Calcium modulation of mitochondrial inner membrane channel activity.

Abstract:

:Protocols were defined that enable patch-clamp studies of the approximately 107 pS voltage dependent channel and a class of activity we refer to as MCC (multiconductance channel) which is characterized by multiple levels and transitions as high as 1 to 1.5 nS. If free calcium was kept at 10(-7) M or lower during mitochondrial isolation, no activity was observed at low voltage (+/- 60 mV). If free calcium levels were higher, MCC activity was observed in about 96% of the patches. The observation of approximately 107 pS channel was enhanced from 2% to 68% of patches by washing isolated mitoplasts (mitochondria stripped of outer membrane) with EGTA. Increasing matrix calcium from 10(-9) to 10(-5) M decreased the probability of opening for the MCC and approximately 107 pS activities.

authors

Kinnally KW,Zorov D,Antonenko Y,Perini S

doi

10.1016/0006-291x(91)90410-9

subject

Has Abstract

pub_date

1991-05-15 00:00:00

pages

1183-8

issue

3

eissn

0006-291X

issn

1090-2104

pii

0006-291X(91)90410-9

journal_volume

176

pub_type

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