Allopregnanolone promotes proliferation and differential gene expression in human glioblastoma cells.


:Allopregnanolone (3α-THP) is one of the main reduced progesterone (P4) metabolites that is recognized as a neuroprotective and myelinating agent. 3α-THP also induces proliferation of different neural cells. It has been shown that P4 favors the progression of glioblastomas (GBM), the most common and aggressive primary brain tumors. However, the role of 3α-THP in the growth of GBMs is unknown. Here, we studied the effects of 3α-THP on the number of cells, proliferation and gene expression in U87 cell line derived from a human GBM. 3α-THP (10, 100nM and 1μM) increased the number of U87 cells, and at 10nM exerted a similar increase in both the number of total and proliferative U87 cells as compared with P4 (10nM). Interestingly, finasteride (F; 100nM), an inhibitor of 5α-reductase (5αR), an enzyme necessary to metabolize P4 and produce 3α-THP, blocked the increase in the number of U87 cells induced by P4. By using RT-qPCR, we determined that U87 cells express 5α-R isoenzymes 1 and 2 (5αR1 and 5αR2), being 5αR1 the predominant one in these cells. 3α-THP (10nM) increased the expression of TGFβ1, EGFR, VEGF and cyclin D1 genes. P4 increased TGFβ1 and EGFR expression, and this effect was blocked by F. These data provide evidence that P4, through its metabolite 3α-THP, can promote in part cell proliferation of human GBM cells by changing the expression of genes involved in tumor progression.






Zamora-Sánchez CJ,Hansberg-Pastor V,Salido-Guadarrama I,Rodríguez-Dorantes M,Camacho-Arroyo I




Has Abstract


2017-03-01 00:00:00














  • Rat and human embryo and post-natal sera contain a potent endogenous competitor of estrogen-rat alpha-fetoprotein interactions.

    abstract::A highly active inhibitor of the binding of estrone and estradiol-17beta to rat alpha-fetoprotein is demonstrated for the first time in embryo, immature and adult rat sera as well as in fetal and adult human sera. The competitive character and the narrow specificity of this inhibition effect is shown. The major compou...


    pub_type: 杂志文章


    authors: Benassayag C,Vallette G,Delorme J,Savu L,Nunez EA,Jayle MF

    更新日期:1977-12-01 00:00:00

  • Determination of endocrine disruptors and endogenic androgens and estrogens in rat serum by high-performance liquid chromatography-tandem mass spectrometry.

    abstract::To simultaneously measure some targeted endocrine disruptors and several forms of sex hormones in rat serum, an accurate analytical procedure was developed. First, a comparison between a polymeric-based solid-phase extraction (SPE) and a micro-extraction by packed sorbent was performed to choose the optimal method to ...


    pub_type: 杂志文章


    authors: Tournier M,Pouech C,Quignot N,Lafay F,Wiest L,Lemazurier E,Cren-Olivé C,Vulliet E

    更新日期:2015-12-01 00:00:00

  • Peripheral benzodiazepine receptor in cholesterol transport and steroidogenesis.

    abstract::Steroidogenesis begins with the metabolism of cholesterol to pregnenolone by the inner mitochondrial membrane cytochrome P450 side-chain cleavage (P450scc) enzyme. The rate of steroid formation, however, depends on the rate of cholesterol transport from intracellular stores to the inner mitochondrial membrane and load...


    pub_type: 杂志文章,评审


    authors: Papadopoulos V,Amri H,Boujrad N,Cascio C,Culty M,Garnier M,Hardwick M,Li H,Vidic B,Brown AS,Reversa JL,Bernassau JM,Drieu K

    更新日期:1997-01-01 00:00:00

  • Estradiol and the control of feeding behavior.

    abstract::This review lays out the evidence for the role of E2 in homeostatic and hedonic feeding across several species. While significant effort has been expended on homeostatic feeding research, more studies for hedonic feeding need to be conducted (i.e. are there increases in meal size and enhanced motivation to natural foo...


