Abstract:
:Recent studies by several research groups have shown that G protein estrogen receptor-1 (GPER) formerly known as GPR30, mediates 17beta-estradiol (E2) activation of signal transduction pathways in a variety of human cancer cells and displays E2 binding typical of a membrane estrogen receptor. However, the importance of GPER as an estrogen receptor has been questioned by Otto and co-workers. Some of the pitfalls in investigating the functions of recombinant steroid membrane receptors that may explain the negative results of these investigators are discussed. The characteristics of GPER have also been investigated in a teleost fish, Atlantic croaker, where it has been shown to mediate E2 inhibition of oocyte maturation. Investigations on newly discovered homologous proteins from distantly related vertebrate groups are valuable for determining their fundamental, evolutionarily conserved functions. Therefore, the functions of croaker and human GPERs were compared. The comparisons show that croaker and human GPER have very similar estrogen binding characteristics, typical of estrogen membrane receptors, and activate the same estrogen signaling pathways via stimulatory G proteins (Gs) resulting in increased cAMP production. These results suggest that the estrogen binding and estrogen signaling functions of GPER arose early in vertebrate evolution, prior to the divergence of the teleosts from the tetrapods, more than 200 million years ago. The finding that estrogen membrane signaling through GPER has been conserved for such a long period in two distantly related vertebrate groups, mammals and fish, suggests that this is a fundamental function of GPER in vertebrates, and likely its major physiological role.
journal_name
Steroidsjournal_title
Steroidsauthors
Thomas P,Alyea R,Pang Y,Peyton C,Dong J,Berg AHdoi
10.1016/j.steroids.2009.11.005subject
Has Abstractpub_date
2010-08-01 00:00:00pages
595-602issue
8-9eissn
0039-128Xissn
1878-5867pii
S0039-128X(09)00250-5journal_volume
75pub_type
杂志文章,评审相关文献
STEROIDS文献大全abstract::Parathyroid hormone-related protein (PTHrP) increases the growth and osteolytic potential of prostate cancer cells, making it important to control PTHrP expression in these cells. We show that 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) and its non-hypercalcemic analog, EB1089, decrease PTHrP mRNA and cellular protein leve...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2005.08.009
更新日期:2006-02-01 00:00:00
abstract::The luteinizing hormone (LH) surge initiates a cascade of proteolytic events that control ovulation. One of the genes induced by LH is the progesterone receptor (PR). Because mice with a mutant PR gene (PRKO) fail to ovulate and are infertile, we have used them as a model in which to determine PR target genes that mig...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(00)00114-8
更新日期:2000-10-01 00:00:00
abstract::Steroid delta 4-5 alpha- and delta 4-5 beta-reductase activity was determined in 16 human mammary tumors and 8 DMBA-induced rat mammary tumors using a spectrophotometric assay. Steroid delta 4-5 alpha-reductase was present in all tumors investigated while delta 4-5 beta-reductase was detected in only 6 estrogen recept...
journal_title:Steroids
pub_type: 历史文章,杂志文章
doi:10.1016/s0039-128x(79)80010-0
更新日期:1979-01-01 00:00:00
abstract::Contraceptive methods for breastfeeding women should be safe for the mother and infant and should not interfere with lactation. Progestin-only methods meet these conditions and can be used from the sixth week postpartum. Because all progestins are excreted in milk, those that are insufficiently active by the oral rout...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(00)00125-2
更新日期:2000-10-01 00:00:00
abstract::Three BS-BODIPY (brassinosteroids-4,4-difluoro-4-bora-3a,4a-diaza-s-indacene) conjugates were synthesized and their fluorescent and immunological properties were investigated. Two of the conjugates, having present all the functional groups characteristic of BS, were shown to be potentially useful as biological probes ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2015.07.002
更新日期:2015-10-01 00:00:00
abstract::Two unnatural steroid sapogenins bearing a furospirostane side chain were prepared starting from the readily available spirostane sapogenins, tigogenin and diosgenin following a synthetic protocol that included: (i) introduction of a carbonyl group at position C-23, (ii) diacetoxyiodobenzene-induced F-ring contraction...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2013.05.013
更新日期:2013-09-01 00:00:00
abstract::Progesterone and its polar metabolite(s) trigger the meiotic divisions in the amphibian oocyte through a non-genomic signaling system at the plasma membrane. Published site-directed mutagenesis studies of ouabain binding and progesterone-ouabain competition studies indicate that progesterone binds to a 23 amino acid e...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2012.04.012
