Abstract:
:Nucleocytoplasmic shuttling of glucocorticoid receptors (GRs) is disrupted in v-mos-transformed cells leading to the redistribution of hormone-bound receptors from the nuclear to cytoplasmic compartments. We show here that GRs from v-mos-transformed cells are hyperphosphorylated on a specific peptide and maintain hormone-induced phosphorylations upon a prolonged hormone treatment that is associated with disruptions in its nucleocytoplasmic shuttling. Since similar effects on GR nucleocytoplasmic shuttling and phosphorylation were exerted upon treatment of nontransformed cells with the protein phosphatase inhibitor okadaic acid, we examined whether hyperphosphorylation of GRs in v-mos-transformed cells resulted from inhibition of receptor dephosphorylation. Protein phosphatase activity, measured using various substrates in vitro, was identical in cell-free extracts prepared from v-mos-transformed and nontransformed cells. Analysis of phosphate turnover in vivo from either the sum of all GR phosphorylation sites or from individual sites using pulse-chase analysis, did not reveal any significant difference between v-mos-transformed cells versus nontransformed cells. Thus, hyperphosphorylation of GR in v-mos-transformed cells does not appear to result from inhibition of GR dephosphorylation, but rather from stimulation of GR phosphorylation.
journal_name
Steroidsjournal_title
Steroidsauthors
Borror KC,Garabedian MJ,DeFranco DBdoi
10.1016/0039-128x(94)00068-nsubject
Has Abstractpub_date
1995-05-01 00:00:00pages
375-82issue
5eissn
0039-128Xissn
1878-5867pii
0039-128X(94)00068-Njournal_volume
60pub_type
杂志文章相关文献
STEROIDS文献大全abstract::In an attempt to synthesize compounds with selective estrogen-receptor binding, fluoro- and amino-clomiphene were totally synthesized from benzyl chloride, and their estrogenic/antiestrogenic activity as well as that of some of their chemical intermediates was evaluated. The triazene prepared from the amino-clomiphene...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(86)90042-5
更新日期:1986-07-01 00:00:00
abstract::A virulent culture of Hymenoscyphus fraxineus, the causal agent of ash dieback, was investigated for its production of secondary metabolites in a 70 L batch fermentation. Chemical analysis of the mycelial extract by means of flash chromatography and preparative HPLC led to the isolation of a new ergostane-type steroid...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2018.03.007
更新日期:2018-07-01 00:00:00
abstract::A series of steroidal and nonsteroidal Michael acceptors that represent reaction products for 3 alpha-hydroxysteroid dehydrogenase were synthesized and evaluated as potential enzyme-generated inactivators. Introduction of exocyclic olefins either at C-2 or C-6 produced inhibitors with high affinity for the enzyme (0.0...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(91)90030-y
更新日期:1991-08-01 00:00:00
abstract::The left adrenal of two female dogs were perfused with either 3 muCi [4-14C]cpd. S (2) and 45 muCi [1, 2-3H] 21-deoxycortisone (Dog I), or with 1 muCi [4-14C] 17-hydroxyprogesterone and 65 muCi [1,2-3H] 21-deoxycortisone (Dog II). In Dog I, 40% of the perfused 21-deoxycortisone was converted to cortisone and 23% of cp...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(77)90078-2
更新日期:1977-08-01 00:00:00
abstract::The first natural occurrence of 19-norcholestenone is reported, together with 17 sterols and one other delta 4-3-ketone in the extracts of the Californian gorgonian, Muricea californica (Aurivillius). Six additional demethyl sterols and five additional 4-monomethyl sterols which remain unidentified were also detected....
