Demonstration of phosphatidylinositol anchors on Ly-6 molecules by specific phospholipase C digestion and gel electrophoresis in octylglucoside.

abstract：:The kallikrein family (KLK) has been implicated in cancer and may be useful as tumor markers. Here, we compared the 15 KLK genes' expression in malignant and normal breast tissues using real-time quantitative PCR. Most KLKs were expressed at lower levels in breast cancer compared to normal breast tissue. The only exce...

journal_title：Biochemical and biophysical research communications

authors： Mangé A,Desmetz C,Berthes ML,Maudelonde T,Solassol J

更新日期：2008-10-10 00:00:00

abstract：:Hypothesis of non-enzymatic recognition of primordial tRNA and mRNA precursors is experimentally approached. DNA hairpins containing a different number of deoxyguanosine residues in the loop are analyzed for their binding ability to a chemically fixed single-strand of oligo(dC). In presence of small Mg2+ concentration...

journal_title：Biochemical and biophysical research communications

authors： Baumann U,Frank R,Blöcker H

更新日期：1988-12-30 00:00:00

abstract：:GLM, a luteolin derivative, shows anti-melanogenic effect via regulation of various signal molecules; however, it is unclear whether it also exerts anti-inflammatory effect. This study investigated the mechanisms of the anti-inflammatory effect of GLM on activated dendritic cells (DCs) to elucidate its therapeutic pot...

journal_title：Biochemical and biophysical research communications

authors： Kim WS,Song HY,Han JM,Byun EB

更新日期：2019-10-08 00:00:00

abstract：:RANTES is a potent chemotactic and activating agent for a variety of leukocytes including T-lymphocytes. To identify the region of the molecule responsible for this activity, we have made overlapping 10 amino acid peptides scanning the protein. A micro-Boyden chamber chemotaxis assay showed the most efficacious peptid...

journal_title：Biochemical and biophysical research communications

authors： Wells TN,Guye-Coulin F,Bacon KB

更新日期：1995-06-06 00:00:00

abstract：:T box (Tbx) genes are a large family of transcription regulators that play critical roles in invertebrate and vertebrate development. Mutations in Tbx5 gene have been found to cause Holt-Oram syndrome (HOS) in humans. Partial dysfunction of TBX5 in mouse also causes HOS phenotype. Little is known about its molecular a...

journal_title：Biochemical and biophysical research communications

authors： He ML,Chen Y,Peng Y,Jin D,Du D,Wu J,Lu P,Lin MC,Kung HF

更新日期：2002-09-20 00:00:00

abstract：:We examined voltage-dependent, TTX-resistant Na+ channels of newt retina (nRNaCh) electrophysiologically. IC50-TTX value of nRNaCh is more than 20 microM. We determined partial cDNA sequences of nRNaCh restricted to TTX binding sites (SS2 regions of all four repeats). While the amino acid sequences of SS2 regions of r...

journal_title：Biochemical and biophysical research communications

authors： Kaneko Y,Matsumoto G,Hanyu Y

更新日期：1997-11-26 00:00:00

abstract：:The effect of tributyltin (TBT) on human intestinal epithelial cell functions was investigated by using human intestinal Caco-2 cell monolayers. We paid particular attention to the effect of TBT on two barrier functions: the tight junction as a physical barrier and MDR1/P-glycoprotein as a biological barrier. A loss o...

journal_title：Biochemical and biophysical research communications

authors： Tsukazaki M,Satsu H,Mori A,Sugita-Konishi Y,Shimizu M

更新日期：2004-03-19 00:00:00

abstract：:Phytoplankton and zooplankton serve as major food material for primary and secondary consumers in the marine food web. Although phytoplankton are primary targets to UV radiation damaging effects, we have demonstrated that stratospheric ozone depletion will affect zooplankton to contribute to inhibition in biomass prod...

journal_title：Biochemical and biophysical research communications

authors： Soni AK,Joshi PC

更新日期：1997-02-24 00:00:00

abstract：:Mutual interactions between adipocytes and immune cells in white adipose tissue (WAT) are involved in modulation of lipid metabolism in the tissue and also in response to omega-3 polyunsaturated fatty acids (PUFA), which counteract adverse effects of obesity. This complex interplay depends in part on in situ formed an...

journal_title：Biochemical and biophysical research communications

authors： Kuda O,Rombaldova M,Janovska P,Flachs P,Kopecky J

更新日期：2016-01-15 00:00:00

abstract：:Although major depressive disorder (MDD) has caused severe mental harm to overwhelming amounts of patients, the pathogenesis of MDD remains to be studied. Due to the in-depth discussion of the mechanism of new antidepressants like ketamine, the habenula (Hb) was reported to be significant in the onset of MDD and the a...

journal_title：Biochemical and biophysical research communications

authors： Tian Y,Wu Z,Wang Y,Chen C,He Y,Lan T,Li Y,Bai M,Yu H,Chen X,Chen Z,Cheng K,Xie P

