Abstract：
:The formation of a horseradish peroxidase-testosterone conjugate for the enzyme-linked immunoassay of testosterone was investigated, using tritiated testosterone to follow the reaction. The formation of testosterone-3-(carboxymethyl) oxime-peroxidase by the mixed anhydride method was found to give a conjugate of high enzymatic activity and with three molecules of testosterone per molecule of peroxidase. The optimum conditions for the assay of peroxidase activity were studied and an assay capable of measuring 1 to 5 ng of the conjugate developed; the standard curve being virtually linear. The stability of the conjugate in solution and the effect of lyophilisation on enzymatic activity are also described. The peroxidase-testosterone conjugate was suitable for enzyme-linked immunoassay and the quantities measurable with the peroxidase assay covered the range necessary for a plasma testosterone assay. The stability of the conjugate was such that no particular precautions were necessary for its storage.
journal_name
Steroidsjournal_title
Steroidsauthors
Rajkowski KM,Cittanova N,Desfosses B,Jayle MFdoi
10.1016/0039-128x(77)90021-6subject
Has Abstractpub_date
1977-05-01 00:00:00pages
701-13issue
5eissn
0039-128Xissn
1878-5867pii
0039-128X(77)90021-6journal_volume
29pub_type
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