    pub_type: 杂志文章,评审


    authors: Rivera HM,Stincic TL

    更新日期:2018-05-01 00:00:00

  • Molecular analysis of congenital adrenal hyperplasia due to 21-hydroxylase deficiency in Hong Kong Chinese patients.

    abstract:BACKGROUND:Congenital adrenal hyperplasia (CAH) caused by 21-hydroxylase deficiency (21OHD) is an autosomal recessive disorder due to mutation in the CYP21A2 gene. OBJECTIVE:To elucidate the genetic basis of 21-hydroxylase-deficient CAH in Hong Kong Chinese patients. PATIENTS AND METHODS:Mutational analysis of the CY...


    pub_type: 杂志文章


    authors: Chan AO,But WM,Ng KL,Wong LM,Lam YY,Tiu SC,Lee KF,Lee CY,Loung PY,Berry IR,Brown R,Charlton R,Cheng CW,Ho YC,Tse WY,Shek CC

    更新日期:2011-09-01 00:00:00

  • Carbenoxolone does not cause a syndrome of mineralocorticoid excess in sheep.

    abstract::These studies investigated whether treatment with carbenoxolone (CBX), an inhibitor of 11 beta-hydroxysteroid dehydrogenase (11 beta-HSD), resulted in an enhanced mineralocorticoid response to endogenous or infused cortisol. In conscious sodium replete sheep with a parotid fistula, infusion of CBX (40 mg/h for 10 days...


    pub_type: 杂志文章


    authors: Dodic M,May CN,Coghlan JP

    更新日期:1998-02-01 00:00:00

  • Synthesis of androstene oxime-nitrogen mustard bioconjugates as potent antineoplastic agents.

    abstract::In the present study, synthesis and antineoplastic activity of phenylacetic acid and benzoic acid nitrogen mustard conjugates of various steroidal oximes are reported for the first time. The conjugation was achieved through a more stable oxime-ester linkage and the resulting newly synthesized conjugates were evaluated...


    pub_type: 杂志文章


    authors: Acharya PC,Bansal R

    更新日期:2017-07-01 00:00:00

  • Repeat estradiol exposure differentially regulates protein expression patterns for estrogen receptor and E-cadherin in older mouse ovarian surface epithelium: implications for replacement and adjuvant hormone therapies?

    abstract:BACKGROUND:Estrogen replacement therapy increases risk for ovarian epithelial cancer, a cancer of mainly older women, yet the response of older ovarian surface epithelium (OSE) to repeat estrogen exposure overtime has not been studied. We have previously reported significant reductions in estrogen receptor (ER) protein...


    pub_type: 杂志文章


    authors: Gulliver LS,Hurst PR

    更新日期:2012-05-01 00:00:00

  • Individual and cyclic estrogenic profile in women: Structure and variability of the data.

    abstract::The concentration of estrogens in the body fluids of women is highly variable, due to the menstrual cycle, circadian oscillations, and other physiological and pathological causes. To date, only the cyclic fluctuations of the principal estrogens (estradiol and estrone) have been studied, with limited outcome of general...


    pub_type: 杂志文章


    authors: Bozzolino C,Vaglio S,Amante E,Alladio E,Gerace E,Salomone A,Vincenti M

    更新日期:2019-10-01 00:00:00

  • Expression of membrane progesterone receptors (mPRs) in rat peripheral glial cell membranes and their potential role in the modulation of cell migration and protein expression.

    abstract::The role played by progestogens in modulating Schwann cell pathophysiology is well established. Progestogens exert their effects in these cells through both classical genomic and non-genomic mechanisms, the latter mediated by the GABA-A receptor. However, there is evidence that other receptors may be involved. Membran...


    pub_type: 杂志文章


    authors: Castelnovo LF,Magnaghi V,Thomas P

    更新日期:2019-02-01 00:00:00

  • Dehydroepiandrosterone fatty acyl esters in high density lipoprotein: interaction with human vascular endothelial cells and vascular responses ex vivo.

    abstract::Dehydroepiandrosterone (DHEA) fatty acyl esters once incorporated in high density lipoprotein (HDL) induce a stronger vasodilatory response in rat mesenteric arteries ex vivo compared to native HDL. We studied the role of HDL receptor, scavenger receptor class B, type 1 (SR-B1), as well as estrogen and androgen recept...


