Chromatographic patterns of urinary ethynyl estrogen metabolites in various populations.

Abstract:

:Radioactive mestranol (ME) and/or ethynylestradiol (EE) were administered to women in Nigeria, Sri Lanka, and the USA, and the types and patterns of radioactive urinary conjugates examined by Sephadex LH-20 chromatography. There are no differences in the total excretion of urinary radioactivity over 3 days. Consistent geographic differences appear to be present in the proportion of 3-, 17-, and 3,17-glucuronides. If confirmed on larger population samples, these observations may indicate significant geographic differences in the hepatic metabolism of ethynyl estrogens. High performance liquid chromatographic patterns of the urinary aglycone metabolites of ME and EE were examined in a number of women. The separation was accomplished on a Chromegaprep Diol column with a gradient of isopropanol in heptane. Ethynyl estrogen metabolism shows considerable individual variation. EE is usually the principal compound escreted following ME or EE administration. Unmetabolized ME is present in the ME profiles. The profiles of EE and ME are similar, with EE demonstrating a more complex pattern. Oxidative metabolism occurs chiefly at positions 2, 6 and 16 and is fairly extensive in the USA subjects. The Sri Lankan women generally show less of the oxidative products and the Nigerian group display a notable lack of oxidative metabolism. There is no difference in the metabolic patterns of long-term oral contraceptive users vs. non-users. Using silver sulfoethylcellulose column chromatography, from 14.1 to 34.7% of the excreted radiolabeled aglycones are non-ethynyl (i.e., either D-homo or de-ethynylated estrogens). :Types and patterns of radioactive urinary conjugates were examined by Sephadex LH-20 chromatography after ingestion of radiolabeled mestranol and/or ethinyl estradiol in a variety of populations. Women in Nigeria, Sri Lanka, and the United States were given the radioactive estrogens. Over 3 days, no differences in total excretion of urinary radioactivity were found. However, there were consistent geographical differences in the proportion of 3-, 17-, and 3,17-glucuronides, indicating significant geographical differences in hepatic metabolism of ethinyl estrogens. Then high-performance liquid chromatographic patterns of urinary aglycone metabolites of mestranol and ethinyl estradiol were studied after successful separation on a Chromegaprep Diol column. Ethinyl estrogen metabolism showed great individual variation. Ethinyl estradiol was the principal compound excreted after ingesting either ethinyl estradiol or mestranol. Unmetabolized mestranol was found as well. Ethinyl estradiol and mestranol profiles were similar, with ethinyl estradiol demonstrating a more complex pattern. U. S. subjects displayed extensive oxidative metabolism (Positions 2, 6, and 16), Sri Lankans less Nigerians very little if any. No difference in metabolic patterns was seen among long-term vs. short-term users vs. nonusers.

journal_name

Steroids

journal_title

Steroids

authors

Williams MC,Goldzieher JW

doi

10.1016/0039-128x(80)90001-x

subject

Has Abstract

pub_date

1980-09-01 00:00:00

pages

255-82

issue

3

eissn

0039-128X

issn

1878-5867

pii

0039-128X(80)90001-X

journal_volume

36

pub_type

杂志文章

相关文献

STEROIDS文献大全
  • Differential binding of dexamethasone to ammonium sulfate precipitates of human adipose tissue cytosols.

    abstract::Saturation analysis of the binding of [3H]dexamethasone [( 3H]DEX) to ammonium sulfate precipitates (ASPs) confirmed the presence of a limited-capacity, high-affinity binder in human adipose tissue cytosols. Various non-radioactive steroids competed with [3H]DEX for binding to the ASPs in the following sequence: dexam...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/0039-128x(87)90091-2

    authors: Miller LK,Kral JG,Strain GW,Zumoff B

    更新日期:1987-06-01 00:00:00

  • Preparation of the N-(4'-hydroxy-[3'-125I]iodophenethyl)-6-(4-O-diethylstilbestryl)hexanam ide for diethylstilbestrol radioimmunoassays.

    abstract::The synthesis of a radioiodinated diethylstilbestrol (DES) derivative is described. This derivative was prepared by coupling the previously synthesized active ester of 6-(4-O-diethylstilbestryl)hexanoic acid with mono-[125I]iodotyramine in dry tetrahydrofuran (20 to 22 C, 16 hours). The mono-[125I]iodotyramine was pre...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/0039-128x(92)90092-n

    authors: Economou EV,Livaniou E,Evangelatos GP,Ithakissios DS

    更新日期:1992-01-01 00:00:00

  • Carbenoxolone does not cause a syndrome of mineralocorticoid excess in sheep.