更新日期:2012-09-01 00:00:00
abstract::Brassinosteroids (BRs) are an important class of plant hormones with a multitude of functions. They have been intensively investigated for their biosynthesis, distribution and physiological functions. The aim of this study was to examine possible effects of BRs on the plant plasma membrane cation conductances and Ca(2...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2014.10.008
更新日期:2015-05-01 00:00:00
abstract::Saturation analysis of the binding of [3H]dexamethasone [( 3H]DEX) to ammonium sulfate precipitates (ASPs) confirmed the presence of a limited-capacity, high-affinity binder in human adipose tissue cytosols. Various non-radioactive steroids competed with [3H]DEX for binding to the ASPs in the following sequence: dexam...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(87)90091-2
更新日期:1987-06-01 00:00:00
abstract::Gypensapogenin H (GH) is a novel dammarane-type triterpenes obtained from hydrolyzate of total saponins from Gynostemma pentaphyllum and its anti-tumor activity has been studied in previous work. In this study, we report the effects of this compound on human prostate cancer cells (DU145 and 22RV-1). It significantly i...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2015.10.014
更新日期:2015-12-01 00:00:00
abstract::A simplified, non-chromatographic method for aldosterone-gamma-lactone (2) radio-immunoassay is described, using a high titer aldosterone-gamma-lactone antibody. This method takes six working hours for completion; the blanks are below the sensitivity of the assay (0-10 pg), and the intra and interassay coefficient of ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(76)90004-0
更新日期:1976-11-01 00:00:00
abstract:BACKGROUND:Urinary steroid profiling by GC or GC-MS are established clinical tools to complement other biochemical tests in the diagnosis and investigation of a wide range of adrenocortical disorders, but normative data on adults using the more specific GC-MS are lacking. Our objective was to set up the reference inter...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2008.03.004
更新日期:2008-09-01 00:00:00
abstract::Radioactive mestranol (ME) and/or ethynylestradiol (EE) were administered to women in Nigeria, Sri Lanka, and the USA, and the types and patterns of radioactive urinary conjugates examined by Sephadex LH-20 chromatography. There are no differences in the total excretion of urinary radioactivity over 3 days. Consistent...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(80)90001-x
更新日期:1980-09-01 00:00:00
abstract::Human placental 17 beta, 20 alpha-hydroxysteroid dehydrogenase was completely inactivated by the affinity alkylator, 3-bromoacetoxy-1,3,5(10)-estratrien-17-one (estrone 3-bromoacetate). The inactivated enzyme was then reactivated to 100% of the enzyme activity by base-catalyzed hydrolysis of the steroidalester-enzyme ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(84)90039-4
更新日期:1984-02-01 00:00:00
abstract::By the conventional methods of gas liquid chromatography (GLC) as well as mass spectrometry, 3beta,7alpha-dihydroxychol-5-en-24-oic acid (delta5-acid), a key intermediate of chenodeoxycholic acid biogenesis and its metabolic by-product, 3alpha,7alpha-dihydroxychol-4-en-24-oic acid (delta4-acid) have not yet been ident...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(78)90102-2
更新日期:1978-07-01 00:00:00
abstract::Efforts to develop a novel class of nonsteroidal aromatase inhibitors began with the discovery that the infertility in male rats exposed to high levels of the agricultural fungicide, fenarimol (alpha-(2-chlorophenyl)-alpha-(4-chlorophenyl)-5-pyrimidine-methanol), was attributable to the inhibition of aromatase activit...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(83)90072-7
更新日期:1987-07-01 00:00:00
abstract::An enzyme immunoassay for estrone in swine serum was established. For this, beta-galactosidase from E. coli was conjugated through estrone-17 (O-carboxymethyl)oxime using a mixed anhydride reaction. The percentage of immunoreactive estrone-17 (O-carboxymethyl)oxime-beta-galactosidase conjugate was estimated to be abou...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(82)90136-2
更新日期:1982-06-01 00:00:00
abstract::Estrogen receptor (ER) positive primary breast cancers have a wide range of clinical outcomes. Prediction of the likely course of the disease aids treatment decision-making. In the translational arm of the ATAC (anastrozole or tamoxifen alone or combined) trial (TransATAC) we have assessed individual and multiparamete...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2011.02.032
更新日期:2011-07-01 00:00:00
abstract::Testosterone is required for the maturation of male germ cells, the production of sperm and thus male fertility. However, the mechanisms by which testosterone regulates spermatogenic processes have not been well defined. In this review, classical and non-classical pathways of testosterone signaling in the Sertoli cell...
