Abstract：
:Histidyl-proline diketopiperazine (cyclo(His-Pro), a metabolite of the neuropeptide thyrotropin releasing hormone, has been shown to possess intrinsic biological activities. The binding of this peptide to various tissue particulate preparations was investigated. While the peptide showed no apparent binding to particulate fractions derived from brain, pituitary, and some other tissues, binding to adrenal and liver was demonstrated. The binding of cyclo(His-Pro) to bovine adrenal cortical particles was further characterized. Binding at equilibrium was greater at 4 degrees C than at 37 degrees C. The binding was dependent on tissue concentration, showed a pH optimum between 7 and 8, and was inactivated by treatment of the particulate fraction with trypsin or by boiling. The interaction of cyclo(His-Pro) with the tissue was not associated with any metabolism of the peptide. Kinetic studies of association of cyclo(His-Pro) with adrenal cortical particles indicated a single class of binding sites with a KD of approximately 900 nM and a maximum number of sites of 92 pmoles/mg protein. The binding was stereospecific and the histidine moiety of the peptide was the major determinant of the binding. A variety of catechols, serotonin and histamine competed with cyclo(His-Pro) for binding with IC50's ranging from 17-450 muM. Cyclo(His-Pro) did not affect monoamine oxidase or adenylate cyclase activity in adrenal cortical particulate preparations.
journal_name
Peptidesjournal_title
Peptidesauthors
Bataini F,Koch Y,Takahara Y,Peterkofsky Adoi
10.1016/0196-9781(83)90172-9subject
Has Abstractpub_date
1983-01-01 00:00:00pages
89-96issue
1eissn
0196-9781issn
1873-5169pii
0196-9781(83)90172-9journal_volume
4pub_type
杂志文章相关文献
PEPTIDES文献大全abstract：:As we commemorate the 25th anniversary of the journal Peptides, it is timely to review the functional significance of the bombesin (BB)-like peptides and receptors in the CNS. Over two decades ago we published an article in the journal Peptides demonstrating that BB-like peptides are present in high densities in certa...
journal_title：Peptides
pub_type： 杂志文章,评审
doi：10.1016/j.peptides.2004.02.012
更新日期：2004-03-01 00:00:00
abstract：:High performance-gel permeation chromatography (HP-GPC) evaluation of human plasma and serum incubated at 37 degrees C up to 24 h after the addition of long-acting natriuretic peptide (LANP) revealed a 25% decrease in LANP's peak at 2 h followed by an increase in plasma without EDTA at 8 h (p < 0.001). Vessel dilator'...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/s0196-9781(96)00185-4
更新日期：1996-01-01 00:00:00
abstract：:An antifungal protein was isolated from the mushroom Tricholoma giganteum var. golden blessings. The protocol included ion exchange chromatography on DEAE-cellulose, affinity chromatography on Affi-gel blue gel, ion exchange chromatography on CM-cellulose, and gel filtration by fast protein liquid chromatography on Su...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2004.11.009
更新日期：2005-04-01 00:00:00
abstract：:Recent pharmacological data strongly support the hypothesis of delta receptor subtypes as mediators of both supraspinal and spinal antinociception (delta 1 and delta 2 receptors). In vitro ligand binding data, which are fully supportive of the in vivo data, are still lacking. A previous study indicated that [3H][D-Ala...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(92)90030-7
更新日期：1992-11-01 00:00:00
abstract：:VIP was given intravenously over 1 min at the doses 0.1 and 0.2 micrograms X kg X min-1 to twenty-one anesthetized patients undergoing abdominal surgery. Intra-arterial blood pressure was monitored and various blood flows were measured simultaneously by electromagnetic technique. Following VIP, intra-arterial blood pr...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(84)90227-4
更新日期：1984-03-01 00:00:00
abstract：:The effects of Pro-Leu-Gly-NH2 (melanotropin release inhibiting factor, MIF) and its analog, cyclo (Leu-Gly) on the mouse and rat striatal cholinergic muscarinic receptors labeled with 3H-quinuclidinyl benzilate (QNB) were investigated. 3H-QNB bound to the rat striatal muscarinic receptors at a single high affinity si...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(86)90055-0
更新日期：1986-01-01 00:00:00
abstract：:The effects of arginine vasotocin (AVT), 4-6 ng/kg, on renal function and plasma prolactin (PRL) were determined in conscious American foxhounds undergoing a water diuresis. Intravenous AVT produced an antidiuresis, kaliuresis and inconsistent natriuresis, independently of changes in GFR, blood pressure, heart rate an...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(82)90062-6
更新日期：1982-11-01 00:00:00
abstract：:Intracerebroventricular administration of NPY suppresses ethanol intake in selectively bred alcohol-preferring rat lines, but not in rats selectively bred for low ethanol drinking or in unselected Wistar rats, when access to ethanol is limited to 2h/day. However, when rats undergo chronic (24h/day) ethanol drinking (o...