    abstract::These studies investigated whether treatment with carbenoxolone (CBX), an inhibitor of 11 beta-hydroxysteroid dehydrogenase (11 beta-HSD), resulted in an enhanced mineralocorticoid response to endogenous or infused cortisol. In conscious sodium replete sheep with a parotid fistula, infusion of CBX (40 mg/h for 10 days...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/s0039-128x(97)00141-4

    authors: Dodic M,May CN,Coghlan JP

    更新日期:1998-02-01 00:00:00

  • Metabolism of (24R and 24S)-27-nor-24-methyl-3alpha,7alpha-dihydroxy-5beta-cholestan-26-oic acids and 27-nor-3alpha,7alpha-dihydroxy-5beta-cholestan-26-oic acid in guinea pigs.

    abstract::[7beta-3H]-(24R and 24S)-27-nor-24-methyl-3alpha,7alpha-dihydroxy-5beta-cholestan-26-oic acids and [7beta-3H]-27-nor-3alpha,7alpha-dihydroxy-5beta-cholestan-26-oic acid (C27 and C26 bile acids having the same nuclear configuration as cheno-deoxycholic acid and its precursor, 3alpha,7alpha-dihydroxy-5beta-cholestan-26-...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/0039-128x(78)90076-4

    authors: Kihira K,Mosbach EH

    更新日期:1978-12-01 00:00:00

  • Sex differences in sleep apnea and comorbid neurodegenerative diseases.

    abstract::Sleep apnea is a disorder, which increasingly affects people worldwide. Whether the associated hypoxic events during sleep are central or obstructive in origin, the end result is excessive daytime sleepiness and an increased risk for several comorbidities, such as cardiovascular and neurodegenerative disorders. Sleep ...

    journal_title:Steroids

    pub_type: 杂志文章,评审

    doi:10.1016/j.steroids.2017.12.006

    authors: Snyder B,Cunningham RL

    更新日期:2018-05-01 00:00:00

  • Estrogen modulation of K(+) channel activity in hypothalamic neurons involved in the control of the reproductive axis.

    abstract::Here we report on the progress we have made in elucidating the mechanisms through which estrogen alters synaptic responses in hypothalamic neurons. We examined the modulation by estrogen of the coupling of various receptor systems to inwardly rectifying and small conductance, Ca(2+)-activated K(+) (SK) channels. We us...

    journal_title:Steroids

    pub_type: 杂志文章,评审

    doi:10.1016/s0039-128x(01)00181-7

    authors: Kelly MJ,Rønnekleiv OK,Ibrahim N,Lagrange AH,Wagner EJ

    更新日期:2002-05-01 00:00:00

  • Unveiling the molecular mechanism of brassinosteroids: Insights from structure-based molecular modeling studies.

    abstract::Brassinosteroid (BR) phytohormones play indispensable roles in plant growth and development. Brassinolide (BL) and 24-epibrassinolide (24-epiBL) are the most active ones among the BRs reported thus far. Unfortunately, the extremely low natural content and intricate synthesis process limit their popularization in agric...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/j.steroids.2015.09.002

    authors: Lei B,Liu J,Yao X

    更新日期:2015-12-01 00:00:00

  • Adiponectin is involved in the protective effect of DHEA against metabolic risk in aged rats.

    abstract::The aim of the present work was to analyze the effect of dehydroepiandrosterone (DHEA) on several metabolic risk factors, including cardiovascular health and insulin resistance, in aged rats submitted to a high-fat diet. For that, weaned rats were fed on a high-fat diet until 20 months of age. In the last 13 weeks of ...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/j.steroids.2008.04.011

    authors: Pérez-de-Heredia F,Sánchez J,Priego T,Nicolás F,Portillo Mdel P,Palou A,Zamora S,Garaulet M

    更新日期:2008-10-01 00:00:00

  • Estrogen and growth factor receptor interactions in human breast and non-small cell lung cancer cells.

