.1007/s10238-013-0247-4
更新日期：2014-08-01 00:00:00
abstract：:Recent findings suggest that cell and gene transplantation in the infarcted myocardium may improve heart function. The aim of the study was to investigate the mechanism involved in improving heart function following the co-injection of adipose-derived stem cells (ADSCs) and basic fibroblast growth factor (bFGF) in a r...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-015-0383-0
更新日期：2016-11-01 00:00:00
abstract：:Telomere shortening occurs with human aging in many organs and tissues and is accelerated by rapid cell turnover and oxidative injury. To clarify the clinical importance of telomere shortening in colonic mucosa in ulcerative colitis (UC), we measured average telomere length using quantitative real-time PCR in non-neop...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-014-0295-4
更新日期：2015-08-01 00:00:00
abstract：:Immunophenotypic characterization of the leukemic cells has been widely used as a tool for diagnosis, classification and prognosis of leukaemia. A total of 192 Chinese patients with acute myeloid leukemia (AML) were immunophenotyped by flow cytometry using a panel of monoclonal antibodies. Among the 192 patients enrol...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-009-0030-8
更新日期：2009-06-01 00:00:00
abstract：:SOCS3 as an important negative regulator of IL6/JAK/STAT3 signaling pathway may be early critical determinants of carcinogenesis. This study aimed to explore the aberrant promoter methylation of SOCS3 gene in circulating DNA as a noninvasive biomarker for screening hepatocellular carcinoma (HCC) high-risk individuals ...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-017-0473-2
更新日期：2018-02-01 00:00:00
abstract：:Bronchoalveolar lavage (BAL) is a useful procedure for differential diagnosis of interstitial lung diseases (ILDs) and for identification of granulomatous lung diseases. We investigated a panel of biomarkers from BAL fluid of ILD patients to evaluate their utility in differentiating ILDs. Bronchoscopy with BAL was per...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-020-00608-5
更新日期：2020-05-01 00:00:00
abstract：:The thrombin generation potential is an in vitro measure for the capacity of an individual to generate thrombin and recognized as a reflection of a hypo- or hypercoagulable status. Measurement of the in vivo thrombin activity, however, may be of clinical significance. We evaluated a new assay for in vivo thrombin acti...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-016-0417-2
更新日期：2017-05-01 00:00:00
abstract：:The background of this article is as follows: Few data are available about the persistence of serum-specific IgG antibodies to L. infantum after acute VL. The objective of this article is to evaluate the persistence of antibodies against L. infantum in patients healed from acute VL, and the kinetic of the same antibod...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-011-0150-9
更新日期：2012-06-01 00:00:00
abstract：:Atopic dermatitis (AD) is a chronic, inflammatory skin disease which is characterized by severe pruritus and affects patients' quality of life. In recent years gamma interferon (IFN-gamma) has been accepted as a novel treatment for severe AD, however, its mechanism of action is not clearly identified. Present study ev...
journal_title：Clinical and experimental medicine
pub_type： 临床试验,杂志文章
doi：10.1007/s10238-011-0164-3
更新日期：2012-12-01 00:00:00
abstract：:To investigate lncRNAs acting as competing endogenous RNAs (ceRNAs) involved in oncogenesis and progression of HCC. Different expressed lncRNAs, microRNAs, and mRNAs (DElncRNAs, DEmiRNAs, DEmRNAs), downloaded from The Cancer Genome Atlas (TCGA) database, were identified by edgeR package. CeRNA network was constructed ...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-020-00634-3
更新日期：2020-08-01 00:00:00
abstract：:Systemic lupus erythematosus (SLE) is the prototype autoimmune rheumatic disease. The etiology of this disease is incompletely understood; however, environmental factors and genetic predisposition are involved. Cytokine-mediated immunity plays a crucial role in the pathogenesis of SLE. We investigate the association o...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-014-0315-4
更新日期：2015-11-01 00:00:00
abstract：:The objective of this study was to compare between the effects of mesenchymal stem cell (MSC) and betamethasone in the treatment of rheumatoid arthritis. Sixty male albino mice were divided equally into 2 models. They are MSC model, group 1: saline control group, group 2: collagen-induced arthritis (CIA), group 3: ind...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-013-0248-3
更新日期：2014-08-01 00:00:00
abstract：PURPOSE:Exosome-derived long non-coding RNAs (lncRNAs) as novel biomarkers are widely investigated in various cancers, yet results remain controversial. The aim of this meta-analysis was to clarify the diagnostic and prognostic value of exosome-derived lncRNAs in cancer. METHODS:PubMed, Web of Science, EMBASE, CNKI, a...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章,评审
doi：10.1007/s10238-020-00638-z