更新日期：2021-01-01 00:00:00

abstract：:Protocols were defined that enable patch-clamp studies of the approximately 107 pS voltage dependent channel and a class of activity we refer to as MCC (multiconductance channel) which is characterized by multiple levels and transitions as high as 1 to 1.5 nS. If free calcium was kept at 10(-7) M or lower during mitoc...

journal_title：Biochemical and biophysical research communications

authors： Kinnally KW,Zorov D,Antonenko Y,Perini S

更新日期：1991-05-15 00:00:00

abstract：:Epinephrine was used to activate the heparin non-releasable lipoprotein lipase (LPL) in the 3 skeletal muscle fiber types of the perfused rat hindlimb. Following a 9 min washout of the capillary-bound lipoprotein lipase, the hindquarter of the rat was perfused with a buffer containing 10 nM of epinephrine. Activity of...

journal_title：Biochemical and biophysical research communications

authors： Miller WC,Gorski J,Oscai LB,Palmer WK

更新日期：1989-10-31 00:00:00

abstract：:Hypothermia followed by slow rewarming is neuroprotective for ischemic stroke. However, slow rewarming causes patients' longer stay in intensive care unit and increases the risk of hypothermic complications. Hypothermia followed by rapid rewarming (HTRR) is more convenient; but it exacerbates intracranial hypertension...

journal_title：Biochemical and biophysical research communications

authors： Zhu SZ,Gu Y,Wu Z,Hu YF,Pan SY

更新日期：2016-05-20 00:00:00

abstract：:We investigated the effect of chronic alcohol ingestion on buccal mucosal ulcer healing by analyzing the interplay between mucosal expression of tumor necrosis factor-alpha (TNF-alpha), endothelin-1 (ET-1), and interleukin-4 (IL-4). Chronic ulceration was induced in rats maintained for 5 weeks on alcohol-containing or...

journal_title：Biochemical and biophysical research communications

authors： Slomiany BL,Piotrowski J,Slomiany A

更新日期：1999-04-13 00:00:00

abstract：:A fast and robust assay, based on the combination of the highly sensitive immuno-PCR (IPCR), employing standardized self-assembled DNA-protein conjugates as reagents, and the well-established, reliable, and fast real-time PCR detection by means of the TaqMan principle is introduced in this work. The use of anti-specie...

journal_title：Biochemical and biophysical research communications

authors： Adler M,Wacker R,Niemeyer CM

更新日期：2003-08-22 00:00:00

abstract：:A sample of highly oriented Na-hyaluronate was prepared with a wet--spinning method and equilibrated to 75% relative humidity. Its proton NMR spectrum was recorded as a function of the angle theta between the hyaluronate helix axis and the external magnetic field. It appeared as a singlet with an angle-dependent line ...

journal_title：Biochemical and biophysical research communications

authors： Lahajnar G,Rupprecht A

更新日期：1986-11-26 00:00:00

abstract：:Using a monospecific antibody to the major cytosolic glutathione-S-transferase of human liver, we have isolated a cDNA clone from a human liver cDNA expression vector library in lambda gt11. The clone cross-hybridizes with a rat liver ligandin (glutathione-S-transferase 1-2) cDNA probe. The clone has an insert of 1.25...

journal_title：Biochemical and biophysical research communications

authors： Butera L,Monnier JR,Campbell E,Bhargava MM

更新日期：1987-02-13 00:00:00

abstract：:Phospholipase C (PLC) has been known to be a key effector protein in signal transduction pathway for cell proliferation and differentiation. Here, we report the transcriptional regulation of PLC-gamma 1 during myogenic differentiation. Both PLC-gamma 1 mRNA and protein levels were increased during myogenesis. The acti...

journal_title：Biochemical and biophysical research communications

authors： Lee SJ,Lee YH,Kim YS,Ryu SH,Suh PG

更新日期：1995-01-05 00:00:00

abstract：:Bacterial dichloromethane dehalogenases catalyze the glutathione-dependent hydrolysis of dichloromethane to formaldehyde and are members of the enzyme superfamily of glutathione S-transferases involved in the detoxification of electrophilic compounds. Numerous protein engineering studies have addressed questions perta...

journal_title：Biochemical and biophysical research communications

authors： Vuilleumier S,Sorribas H,Leisinger T

更新日期：1997-09-18 00:00:00

abstract：:High affinity interactions between cells and laminin are mediated, at least in part, by the 67 kDa laminin receptor (67 LR). A 37 kDa nascent polypeptide (37 LRP), predicted by a full length cDNA clone and obtained by in vitro translation of hybrid-selected laminin receptor mRNA, has been immunologically identified in...

journal_title：Biochemical and biophysical research communications

authors： Castronovo V,Claysmith AP,Barker KT,Cioce V,Krutzsch HC,Sobel ME

更新日期：1991-05-31 00:00:00

abstract：:We tested usefulness of a target enrichment system SureSelect, a comprehensive viral nucleic acid detection method, for rapid identification of viral pathogens in feces samples of cattle, pigs and goats. This system enriches nucleic acids of target viruses in clinical/field samples by using a library of biotinylated R...

