Abstract:
:Gonadal steroids modulate adrenal gland size and function in a variety of species, and our previous studies demonstrate that circulating androgens suppress 3beta-hydroxysteroid dehydrogenase-isomerase (3betaHSD) activity in the adrenal cortex of male mice. The present study tests the hypothesis that androgens have a direct, receptor-mediated inhibitory effect on adrenal 3betaHSD. Treatment of cultured adrenal cells from C57BL/6J and C3H/HeJ mice with 0.02-2.0 microM testosterone for 7 days significantly reduces 3betaHSD activity in cells from both strains. However, treatment for 3 days reduces 3betaHSD activity in the adrenal cells from C3H/HeJ, but not C57BL/6J mice. The decreases in 3betaHSD activity in response to testosterone treatment is reflected in decreases in the amount of 3betaHSD immunoreactive protein, such that extended treatment decreases 3betaHSD immunoreactive protein in adrenal cells from both strains, but short-term treatment only decreases 3betaHSD immunoreactive protein in adrenal cells from C3H/HeJ mice. Thus, there appears to be a temporal difference between strains in the effect of the testosterone on 3betaHSD activity and immunoreactive protein. Treatment of the adrenal cells with androgen agonists and an antagonist indicate that the effect of testosterone is androgen receptor mediated. The effect of testosterone appears to be specific for 3betaHSD, since none of the treatments alter P450(scc) in cells from either strain. Testosterone treatment also causes a decrease in the amount of 3betaHSD mRNA. However, in contrast to the effect on activity and immunoreactive protein, there is no strain-related temporal difference because testosterone decreases 3betaHSD mRNA within 24h in adrenal cells from both strains. These results indicate that testosterone can act directly on the adrenal gland to decrease 3betaHSD activity, immunoreactive protein, and mRNA content in mouse adrenal glands, and thus contribute to the sex difference in adrenal function observed in many species.
journal_name
Steroidsjournal_title
Steroidsauthors
Stalvey JRdoi
10.1016/s0039-128x(02)00023-5subject
Has Abstractpub_date
2002-07-01 00:00:00pages
721-31issue
8eissn
0039-128Xissn
1878-5867pii
S0039128X02000235journal_volume
67pub_type
杂志文章相关文献
STEROIDS文献大全abstract::The relationship between 14CO2 evolution from the catabolism of [26 or 2714C] cholesterol to bile acids was studied in rats with biliary fistulae. When equal quantities of [26 or 2714C] cholesterol and [414C] cholesterol were administered, there was a significant linear relationship between 14CO2 expiration in the bre...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(75)90062-8
更新日期:1975-10-01 00:00:00
abstract::Dioscorea panthaica is a traditional Chinese medicinal herb used in the treatment of various physiological conditions, including cardiovascular disease, gastropathy and hypertension. Steroidal saponins (SS) are the main active ingredients of this herb and have effects on myocardial ischemia and cancer. The phytochemic...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2014.12.023
更新日期:2015-03-01 00:00:00
abstract::A technique of incubation of testicular tissue in vitro with radiolabeled precursors was applied in the investigation of the steroid biosynthesis by testes of four young men after long-term, high-dose estrogen treatment. A positive correlation between plasma and testicular steroid levels, and in vitro capacity of the ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(77)90121-0
更新日期:1977-06-01 00:00:00
abstract::A first assay based on stable isotope dilution/gas chromatography-mass spectrometry (ID/GC-MS) has been developed for plasma 11-deoxycortisol (Reichstein's compound S), the leading hormonal marker of 11beta-hydroxylase deficiency. A suitable internal standard being unavailable, we synthesized dideuterated 11-deoxycort...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(02)00052-1
更新日期:2002-09-01 00:00:00
abstract::3 beta-Hydroxy-5-androsten-17-one is converted to 5-androstene-3, 17-dione by rat liver alcohol dehydrogenase (ADH). We have reported on the purity of the enzyme which is eluted with pyrazole as a single homogeneous protein using an AMP-agarose affinity column. Rat liver ADH can oxidize hydroxyl groups not only at 3 b...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(82)90079-4
更新日期:1982-11-01 00:00:00
abstract::Fermentation of mesterolone (1) with Cunninghamella blakesleeana yielded four new metabolites, 1α-methyl-1β,11β,17β-trihydroxy-5α-androstan-3-one (2), 1α-methyl-7α,11β,17β-trihydroxy-5α-androstan-3-one (3), 1α-methyl-1β,6α,17β-trihydroxy-5α-androstan-3-one (4) and 1α-methyl-1β,11α,17β-trihydroxy-5α-androstan-3-one (5)...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2014.01.001
更新日期:2014-04-01 00:00:00
