Abstract:
:We have developed a serum-free chemically defined medium which allows the obtainment of a primary culture highly enriched in ciliated ependymal cells. Serum was never used. Mechanically dissociated neonatal rat brain hemisphere cells were seeded on a fibronectin substratum. Culture medium was minimum Eagle's medium until day 14 in vitro and Waymouth's MD 705/l medium thereafter. Both media were supplemented with insulin (5 micrograms/ml), transferrin (10 micrograms/ml) and fatty acid-free bovine serum albumin (0.5 mg/ml). In these conditions, without addition of growth factors, the culture was enriched in ependymal cells; with the addition of thrombin cell growth was stimulated and moreover a nearly pure culture of ependymal cells was obtained. In this very simple culture medium, cells looked healthy up to two months after seeding and about 75% of the cells were ciliated. In a fraction of these cells the glial fibrillary acidic protein, a protein specific for astroglial cells in the central nervous system, was immunohistochemically revealed. The availability of an almost pure primary culture of ependymal cells will make many studies possible on this cell type, particularly studies on the development of these cells, on the regulation of their genetic expression and on their physiological function.
journal_name
Brain Resjournal_title
Brain researchauthors
Weibel M,Pettmann B,Artault JC,Sensenbrenner M,Labourdette Gdoi
10.1016/s0006-8993(86)80228-1subject
Has Abstractpub_date
1986-03-01 00:00:00pages
199-209issue
2eissn
0006-8993issn
1872-6240pii
S0006-8993(86)80228-1journal_volume
390pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract::Decreasing transsynaptic activity through surgical adrenal denervation or by medullary explantation, increases Leu-enkephalin immunoreactivity (Leu-Enk) and preproenkephalin mRNA (prepro-EK). Membrane depolarization prevents this rise. To determine whether depolarizing effects are mediated by intracellular movement of...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(88)91407-2
更新日期:1988-02-16 00:00:00
abstract::Natural stimulation was used to map the receptive fields of single cells recorded from the rostral medial portion of the dorsal accessory olive (rDAO) and the subjacent principal olive (PO) of the barbiturate anesthetized cat. Previous reports indicated a somatotopic mapping of the entire contralateral body within the...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(93)91742-b
更新日期:1993-03-12 00:00:00
abstract::The mechanism(s) underlying age-associated diminutions in the rat striatal D2 dopamine receptor (D2-receptor) number was investigated. The levels of D2-receptor mRNA in 4-, 12- and 18-month-old rat striata were found not to change. In contrast, the levels of 110 kDa protein, labeled with a D2-receptor specific antibod...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(92)90095-q
更新日期:1992-03-20 00:00:00
abstract::Catecholamine-containing fibers have been examined in the cerebella of normal and hypocerebellar mutant mice using Falck-Hillarp and glyoxylic acid histofluorescence techniques. The amounts of norepinephrine and dopamine were also determined chemically in the same mutants. Green fluorescent fibers in cerebella of norm...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(75)90349-2
更新日期:1975-08-08 00:00:00
abstract::Large neuronal perikarya isolated in bulk fractions from adult bovine ventral spinal cord and examined by electron microscopy have discontinuities in their plasma membrane. A systematic investigation of the possible causes of the membrane defects indicates that the act of mechanical dissociation of the neurons from th...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(78)90108-7
更新日期:1978-12-22 00:00:00
abstract::Methamphetamine is a highly addictive psychostimulant that has surged in popularity worldwide in the last decade. Brain-derived neurotrophic factor (BDNF), a member of the neurotrophic factor family, is widely expressed in the adult mammalian brain and plays an important role in the long-term survival, differentiation...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2010.08.053
更新日期:2010-10-21 00:00:00
abstract::6-[18F]Fluoro-L-DOPA (FDOPA) is an imaging agent used in the study of dopamine terminals in the living brain using positron emission tomography (PET). To better understand the role of tracer metabolism in dynamic FDOPA PET studies, the pharmacokinetics of individual FDOPA metabolites in extracellular space in the stri...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(00)02649-4
更新日期:2000-09-15 00:00:00
