Abstract：
:Normal adult albino and Sprague-Dawley rats, under intraperitoneal Nembutal anesthesia, were used to demonstrate enzymatic activity in the choroid plexus and ventricular ependyma. The brain tissues were perfused or immersed with cold 2% glutaraldehyde and 8% sucrose in 0.1 M cacodylate buffer (pH 7.2-7.4) for 30 min and washed overnight in the same buffer solution., The choroid plexus (lateral and fourth ventricles) and ventricular ependyma (lateral ventricle) were trimmed from the fixed and washed brain tissues, which were frozen and sectioned. For histo- and cyto-chemical study, the sections were immersed in the following incubation media; for Na+, K+-ATPase (ouabain-sensitive, K+-dependent, p-nitrophenylphosphatase: p-NPPase) according to the one-step method of Mayahara et al. (1978): for Mg2+- ATPase, Wachstein-Meisel's incubation medium (1957); for adenylate cyclase (AC), following Araki and Saito's lead citrate method (1979). The cytochemical findings gave the following results. In the choroid plexus, the ouabain-sensitive electron-dense reaction products of NA+, K+-ATPase (p-NPPase) were strongly positive in the microvilli and along the inner surface of microvilli, without showing any Mg2+-ATPase and AC activities, and all three enzymatic activities were positive along the basal plasmalemmas and negative along the lateral and apical (not including microvilli) plasmalemmas. In the ventricular ependyma, Na+,K+-ATPase (P-NPPase) activity was not found, and the reaction product of AC was observed on the apical plasmalemmas and those of Mg2+-ATPase along the basal plasmalemmas. These cytochemical findings are helpful in understanding the regulation of cerebrospinal fluid production through Na+, K+-ATPase (p-NPPase) and cyclic AMP (AC).
journal_name
Brain Resjournal_title
Brain researchauthors
Masuzawa T,Saito T,Sato Fdoi
10.1016/0006-8993(81)91035-0subject
Has Abstractpub_date
1981-10-19 00:00:00pages
309-22issue
2eissn
0006-8993issn
1872-6240pii
0006-8993(81)91035-0journal_volume
222pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:The BE (2)-M17 human neuroblastoma has previously been shown to express corticotropin-releasing factor (CRF) mRNA following retinoic acid treatment. It is demonstrated in this report that both cell extracts and cell incubation medium of retinoic acid-treated BE (2)-M17 cells were shown to contain CRF-like immunoreacti...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)91583-0
更新日期：1994-08-15 00:00:00
abstract：:The N-methyl-D-aspartate (NMDA) subtype of excitatory amino acid receptor is linked to an ion channel that is blocked by the phencyclidine analog N-(1-[thienyl]cyclohexyl)piperidine (TCP). Previous studies have shown that NMDA and glycine act together to increase the access of [3H]TCP to its binding site, presumably b...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)90918-2
更新日期：1990-05-21 00:00:00
abstract：:Cortical stimulation has been demonstrated to alleviate certain pain conditions. The aim of this study was to determine the responses of the spinal cord dorsal horn neurons to stimulation of the primary somatosensory cortex (SSC). We hypothesized that direct stimulation of the SSC will inhibit the activity of spinal d...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2005.07.044
更新日期：2005-09-28 00:00:00
abstract：:To elucidate the intracellular mechanism of the bursting activity which is characteristic of seizure discharge, the behavior of the intracellular cyclic nucleotide and the intracellular calcium during pentylenetetrazole (PTZ)-induced bursting activity in snail neurons was investigated. Cyclic AMP was increased about 3...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(83)90137-3
更新日期：1983-06-20 00:00:00
abstract：:Experimental traumatic brain injury (TBI) is known to produce an acute increase in cerebral glucose utilization, followed rapidly by a generalized cerebral metabolic depression. The current studies determined effects of single or multiple treatments with sodium pyruvate (SP; 1000mg/kg, i.p.) or ethyl pyruvate (EP; 40m...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2016.04.005
