Abstract:
:A simplified, non-chromatographic method for aldosterone-gamma-lactone (2) radio-immunoassay is described, using a high titer aldosterone-gamma-lactone antibody. This method takes six working hours for completion; the blanks are below the sensitivity of the assay (0-10 pg), and the intra and interassay coefficient of variation is 4.9% and 8.2% respectively. There is no significant variation with different plasma volumes assayed and the plot of added and assayed aldosterone gives a slope of 0.983. The negligible cross reactivity with other steroids and lactones eliminates the problem of interference by these substances.
journal_name
Steroidsjournal_title
Steroidsauthors
Antunes JR,Dale SL,Melby JCdoi
10.1016/0039-128x(76)90004-0subject
Has Abstractpub_date
1976-11-01 00:00:00pages
621-30issue
5eissn
0039-128Xissn
1878-5867pii
0039-128X(76)90004-0journal_volume
28pub_type
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