journal_title:Steroids
pub_type: 杂志文章,评审
doi:10.1016/j.steroids.2008.11.017
更新日期:2009-07-01 00:00:00
abstract::The abilities of a variety of estrogens and antiestrogens to stimulate DNA synthesis in the prepuberal rat uterus were compared. One microgram of each compound was administered in vivo via a single intraperitoneal injection. DNA synthesis was assayed in vitro in isolated nuclei 24 h later. The relative mitogenicities ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(89)90096-2
更新日期:1989-08-01 00:00:00
abstract::We describe a convenient and stereoselective route to the synthesis of 27-hydroxycholesterol. Also its radiolabeled analog, 22, 23 di [(3)H]-27-hydroxycholesterol with high specific radioactivity (55 Ci/mmol) was synthesized by this method. Julia condensation of steroidal 22-sulfone with aldehyde, led to the addition ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(00)00099-4
更新日期:2000-07-01 00:00:00
abstract::It has been shown by others that treatment with only progesterone is sufficient to induce implantation and maintain pregnancy in ovariectomized-adrenalectomized rabbits. Based on these results, it was deduced that induction of implantation in the rabbit does not require estrogen. In making this deduction, "blastocyst ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(76)90017-9
更新日期:1976-10-01 00:00:00
abstract::Explants prepared from the neocortex and the fetal zone of the human fetal adrenal (gestational age 13 to 18weeks) were maintained under conditions of organ culture for 7 to 9 days during which time they were exposed to hACTH and various related peptides. Corticotrophic activity was monitored by the daily release of d...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(78)90037-5
更新日期:1978-04-01 00:00:00
abstract::The inhibition of testosterone 5alpha-reductase activity by 3-oxo-4-androstene-17beta-carboxylic acid in the male reproductive organs of the rat was demonstrated in vitro. The medium for incubation of caput epididymis showed the highest concentration of 5alpha-dihydrotestosterone (5alpha-DHT) whereas the highest conce...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(76)90135-5
更新日期:1976-06-01 00:00:00
abstract::Biotransformation of an orally active contraceptive drug, desogestrel (1), with Cunninghamella elegans yielded a new metabolite, 13β-ethyl-11-methylene-18,19-dinor-17α-pregn-4-en-20-yn-17β-ol-3,6-dione (2), along with five known metabolites, i.e., 13β-ethyl-11-methylene-18,19-dinor-17α-pregn-4-en-20-yn-3β,6β,17β-triol...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2020.108694
更新日期:2020-10-01 00:00:00
abstract::Plant lignans are currently being widely studied for their potential benefits for human health as their consumption has been correlated with lower risks for developing chronic diseases, such as breast cancer and coronary heart disease. However, studies of some classes of lignans, in particular the furofurans, are hamp...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2005.10.005
更新日期:2006-03-01 00:00:00
abstract::Inflammatory cytokines interleukin-1 (IL-1) and tumor necrosis factor-α (TNF-α) regulate the activity of the hypothalamo-pituitary-adrenal (HPA) axis at several levels. Although hypothalamic CRH secretion may be the primary mechanism by which these cytokines activate the HPA axis, IL-1 expression is increased within t...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2011.04.018
更新日期:2011-09-01 00:00:00
abstract::Determinations of oxygen solubility in serum were made utilizing various concentrations of corticosteroids in the serum. Within a certain concentration range dexamethasone and betamethasone caused an increase in oxygen solubility. None of the other steroids tested demonstrated this property. ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(76)90041-6
更新日期:1976-09-01 00:00:00
abstract::The 1,3-dipolar cycloaddition of azomethine ylide derived in situ from the reaction of acenaphthylene-1,2-dione and 1,3-thiazolane-4-carboxylic acid to various exocyclic dipolarophiles synthesized from trans-androsterone and trans-dehydroandrosterone afforded a library of novel spiro[5'.2″]acenaphthylene-1″-one-spiro[...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2012.12.017
更新日期:2013-04-01 00:00:00
abstract::Finasteride is a specific 5 alpha-reductase inhibitor that has been shown to reduce the size of human benign prostatic hyperplasia (BPH) by inhibiting the intraprostatic conversion of testosterone to 5 alpha-dihydrotestosterone. The aim of the present in vitro study was to describe in more detail the inhibitory effect...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(94)90016-7
更新日期:1994-11-01 00:00:00