    abstract::Extranuclear estrogen receptors may mediate rapid effects of estradiol that communicate with nuclear receptors and contribute to proliferation of human cancers bearing these signaling proteins. To assess these growth-promoting pathways, we undertook controlled homogenization and fractionation of NIH-H23 non-small cell...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/j.steroids.2005.02.017

    authors: Pietras RJ,Márquez DC,Chen HW,Tsai E,Weinberg O,Fishbein M

    更新日期:2005-05-01 00:00:00

  • Nucleosteroids: carbocyclic nucleoside analogs of androst-4-en-17 beta-ol.

    abstract::Steroidal nucleoside analogs were synthesized starting from testosterone. By reduction of the oxime of 17 beta-hydroxy-androst-4-en-3-one (testosterone), a mixture of the two amino epimers of C-3 were obtained. The 3 alpha-amino-androst-4-en-17 beta-ol was crystallized in 73% yield and coupled with 5-amino-4,6-dichlor...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/s0039-128x(01)00157-x

    authors: Gelpi ME,Cadenas RA,Mosettig J,Zuazo BN

    更新日期:2002-03-01 00:00:00

  • Characterization of desoxymethyltestosterone main urinary metabolite produced from cultures of human fresh hepatocytes.

    abstract::Desoxymethyltestosterone (DMT; 17β-hydroxy-17α-methyl-5α-androst-2-ene) is a designer steroid present in hormonal supplements distributed illegally as such or in combination with other steroids, for self-administration. It figures on the list of substances prohibited in sports and its detection in athlete's urine samp...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/j.steroids.2012.02.008

    authors: Gauthier J,Poirier D,Ayotte C

    更新日期:2012-05-01 00:00:00

  • The 11 beta-hydroxysteroid dehydrogenase type II enzyme: biochemical consequences of the congenital R337C mutation.

    abstract::The 11 beta-hydroxysteroid dehydrogenase type II enzyme (11 beta HSD2) converts cortisol to cortisone, allowing the non-selective mineralocorticoid receptor to bind aldosterone. When the activity of this enzyme is compromised, as occurs in licorice intoxication or in the congenital syndrome of apparent mineralocortico...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/0039-128x(96)00013-x

    authors: Ferrari P,Obeyesekere VR,Li K,Andrews RK,Krozowski ZS

    更新日期:1996-04-01 00:00:00

  • Metabolism of estrogens by rabbit blastocysts: formation of estrogen glucosides and preferential conversion of estrone to estradiol-17 beta.

    abstract::When day 6 rabbit blastocysts were cultured (3 embryos/mL) in medium 199 containing 3.68 microM estradiol-17 beta (E2), 40% of E2 was metabolized in 24 h, at a rate of 18 pmol/embryo(b)/h, yielding 4 major metabolite fractions. Two of them were identified to be estrogen glucosides: 17 beta-hydroxyestra-1,3,5(10)-trien...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/0039-128x(89)90053-6

    authors: Wu JT,Williams KI

    更新日期:1989-10-01 00:00:00

  • A necessary role for reduced intracellular potassium during the DNA degradation phase of apoptosis.

    abstract::Apoptosis is a highly organized mechanism that allows specific cells to die in a controlled manner. Apoptosis can be induced by a variety of agents in a great number of cell types, but cell shrinkage and discrete chromatin cleavage remain common components of apoptosis that is manifested by constitutive enzymes. Despi...

    journal_title:Steroids

    pub_type: 杂志文章,评审

    doi:10.1016/s0039-128x(99)00034-3

    authors: Montague JW,Bortner CD,Hughes FM Jr,Cidlowski JA

    更新日期:1999-09-01 00:00:00

  • Reversible and the irreversible structural alterations on brain after resolution of hypercortisolism in Cushing's disease.

    abstract::The adverse effects of hypercortisolism on the human brain have been highlighted in previous studies of Cushing's disease (CD). However, the reversibility of brain damage after the resolution of hypercortisolism remains unclear. Thus, we studied the potential volumetric reversibility in biochemically remitted CD patie...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/j.steroids.2019.108457

    authors: Jiang H,Liu C,Pan SJ,Ren J,He NY,Sun YH,Bian LG,Yan FH,Yang WJ,Sun QF

    更新日期:2019-11-01 00:00:00

  • Hyfraxins A and B, cytotoxic ergostane-type steroid and lanostane triterpenoid glycosides from the invasive ash dieback ascomycete Hymenoscyphus fraxineus.