journal_title：Peptides
pub_type： 杂志文章,评审
doi：10.1016/j.peptides.2006.07.028
更新日期：2007-02-01 00:00:00
abstract：:The hypotensive effect of proadrenomedullin N-terminal 20 peptide (PAMP) was examined in conscious pregnant (8, 14, and 20 days of pregnancy) and nonpregnant rats. Intravenous administration of PAMP (3-60 nmol/kg) produced a dose-dependent depressor response in both pregnant and nonpregnant rats. However, the maximum ...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/s0196-9781(99)00165-5
更新日期：1999-12-01 00:00:00
abstract：:Brain-derived neurotrophic factor-like neuropeptide is produced in the brain of the silk moth, Bombyx mori. Immunocytochemical studies of brain and retrocerebral complex of larvae, prepupae, pupae and adults showed that four pairs of median neurosecretory cells and six pairs of lateral neurosecretory cells which had d...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2004.07.005
更新日期：2004-11-01 00:00:00
abstract：:We examined the effect of adjuvant arthritis on the content of immunoreactive calcitonin gene-related peptide (iCGRP) in the dorsal root ganglia at L4-L6 levels and the spinal cord at a lumbar level in rats. Arthritis was induced by inoculating adjuvant into both hind-paws twice at a 10 day interval. In the arthritic ...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(89)90057-0
更新日期：1989-03-01 00:00:00
abstract：:A novel antimicrobial peptide, with molecular mass of 1602.0469Da, produced by Brevibacillus laterosporus strain A60 was isolated and purified from the soil of mango plants. The purification procedure consisted of ammonium sulfate precipitation, cation exchange chromatography on an HiTrap SP HP column, thin layer chro...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2012.01.001
更新日期：2012-02-01 00:00:00
abstract：:In the Nucleus Ambiguus (NA) mu-receptors increased mean arterial pressure (MAP) and heart rate (HR) and kappa-receptors decreased MAP without altering HR. Mu-receptors in the Dorsal Motor Nucleus of the Vagus (DMV) increased MAP without changing HR whereas kappa-receptors in the DMV decreased HR without changing MAP....
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(88)90225-2
更新日期：1988-01-01 00:00:00
abstract：:Alternaria alternata protein, Alt a 1 is a major allergen associated with allergy in atopic patients. Although the molecule binds strongly to IgE antibody from patients, the epitopes involved have not been identified or defined. In the present study, we synthesized overlapping peptides spanning the whole sequence and ...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/s0196-9781(03)00024-x
更新日期：2003-02-01 00:00:00
abstract：:Malaria lactate dehydrogenase, a glycolytic enzyme, is a malaria diagnostic target in lateral flow immunochromatographic rapid diagnostic tests. Recombinant Plasmodium yoelii LDH was cloned into the pET-28a vector, expressed and the expressed protein purified from a Ni-NTA affinity matrix. A pan-malarial LDH antibody ...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2010.01.002
更新日期：2010-04-01 00:00:00
abstract：:Strips of lower esophageal sphincter (LES) from rabbits were tested for their responses to several peptides, and to electrical field stimulation (EFS) under the presence of some peptides. Substance P (SP), motilin, and bombesin induced contraction, and vasoactive intestinal peptide (VIP) induced relaxation. SP- and bo...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(96)00137-4
更新日期：1996-01-01 00:00:00
abstract：:The presence of immunoreactive endothelin (IR-ET) in human saliva and rat parotid gland was investigated by radioimmunoassay. The IR-ET concentration (mean +/- SEM) in saliva taken from normal volunteers was 2.0 +/- 0.2 pmol/l (n = 15). The IR-ET concentration in rat parotid gland was 19.2 +/- 2.2 fmol/g wet weight (n...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(91)90151-e
更新日期：1991-07-01 00:00:00
abstract：:The melanocortin 4 receptor (MC4R) plays an essential role in weight regulation. Recently we could show that the MC4R is able to form receptor dimers. In the present study we investigated the role of extracellular cysteine residues and the structure of the third extracellular loop for receptor dimerization. None of th...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2005.02.027
更新日期：2006-02-01 00:00:00
abstract：:The cholecystokinin receptor-type 1 (CCK1R) is a G-protein coupled receptor localized in the animal gastrointestinal tract. Receptor activation by the natural peptide ligand CCK leads to a feeling of satiety. In this study, hydrolysates from soy and milk proteins were evaluated for their potential to activate the CCK1...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2011.11.019