更新日期：2016-07-01 00:00:00
abstract：:Mouse neopallium was disaggregated at 3 developmental stages (E15, E18, PO) and grown in colony cultures for 7 days. On the seventh day of culturing the colonies of cells were transplanted into the cerebellums of neonatal mice. After 3 weeks the astrocytes within the transplants were identified with GFAP immunoperoxid...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0165-3806(83)90096-2
更新日期：1983-01-01 00:00:00
abstract：:This study tested the effect on intracranially injected cycloheximide (CHX), an inhibitor of protein synthesis, on facilitation of sexual receptivity in ovariectomized rats. The rats received 0.5 microgram estradiol benzoate (EB), s.c. once daily on days 8 through 12 after ovariectomy (OVX). Either CHX (in 0.5 microli...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(81)91050-7
更新日期：1981-10-19 00:00:00
abstract：:Kinins, such as bradykinin and Lys-bradykinin, are important mediators in peripheral inflammation. Although the existence of the components necessary for generating kinins has been demonstrated in the brain, a functional role of the kinin-generating system in cerebral inflammation remains to be defined. The aim of the...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(03)02768-9
更新日期：2003-07-18 00:00:00
abstract：:Histamine is one of the neurotransmitters suppressing appetite, but the interactions of histaminergic neurons with other neurons in satiety centers have not been clarified. Noradrenaline in the para-ventricular nucleus (PVN) has been shown to stimulate feeding. This study was designed to determine whether histamine mo...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(99)01339-6
更新日期：1999-05-15 00:00:00
abstract：:Instructions are an inextricable, yet poorly understood aspect of modern human life. In this paper we propose that instruction implementation and following can be understood as fast Hebbian learning in prefrontal cortex, which trains slower pathways (e.g., cortical-basal ganglia pathways). We present a computational m...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2011.12.025
更新日期：2012-02-23 00:00:00
abstract：:Inorganic mercury (Hg2+) reduced voltage-gated calcium channel currents irreversibly in two different preparations. In cultured rat dorsal root ganglion (DRG) neurons, studied with the whole cell patch clamp technique, a rapid concentration-dependent decrease in the L/N-type currents to a steady state was observed wit...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(93)91146-j
更新日期：1993-12-31 00:00:00
abstract：:The responses of single alpha motoneurons to various "natural' stimuli were recorded from dissected ventral root filaments in lightly anesthetized control and recently decerebellate cats. The identity of the motoneurons was established by recording the unitary responses to orthodromic stimulation of nerves innervating...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(75)90208-5
更新日期：1975-09-12 00:00:00
abstract：:Picrotoxin-induced epileptiform activity was examined in neocortical slices prepared from 8- to 15-day-old rats. This activity consisted of spontaneous bursts of 3-5 discharges that resembled interictal spikes and were interspersed with ictal-like paroxysms lasting 10-30 s. Measurements of extracellular potassium ([K+...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0165-3806(87)90036-8
更新日期：1987-12-01 00:00:00
abstract：:Exercise has been shown to influence learning and memory. Most studies were performed with a voluntary running paradigm (e.g. running wheel) in mice. However, such effects of exercise on learning and memory are less well demonstrated using a forced running paradigm (e.g. treadmill). The present study was designed to e...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2006.07.023
更新日期：2006-10-03 00:00:00
abstract：:It is widely believed that substance use disorder (SUD) results from both pre-alterations (vulnerability) and/or post-alterations (drug effects) on cortico-striatal circuits. These circuits are essential for cognitive control, motivation, reward dependent learning, and emotional processing. As such, dysfunctions in co...
