Abstract：
:Allopregnanolone (3α-THP) is one of the main reduced progesterone (P4) metabolites that is recognized as a neuroprotective and myelinating agent. 3α-THP also induces proliferation of different neural cells. It has been shown that P4 favors the progression of glioblastomas (GBM), the most common and aggressive primary brain tumors. However, the role of 3α-THP in the growth of GBMs is unknown. Here, we studied the effects of 3α-THP on the number of cells, proliferation and gene expression in U87 cell line derived from a human GBM. 3α-THP (10, 100nM and 1μM) increased the number of U87 cells, and at 10nM exerted a similar increase in both the number of total and proliferative U87 cells as compared with P4 (10nM). Interestingly, finasteride (F; 100nM), an inhibitor of 5α-reductase (5αR), an enzyme necessary to metabolize P4 and produce 3α-THP, blocked the increase in the number of U87 cells induced by P4. By using RT-qPCR, we determined that U87 cells express 5α-R isoenzymes 1 and 2 (5αR1 and 5αR2), being 5αR1 the predominant one in these cells. 3α-THP (10nM) increased the expression of TGFβ1, EGFR, VEGF and cyclin D1 genes. P4 increased TGFβ1 and EGFR expression, and this effect was blocked by F. These data provide evidence that P4, through its metabolite 3α-THP, can promote in part cell proliferation of human GBM cells by changing the expression of genes involved in tumor progression.
journal_name
Steroidsjournal_title
Steroidsauthors
Zamora-Sánchez CJ,Hansberg-Pastor V,Salido-Guadarrama I,Rodríguez-Dorantes M,Camacho-Arroyo Idoi
10.1016/j.steroids.2017.01.004subject
Has Abstractpub_date
2017-03-01 00:00:00pages
36-42eissn
0039-128Xissn
1878-5867pii
S0039-128X(17)30014-4journal_volume
119pub_type
杂志文章相关文献
STEROIDS文献大全abstract：:A highly active inhibitor of the binding of estrone and estradiol-17beta to rat alpha-fetoprotein is demonstrated for the first time in embryo, immature and adult rat sera as well as in fetal and adult human sera. The competitive character and the narrow specificity of this inhibition effect is shown. The major compou...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/s0039-128x(77)80023-8
更新日期：1977-12-01 00:00:00
abstract：:To simultaneously measure some targeted endocrine disruptors and several forms of sex hormones in rat serum, an accurate analytical procedure was developed. First, a comparison between a polymeric-based solid-phase extraction (SPE) and a micro-extraction by packed sorbent was performed to choose the optimal method to ...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/j.steroids.2015.10.011
更新日期：2015-12-01 00:00:00
abstract：:Steroidogenesis begins with the metabolism of cholesterol to pregnenolone by the inner mitochondrial membrane cytochrome P450 side-chain cleavage (P450scc) enzyme. The rate of steroid formation, however, depends on the rate of cholesterol transport from intracellular stores to the inner mitochondrial membrane and load...
journal_title：Steroids
pub_type： 杂志文章,评审
doi：10.1016/s0039-128x(96)00154-7
更新日期：1997-01-01 00:00:00
abstract：:This review lays out the evidence for the role of E2 in homeostatic and hedonic feeding across several species. While significant effort has been expended on homeostatic feeding research, more studies for hedonic feeding need to be conducted (i.e. are there increases in meal size and enhanced motivation to natural foo...