    abstract::A virulent culture of Hymenoscyphus fraxineus, the causal agent of ash dieback, was investigated for its production of secondary metabolites in a 70 L batch fermentation. Chemical analysis of the mycelial extract by means of flash chromatography and preparative HPLC led to the isolation of a new ergostane-type steroid...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/j.steroids.2018.03.007

    authors: Surup F,Halecker S,Nimtz M,Rodrigo S,Schulz B,Steinert M,Stadler M

    更新日期:2018-07-01 00:00:00

  • Withanolides from the stems and leaves of Physalis pubescens and their cytotoxic activity.

    abstract::A phytochemical study of Physalis pubescens L. afforded twelve compounds, including six new withanolides (1, 4, and 6i-9), four new withanolide glucosides (2, 3, 5, and 6), and two known withanolides (10 and 11). Their structures were established via extensive spectroscopic analysis. The absolute configuration of 3 wa...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/j.steroids.2016.09.002

    authors: Xia G,Li Y,Sun J,Wang L,Tang X,Lin B,Kang N,Huang J,Chen L,Qiu F

    更新日期:2016-11-01 00:00:00

  • A radioimmunoassay for 7 alpha-hydroxy dehydroepiandrosterone in human plasma.

    abstract::A radioimmunoassay for plasma 3 beta, 7 alpha-dihydroxy-5-androsten-17-one (7 alpha-hydroxy DHA) has been developed using anti-sera raised against 3 beta, 7 alpha-dihydroxy-5-androstene-17 beta-carboxyl-bovine serum albumin conjugate and [1, 2 (n) - 3H] 7 alpha-hydroxy DHA as the radioligand. Significant cross reactiv...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/0039-128x(77)90023-x

    authors: Skinner SJ,Tobler CJ,Couch RA

    更新日期:1977-09-01 00:00:00

  • Ratios of plasma and salivary testosterone throughout puberty: production versus bioavailability.

    abstract::Because diffusion of testosterone (T) into the salivary gland is thought to be largely limited to the free, biologically active fraction, salivary testosterone is expected to provide a better measure of testosterone bioavailability in the body than is plasma testosterone. Matched saliva and blood spot samples were col...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/0039-128x(96)00043-8

    authors: Rilling JK,Worthman CM,Campbell BC,Stallings JF,Mbizva M

    更新日期:1996-06-01 00:00:00

  • Circadian and seasonal variations of glucocorticoid receptors in normal human lymphocytes.

    abstract::Circulating human lymphocytes are known to contain specific glucocorticoid receptors. Using a competitive binding whole cell assay, we have examined the binding of [3H] dexamethasone to peripheral lymphocytes of normal male subjects. Lymphocytes were found to contain 2000-10000 glucocorticoid receptor sites/cell and t...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/0039-128x(86)90098-x

    authors: Schlaghecke R,Kley HK

    更新日期:1986-04-01 00:00:00

  • Steroids and genes related to steroid biosynthesis in the female giant freshwater prawn, Macrobrachium rosenbergii.

    abstract::The giant freshwater prawn, Macrobrachium rosenbergii, is important to many Asian countries due to its high economic value as an aquaculture product. With demand increasing, there is requirement for a better understanding of the biosynthetic components that regulate its growth and reproduction, including steroids, in ...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/j.steroids.2016.01.006

    authors: Thongbuakaew T,Siangcham T,Suwansa-ard S,Elizur A,Cummins SF,Sobhon P,Sretarugsa P

    更新日期:2016-03-01 00:00:00

  • Steroidogenic activity of hACTH and related peptides on the human neocortex and fetal adrenal cortex in organ culture.

    abstract::Explants prepared from the neocortex and the fetal zone of the human fetal adrenal (gestational age 13 to 18weeks) were maintained under conditions of organ culture for 7 to 9 days during which time they were exposed to hACTH and various related peptides. Corticotrophic activity was monitored by the daily release of d...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/0039-128x(78)90037-5

    authors: Branchaud CT,Goodyer CG,Hall CS,Arato JS,Silman RE,Giroud CJ

    更新日期:1978-04-01 00:00:00

  • Adrenalectomy amplifies aldosterone induced injury in cardiovascular tissue: an effect attenuated by adrenally derived steroids.

    abstract::Aldosterone induces fibrotic changes in cardiovascular tissues but its effects have usually been demonstrated in models of pre-existing renal injury and/or hypertension. This study tests the hypothesis that aldosterone can directly induce vascular fibrotic changes in the absence of prior renal injury or hypertension. ...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/j.steroids.2012.12.007

    authors: Brem AS,Morris DJ,Li X,Ge Y,Shaw S,Gong R

    更新日期:2013-03-01 00:00:00

  • Reduced urinary aldosterone excretion rates with normal plasma concentrations of aldosterone in the very elderly.