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(83)90093-4
更新日期:1983-04-01 00:00:00
abstract::Tibolone is a highly effective postmenopausal hormone treatment that has its biological activity dependent on metabolism to 3alpha- and 3beta-OH tibolone, which bind solely to the estrogen receptor. Despite the high levels of estrogen receptor-binding metabolites in the circulation, the endometrium becomes atrophic, s...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2005.04.010
更新日期:2005-11-01 00:00:00
abstract::The accurate analysis of trace components in complex biological matrices requires the use of reliable internal standards. For liquid chromatography/mass spectrometry analysis, the stable isotope-labeled analogues of the analyte molecules are the most appropriate internal standards. In this paper the synthesis of the 3...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2012.08.007
更新日期:2012-11-01 00:00:00
abstract::The conjugation reactions of hydration and dehydrogenation catalyzed by the dehydratase and dehydrogenase activities of D-3-hydroxyacyl-CoA dehydratase/D-3-hydroxyacyl-CoA dehydrogenase bifunctional protein (DBP) and enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase bifunctional protein (LBP) in the side chain degra...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(00)00217-8
更新日期:2001-02-01 00:00:00
abstract::Breast cancer risk is strongly related to several reproductive and hormonal factors, but the nature of the effects of endogenous oestrogens has been difficult to establish. Data are now available from several large prospective studies with biobanks of stored serum, enabling better characterization of the associations ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2011.02.029
更新日期:2011-07-01 00:00:00
abstract::When day 6 rabbit blastocysts were cultured (3 embryos/mL) in medium 199 containing 3.68 microM estradiol-17 beta (E2), 40% of E2 was metabolized in 24 h, at a rate of 18 pmol/embryo(b)/h, yielding 4 major metabolite fractions. Two of them were identified to be estrogen glucosides: 17 beta-hydroxyestra-1,3,5(10)-trien...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(89)90053-6
更新日期:1989-10-01 00:00:00
abstract::The palladium-catalyzed coupling of various 17-iodo-delta 16 steroids (17-iodo-androst-16-ene, 17-iodo-4-methyl-4-aza-androst-16-en-3-one, and 17-iodo-4-aza-androst-16-en-3-one) with dialkyl phosphites (dimethyl phosphite, diethyl phosphite, and diisopropyl phosphite) was examined in detail. The only successful condit...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(95)00121-6
更新日期:1995-12-01 00:00:00
abstract::Postmitochondrial supernatant (PMS) (1) has been prepared from the homogenate of rat seminal vesicles and the characteristics of the binding reaction of 5alpha-dihydrotestosterone (DHT) to the cytoplasmic androgen receptor have been studied using a charcoal adsorption procedure. At 0 degrees C apparent equilibrium of ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(77)80021-4
更新日期:1977-12-01 00:00:00
abstract::Structural transformation of the steroidal lactone, oxandrolone (1), by suspended-cell cultures of the plant pathogen fungus Rhizopus stolonifer, resulted in the production of three new metabolites. These metabolites were identified as 11alpha-hydroxyoxandrolone (2), 6alpha-hydroxyoxandrolone (3) and 9alpha-hydroxyoxa...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2009.08.003
更新日期:2009-11-01 00:00:00
abstract::Bile acid derivatives, with and without C-3 sulfate groups, and having either the diazo- or halomethylketone moieties, have been synthesized in good yield and purity. The synthetic sequence, COOH leads to COC1 leads to COCHN2 leads to COCH2X, was used with deoxycholic and cholic acids, which requires carefully control...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(79)90046-1
更新日期:1979-08-01 00:00:00
abstract::Androgens including testosterone and dihydrotestosterone play important roles on brain structure and function, either directly through androgen receptor or indirectly through estrogen receptors, which need coactivators for their transcription activation. Steroid receptor coactivator-1 (SRC-1) has been shown to be mult...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2014.06.006
更新日期:2014-10-01 00:00:00
abstract::Explants prepared from the neocortex and the fetal zone of the human fetal adrenal (gestational age 13 to 18weeks) were maintained under conditions of organ culture for 7 to 9 days during which time they were exposed to hACTH and various related peptides. Corticotrophic activity was monitored by the daily release of d...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(78)90037-5
更新日期:1978-04-01 00:00:00
abstract::Ventral prostate was used as a system to study the nature and properties of microsomal androgen receptor. The endoplasmic reticulum from rat ventral prostate contains high-affinity, low-capacity binding sites for androgen that are intrinsic to this intracellular compartment. Microsomal androgen receptors are not due t...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(91)90126-g
更新日期:1991-02-01 00:00:00
abstract::A variety of compounds, including the selective estrogen receptor (ER) modulators tamoxifen and raloxifene, phytoestrogens such as genistein, and xenoestrogens such as bisphenol, bind to the estrogen receptor and elicit biological responses. Structural studies have linked the altered activity of compounds such as 4-hy...
