Abstract:
:Isolated neuronal cells dissociated from the brain of embryonic rabbits on the sixteenth day of gestation and of perinatal rats (eighteenth embryonic day, to E18, thirteenth day postnatum, p.n. 13) were selectively cultured using a plasma clot technique. The cells grown were shown to be neurons by means of the neuron-specific synaptosomal plasma membrane antibody (SPM). They differentiated at a very high frequency from rounded cells lacking processes into different shapes characteristic for several neuronal cell types. Morphological differences could be distinguished even after 24 h in culture. The neurons differentiated in vitro for up to 11 days, apparently without need of any direct intercellular contact. Cells caught inside the plasma clot were prevented from decreasing in number. This provides the opportunity to culture few neurons even from an extremely small area of a single brain. As an example, different cell types are shown originating from rat cerebella aged E18 to p.n. 13. Their appearance apparently corresponds to the genesis of cerebellar cell types, as is known from the in vivo situation. The high degree of characteristic neuronal differentiation and the prevention of direct intercellular contacts indicate that this culture method may serve as an in vitro assay for genetically fixed properties acquired in vivo.
journal_name
Brain Resjournal_title
Brain researchauthors
Buse E,Matthaei Hdoi
10.1016/0165-3806(83)90179-7subject
Has Abstractpub_date
1983-04-01 00:00:00pages
221-34issue
2-3eissn
0006-8993issn
1872-6240journal_volume
283pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract::Non-peptide receptor ligands with differential affinity for the angiotensin II-1 (AII-1) receptor (EXP3312, EXP3880) or the AII-2 receptor (PD123177) and an angiotensin converting enzyme (ACE) inhibitor captopril were evaluated for the ability to protect against a renin-induced performance deficit in a passive avoidan...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(91)91599-v
更新日期:1991-10-11 00:00:00
abstract::Previous findings from our laboratory demonstrated [125I]angiotensin II (AII) binding to plasma membranes from rat but not gerbil circumventricular organs (CVOs), the presumed location of brain receptors for angiotensin-induced dipsogenicity. Since members of both species drink to intracranially applied AII, a degrada...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(84)90822-9
更新日期:1984-03-12 00:00:00
abstract::The effects of infusing N-methyl-d-aspartate (NMDA) into the raphe nuclei on release of 5-HT in this brain region and also the frontal cortex of the same animal were studied using in vivo microdialysis in freely moving rats. Infusion of 25 microM NMDA into the raphe led to a substantial decrease in dialysate 5-HT in t...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(97)01333-4
更新日期:1998-02-09 00:00:00
abstract::Nicergoline, a drug used for the treatment of Alzheimer's disease and other types of dementia, was tested for its ability to protect neurons against beta-amyloid toxicity. Pure cultures of rat cortical neurons were challenged with a toxic fragment of beta-amyloid peptide (betaAP(25-35)) and toxicity was assessed after...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2005.04.004
更新日期:2005-06-14 00:00:00
abstract::It has been suggested that individual predisposition to develop amphetamine self-administration is associated with impairment in corticosteroid negative feedback mechanisms. Since corticosteroid receptors, particularly those in the hippocampus, are involved in corticosterone feedback sensitivity, we examined the relat...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(91)91137-p
更新日期:1991-05-10 00:00:00
abstract::Dexmedetomidine (Dex) has the neuroprotective effect on cerebral ischemia-reperfusion injury (CIRI). But the mechanism is not yet clear. In this study, we established a model of middle cerebral artery occlusion (MCAO) and treated primary cortical neurons with oxygen glucose deprivation (OGD), followed by Dex treatment...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2018.09.007
更新日期:2018-12-15 00:00:00
abstract::Opioid modulation of calcium currents was studied in acutely dissociated rat basal forebrain neurons using the whole cell patch-clamp recording technique. The mu-opioid receptor agonist DAGO reversibly suppressed high-voltage activated calcium currents and slowed their rate of activation, while neither delta- nor kapp...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(97)00206-0
更新日期:1997-05-30 00:00:00
abstract::Previous studies have suggested that monosynaptic transmission between spinal primary afferent fibers and motoneurons is mediated by an excitatory amino acid, most likely glutamate or aspartate. No such comparable studies have been carried out in the trigeminal system. In an attempt to elucidate the neurotransmitter(s...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(89)91413-3
