Abstract：
:An enzyme immunoassay for estrone in swine serum was established. For this, beta-galactosidase from E. coli was conjugated through estrone-17 (O-carboxymethyl)oxime using a mixed anhydride reaction. The percentage of immunoreactive estrone-17 (O-carboxymethyl)oxime-beta-galactosidase conjugate was estimated to be about 70%. The recovery rate of estrone (25-500 pg) added to 0.05 ml of swine serum averaged 91.4%. The sensitivity of the present enzyme immunoassay was 5 pg/tube. The coefficients of variation (CV) were 5.9-8.2% (within assays) and 4.1-5.9% (between assays), respectively. Estrone values determined by the present enzyme immunoassay were highly correlated with those determined by radioimmunoassay (r = 0.99, P less than 0.005). This method of enzyme immunoassay was determined to be suitable for the routine assay of serum estrone.
journal_name
Steroidsjournal_title
Steroidsauthors
Tamamura F,Nakao T,Tsunoda N,Kawata Kdoi
10.1016/0039-128x(82)90136-2subject
Has Abstractpub_date
1982-06-01 00:00:00pages
657-66issue
6eissn
0039-128Xissn
1878-5867pii
0039-128X(82)90136-2journal_volume
39pub_type
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