abstract::Current responses to acetylcholine (ACh) and to suberyldicholine (D-6) applied from the double-barrelled ionophoretic micropipette were studied in two identified neurones (LPed-2 and LPed-3) isolated from the left ganglion of pulmonate mollusc, Planorbarius corneus. Experiments made with K2SO4-filled microelectrodes s...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(82)91232-x
更新日期:1982-06-03 00:00:00
abstract::We monitored the protein expression profiles of collapsin response mediator protein 2 and 4 (CRMP2 and CRMP4) throughout cat primary visual area 17 at different postnatal ages. Single immunocytochemical stainings revealed a clear effect of cortical maturation on the spatial and laminar distribution profile of CRMP2 an...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2006.03.028
更新日期:2006-05-09 00:00:00
abstract::We previously described a rat model where the injection of formalin in the tail induced a facilitation of the hindpaw withdrawal reflexes (hyperalgesia). In the present work, after injecting formalin in the tail, we measured the levels of pro-nociceptive mediators tumor necrosis factor-alpha (TNF) and substance P (SP)...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2004.06.007
更新日期:2004-09-03 00:00:00
abstract::The acute intravenous administration of high doses of methylprednisolone sodium succinate (15-90 mg/kg) to cats enhanced (Na+ + K+)-ATPase activity as much as 2-fold in synaptosomes prepared from lumbar spinal cord removed following drug administration. The effect was apparent within 5 min and was statistically signif...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(81)90311-5
更新日期:1981-08-31 00:00:00
abstract::In Long-Evans Cinnamon (LEC) rats of three different ages (7, 13 and 32 weeks old) concentrations of Cu, Zn and Fe were measured in 8 regions of the brain. The LEC groups aged 7 and 13 weeks showed low concentrations of Cu in all regions compared to Long-Evans Agouti (LEA) rats. In 32-week-old LEC rats, however, Cu co...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(92)91587-5
更新日期:1992-08-21 00:00:00
abstract::The light and electron microscopic localization of leucine-enkephalin-containing terminals in the facial nucleus of the rat were investigated by means of the peroxidase-antiperoxidase (PAP) immunocytochemical technique. By light microscopy, leucine-enkephalin-like immunoreactive (LEI) terminals were unevenly distribut...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(83)90517-6
更新日期:1983-09-05 00:00:00
abstract::There have been few reports concerning facilitation and depression in sympathetic ganglia9,17,40. In the present investigation, pairs of excitatory postsynaptic potentials (EPSPs) were recorded intracellularly from bullfrog paravertebral sympathetic ganglia for an analysis of the site and mechanism responsible for the...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(76)90840-4
更新日期:1976-12-10 00:00:00
abstract::Surgical microscopy and electrophysiological techniques were used to standardize the nomenclature for the pudendal nerve and sacral plexus according to their somatic axonal composition in the male rat. We conclude that the pudendal nerve is the segment running from the L6-S1 trunk to the sacral plexus, carrying effere...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(97)00408-3
更新日期:1997-07-25 00:00:00
abstract::Intracellular and extracellular recordings in the arterially-perfused eye cup of pigmented and albino rabbits show the presence of blue cones, green cones and rods. Among the ganglion cells, a fraction shows spectral opponency. Two main types were found. In one, on-depolarizing responses and on-hyperpolarizing respons...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(78)90278-0
更新日期:1978-07-14 00:00:00
abstract::Little is known about the movement of 5-aminolevulinic acid (delta-aminolevulinic acid; ALA) between blood and brain. This is despite the fact that increases in brain ALA may be involved in generating the neuropsychiatric symptoms in porphyrias and that systemic administration of ALA is currently being used to delinea...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(02)03749-6
更新日期:2003-01-10 00:00:00
abstract::The olfactory epithelium is comprised of bipolar sensory neurons, sustentacular cells, and basal cells. The sensory neurons have apical knobs and cilia, which project into the olfactory mucus toward the nasal lumen, and represent presumptive sites of odorant binding. Ionic currents, measured across this epithelium in ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(91)91153-r
更新日期:1991-04-12 00:00:00
abstract::We previously reported that the antinociceptive effect of morphine on the thermal tail withdrawal reflex (tail flick, TF) was reduced in rats that had practiced the TF response prior to morphine administration. This phenomenon, termed behavioral tolerance, was observed even when rats were spinally transected following...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(92)90342-7
