The synthesis of diazo, halo, and sulfoxy bile acid derivatives: potential affinity labels.

abstract：:Testicular homogenates of tamoxifen-treated rats were incubated with labeled steroid precursors (progesterone, 17 alpha-hydroxyprogesterone, dehydroepiandrosterone, androstenedione or testosterone) in order to study the effect of tamoxifen on testicular steroidogenesis. The results indicate that a 9 day treatment with...

journal_title：Steroids

authors： Vanderstichele H,Eechaute W,Lacroix E,Leusen I

更新日期：1989-06-01 00:00:00

abstract：:A radioimmunoassay for plasma 3 beta, 7 alpha-dihydroxy-5-androsten-17-one (7 alpha-hydroxy DHA) has been developed using anti-sera raised against 3 beta, 7 alpha-dihydroxy-5-androstene-17 beta-carboxyl-bovine serum albumin conjugate and [1, 2 (n) - 3H] 7 alpha-hydroxy DHA as the radioligand. Significant cross reactiv...

journal_title：Steroids

authors： Skinner SJ,Tobler CJ,Couch RA

更新日期：1977-09-01 00:00:00

abstract：:Steroid hormones are involved in the regulation of a variety of processes like embryonic development, sex differentiation, metabolism, immune responses, circadian rhythms, stress response, and reproduction in vertebrates. Teleost fishes and humans show a remarkable conservation in many developmental and physiological ...

journal_title：Steroids

authors： Tokarz J,Möller G,Hrabě de Angelis M,Adamski J

更新日期：2015-11-01 00:00:00

abstract：:A series of amphiphilic derivatives of (3β,17β)-3-hydroxyandrost-5-ene-17-carboxylic acid (1) with the polyamine spermine and three other diamines, 1,2-diaminoethane, piperazine and cadaverine, were synthesized and their antimicrobial activity and cytotoxicity were investigated. Among the target compounds, several one...

journal_title：Steroids

authors： Özdemir Z,Bildziukevich U,Šaman D,Havlíček L,Rárová L,Navrátilová L,Wimmer Z

更新日期：2017-12-01 00:00:00

abstract：:Plasma concentration measurements of 13C-labeled cortisol ([1,2,4,19-13C(4)]cortisol, cortisol-13C(4)) and its metabolite cortisone-13C(4) were made simultaneously with measurements of endogenous cortisol and cortisone by gas chromatography-mass spectrometry (GC-MS). After administering a small amount (3mg) of cortiso...

journal_title：Steroids

authors： Kasuya Y,Yokokawa A,Shibasaki H,Furuta T

更新日期：2002-08-01 00:00:00

abstract：:A highly sensitive and specific quantification method of estrone and estradiol in human serum was described based upon the use of picolinoyl derivatization and liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) in a positive mode. Estrogens were treated with picolinoyl chloride hydrochloride o...

journal_title：Steroids

authors： Yamashita K,Okuyama M,Watanabe Y,Honma S,Kobayashi S,Numazawa M

更新日期：2007-10-01 00:00:00

abstract：:We have previously demonstrated that 1α,25 dihydroxy-vitamin D(3) (1α,25(OH)(2)D(3)) has antiproliferative effects on the growth of endothelial cells transformed by the viral G protein-coupled receptor associated to Kaposi sarcoma (vGPCR). In this work, we have investigated whether 1α,25(OH)(2)D(3) exerts its growth i...

journal_title：Steroids

authors： Gonzalez-Pardo V,D'Elia N,Verstuyf A,Boland R,Russo de Boland A

更新日期：2012-09-01 00:00:00

abstract：:Optimal assay conditions for cholesterol 7 alpha-hydroxylase activity in swine liver microsomes were determined. The enzyme activity is induced three-fold by feeding cholestyramine to the swine. This suggests that cholesterol 7 alpha-hydroxylase is likely to be the rate-limiting enzyme for biosynthesis of bile acids i...

journal_title：Steroids

authors： Li JR,Kim DN

更新日期：1979-03-01 00:00:00

abstract：:Human progesterone receptor (PR) contains a polyproline motif in the amino-terminal domain that interacts with the SH3 domain of Src and mediates rapid activation of c-Src and downstream MAPK (Erk-1/-2) independent of the transcriptional activity of PR. Forcedly target PR to different locations in the cell by use of m...

journal_title：Steroids

authors： Boonyaratanakornkit V,Bi Y,Rudd M,Edwards DP

更新日期：2008-10-01 00:00:00

abstract：:It has been shown by others that treatment with only progesterone is sufficient to induce implantation and maintain pregnancy in ovariectomized-adrenalectomized rabbits. Based on these results, it was deduced that induction of implantation in the rabbit does not require estrogen. In making this deduction, "blastocyst ...