更新日期：2020-08-01 00:00:00
abstract：:Anti-tumour necrosis factor-α (TNF-α) therapy brought new hopes for treating rheumatic diseases but also increased the risk of infection, including mycobacterium tuberculosis (MTb). Conventional screening tools, such as tuberculin skin test (TST), lack sensitivity or specificity. Recently, T-SPOT.TB has been introduce...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-010-0123-4
更新日期：2011-09-01 00:00:00
abstract：:Breast cancer is the most common cause of malignancy and mortality in women worldwide. This study aimed at localising homologous recombination repair (HR) genes and their chromosomal loci and correlating their nucleotide variants with susceptibility to breast cancer. In this study, authors analysed the association bet...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-016-0441-2
更新日期：2017-11-01 00:00:00
abstract：:The incidence of sepsis-associated acute kidney injury (AKI) is on the rise. Recent studies have found a correlation between antithrombin III and AKI. We established a predictive model for sepsis-associated AKI based on plasma ATIII levels. A prospective study (March 2018-January 2020) was conducted in sepsis patients...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-020-00656-x
更新日期：2020-08-31 00:00:00
abstract：:Glioblastoma multiforme (GBM) is the most common malignancy in central nervous system. A small subpopulation of GBM cells known as GBM stem-like cells (GSLCs) were supposed to be the most malignant cells among GBM cells as they are resistant to multiple therapies including radiotherapy. In this study, we set up two GS...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-018-0504-7
更新日期：2018-08-01 00:00:00
abstract：:The purpose of this study was to reveal the clinical diagnostic and prognostic value of miR-768-3p in HBV-related HCC and to investigate its effect on the biological function of HCC. Quantitative real-time polymerase chain reaction was used to detect the expression level of miR-768-3p in subjects' serum. The receiver ...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-020-00646-z
更新日期：2020-11-01 00:00:00
abstract：:Increased expression of progranulin (PGRN) has been reported in some hematological cancers, but limited information regarding its significance in acute lymphoblastic leukemia (ALL) is available. This study involved 60 subjects (40 de novo adult ALL patients and 20 controls). Serum PGRN level was measured by enzyme-lin...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-020-00610-x
更新日期：2020-05-01 00:00:00
abstract：:This paper describes the clinical significance of antibodies to the ribosomal P proteins in systemic lupus erythematosus. It appears that liver disease due to the lupus process and not attributable to viral infection, alcohol or drugs is associated with anti-ribosomal P. In addition, there is a strong relationship to ...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章,评审
doi：10.1007/s10238-006-0094-7
更新日期：2006-06-01 00:00:00
abstract：OBJECTIVE:Infection of prosthetic material is a major complication of vascular surgery. Therapy for it includes implantation of antimicrobial prostheses bonded with different antimicrobial agents. These agents may, however, induce an acute phase reaction following implantation in the host, thus compromising follow-up o...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-001-8008-1
更新日期：2001-03-01 00:00:00
abstract：:Pancreatic islet transplantation has remained an effective therapy for type 1 diabetes since 2000. Its widespread use has been prohibited by the shortage of suitable donors. It is critical to explore an applicable alternative for β-cell replacement. This study was performed to generate insulin-producing cells (IPCs) f...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-014-0319-0
更新日期：2015-11-01 00:00:00
abstract：:B-cell chronic lymphocytic leukemia (B-CLL) is one of the most common leukemias among the elderly and, despite many efforts, still stays incurable. Recent studies point to the microenvironment as the critical factor providing leukemic lymphocytes with pro-survival signals. Thus, the neighboring cells appear to be a pe...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-013-0268-z
更新日期：2015-02-01 00:00:00
abstract：:Studies have demonstrated cytomegalovirus (CMV) DNA particles in restenotic lesions in atherosclerotic coronary arteries. We have shown that high (>1:800) anti-CMV IgG antibody titers in the serum are associated with active coronary disease and with post coronary angioplasty restenosis. In this study we assessed the a...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-003-0019-7
更新日期：2003-11-01 00:00:00
abstract：:The present study was initiated to examine whether the concentration of CO in the breath is elevated in patients with inflammatory bowel disease (IBD). Twenty-three clinically stable patients with IBD in the outpatient clinic (11 with Crohn's disease, 12 with ulcerative colitis), who are non-smokers and non-passive sm...
journal_title：Clinical and experimental medicine
pub_type： 杂志文章
doi：10.1007/s10238-007-0129-8
更新日期：2007-06-01 00:00:00