更新日期:1989-01-16 00:00:00
abstract::Repeated electrode penetration of the dentate and interpositus nuclei in a rhesus monkey transformed an 11-13 Hz physiologic tremor into a much larger action tremor at 5-7 Hz. This tremor was associated with muscle spindle spike train modulation and reflexly evoked tremor modulation of interpositus and motor cortex ne...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(84)90308-1
更新日期:1984-12-10 00:00:00
abstract::A central component of Attention Deficit Hyperactivity Disorder (ADHD) is increased distractibility in response to visual and auditory stimuli, which is linked to the superior colliculus (SC). Furthermore, there is now mounting evidence of altered collicular functioning in ADHD and it is proposed that a hyper-responsi...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2015.09.038
更新日期:2015-12-10 00:00:00
abstract::A search for vasomotor depressor sites in the lateral medullary brainstem of anesthetized cat using microinjections of AMPA has delineated a longitudinal column of sympathoinhibitory neurons consisting of the rostral depressor area, the caudal ventrolateral medulla, and a new intermediate depressor area. Systematic bl...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(93)91257-s
更新日期:1993-02-19 00:00:00
abstract::The intracellular localization of the serine protease plasminogen activator was analyzed in homogenates of bovine brain cortex using differential fractionation procedures. The distribution of the enzyme was clearly different from that of cytosol and mitochondrial markers, and was similar to that of plasma membrane pro...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(82)91154-4
更新日期:1982-09-23 00:00:00
abstract::Spontaneous and hypothalamically induced hippocampal rhythmical slow activity (RSA or theta) was studied acutely in rats anaesthetized with urethane or immobilized with D-tubocurarine. Systematic tracking of microelectrodes showed two foci of hippocampal RSA, one located in the basal part (stratum oriens) of CAl (mean...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(76)90711-3
更新日期:1976-12-17 00:00:00
abstract::By means of the [14C]deoxyglucose method the local cerebral glucose utilization (LCGU) was measured in the lateral geniculate nucleus and the superior colliculus of rats with a simultaneous double enucleation, animals with an enucleation of the right eye between neonatal and adult stages followed by an enucleation of ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(89)90688-4
更新日期:1989-05-29 00:00:00
abstract::The distribution of degeneration in the vestibular nuclei (VN) has been studied in transversely cut sections from 9 cats with stereotaxically performed lesions in the main reticular formation (RF) of the brain stem (Nauta of Fink and Heimer method). No projection was found from the mesencephalic reticular formation (R...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(75)90224-3
更新日期:1975-09-05 00:00:00
abstract::In kindled rats, the administration of cysteamine (CSH, 200 mg/kg, i.p.) 4 h prior to a kindled seizure leads to long-term (up to 10 days) inhibition of kindled seizures. CSH (200 mg/kg, i.p.) also induces myoclonic seizures in kindled rats. We suggest that the long-term inhibition of kindled seizures might be the res...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(87)91453-3
更新日期:1987-05-26 00:00:00
abstract::Neuropeptide Y (NPY) blocks the effect of light on the mammalian circadian clock during the subjective night. The present study explores the role of the NPY Y1 receptor in this interaction. The effect of NPY when co-applied with NMDA, a glutamate agonist that can mimic the effect of light, was examined in NPY Y1-/- mi...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2004.07.037
更新日期:2004-10-08 00:00:00
abstract::Obesity and related metabolic disorders are important side effects of some antipsychotic drugs (APs). The currently available animal model of AP-induced bodyweight gain (BWG) in rats is based on administration of sulpiride (SUL). However, this model has important limitations. For example, SUL is a pure dopamine antago...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(02)03616-8
更新日期:2002-12-06 00:00:00
abstract::Cortistatin (CST) is a recently described neuropeptide with high structural homology with somatostatin. Its mRNA is restricted to gamma amino butyric acid (GABA)-containing cells in the cerebral cortex and hippocampus. CST modulates the electrophysiology of the hippocampus and cerebral cortex of rats; hence, it may be...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(99)02336-7