    abstract::Although aldosterone production declines with age, so does the aldosterone metabolic clearance rate (MCR), and the net effect of age on the circulating level of aldosterone may be less than can be predicted from production rates alone. The effect of age on aldosterone production and plasma levels was studied in a grou...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/0039-128x(88)90191-2

    authors: Pratt JH,Hawthorne JJ,Debono DJ

    更新日期:1988-01-01 00:00:00

  • Synthesis and spectroscopic analysis of modified bile salts.

    abstract::For the study of hepatic bile acid transport in vivo, a series of modified bile salts were synthesized. The N-cholyl derivatives of L-leucine, L-alanine, D-alanine, beta-alanine, L-proline, and gamma-amino-butyric acid were prepared from cholic acid, ethyl chloroformate and the corresponding amino acid. Structural ana...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/0039-128x(83)90007-7

    authors: Ballatore AM,Beckner CF,Caprioli RM,Hoffman NE,Liehr JG

    更新日期:1983-02-01 00:00:00

  • Between basic and applied research: Ciba's involvement in steroids in the 1950s and 1960s.

    abstract::Through its collaboration with the ETH in Zurich (Ruzicka's group) and later on with the University of Basel (Reichstein's group). Ciba became involved very early in steroid chemistry. The main task of the chemists consisted at that time of the synthesis of natural hormones and their derivatives. Cholesterol served fo...

    journal_title:Steroids

    pub_type: 历史文章,杂志文章

    doi:10.1016/0039-128x(96)00040-2

    authors: Heusler K,Kalvoda J

    更新日期:1996-08-01 00:00:00

  • Development of a new sensitive and specific time-resolved fluoroimmunoassay (TR-FIA) of chlormadinone acetate in the serum of treated menopausal women.

    abstract::We describe the development of a serum chlormadinone acetate (CMA) time-resolved fluoroimmunoassay (TR-FIA). We prepared haptens (3-CMO-chlormadinone acetate and 6-chloropregna-4,6-dien-17,20-diol-3-one-20-hemisuccinate), biotinylated tracers (3(biotinylaminopropylamido) 3-CMO-chlormadinone acetate and 3-(6-chloropreg...

    journal_title:Steroids

    pub_type: 临床试验,杂志文章

    doi:10.1016/s0039-128x(02)00050-8

    authors: Fiet J,Giton F,Auzerie J,Galons H

    更新日期:2002-12-01 00:00:00

  • All progestins are not created equal.

    abstract::A variety of progestins are available for therapeutic use. It is convenient to classify them into those related in chemical structure to progesterone or testosterone. Progestins related to progesterone can be subdivided into pregnanes and 19-norpregnanes, whereas those related to testosterone can be subdivided into th...

    journal_title:Steroids

    pub_type: 杂志文章,评审

    doi:10.1016/j.steroids.2003.08.003

    authors: Stanczyk FZ

    更新日期:2003-11-01 00:00:00

  • Comparison of flavonoids and isoflavonoids to inhibit rat and human 11β-hydroxysteroid dehydrogenase 1 and 2.

    abstract::Many flavonoids and isoflavonoids have anti-diabetic effects in animal models. However, the mechanisms that are involved are generally unclear. Since 11β-hydroxysteroid dehydrogenases (HSD11Bs) play important roles in diabetes, we hypothesize that flavonoids and isoflavonoids may affect diabetes by targeting two isofo...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/j.steroids.2018.01.013

    authors: Zhu Q,Ge F,Dong Y,Sun W,Wang Z,Shan Y,Chen R,Sun J,Ge RS

    更新日期:2018-04-01 00:00:00

  • Quantification of endogenous testosterone and dihydrotestosterone in fetal tissues from male guinea-pig.

    abstract::Testosterone (17 beta -hydroxy-4-androsten-3-one ; T) and dihydrotestosterone (17 beta -hydroxy-5 alpha -androstan-3-one ; DHT) concentrations were determined by radioimmunoassay in different fetal tissues taken from male guinea-pigs. Androgen concentrations were maximal in the components of the Wolffian duct (vas def...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/0039-128x(81)90032-5

    authors: Rigaudière N

    更新日期:1981-08-01 00:00:00