journal_title：Steroids
pub_type： 杂志文章,评审
doi：10.1016/j.steroids.2017.11.011
更新日期：2018-05-01 00:00:00
abstract：BACKGROUND:Congenital adrenal hyperplasia (CAH) caused by 21-hydroxylase deficiency (21OHD) is an autosomal recessive disorder due to mutation in the CYP21A2 gene. OBJECTIVE:To elucidate the genetic basis of 21-hydroxylase-deficient CAH in Hong Kong Chinese patients. PATIENTS AND METHODS:Mutational analysis of the CY...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/j.steroids.2011.04.010
更新日期：2011-09-01 00:00:00
abstract：:These studies investigated whether treatment with carbenoxolone (CBX), an inhibitor of 11 beta-hydroxysteroid dehydrogenase (11 beta-HSD), resulted in an enhanced mineralocorticoid response to endogenous or infused cortisol. In conscious sodium replete sheep with a parotid fistula, infusion of CBX (40 mg/h for 10 days...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/s0039-128x(97)00141-4
更新日期：1998-02-01 00:00:00
abstract：:In the present study, synthesis and antineoplastic activity of phenylacetic acid and benzoic acid nitrogen mustard conjugates of various steroidal oximes are reported for the first time. The conjugation was achieved through a more stable oxime-ester linkage and the resulting newly synthesized conjugates were evaluated...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/j.steroids.2017.04.005
更新日期：2017-07-01 00:00:00
abstract：BACKGROUND:Estrogen replacement therapy increases risk for ovarian epithelial cancer, a cancer of mainly older women, yet the response of older ovarian surface epithelium (OSE) to repeat estrogen exposure overtime has not been studied. We have previously reported significant reductions in estrogen receptor (ER) protein...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/j.steroids.2012.02.015
更新日期：2012-05-01 00:00:00
abstract：:The concentration of estrogens in the body fluids of women is highly variable, due to the menstrual cycle, circadian oscillations, and other physiological and pathological causes. To date, only the cyclic fluctuations of the principal estrogens (estradiol and estrone) have been studied, with limited outcome of general...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/j.steroids.2019.108432
更新日期：2019-10-01 00:00:00
abstract：:The role played by progestogens in modulating Schwann cell pathophysiology is well established. Progestogens exert their effects in these cells through both classical genomic and non-genomic mechanisms, the latter mediated by the GABA-A receptor. However, there is evidence that other receptors may be involved. Membran...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/j.steroids.2017.09.009
更新日期：2019-02-01 00:00:00
abstract：:Dehydroepiandrosterone (DHEA) fatty acyl esters once incorporated in high density lipoprotein (HDL) induce a stronger vasodilatory response in rat mesenteric arteries ex vivo compared to native HDL. We studied the role of HDL receptor, scavenger receptor class B, type 1 (SR-B1), as well as estrogen and androgen recept...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/j.steroids.2010.12.007
更新日期：2011-03-01 00:00:00
abstract：:It has been shown that stress or disease-induced increases in plasma corticosterone result in diminished testosterone secretion from the testes. This article reviews investigations from our laboratories that explore the role of 11 beta-hydroxysteroid dehydrogenase (11 beta-OHSD) in this process. It is proposed that th...
journal_title：Steroids
pub_type： 杂志文章,评审
doi：10.1016/0039-128x(94)90078-7
更新日期：1994-02-01 00:00:00
abstract：:Inhaled corticosteroids (ICS) are the standard of care in asthma and are widely used in the treatment of patients with COPD. The influence of steroids on inflammatory processes has long been established since glucocorticoids and their receptor belong to the regulatory network involved in inhibition of several inflamma...
journal_title：Steroids
pub_type： 杂志文章,评审
doi：10.1016/j.steroids.2006.10.007
更新日期：2007-01-01 00:00:00
abstract：:Synthesis of 3 beta,17 beta-dihydroxy-7 alpha/7 beta-(4-hydroxyphenyl)-androst-5-ene 3,17-diacetate (4 and 5, R = H) and 3 beta,17 beta-dihydroxy-7 alpha/7 beta-(4-methoxyphenyl)-androst-5-ene 3,17-diacetate (4 and 5, R = Me) have been carried out by Friedel-Crafts reaction on 3 beta,7,17 beta-trihydroxy-androst-5-ene...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/0039-128x(95)00007-d
更新日期：1995-06-01 00:00:00
abstract：:In this study, we investigated the binding characteristics of [3H]Delta(5)-androstene-3beta,17beta-diol to rabbit vaginal cytosolic and nuclear extracts and in freshly excised intact tissue strips. [3H]delta(5)-Androstene-3beta,17beta-diol bound to a protein(s) in the vaginal nuclear extract with high affinity (K(d)=3...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/j.steroids.2003.10.006
更新日期：2004-01-01 00:00:00
abstract：:The common disease asthma is characterized by the obstruction, inflammation and increased sensitivity of the airways. Glucocorticoids (GCs) are one of the most potent anti-inflammatory agents available for treating allergic disease. In this study, we report that the GC budesonide (BUD) can rapidly inhibit the histamin...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/j.steroids.2005.09.019
更新日期：2006-02-01 00:00:00
abstract：:Glucocorticoid receptor binding was compared in liver cytosol preparations from spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats using homologous displacement of [3H]dexamethasone. At 5 degrees C, there was no difference in receptor binding affinity or concentration between strains for dexamethasone, ...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/0039-128x(94)00011-z