abstract::Carbon-11-labeled casimiroin analogues were first designed and synthesized as new potential PET agents for imaging of quinone reductase (QR) 2 and aromatase expression in breast cancer. [(11)C]casimiroin (6-[(11)C]methoxy-9-methyl-[1,3]dioxolo[4,5-h]quinolin-8(9H)-one, [(11)C]11) and its carbon-11-labeled analogues 5,...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2010.06.004
更新日期:2010-12-01 00:00:00
abstract::Novel deoxycholic acid (DCA) derivatives were stereoselectively synthesised with -OH and -CH2SR moieties at the C-3 position, where R was a substituted aryl [2-aminophenyl (8) or 4-chlorophenyl (9)] or hetaryl [1-methylimidazolyl (5), 1,2,4-triazolyl (6), 5-amino-1,3,4-thiadiazolyl (7), pyridinyl (10) or pyrimidinyl (...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2017.08.016
更新日期:2017-11-01 00:00:00
abstract::This study represents a continuing effort to find a new biomarker for the diagnosis and management of prostatic cancer. Polyclonal antibodies were prepared to a peptide (CAKP) representing amino acids 28 to 43 of the 5 alpha-reductase type 2 isozyme. Using immunoaffinity-purified antibodies, the sera of 62 patients we...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(96)00184-5
更新日期:1996-11-01 00:00:00
abstract::The purpose of this study was to investigate the influence of plasma steroid-binding proteins on androgen metabolism in intact leukocytes prepared from normal male and female blood samples. Leukocyte preparations were incubated for 24 h at 37 degrees C with either labeled or unlabeled testosterone (T), 5 alpha-dihydro...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(94)00042-b
更新日期:1995-02-01 00:00:00
abstract::The nature of the water-soluble products formed by incubating labelled estradiol with uterine peroxidase in the presence of H2O2 and tyrosine was examined by two-dimensional thin-layer chromatography and high voltage electrophoresis. It was shown that the steroid and amino acid were associated in a 1:2 or 1:3 ratio an...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(77)90072-1
更新日期:1977-04-01 00:00:00
abstract::To investigate the effects of conjugated bile acid on bacterial growth and cholylglycine hydrolase activity, Clostridium perfringens from human feces was exposed to varying concentrations of taurine- or glycine-conjugated chenodeoxycholic acid. Extracellular enzyme activity was determined by deconjugation of radiolabe...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(94)90062-0
更新日期:1994-08-01 00:00:00
abstract::The high concentrations of dehydroepiandrosterone sulfate and pregnenolone sulfate in the mammalian brain, despite the blood-brain barrier's impermeability to these compounds, and the apparent independence of these concentrations from those in plasma prompted us to investigate whether enzymatic sulfation of dehydroepi...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(97)00013-5
更新日期:1997-05-01 00:00:00
abstract::Dehydroepiandrosterone (DHEA) reduces body fat in rodents and humans, and increases glycerol release from isolated rat epididymal adipocytes and human visceral adipose tissue explants. It suggests that DHEA stimulates triglyceride hydrolysis in adipose tissue; however, the mechanisms underlying this action are still u...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2012.08.002
更新日期:2012-11-01 00:00:00
abstract::The four diastereomeric 3,16-diacetates 6, 9, 10 and 11 were prepared from gitoxin 1 by the routes shown in Schemes 1 and 2 and tested for inotropic activity in the isolated guinea-pig atrial preparation. In line with earlier findings in the digitoxigenin series, derivatives with a 3 alpha-acetoxy function, viz 9 and ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(85)90062-5
更新日期:1985-01-01 00:00:00
abstract::The efficiencies for estrogen conjugate hydrolysis were compared between enzyme hydrolysis, acid solvolysis and a new method, ammonolysis. Samples included: 1) crystalline 1,3,5(10)-estratriene-3, 17 beta-diol disulfate (estradiol 3,17-disulfate), 2) squirrel monkey urine collected following an intravenous injection o...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(84)90023-0
更新日期:1984-06-01 00:00:00
abstract::The metabolism of a C26 bile alcohol (I, 24-nor-5beta-cho-lestane-3alpha, 7alpha,25-triol) was studied in the isolated perfused rabbit liver. The new bile alcohol and bile acid metabolites secreted into the bile were isolated and identified by a combination of TLC, GLC and GLC-MS. The following bile alcohols were foun...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(77)90099-x
更新日期:1977-10-01 00:00:00
abstract::The chemoselectivity of rigid cyclic alpha,beta-unsaturated carbonyl group on the reducing agents was influenced by the ring size and steric factor. Cholesterol (cholest-5-en-3beta-ol) and dehydroepiandrosterone (DHEA) were oxidized with 2,3-dichloro-5,6-dicyano-1,4-benzoquinone to form 1,4,6-cholestatrien-3-one and 1...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2006.12.008
更新日期:2007-04-01 00:00:00