journal_title：Steroids

authors： Dey SK,Dickmann Z,Sen Gupta J

更新日期：1976-10-01 00:00:00

abstract：:The synthesis of the esters of norethisterone (17 alpha-ethynyl-17 beta-hydroxyestr-4-en-3-one) with three bile acids and of the cholesteryl carbonate of norethisterone are described. :This article reports the synthesis of the esters of norethisterone (17 alpha-ethynl-17beta-hydroxyestr-4-en-3-one) with 3 bile acids ...

journal_title：Steroids

authors： Herz JE,Sandoval J

更新日期：1983-03-01 00:00:00

abstract：:The effect of sodium intake on the aldosterone metabolic clearance rate (MCR) was examined in 5 normal subjects. Measurements were made under conditions where dietary sodium ranged from 10 to 1500 meq/day. There were no consistent changes in MCR over these extremes of sodium intake, and the plasma level of aldosterone...

journal_title：Steroids

authors： Pratt JH,Luft FC,Parkinson CA,Rankin LI

更新日期：1981-01-01 00:00:00

abstract：:18,20-Epoxy-11 beta,17 alpha,20 beta,21-tetrahydroxypregn-4-en-3-one was synthesized by the application of hypoiodite reaction to the cortisol acetonide. The intermediary 18-iodo derivative was converted to the 11-oxo steroid by chromic acid prior to silver ion-assisted solvolysis. Removal of the protective group with...

journal_title：Steroids

authors： Kurosawa T,Ikegawa S,Chiba H,Ito Y,Nakagawa S,Kobayashi K,Tohma M

更新日期：1992-09-01 00:00:00

abstract：:Addition of soluble supernatant to testis microsomes results in 42% increase in steroid 17,20-lyase activity and a 65% increase in 17alpha-hydroxylase activity. This stimulatory activity could be partially purified by salt fractionation. The activating factor(s) was not removed by dialysis nor did it appear to be lipi...

journal_title：Steroids

authors： Betz G,Tsai P

更新日期：1978-10-01 00:00:00

abstract：:Oleoyl-estrone (OE) induces a marked loss of body fat in rats by maintaining energy expenditure, body protein and blood glucose despite decreasing food intake. OE increases glucocorticoids, but they arrest OE lipid-mobilization. We studied here whether OE induces a direct effect on adrenal glands function as part of t...

journal_title：Steroids

authors： Romero Mdel M,Vilà R,Fernández-López JA,Esteve M,Alemany M

更新日期：2010-01-01 00:00:00

abstract：:A cytochrome P450-deficient mutant of the pathogenic fungus, Candida albicans, which accumulates exclusively 14 alpha-methylsterols in place of the normal end product sterol, ergosterol, was examined for alterations in membrane fluidity by electron paramagnetic resonance. The results using four nitroxyl spin labels in...

journal_title：Steroids

authors： Lees ND,Kleinhans FW,Broughton MC,Pennington DE,Ricker VA,Bard M

更新日期：1989-03-01 00:00:00

abstract：:Synthetic vitamin D3 sulfate was prepared by reacting cholecalciferol with sulfamic acid in pyridine. Vitamin D3 sulfate ammonium salt was purified by crystallisation and transformed in sulfate sodium salt. Homogeneity was controlled by reverse phase high pressure liquid chromatography (HPLC). Purified synthetic vitam...

journal_title：Steroids

authors： Boulch NL,Cancela L,Miravet L

更新日期：1982-04-01 00:00:00

abstract：:The formation of a horseradish peroxidase-testosterone conjugate for the enzyme-linked immunoassay of testosterone was investigated, using tritiated testosterone to follow the reaction. The formation of testosterone-3-(carboxymethyl) oxime-peroxidase by the mixed anhydride method was found to give a conjugate of high ...

journal_title：Steroids

authors： Rajkowski KM,Cittanova N,Desfosses B,Jayle MF

更新日期：1977-05-01 00:00:00

abstract：:Inhibition of 5alpha-reduction of testosterone by an anti-androgen TSAA-291 (16beta-ethyl-17beta-hydroxy-4-estren-3-one) was studied in rat ventral prostates and the metabolic conversion of 3H-TSAA-291 was examined both in vitro and in vivo. In the in vitro experiment using nuclear 5alpha-reductase of the prostate, 5a...

journal_title：Steroids

authors： Sudo K,Yoshida K,Akinaga Y,Nakayama R

更新日期：1981-07-01 00:00:00

abstract：:For the purpose of describing the pathway by which estrones are synthesized in the rhesus monkey (Macaca mulatta) corpus luteum (CL), CL were obtained during the midluteal phase of the menstrual cycle and fragments incubated with equimolar amounts of [7-3H]pregnenolone plus [4-14C]progesterone. Metabolites including 3...

