Abstract:
:Glucocorticoids are well-recognized modulators of immunocytes and osteoblasts via specific receptor-mediated mechanisms. We have evaluated the in vitro effect of interleukin 2 (IL-2) on the expression of glucocorticoid receptors (GRs) in peripheral blood mononuclear cells (PBMCs) obtained from healthy donors and osteoblast-like Saos-2 cells. Aliquots of PBMC or Saos-2 cells were incubated for 20 h in the presence or absence of recombinant human IL-2 (100 IU/mL) at 37 degrees C. After incubation, a [3H]dexamethasone radioligand-binding assay and Scatchard analysis were used to determine GR-binding parameters in both cell populations. Saos-2 cells basally express higher numbers of GR than PBMCs. After IL-2, a significant increase in GR number was found for both PBMCs and Saos-2 cells. The relative increase was higher in Saos-2 cells; in PBMCs, the apparent affinity fell to almost half. These data represent an additional piece of evidence that cytokine and steroid hormones may act in a complementary way to regulate specific cell functions.
journal_name
Steroidsjournal_title
Steroidsauthors
Sartori ML,Masera RG,Staurenghi A,Racca S,Angeli Adoi
10.1016/s0039-128x(98)00023-3subject
Has Abstractpub_date
1998-05-01 00:00:00pages
349-51issue
5-6eissn
0039-128Xissn
1878-5867pii
S0039-128X(98)00023-3journal_volume
63pub_type
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