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PDX-1 mRNA-induced reprogramming of mouse pancreas-derived mesenchymal stem cells into insulin-producing cells in vitro.

Abstract：

:Pancreatic islet transplantation has remained an effective therapy for type 1 diabetes since 2000. Its widespread use has been prohibited by the shortage of suitable donors. It is critical to explore an applicable alternative for β-cell replacement. This study was performed to generate insulin-producing cells (IPCs) from pancreas-derived mesenchymal stem cells (pMSCs). pMSCs were isolated from discarded pancreatic tissue in the filter liquor during islet isolation procedure in mice and ex vivo expanded in culture. IPCs were induced by transfection of pancreas and duodenal transcription factor 1 (PDX-1) mRNA in vitro. Some islet characteristics were identified on pMSC-derived IPCs in mRNA and protein levels. Our results demonstrated that mouse pMSCs can be transdifferentiated into effective glucose-responsive insulin-producing cells through transfecting synthetic modified PDX-1 mRNA in vitro. The study of PDX-1 mRNA-induced pMSC reprogramming may pave the way toward the development of a novel β-cell source for the treatment of diabetes.

journal_name

Clin Exp Med

journal_title

Clinical and experimental medicine

authors

Guo XR,Wang XL,Li MC,Yuan YH,Chen Y,Zou DD,Bian LJ,Li DS

doi

10.1007/s10238-014-0319-0

subject

Has Abstract

pub_date

2015-11-01 00:00:00

pages

501-9

issue

4

eissn

1591-8890

issn

1591-9528

pii

10.1007/s10238-014-0319-0

journal_volume

15

pub_type

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