journal_title：Brain research
pub_type： 杂志文章,评审
doi：10.1016/j.brainres.2015.03.048
更新日期：2015-12-02 00:00:00
abstract：:Transependymal absorption of cerebrospinal fluid (CSF) in hydrocephalus is suggested by periventricular edema, but the necessary bulk flow of interstitial fluid (ISF) has not been found. We performed ventriculocisternal perfusions in adult cats using CSF with the extracellular marker [3H]sucrose. CSF pressure was main...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(82)90616-3
更新日期：1982-01-28 00:00:00
abstract：:The present study utilized the [14C]2-deoxyglucose (2-DG) cell labeling procedure to characterize a functional pathway from the prefrontal cortex (Pfc) and mediodorsal thalamic nucleus (MD) to the hypothalamus. Rats were injected with 2-DG prior to a 45 min experimental paradigm consisting of alternating 30 s on-off p...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(84)90151-3
更新日期：1984-09-24 00:00:00
abstract：:In this study intracellular recording techniques were used to study the synaptic events related to phase-locking of cochlear nucleus cells to low frequency stimuli. A variable degree of phase-locking was noted even with units of the same low characteristic frequency. With low frequency phase-locking units an excitator...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(76)90286-9
更新日期：1976-08-13 00:00:00
abstract：:Apart from the essential role of 1alpha,25-dihydroxyvitamin D3 in calcium and phosphorus metabolism, this compound and its analogs are involved in regulating the functions of the central nervous and immune systems. Active forms of vitamin D3 have been reported to stimulate neurotrophin gene expression and to prevent n...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2005.01.034
更新日期：2005-03-28 00:00:00
abstract：:We developed a rat spinal cord injury model and investigated whether endogenous endothelin (ET)-1 plays a role in astrocytic growth after injury. Immunohistochemical study showed that the number of immature astrocytes (ACs) exhibiting strong reactivity to the monoclonal antibody, RC1, markedly increased 24 h after the...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(96)00524-0
更新日期：1996-07-29 00:00:00
abstract：:Methamphetamine (METH) is one of the most commonly abused psychostimulant, and is known to induce dopaminergic neurotoxicity by generating oxidative stress and free radicals. In the present study we investigated the effects of METH on egr-1 and c-fos immediate early gene induction in different regions of mouse brain, ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(01)02991-2
更新日期：2001-11-16 00:00:00
abstract：:Fast axonal transport and neuronal protein synthesis was studied in the isolated nervous system of Aplysia californica. The abdominal ganglion with attached pleural-abdominal connectives (PAC) was removed and the ganglion pulse-labelled with [35S]methionine for 30 min in vitro. The axon containing connectives were lig...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(85)90388-9
更新日期：1985-04-15 00:00:00
abstract：:The effect of intracerebroventricular (i.c.v.) infusion of various iso- and hypertonic saccharide solutions on water intake stimulated by intracarotid (i.c.) infusion of hypertonic NaCl was studied in sheep. Without an i.c.v. infusion, water intake during a 10-min period following an i.c. infusion of 4 M NaCl (1.4 ml/...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)91006-8
更新日期：1989-07-24 00:00:00
abstract：:The effects of two specific tyrosine kinase inhibitors on long-term potentiation (LTP) in the dentate gyrus were investigated using anesthetized rats. I.c.v. injection of herbimycin A (1.74 nmol) or lavendustin A (1.31 nmol) before application of tetanic stimulation significantly inhibited the formation of LTP. Howeve...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(93)90315-e
更新日期：1993-09-03 00:00:00
abstract：:Previous studies have reported that high concentrations of homocysteine and lower concentrations of vitamins B6, B12, and folate increase the risk for cognitive decline and pathology in aging populations. In this cross-sectional study, high resolution magnetic resonance imaging (MRI) scans and a 3-day food diary were ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2008.01.030
更新日期：2008-03-14 00:00:00
abstract：:The accumulation of acid-labile CO2 was measured in the rat brain at early times after exposure to 30% CO2 in oxygen. In particular, the effect on CO2 accumulation of inhibiting brain carbonic anhydrase with acetazolamide was studied. For 57 rats, a rat head perfusion technique was used which permits control and rapid...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(80)90443-6
更新日期：1980-09-15 00:00:00
abstract：:In adult rodents, neuroblasts originating from the subventricular zone migrate tangentially through the rostral migratory stream (RMS) toward the olfactory bulb where they differentiate into interneurons. Neuroblasts in the RMS migrate in chains for a long distance along specifically arranged blood vessels which promo...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2018.08.010
更新日期：2018-11-01 00:00:00
abstract：:Effects of methamphetamine (MAP) on the extracellular neuropeptide Y (NPY) and noradrenaline (NA) levels were examined in the vicinity of the paraventricular nucleus (PVN) of freely moving rats by means of push-pull perfusion. The NA and NPY levels increased significantly in 30-60 min and reached the maximum level in ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)01343-1
更新日期：1996-01-22 00:00:00
abstract：:Basic fibroblast growth factor (FGF-2) isoforms of the adrenal medulla are differentially expressed during rat postnatal development. While the 18 and 23 kDa isoforms continuously rise towards the adult expression level, the 21 kDa isoform displays a peak expression at postnatal day 28. The peak expression of the 21 k...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(97)00341-7
更新日期：1997-05-23 00:00:00
abstract：:We have recently characterized the histopathological changes in an experimental model of mesial temporal lobe epilepsy (MTLE) induced by the intrahippocampal injection of low dose of kainate in mice. Although cerebral metabolism and blood flow are extensively studied and used in human MTLE to locate the regions involv...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(99)02092-2
更新日期：2000-01-10 00:00:00
