Abstract：
:We have investigated the metabolism of deoxycorticosterone in A6 cells, a continuous cell line derived from the kidney of Xenopus laevis. A6 cells at confluence were incubated with serum-free culture media containing 2.5 microM [3H] deoxycorticosterone. When radioactive compounds in incubation media were separated by reverse-phase high-pressure liquid chromatography, the formation of polar metabolites was observed. One component of polar metabolites cochromatographed with 6 beta-hydroxydeoxycorticosterone. In order to identify this component more rigorously, large scale cultures were performed and this compound was separated and purified by reverse-phase high-pressure liquid chromatography. The purified material was analyzed by gas chromatography-mass spectrometry, secondary ion mass spectrometry, and nuclear magnetic resonance spectroscopy. These procedures revealed that this material was 6 beta-hydroxydeoxycorticosterone.
journal_name
Steroidsjournal_title
Steroidsauthors
Matsuzaki K,Arai T,Miyazaki T,Yasuda Kdoi
10.1016/0039-128x(95)00038-rsubject
Has Abstractpub_date
1995-07-01 00:00:00pages
457-62issue
7eissn
0039-128Xissn
1878-5867pii
0039-128X(95)00038-Rjournal_volume
60pub_type
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