    pub_type: 杂志文章


    authors: Paatela H,Vihma V,Jauhiainen M,Mervaala E,Tikkanen MJ

    更新日期:2011-03-01 00:00:00

  • Comparative aspects of 11 beta-hydroxysteroid dehydrogenase. Testicular 11 beta-hydroxysteroid dehydrogenase: development of a model for the mediation of Leydig cell function by corticosteroids.

    abstract::It has been shown that stress or disease-induced increases in plasma corticosterone result in diminished testosterone secretion from the testes. This article reviews investigations from our laboratories that explore the role of 11 beta-hydroxysteroid dehydrogenase (11 beta-OHSD) in this process. It is proposed that th...


    pub_type: 杂志文章,评审


    authors: Monder C,Hardy MP,Blanchard RJ,Blanchard DC

    更新日期:1994-02-01 00:00:00

  • Glucocorticosteroids as antioxidants in treatment of asthma and COPD. New application for an old medication?

    abstract::Inhaled corticosteroids (ICS) are the standard of care in asthma and are widely used in the treatment of patients with COPD. The influence of steroids on inflammatory processes has long been established since glucocorticoids and their receptor belong to the regulatory network involved in inhibition of several inflamma...


    pub_type: 杂志文章,评审


    authors: Sadowska AM,Klebe B,Germonpré P,De Backer WA

    更新日期:2007-01-01 00:00:00

  • An approach towards the development of progesterone antagonists: synthesis of 7 alpha/7 beta-aryl androstene derivatives.

    abstract::Synthesis of 3 beta,17 beta-dihydroxy-7 alpha/7 beta-(4-hydroxyphenyl)-androst-5-ene 3,17-diacetate (4 and 5, R = H) and 3 beta,17 beta-dihydroxy-7 alpha/7 beta-(4-methoxyphenyl)-androst-5-ene 3,17-diacetate (4 and 5, R = Me) have been carried out by Friedel-Crafts reaction on 3 beta,7,17 beta-trihydroxy-androst-5-ene...


    pub_type: 杂志文章


    authors: Negi AS,Dwivedy I,Roy R,Ray S

    更新日期:1995-06-01 00:00:00

  • Binding characteristics of [3H]delta(5)-androstene-3beta,17beta-diol to a nuclear protein in the rabbit vagina.

    abstract::In this study, we investigated the binding characteristics of [3H]Delta(5)-androstene-3beta,17beta-diol to rabbit vaginal cytosolic and nuclear extracts and in freshly excised intact tissue strips. [3H]delta(5)-Androstene-3beta,17beta-diol bound to a protein(s) in the vaginal nuclear extract with high affinity (K(d)=3...


    pub_type: 杂志文章


    authors: Traish AM,Huang YH,Min K,Kim NN,Munarriz R,Goldstein I

    更新日期:2004-01-01 00:00:00

  • Rapid inhibitory effect of glucocorticoids on airway smooth muscle contractions in guinea pigs.

    abstract::The common disease asthma is characterized by the obstruction, inflammation and increased sensitivity of the airways. Glucocorticoids (GCs) are one of the most potent anti-inflammatory agents available for treating allergic disease. In this study, we report that the GC budesonide (BUD) can rapidly inhibit the histamin...


    pub_type: 杂志文章


    authors: Sun HW,Miao CY,Liu L,Zhou J,Su DF,Wang YX,Jiang CL

    更新日期:2006-02-01 00:00:00

  • Differences in temperature-sensitive receptor binding of glucocorticoids in spontaneously hypertensive and normotensive Wistar-Kyoto rats.

    abstract::Glucocorticoid receptor binding was compared in liver cytosol preparations from spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats using homologous displacement of [3H]dexamethasone. At 5 degrees C, there was no difference in receptor binding affinity or concentration between strains for dexamethasone, ...


