Abstract:
:A radioimmunoassay technique has been developed for the measurement of cortisol in a single methylene chloride extract of human plasma without chromatography. The antiserum, obtained by immunizing rabbits with cortisol-3-carboxymethyl-oxime conjugated to bovine serum albumin, had a high affinity (KA = 1.8 X 10(9) 1/mole) and capacity (2.3 X 10(-6) moles/L undiluted serum) for cortisol. The minimum detectable amount determined at the lower 95% confidence limit of the buffer control tubes was 8.3 +/- 4.7 pg/tube and a log dose - logit response standard curve was linear between 20 pg and 20 ng/tube. The antiserum was highly specific for cortisol with only corticosterone, cortisone, 11-deoxycortisol and 21-deoxycortisol showing significant cross-reaction (12.4, 6.6, 3.8 and 3.7%, respectively). The cross-reaction for the other tested naturally occurring and synthetic steroids did not exceed 1%. Regression analysis of cortisol concentration estimates obtained on 20 samples before and after Sephadex LH-20 column chromatography gave a coefficient of correlation (r) of 0.995 and a regression coefficient (b) of 1.04. Recovery of cortisol added to plasma samples was quantitative. The intra-assay error was 8.5% and the inter-assay error averaged 5.7%. The method is simple requiring a single solvent extraction of plasma, therefore permitting large numbers of samples to be handled efficiently by a single technician.
journal_name
Steroidsjournal_title
Steroidsauthors
Dash RJ,England BG,Midgley AR Jr,Niswender GDdoi
10.1016/0039-128x(75)90057-4subject
Has Abstractpub_date
1975-11-01 00:00:00pages
647-61issue
5eissn
0039-128Xissn
1878-5867pii
0039-128X(75)90057-4journal_volume
26pub_type
杂志文章相关文献
STEROIDS文献大全abstract::Steroid hormone is extensively used for transmitting variety of biological signals in organisms. Natural steroid hormone is synthesized from cholesterol in adrenal cortex and in sexual gland in vertebrates. Appropriately dosed synthetic steroid hormones can be used for medication. Despite their positive effects as med...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2015.01.015
更新日期:2015-04-01 00:00:00
abstract::Ru(II) steroidal metal complexes were synthesized by the reaction of dichlorodicarbonyl ruthenium(II) [Ru(CO)2Cl2]n with Steroidal thiosemicarbazones. Coordination via the thionic sulfur and the azomethine nitrogen atom of the thiosemicarbazone to the Ru(II) metal. Steroidal thiosemicarbazone derivatives were obtained...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2017.05.001
更新日期:2017-08-01 00:00:00
abstract::Circulation levels of estrone sulfate (E1S) and dehydroepiandrosterone sulfate (DHAS) have been measured in plasma using a radioimmunoassay for estrone and dehydroepiandrosterone following extraction and hydrolysis of the sulfate. The mean +/- SE concentrations of E1S and DHAS in normal men were 458 +/- 25 pg/ml and 1...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(79)80017-3
更新日期:1979-11-01 00:00:00
abstract::The luteinizing hormone (LH) surge initiates a cascade of proteolytic events that control ovulation. One of the genes induced by LH is the progesterone receptor (PR). Because mice with a mutant PR gene (PRKO) fail to ovulate and are infertile, we have used them as a model in which to determine PR target genes that mig...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(00)00114-8
更新日期:2000-10-01 00:00:00
abstract::The LNCaP-Fast Growing Colony (FGC) human prostate cancer cell line proliferates in response to the addition of dihydrotestosterone (DHT) 10(-10)-10(-8) M in charcoal-stripped serum-supplemented media. LNCaP-FGC cells will not attach or proliferate in serum-free conditions. LNCaP-FGC stock cultures were maintained in ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(93)90084-z
更新日期:1993-09-01 00:00:00
abstract::Mouse models of cystic fibrosis (CF) display increased sulfotransferase 1E1 (SULT1E1) activity in hepatocytes of cystic fibrosis transmembrane receptor (CFTR)-deficient animals. SULT1E1 is responsible for the sulfation and inactivation of beta-estradiol (E2) at physiological concentrations. IGF-1 message levels in CFT...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2008.09.002
