Abstract:
:Addition of soluble supernatant to testis microsomes results in 42% increase in steroid 17,20-lyase activity and a 65% increase in 17alpha-hydroxylase activity. This stimulatory activity could be partially purified by salt fractionation. The activating factor(s) was not removed by dialysis nor did it appear to be lipid. It was destroyed by trypsin. Differential effects of heat were observed with the hydroxylase and lyase activators. The activation did not affect Km but only increased Vmax. The supernatant could be added to each enzyme to the point of maturation. No binding of steroids by the supernatant could be detected. Corpus luteum and placental supernatant did not stimulate enzymic activity, but supernatant from an adrenal adenoma was active.
journal_name
Steroidsjournal_title
Steroidsauthors
Betz G,Tsai Pdoi
10.1016/0039-128x(78)90090-9subject
Has Abstractpub_date
1978-10-01 00:00:00pages
389-97issue
3eissn
0039-128Xissn
1878-5867pii
0039-128X(78)90090-9journal_volume
32pub_type
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