Abstract：
:A novel antimicrobial peptide, with molecular mass of 1602.0469Da, produced by Brevibacillus laterosporus strain A60 was isolated and purified from the soil of mango plants. The purification procedure consisted of ammonium sulfate precipitation, cation exchange chromatography on an HiTrap SP HP column, thin layer chromatography and High Performance Liquid Chromatography (HPLC) on C18 reversed-phase column. After the four isolation procedures, one peptide with antimicrobial activity was obtained and named BL-A60. The determination of the complete amino acid sequences of this peptide showed that it contains eleven amino acid residues, L-Y-K-L-V-K-V-V-L-N-M, and a choline connected to the N-terminal and a tenuazonic acid modified of the C-terminal. This peptide shows relatively low identification to other antimicrobial peptides from bacteria. Purified BL-A60 showed high pH and thermal stability and a strong inhibition of different stages of the life cycle of Phytophthora capsici, including mycelial growth, sporangia formation and cystospore germination, with EC(50) values of 7.89, 0.60 and 21.96 μg ml(-1), respectively.
journal_name
Peptidesjournal_title
Peptidesauthors
Zhao J,Guo L,Zeng H,Yang X,Yuan J,Shi H,Xiong Y,Chen M,Han L,Qiu Ddoi
10.1016/j.peptides.2012.01.001subject
Has Abstractpub_date
2012-02-01 00:00:00pages
206-11issue
2eissn
0196-9781issn
1873-5169pii
S0196-9781(12)00007-1journal_volume
33pub_type
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