更新日期:1992-05-22 00:00:00
abstract::Significant insights into the dynamics of neuronal populations have been gained in the olfactory system where rich spatio-temporal dynamics is observed during, and following, exposure to odours. It is now widely accepted that odour identity is represented in terms of stimulus-specific rate patterning observed in the c...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2011.07.032
更新日期:2012-01-24 00:00:00
abstract::The solubilized rat central nervous system (hypothalamic) nicotinic cholinergic receptor and the Torpedo nicotinic cholinergic receptors are immunologically similar and show greater than 60% immunologic cross-reactivity using a double-antibody technique. Antibodies to the Electrophorus and Torpedo receptors also decre...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(79)90700-5
更新日期:1979-12-14 00:00:00
abstract::There is no sex difference in the number of axodendritic synapses in the lateral septum of adult rats. However, the treatment of adult females with estradiol (E2) for 4 weeks increases significantly the number of synapses, whereas E2 treatment fails to increase the synaptic number in males, suggesting a possible sexua...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(87)91574-5
更新日期:1987-12-08 00:00:00
abstract::Intracerebroventricular (ICV) streptozotocin (STZ) treated rat has been described as a suitable model for sporadic Alzheimer's disease (AD). Central application of STZ has demonstrated behavioral and neurochemical features that resembled those found in human AD. Chronic treatments with antioxidants, acetylcholinestera...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2011.09.056
更新日期:2011-12-02 00:00:00
abstract::Recent immunohistochemical studies have suggested that the forebrain distribution of stigmoid bodies, marked by an antibody against placental aromatase-associated antigen X-P2 (PAX), overlaps with that of the common binding sites of androgen and estrogen. In the present light- and electron-microscopy study the coexist...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(94)91933-x
更新日期:1994-01-21 00:00:00
abstract::A procedure is described which allows for the selective and non-injectional staining of the three-dimensional microvasculatory bed (MVB) in thick sections (60-140 microm) of formalin-fixed brain tissue of white rats Rattus norvegicus. This histochemical method detects ATPase activity and takes place between pH 10.5 an...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2005.11.059
更新日期:2006-01-27 00:00:00
abstract::We have shown that, when an amyloid-beta peptide (Abeta) overproducing transgenic mouse model (PSAPP) of Alzheimer's disease (AD) is treated with a depleting antibody against CD40L, it causes marked attenuation of Abeta pathology associated with decreased amyloidogenic processing of amyloid precursor protein (APP) and...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2004.05.004
更新日期:2004-07-23 00:00:00
abstract::Nestin is an embryonic intermediate filament that transiently expressed in the neural stem/progenitor cells in the developing central nervous system (CNS). Growing evidence has shown that abundant expression of nestin also occurs in both pathological glial-derived tumor cells and reactive astrocytes in various CNS inj...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2007.11.026
更新日期:2008-02-27 00:00:00
abstract::Isolated neuronal cells dissociated from the brain of embryonic rabbits on the sixteenth day of gestation and of perinatal rats (eighteenth embryonic day, to E18, thirteenth day postnatum, p.n. 13) were selectively cultured using a plasma clot technique. The cells grown were shown to be neurons by means of the neuron-...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0165-3806(83)90179-7
更新日期:1983-04-01 00:00:00
abstract::We compared the relative abilities of a series of calmodulin inhibitors and protein kinase C inhibitors to influence 45Ca2+ influx through voltage-dependent Ca2+ channels in PC12, a clonal neural cell line. K+-depolarization-dependent 45Ca2+ uptake was reduced by the calmodulin inhibitors calmidazolium, trifluoperazin...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(87)91403-x
更新日期:1987-02-24 00:00:00
abstract::The present study examines the effects of ibotenic acid lesions of the medial amygdala, the bed nucleus of the stria terminalis and the medial preoptic area on the display of paced mating behavior in female rats. Lesions of either the medial amygdala or the bed nucleus of the stria terminalis have no effect on the dis...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2003.10.056
更新日期:2004-02-27 00:00:00