更新日期：1995-01-01 00:00:00
abstract：:3 beta-Hydroxy-5-androsten-17-one is converted to 5-androstene-3, 17-dione by rat liver alcohol dehydrogenase (ADH). We have reported on the purity of the enzyme which is eluted with pyrazole as a single homogeneous protein using an AMP-agarose affinity column. Rat liver ADH can oxidize hydroxyl groups not only at 3 b...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/0039-128x(82)90079-4
更新日期：1982-11-01 00:00:00
abstract：:The biosynthesis of progesterone from [3H]pregnenolone was curvilinear over a 6 h time course in human placenta cytotrophoblasts and in human placenta choriocarcinoma cells (JEG-3 cells). Mass measurements determined independently by radioimmunoassay indicate that the progesterone synthesized by cytotrophoblasts (21.0...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/0039-128x(88)90043-8
更新日期：1988-05-01 00:00:00
abstract：:The palladium-catalyzed coupling of various 17-iodo-delta 16 steroids (17-iodo-androst-16-ene, 17-iodo-4-methyl-4-aza-androst-16-en-3-one, and 17-iodo-4-aza-androst-16-en-3-one) with dialkyl phosphites (dimethyl phosphite, diethyl phosphite, and diisopropyl phosphite) was examined in detail. The only successful condit...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/0039-128x(95)00121-6
更新日期：1995-12-01 00:00:00
abstract：:Brassinosteroids (BRs) are steroid hormones regulating various aspects of plant metabolism, including growth, development and stress responses. However, little is known about the mechanism of their impact on antioxidant systems and phospholipid turnover. Using tobacco plants overexpressing H+/Ca2+vacuolar Arabidopsis ...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/j.steroids.2019.108444
更新日期：2019-07-08 00:00:00
abstract：:A short and efficient synthesis of steroid synthons, di(tert-butyldimethylsilyl) ethers of 3,21-dihydroxy-24-nor-chol-5-en-23-al (8 and 10) and of ethyl 3,21-dihydroxy-25-homo-chola-5,23-dien-25-oate (9 and 11), having natural (20R) and unnatural (20S) configuration from 3β-(tert-butyldimethylsilyloxy)-14α,20ξ-card-5-...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/j.steroids.2011.01.010
更新日期：2011-04-01 00:00:00
abstract：:Progesterone and its polar metabolite(s) trigger the meiotic divisions in the amphibian oocyte through a non-genomic signaling system at the plasma membrane. Published site-directed mutagenesis studies of ouabain binding and progesterone-ouabain competition studies indicate that progesterone binds to a 23 amino acid e...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/j.steroids.2012.04.012
更新日期：2012-09-01 00:00:00
abstract：:While the neuroprotective benefits of estrogen and progesterone in critical illness are well established, the data regarding the effects of androgens are conflicting. Surgical repair of congenital heart disease is associated with significant morbidity and mortality, but there are scant data regarding the postoperative...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/j.steroids.2013.11.003
更新日期：2014-03-01 00:00:00
abstract：:Inhibition of 5alpha-reduction of testosterone by an anti-androgen TSAA-291 (16beta-ethyl-17beta-hydroxy-4-estren-3-one) was studied in rat ventral prostates and the metabolic conversion of 3H-TSAA-291 was examined both in vitro and in vivo. In the in vitro experiment using nuclear 5alpha-reductase of the prostate, 5a...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/0039-128x(81)90021-0
更新日期：1981-07-01 00:00:00
abstract：:Radioactive mestranol (ME) and/or ethynylestradiol (EE) were administered to women in Nigeria, Sri Lanka, and the USA, and the types and patterns of radioactive urinary conjugates examined by Sephadex LH-20 chromatography. There are no differences in the total excretion of urinary radioactivity over 3 days. Consistent...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/0039-128x(80)90001-x
更新日期：1980-09-01 00:00:00
abstract：:A virulent culture of Hymenoscyphus fraxineus, the causal agent of ash dieback, was investigated for its production of secondary metabolites in a 70 L batch fermentation. Chemical analysis of the mycelial extract by means of flash chromatography and preparative HPLC led to the isolation of a new ergostane-type steroid...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/j.steroids.2018.03.007
更新日期：2018-07-01 00:00:00
abstract：:Two-hydroxyestrone (2OHE-1) and 16alpha-hydroxyestrone (16OHE-1) are two estrogen metabolites that may play important roles in the development or promotion of breast cancer. Our study assessed the reliability of a newly developed kit procedure for measuring 2OHE-1. Although under certain conditions the assay would not...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/s0039-128x(00)00139-2
更新日期：2001-01-01 00:00:00
abstract：:Corticosteroids were used normally as anti-inflammatory drugs. However, in some area certain corticosteroids might be illegally used as growth promoting agent in feed, and as prohibited doping substances in game and sport for human or/and animal performance-enhancing. Synthesized structural similar corticosteroids wer...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/j.steroids.2020.108739
更新日期：2021-01-01 00:00:00
abstract：:The adverse effects of hypercortisolism on the human brain have been highlighted in previous studies of Cushing's disease (CD). However, the reversibility of brain damage after the resolution of hypercortisolism remains unclear. Thus, we studied the potential volumetric reversibility in biochemically remitted CD patie...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/j.steroids.2019.108457
更新日期：2019-11-01 00:00:00