journal_title:Steroids
pub_type: 杂志文章,评审
doi:10.1016/j.steroids.2004.03.014
更新日期:2004-06-01 00:00:00
abstract::To determine the sensitivity of the overnight 1-mg dexamethasone suppression test in diagnosing Cushing's syndrome, we evaluated the cortisol responses of 55 subjects (25 non-obese individuals with body mass index less than 25 kg/m2, 20 obese individuals with body mass index greater than 30 kg/m2, and 10 patients with...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(91)90011-j
更新日期:1991-11-01 00:00:00
abstract::The biosynthesis of progesterone from [3H]pregnenolone was curvilinear over a 6 h time course in human placenta cytotrophoblasts and in human placenta choriocarcinoma cells (JEG-3 cells). Mass measurements determined independently by radioimmunoassay indicate that the progesterone synthesized by cytotrophoblasts (21.0...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(88)90043-8
更新日期:1988-05-01 00:00:00
abstract::This study was designed to describe the time-course changes of catabolic proteins following muscle atrophy induced by 10 days of dexamethasone (DEX). Rats underwent DEX treatment for 1, 3, 5, 7 and 10 days. Body weight (BW) and lean mass were obtained using a dual energy X-ray absorptiometry (DEXA) scan. Muscle ringer...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2015.12.016
更新日期:2016-03-01 00:00:00
abstract::The addition of molybdate to rabbit liver cytosol increased significantly the affinity of the glucocorticoid receptor for [3H] dexamethasone without influencing the concentration of binding sites. This effect was concentration dependent. Analysis of the binding data by curve-fitting and Scatchard plot revealed the occ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(84)90088-6
更新日期:1984-08-01 00:00:00
abstract::Dehydroepiandrosterone (DHEA) supplementation has been anecdotally considered as a tool to improve body composition and health status. We aimed to verify the impact of DHEA supplementation on traditional measurements of body composition and blood pressure (BP) due to their clinical applicability. A meta-analysis of ra...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2020.108710
更新日期:2020-11-01 00:00:00
abstract::Besides regulating water and electrolyte homeostasis, the mineralocorticoid receptor (MR) elicits pathophysiological effects in the renocardiovascular system. Although the MR's closest relative, the glucocorticoid receptor (GR), acts as a transcription factor at the same hormone-response-element (HRE), activated gluco...
journal_title:Steroids
pub_type: 杂志文章,评审
doi:10.1016/j.steroids.2009.10.006
更新日期:2010-08-01 00:00:00
abstract:BACKGROUND:Urinary steroid profiling by GC or GC-MS are established clinical tools to complement other biochemical tests in the diagnosis and investigation of a wide range of adrenocortical disorders, but normative data on adults using the more specific GC-MS are lacking. Our objective was to set up the reference inter...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2008.03.004
更新日期:2008-09-01 00:00:00
abstract::The steroid nucleus is an interesting scaffold for the development of new therapeutic agents. Within the goal of identifying anticancer agents, new pregnane derivatives were prepared by using a sequence of liquid and solid-phase reactions. After we dehydrated epi-allopregnanolone in one step with diethylaminosulfur tr...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2012.07.012
更新日期:2012-11-01 00:00:00
abstract:BACKGROUND:Congenital adrenal hyperplasia (CAH) caused by 21-hydroxylase deficiency (21OHD) is an autosomal recessive disorder due to mutation in the CYP21A2 gene. OBJECTIVE:To elucidate the genetic basis of 21-hydroxylase-deficient CAH in Hong Kong Chinese patients. PATIENTS AND METHODS:Mutational analysis of the CY...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2011.04.010
更新日期:2011-09-01 00:00:00
abstract::Fingernail clipping is expected to be a specimen for steroid testing, because it has several advantages over blood; i.e., noninvasive collection, ease of storage, portability and handling, and possibility for an assessment of the steroid status over a relatively long and retrospective time window. In this study, we ex...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2016.02.013
更新日期:2016-05-01 00:00:00
abstract::During the periparturient period, dairy cows are subjected to physiological changes that may induce immunosuppression and an increased susceptibility of the animal to bacterial infections such as mastitis. The incidence of clinical environmental mastitis is high during the last period of gestation, at parturition and ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(03)00040-0
更新日期:2003-05-01 00:00:00
abstract::Two new 4-methyl-progesteroids, nodulisporisteriod A (1) and nodulisporisteriod B (2), were isolated from the extract of an endolichenic fungal strain Nodulisporium sp. (No. 65-17-2-1), along with two related metabolites, demethoxyviridin (3) and inoterpene B (4). Their structures were determined by detailed spectrosc...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2013.05.007
更新日期:2013-09-01 00:00:00