    pub_type: 杂志文章


    authors: Panarelli M,Holloway CD,Barr AB,Fraser R,Kenyon CJ

    更新日期:1995-01-01 00:00:00

  • Formation and isolation of delta 5-3-ketosteroids using a purified rat liver alcohol dehydrogenase.

    abstract::3 beta-Hydroxy-5-androsten-17-one is converted to 5-androstene-3, 17-dione by rat liver alcohol dehydrogenase (ADH). We have reported on the purity of the enzyme which is eluted with pyrazole as a single homogeneous protein using an AMP-agarose affinity column. Rat liver ADH can oxidize hydroxyl groups not only at 3 b...


    pub_type: 杂志文章


    authors: Knutson VP,Ungar F

    更新日期:1982-11-01 00:00:00

  • Inhibition of progesterone synthesis in normal and transformed human placental cells by tight binding inhibitors of 3 beta-hydroxysteroid dehydrogenase.

    abstract::The biosynthesis of progesterone from [3H]pregnenolone was curvilinear over a 6 h time course in human placenta cytotrophoblasts and in human placenta choriocarcinoma cells (JEG-3 cells). Mass measurements determined independently by radioimmunoassay indicate that the progesterone synthesized by cytotrophoblasts (21.0...


    pub_type: 杂志文章


    authors: Sharp RB,Penning TM

    更新日期:1988-05-01 00:00:00

  • Steroidal alkenylphosphonates via palladium-catalyzed coupling reactions.

    abstract::The palladium-catalyzed coupling of various 17-iodo-delta 16 steroids (17-iodo-androst-16-ene, 17-iodo-4-methyl-4-aza-androst-16-en-3-one, and 17-iodo-4-aza-androst-16-en-3-one) with dialkyl phosphites (dimethyl phosphite, diethyl phosphite, and diisopropyl phosphite) was examined in detail. The only successful condit...


    pub_type: 杂志文章


    authors: Skoda-Földes R,Kollár L,Horváth J,Tuba Z

    更新日期:1995-12-01 00:00:00

  • Brassinosteroids application induces phosphatidic acid production and modify antioxidant enzymes activity in tobacco in calcium-dependent manner.

    abstract::Brassinosteroids (BRs) are steroid hormones regulating various aspects of plant metabolism, including growth, development and stress responses. However, little is known about the mechanism of their impact on antioxidant systems and phospholipid turnover. Using tobacco plants overexpressing H+/Ca2+vacuolar Arabidopsis ...


    pub_type: 杂志文章


    authors: Kretynin SV,Kolesnikov YS,Derevyanchuk MV,Kalachova TA,Blume YB,Khripach VA,Kravets VS

    更新日期:2019-07-08 00:00:00

  • A novel synthetic approach to (20R)- and (20S)-21-hydroxy steroids. Synthesis of a marine sterol 21-hydroxycholesterol.

    abstract::A short and efficient synthesis of steroid synthons, di(tert-butyldimethylsilyl) ethers of 3,21-dihydroxy-24-nor-chol-5-en-23-al (8 and 10) and of ethyl 3,21-dihydroxy-25-homo-chola-5,23-dien-25-oate (9 and 11), having natural (20R) and unnatural (20S) configuration from 3β-(tert-butyldimethylsilyloxy)-14α,20ξ-card-5-...


    pub_type: 杂志文章


    authors: Koenig H,Skiera I,Błaszczyk K,Paryzek Z

    更新日期:2011-04-01 00:00:00

  • Caveolin-Na/K-ATPase interactions: role of transmembrane topology in non-genomic steroid signal transduction.

    abstract::Progesterone and its polar metabolite(s) trigger the meiotic divisions in the amphibian oocyte through a non-genomic signaling system at the plasma membrane. Published site-directed mutagenesis studies of ouabain binding and progesterone-ouabain competition studies indicate that progesterone binds to a 23 amino acid e...


    pub_type: 杂志文章


    authors: Morrill GA,Kostellow AB,Askari A

    更新日期:2012-09-01 00:00:00

  • Major cardiac surgery induces an increase in sex steroids in prepubertal children.