更新日期:2000-03-06 00:00:00
abstract::Application of 4-aminopyridine (4-AP) has previously been reported to produce different patterns of epileptiform discharges in entorhinal cortex (EC)-hippocampal slices: recurrent short discharges (RSDs) in hippocampal area CA1, seizure-like events (SLEs) and negative-going potentials (NGPs) in the medial entorhinal c...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(99)02348-3
更新日期:2000-03-17 00:00:00
abstract::Recent studies have demonstrated that sensory hair cells in the avian inner ear are reproduced by cell proliferation in response to the death of the original hair cell population. The regenerated hair cells appear to construct functional synaptic contacts, thereby transmitting acoustic signals to the peripheral nervou...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(95)01467-5
更新日期:1996-05-13 00:00:00
abstract::The type and cell size of parvalbumin-immunoreactive (PV-Ir) neurones were examined in 14 postmortem brains from elderly control and Alzheimer-type dementia (ATD) patients with the aid of an image analyser. Morphological features of PV-Ir neurones suggested the existence of PV in the non-pyramidal interneurones in the...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(87)90974-7
更新日期:1987-08-18 00:00:00
abstract::Studies of various strains of mice revealed marked differences in their analgesic sensitivity towards morphine (mu), U50,488H (kappa 1) and naloxone benzoylhydrazone (NalBzoH; kappa 3). Sensitivity to mu and kappa analgesia varied independently of the other. Analgesic sensitivity to morphine remained relatively consis...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(91)91712-a
更新日期:1991-12-06 00:00:00
abstract::We reported earlier that overnight change in explicit memory is positively related to the change in sleep spindle activity (between a control and a learning night). However, it remained unclear whether this effect was restricted to good memory performers and whether a general association of sleep spindles and a "sleep...
journal_title:Brain research
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1016/j.brainres.2007.10.106
更新日期:2008-01-29 00:00:00
abstract::We report an fMRI experiment on deductive reasoning with acoustically presented problems. Twelve volunteers received problems in which an acoustic stimulus came from the left or the right of another stimulus. The participants then heard a third stimulus coming from the left or the right of one of the proceeding stimul...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/j.brainres.2008.10.025
更新日期:2009-01-16 00:00:00
abstract::In the sea hare, Aplysia californica, consummatory feeding behavior is selectively abolished by bilateral crushes of the cerebral-buccal connectives and recovers by postlesion day 13. Recovered biting responses are initially weak and increase in magnitude gradually with time. The lesions do not affect appetitive feedi...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(92)91217-3
更新日期:1992-07-10 00:00:00
abstract::The effects of selective dopamine D1 (SKF 38393) and D2 (4-propyl-9-hydroxynaphthoxazine or PHNO) agonists on free-running rhythms of locomotor activity in rats were investigated under either constant light (LL) or dark (DD) condition. Rats under the DD condition exhibited clear circadian patterns of activity, while c...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/0006-8993(91)90445-2
更新日期:1991-01-11 00:00:00
abstract::Ion-selective microelectrode techniques were used to study extracellular K+ changes associated with penicillin-induced epileptogenesis in the CA3 region of hippocampal slices. Recordings were made in slices taken from rats 9-16 days of age, which have a pronounced capacity to undergo prolonged synchronized afterdischa...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(86)80203-7
更新日期:1986-12-01 00:00:00
abstract::The occurrence of hypoxia-ischemia (HI) during early fetal or neonatal stages of an individual leads to the damaging of immature neurons resulting in behavioral and psychological dysfunctions, such as motor or learning disabilities, cerebral palsy, epilepsy or even death. No effective treatment is currently available ...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(02)03094-9
更新日期:2002-09-27 00:00:00
abstract::To achieve a better understanding of the parallel information processing that takes place in the nervous system, many researchers have recently begun to use multielectrode techniques to obtain high spatial- and temporal-resolution recordings of the firing patterns of neural ensembles. Apart from the complexities of ac...
journal_title:Brain research
pub_type: 杂志文章
doi:10.1016/s0006-8993(00)03072-9
更新日期:2000-12-22 00:00:00