更新日期：2012-03-01 00:00:00
abstract：:Leptin has recently been discussed as a novel biomarker for the clinical outcome of critical illness. This study aims to investigate the prognostic value of leptin with regard to long-term clinical outcomes in patients with intracerebral hemorrhage. In 50 healthy controls and 92 patients with acute spontaneous basal g...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2013.03.010
更新日期：2013-05-01 00:00:00
abstract：:The extractability of neurotensin (NT) from porcine ileal mucosa was studied by comparison of eight extraction procedures. Tissue content of neurotensin-like immunoreactivity was quantitated and characterized by sequence-specific radioimmunoassays and gel filtration chromatography. Homogenization prior to boiling in e...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(87)90038-6
更新日期：1987-07-01 00:00:00
abstract：:Melanin-concentrating hormone (MCH) is a hypothalamic peptide that plays a critical role in the regulation of food intake and energy metabolism. In this study, we investigated the potential role of dense hippocampal MCH innervation in the spatially oriented food-seeking component of feeding behavior. Rats were trained...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2015.12.007
更新日期：2016-02-01 00:00:00
abstract：:The hypocretins (1 and 2) have emerged as key regulators of sleep and wakefulness. We developed a high-throughput enzyme immunoassay (EIA) to measure total brain hypocretin levels from large numbers of mice. Hypocretin levels were not altered by circadian time or age. However, significant differences in one or both hy...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/s0196-9781(02)00251-6
更新日期：2002-12-01 00:00:00
abstract：:Opioid receptor binding properties of [3H]Tyr-D-Ala-Phe-Phe-NH2 (TAPP) were characterized in rat brain and Chinese hamster ovary (CHO) cells expressing the rat mu-receptor. In rat brain, [3H]TAPP labeled a single class of opioid sites with a dissociation constant (Kd) of 0.31 nM and maximal number of binding sites (Bm...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/s0196-9781(98)00023-0
更新日期：1998-01-01 00:00:00
abstract：:Despite the high impact of the antimicrobial peptide hepcidin in iron homeostasis, the regulation of this hormone is still not completely understood. Studies concerning hepcidin regulation are performed at the mRNA level. For the first time we analyzed the regulation of hepcidin not only at mRNA, but also at protein l...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2007.01.016
更新日期：2007-05-01 00:00:00
abstract：:The binding activity of several galanin fragments and analogs was measured on specific receptors present in rat brain and the rat pancreatic beta cell line Rin m 5F. In both tissues it was observed that: 1) galanin(3-29), galanin(10-29) and [Ile2]-galanin were ineffective for inhibiting [125I] galanin binding and 2) a...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(89)90109-5
更新日期：1989-07-01 00:00:00
abstract：:Pituitary adenylate cyclase-activating polypeptide (PACAP) has a similar structure to that of vasoactive intestinal peptide (VIP) and both the polypeptides belong to the same molecular group, the secretin-glucagon superfamily. PACAP and VIP have possible potency as hypothalamic factors mediating the release of pituita...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/j.peptides.2005.02.022
更新日期：2005-09-01 00:00:00
abstract：:Biological and immunological relationships between molt-inhibiting hormone (MIH) activity in eyestalk ganglia extracts of the crab, Cancer antennarius Stimpson, and peptides of the vasopressin-oxytocin family were assessed. Lysine vasopressin (LVP), arginine vasopressin (AVP), vasotocin (VT), and oxytocin (OT) mimicke...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(85)90166-4
更新日期：1985-07-01 00:00:00
abstract：:Bombyxin-IV, a molecular species of bombyxin, which is a member of insulin-like heterodimeric peptides of the silkworm Bombyx mori with prothoracicotropic hormone activity, was synthesized. The A- and B-chains of bombyxin-IV containing four and two Cys residues, respectively, were first synthesized separately by solid...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(90)90126-p
更新日期：1990-01-01 00:00:00
abstract：:Neural membrane fractions, prepared from brain-subesophageal ganglion complexes of the adult lepidopteran Lymantria dispar, contain at least two peptidases capable of metabolizing locust adipokinetic hormone-I in vitro. The initial fragments, pGlu1-Leu2-Asn3 and Phe4-Thr5-Pro6-Asn7-Trp8-Gly9-Thr10, result from the act...
journal_title：Peptides
pub_type： 杂志文章
doi：10.1016/0196-9781(95)02098-5
更新日期：1996-01-01 00:00:00