    abstract::While the neuroprotective benefits of estrogen and progesterone in critical illness are well established, the data regarding the effects of androgens are conflicting. Surgical repair of congenital heart disease is associated with significant morbidity and mortality, but there are scant data regarding the postoperative...


    pub_type: 杂志文章


    authors: Heckmann M,d'Uscio CH,de Laffolie J,Neuhaeuser C,Bödeker RH,Thul J,Schranz D,Frey BM

    更新日期:2014-03-01 00:00:00

  • 5alpha-reduction of an anti-androgen TSAA-291, 16beta-ethyl-17beta-hydroxy-4-estren-3-one, by nuclear 5alpha-reductase in rat prostates.

    abstract::Inhibition of 5alpha-reduction of testosterone by an anti-androgen TSAA-291 (16beta-ethyl-17beta-hydroxy-4-estren-3-one) was studied in rat ventral prostates and the metabolic conversion of 3H-TSAA-291 was examined both in vitro and in vivo. In the in vitro experiment using nuclear 5alpha-reductase of the prostate, 5a...


    pub_type: 杂志文章


    authors: Sudo K,Yoshida K,Akinaga Y,Nakayama R

    更新日期:1981-07-01 00:00:00

  • Chromatographic patterns of urinary ethynyl estrogen metabolites in various populations.

    abstract::Radioactive mestranol (ME) and/or ethynylestradiol (EE) were administered to women in Nigeria, Sri Lanka, and the USA, and the types and patterns of radioactive urinary conjugates examined by Sephadex LH-20 chromatography. There are no differences in the total excretion of urinary radioactivity over 3 days. Consistent...


    pub_type: 杂志文章


    authors: Williams MC,Goldzieher JW

    更新日期:1980-09-01 00:00:00

  • Hyfraxins A and B, cytotoxic ergostane-type steroid and lanostane triterpenoid glycosides from the invasive ash dieback ascomycete Hymenoscyphus fraxineus.

    abstract::A virulent culture of Hymenoscyphus fraxineus, the causal agent of ash dieback, was investigated for its production of secondary metabolites in a 70 L batch fermentation. Chemical analysis of the mycelial extract by means of flash chromatography and preparative HPLC led to the isolation of a new ergostane-type steroid...


    pub_type: 杂志文章


    authors: Surup F,Halecker S,Nimtz M,Rodrigo S,Schulz B,Steinert M,Stadler M

    更新日期:2018-07-01 00:00:00

  • Evaluation of an EIA method for measuring serum levels of the estrogen metabolite 2-hydroxyestrone in adults.

    abstract::Two-hydroxyestrone (2OHE-1) and 16alpha-hydroxyestrone (16OHE-1) are two estrogen metabolites that may play important roles in the development or promotion of breast cancer. Our study assessed the reliability of a newly developed kit procedure for measuring 2OHE-1. Although under certain conditions the assay would not...


    pub_type: 杂志文章


    authors: Spierto FW,Gardner F,Smith SJ

    更新日期:2001-01-01 00:00:00

  • Elucidation of two new corticosteroids, betamethasone dibutyrate and betamethasone tributyrate.

    abstract::Corticosteroids were used normally as anti-inflammatory drugs. However, in some area certain corticosteroids might be illegally used as growth promoting agent in feed, and as prohibited doping substances in game and sport for human or/and animal performance-enhancing. Synthesized structural similar corticosteroids wer...


    pub_type: 杂志文章


    authors: Chen SY,Fang M,Lin YT,Tsai CF,Cheng HF,Wang DY

    更新日期:2021-01-01 00:00:00

  • Reversible and the irreversible structural alterations on brain after resolution of hypercortisolism in Cushing's disease.

    abstract::The adverse effects of hypercortisolism on the human brain have been highlighted in previous studies of Cushing's disease (CD). However, the reversibility of brain damage after the resolution of hypercortisolism remains unclear. Thus, we studied the potential volumetric reversibility in biochemically remitted CD patie...


    pub_type: 杂志文章


    authors: Jiang H,Liu C,Pan SJ,Ren J,He NY,Sun YH,Bian LG,Yan FH,Yang WJ,Sun QF

    更新日期:2019-11-01 00:00:00