journal_title：Biochemical and biophysical research communications

authors： Oba M,Tsuchiaka S,Omatsu T,Katayama Y,Otomaru K,Hirata T,Aoki H,Murata Y,Makino S,Nagai M,Mizutani T

更新日期：2018-01-08 00:00:00

abstract：:Chloroperoxidase was present as Compound II during the peroxidatic oxidation of ascorbic acid. Compound III (oxy-form) was formed when excess hydrogen peroxide was added to Compound II. By decreasing the temperature it was possible to measure the spectra of Compounds II and III in the Soret and visible regions. Each s...

journal_title：Biochemical and biophysical research communications

authors： Nakajima R,Yamazaki I,Griffin BW

更新日期：1985-04-16 00:00:00

abstract：:Cells from patients with acute myeloid leukaemia (AML) contain an abnormally high UDP-GlcNAc: Gal beta 1-3GalNAc-R (GlcNAc to GalNAc) beta 6-N-acetylglucosaminyltransferase (core 2 beta 6-Gn-T) activity. Upon UV irradiation at 350 nm, the substrate Gal beta 1-3GalNAc alpha-p-nitrophenyl acted as an effective inhibitor...

journal_title：Biochemical and biophysical research communications

authors： Toki D,Granovsky MA,Reck F,Kuhns W,Baker MA,Matta KL,Brockhausen I

更新日期：1994-01-28 00:00:00

abstract：:For a series of adenosine analogues a close correlation (r = 0.986) was found between their antiviral potency (against vesicular stomatitis virus) and their inhibitory effects (Ki/Km) on S-adenosylhomocysteine (AdoHcy) hydrolase; thus, in order of increasing inhibitory potency for both virus replication and AdoHcy hyd...

journal_title：Biochemical and biophysical research communications

authors： De Clercq E,Cools M

更新日期：1985-05-31 00:00:00

abstract：:It is well known that some intestinal bacteria, such as Escherichia coli, can produce a remarkable amount of molecular hydrogen (H(2)). Although the antioxidant effects of H(2) are well documented, the present study examined whether H(2) released from intestinally colonized bacteria could affect Concanavalin A (ConA)-...

journal_title：Biochemical and biophysical research communications

authors： Kajiya M,Sato K,Silva MJ,Ouhara K,Do PM,Shanmugam KT,Kawai T

更新日期：2009-08-21 00:00:00

abstract：:AMP, an allosteric inhibitor at neutral pH, activates snakes muscle fructose 1,6-bisphosphatase at pH 9.2. The activation is virtually unique for the snake muscle enzyme: activation was not observed for the enzymes from either human and rabbit liver or porcine kidney. The activation is Mg(2+)-dependent but was not obs...

journal_title：Biochemical and biophysical research communications

authors： Zhao FK,Xu SQ,Xu GJ

更新日期：1998-03-27 00:00:00

abstract：:DNA methylation plays a central role in the epigenetic regulation of gene expression during development and progression of cancer diseases. The inheritance of specific DNA methylation patterns are acquired in the early embryo and are specifically maintained after cellular replication via the DNA methyltransferase 1 (D...

journal_title：Biochemical and biophysical research communications

authors： Lavoie G,St-Pierre Y

更新日期：2011-06-03 00:00:00

abstract：:Imidazoquinoline compounds, such as resiquimod (R-848), are well known topically active immune modifiers that bind to toll-like receptor 7 (TLR7). The aim of this study was to characterize the R-848 induced inflammatory response in mice and to validate the response using methyl-prednisolone and anti-TNF antibody. Intr...

journal_title：Biochemical and biophysical research communications

authors： Karlsson A,Jägervall K,Utkovic H,Karlsson L,Rehnström E,Fredin MF,Gillberg PG,Jansson L,Michaëlsson E,Melgar S

更新日期：2008-03-07 00:00:00

abstract：:Ezetimibe is a cholesterol-lowering agent targeting Niemann-Pick C1-like 1, an intestinal cholesterol transporter. Inhibition of intestinal cholesterol absorption with ezetimibe may ameliorate several metabolic disorders including hepatic steatosis and insulin resistance. In this study, we investigated whether chronic...

journal_title：Biochemical and biophysical research communications

authors： Yang SJ,Choi JM,Kim L,Kim BJ,Sohn JH,Kim WJ,Park SE,Rhee EJ,Lee WY,Oh KW,Park SW,Kim SW,Park CY

更新日期：2011-04-01 00:00:00

abstract：:Dexamethasone (DEX), a glucocorticoid which induces cleft palate, causes marked alterations in the synthesis and degradation of phosphatidylinositol (PI) but not phosphatidylcholine in an established fibroblastic cell line derived from a human embryonic palate. Incorporation of radiolabeled inositol into phosphatidyli...

journal_title：Biochemical and biophysical research communications

authors： Grove RI,Willis WD,Pratt RM

更新日期：1983-01-14 00:00:00