journal_title：Steroids

authors： Sholl SA,Wolf RC

更新日期：1980-08-01 00:00:00

abstract：:The mass spectral elimination of water in epimeric 1,3-diols of vitamin D3 (colecalciferol) series has been investigated. It was found that the mass spectra of these steroisomers differ sharply in the relative intensities of the ions M-H2O (m/e 382) and a-H2O (m/e +/- 34), where ion a (formed via formal cleavage of th...

journal_title：Steroids

authors： Zaretskii ZV

更新日期：1979-05-01 00:00:00

abstract：:Some properties of immunoreactive estradiol secreted by adrenals and ovaries of immature female rats were studied. It was shown that adrenals and ovaries of 15-day-old animals secrete approximately equal amounts of immunoreactive estradiol, with thin-layer chromatogram of immunoreactive material being identical for bo...

journal_title：Steroids

authors： Tinnikov AA,Bazhan NM,Yakovleva TV

更新日期：1992-04-01 00:00:00

abstract：:Cortisol 21-amine (21-amino-11beta,17-dihydroxy-4-pregnene-3,20-dione) was prepared and an enzyme immunoassay for cortisol in serum was established using cortisol 21-amine conjugated with alkaline phosphatase. The minimal amount of cortisol detected was 1ng/tube and the measurable range was from 1 to 80 microgram/d1, ...

journal_title：Steroids

authors： Kobayashi Y,Ogihara T,Amitani K,Watanabe F,Kiguchi T,Ninomiya I,Kumahara Y

更新日期：1978-07-01 00:00:00

abstract：:Structural transformation of the steroidal lactone, oxandrolone (1), by suspended-cell cultures of the plant pathogen fungus Rhizopus stolonifer, resulted in the production of three new metabolites. These metabolites were identified as 11alpha-hydroxyoxandrolone (2), 6alpha-hydroxyoxandrolone (3) and 9alpha-hydroxyoxa...

journal_title：Steroids

authors： Choudhary MI,Mohammad MY,Musharraf SG,Parvez M,Al-Aboudi A,Atta-ur-Rahman

更新日期：2009-11-01 00:00:00

abstract：:Besides regulating water and electrolyte homeostasis, the mineralocorticoid receptor (MR) elicits pathophysiological effects in the renocardiovascular system. Although the MR's closest relative, the glucocorticoid receptor (GR), acts as a transcription factor at the same hormone-response-element (HRE), activated gluco...

journal_title：Steroids

authors： Grossmann C,Ruhs S,Seiferth A,Gekle M

更新日期：2010-08-01 00:00:00

abstract：:Human placental estradiol 17 beta-dehydrogenase (E.C. 1.1.1.62) was inactivated at pH 6.3 by 3-bromo [2'-14C] acetoxy-1,3,5(10) estratrien-17-one, a know substrate. The affinity-alkylated enzyme was then hydrolyzed by trypsin. Radioactive peptides were initially isolated by gel filtration and identified according to w...

journal_title：Steroids

authors： Murdock GL,Warren JC

更新日期：1982-02-01 00:00:00

abstract：:We recently characterized the awakening responses and daily profiles of the catabolic stress hormone cortisol in elite military men. Anabolic hormones follow a similar daily pattern and may counteract the catabolic effects of cortisol. This companion report is the first to characterize daily profiles of anabolic hormo...

journal_title：Steroids

authors： Taylor MK,Kviatkovsky SA,Hernández LM,Sargent P,Segal S,Granger DA

更新日期：2016-06-01 00:00:00

abstract：:The syndrome of apparent mineralocorticoid excess (AME) is an inherited form of hypertension in which 11 beta-hydroxysteroid dehydrogenase (11-HSD) is defective. This enzyme converts cortisol to its inactive metabolite, cortisone. The deficiency allows mineralocorticoid receptors to be occupied by cortisol, because th...

journal_title：Steroids

authors： White PC,Mune T,Rogerson FM,Kayes KM,Agarwal AK

更新日期：1997-01-01 00:00:00

abstract：:Recent studies have suggested that progestins play a role in the etiology of breast cancer; however, the mechanisms by which progestins promote tumor formation/progression have not been defined. Progestin action, in target tissues such as the breast, is mediated by the progesterone receptor (PR). PR signaling is compl...

journal_title：Steroids

authors： Treviño LS,Bingman WE 3rd,Edwards DP,Nl W

更新日期：2013-06-01 00:00:00

abstract：:Trilostane is a competitive inhibitor of 3beta-hydroxysteroid dehydrogenase. In vitro, the drug inhibits conversion of pregnenolone to progesterone but does not alter conversion of cholesterol to pregnenolone nor progesterone to corticoid hormones. When given orally to rats, trilostane inhibits corticosterone and aldo...

journal_title：Steroids

authors： Potts GO,Creange JE,Hardomg HR,Schane HP

更新日期：1978-09-01 00:00:00