abstract::CGS 16949A inhibited the conversion of [4-14C]androstenedione (A) to [4-14C]estrone by human placental microsomes in a competitive manner (Ki = 1.6 nM). Aminoglutethimide, also a competitive inhibitor, had a Ki = 0.7 microM in this assay system. The Km for the aromatization of A was 0.11 microM. Using ovarian microsom...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(83)90068-5
更新日期:1987-07-01 00:00:00
abstract::A radioimmunoassay (RIA) method is described for the determination of 4-androstene-3, 11, 17-trione (11-oxo-androstenedione) in human plasma. 4-androstene-3, 11, 17-trione 3-(0-carboxymethyl) oxime-bovine serum albumin conjugate was used to generate highly specific antiserum in rabbits. Cross reactivities of several o...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(84)80013-6
更新日期:1984-07-01 00:00:00
abstract:OBJECTIVE:To evaluate the role of physiologic levels of androgens and their precursors in the regulation of body composition, energy and substrate metabolism and aerobic capacity in healthy, cycling, premenopausal women. EXPERIMENTAL:We evaluated 30 young (27±1 year) premenopausal, non-obese (23±0.5 kg/m(2)), normally...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2011.06.001
更新日期:2011-11-01 00:00:00
abstract::A facile method for the synthesis of a series of new steroidal 3-thioxamides from the 3-oxo compound, variously substituted at the 17 position, is described. The "one pot" reaction, using Lawesson's reagent (4-methoxyphenylthionophosphine sulphide dimer) in dichloromethane solution, gives the desired compounds with a ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(97)00003-2
更新日期:1997-06-01 00:00:00
abstract::Sertoli cells isolated from 17 day old rats were maintained in culture and incubated with [14C]-progesterone for 20 h. The cells and media were extracted with ether/chloroform and the extracts chromatographed two-dimensionally on TLC and the radioactive metabolites visualized by autoradiography. Nine of the metabolite...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(80)80009-2
更新日期:1980-05-01 00:00:00
abstract::A trisulfated derivative of 24,25,26,26-tetramethyl-5 alpha-cholest-23E-ene-2 alpha, 3 beta, 6 alpha-triol (sokotrasterol sulfate) has been isolated from the sponge Halichondriidae gen. sp., collected near Sokotra Island (Arabian Sea), and its structure has been elucidated. The side chain of the new steroid involves a...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(83)90039-9
更新日期:1983-09-01 00:00:00
abstract::We report the synthesis of 3 beta,15 beta,17 alpha-trihydroxy-5-pregnen-20- one (1) from 3 beta,15 beta-dihydroxy-5,16-pregnadien-20-one (11) in 7 steps using boronate derivatives as a means of protecting the 17,20-glycol side-chain of steroid intermediates. 16 alpha,17 alpha-Epoxy-3 beta,15 beta-dihydroxy-5- pregnen-...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(95)00169-q
更新日期:1996-01-01 00:00:00
abstract::A series of steroidal and nonsteroidal Michael acceptors that represent reaction products for 3 alpha-hydroxysteroid dehydrogenase were synthesized and evaluated as potential enzyme-generated inactivators. Introduction of exocyclic olefins either at C-2 or C-6 produced inhibitors with high affinity for the enzyme (0.0...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(91)90030-y
更新日期:1991-08-01 00:00:00
abstract::Prostaglandin E(2) (PGE(2)) is a major mediator in the pathophysiology, and pathogenesis of gynecological diseases associated with abnormal endometrial disease with proliferation and inflammation, such as endometriosis. In this study, we investigated the effect of dienogest, a selective progesterone receptor agonist, ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2010.08.010
更新日期:2011-01-01 00:00:00
abstract::Progestagens inhibit growth of endometrial cancer cells in vivo and in vitro, and also are reported to inhibit endometrial cancer cell invasion. The progesterone receptor (PR) isotypes PRA and PRB have different transcriptional activity. There are indications that relative over expression of PRB could lead to developm...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2003.08.002
更新日期:2003-11-01 00:00:00
abstract::The concentration of testosterone (T) in the peripheral plasma of laboratory mice is extremely variable. This variability is already evident at 20--25 days of age and is not eliminated by brief or chronic exposure to male or female mice, or by isolation. The variation in T levels in plasma samples collected from the s...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(75)90107-5
更新日期:1975-12-01 00:00:00
abstract::Initial excretion studies with orally administered [monoethyl-1-3H] DES demonstrated the feces to be the principal mode of elimination of DES in the C3H mouse. Metabolic studies with tritiated DES and/or [UL-14C] DES were performed with orally dosed C3H high (MTV+) and low (MTV-) titer MMTV female mice. Extraction and...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(78)90029-6
更新日期:1978-04-01 00:00:00