更新日期:2009-01-01 00:00:00
abstract::We have previously demonstrated that 1α,25 dihydroxy-vitamin D(3) (1α,25(OH)(2)D(3)) has antiproliferative effects on the growth of endothelial cells transformed by the viral G protein-coupled receptor associated to Kaposi sarcoma (vGPCR). In this work, we have investigated whether 1α,25(OH)(2)D(3) exerts its growth i...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2012.05.006
更新日期:2012-09-01 00:00:00
abstract::Homogeneous catalytic hydrazinocarbonylation of some steroid derivatives possessing iodo-alkenyl moiety (17-iodo-androst-16-ene 1, 17-iodo-3-methoxy-estra-1,3,5(10),16-tetraene 2, 17-iodo-4-aza-4-methyl-androst-16-en-3-one 3 and 17-iodo-6beta-hydroxy-3alpha,5alpha-cycloandrost-16-ene 4) were carried out in the presenc...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(02)00006-5
更新日期:2002-06-01 00:00:00
abstract::Glucocorticoids (GCs) and progesterone have been employed as immunosuppressive agents during pregnancy for many years. Intracellular acidification by GCs is due to a rapid non-genomic inhibition of membrane Na(+)/H(+)-exchange 1 (NHE1) activity and is followed by immunosuppression of PHA-stimulated proliferation. NHE1...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2016.01.004
更新日期:2016-07-01 00:00:00
abstract::The reaction of 23-oxotigogenin acetate with TMSOTf in THF afforded the corresponding bisnorcholanic lactone in 60% yield. The analogous reactions carried out in dichloromethane or benzene gave the rearranged products--the isomeric spirostanic ketone (10-15%) and bisfuran (40-42%). Similar products were also obtained ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2009.02.010
更新日期:2009-08-01 00:00:00
abstract:BACKGROUND:Urinary steroid profiling by GC or GC-MS are established clinical tools to complement other biochemical tests in the diagnosis and investigation of a wide range of adrenocortical disorders, but normative data on adults using the more specific GC-MS are lacking. Our objective was to set up the reference inter...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2008.03.004
更新日期:2008-09-01 00:00:00
abstract::C17-20Lyase and 21-hydroxylase activities were measured during late gestation in the rhesus monkey (Macaca mulatta) fetal adrenal. Activities were assessed in 10,000 x g supernatants with 17-hydroxyprogesterone and NADPH as substrates. Although conversion of [14C]17-hydroxyprogesterone to [14C]androstenedione was note...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(81)90036-2
更新日期:1981-08-01 00:00:00
abstract::Parathyroid hormone-related protein (PTHrP) increases the growth and osteolytic potential of prostate cancer cells, making it important to control PTHrP expression in these cells. We show that 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) and its non-hypercalcemic analog, EB1089, decrease PTHrP mRNA and cellular protein leve...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2005.08.009
更新日期:2006-02-01 00:00:00
abstract::Glucocorticoids (GCs) are essential steroid hormones that regulate numerous metabolic and homeostatic functions in almost all physiological systems. Synthetic glucocorticoids are among the most commonly prescribed drugs for the treatment of various conditions including autoimmune, allergic and inflammatory diseases. G...
journal_title:Steroids
pub_type: 杂志文章,评审
doi:10.1016/j.steroids.2017.11.002
更新日期:2018-05-01 00:00:00
abstract::A highly active inhibitor of the binding of estrone and estradiol-17beta to rat alpha-fetoprotein is demonstrated for the first time in embryo, immature and adult rat sera as well as in fetal and adult human sera. The competitive character and the narrow specificity of this inhibition effect is shown. The major compou...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(77)80023-8
更新日期:1977-12-01 00:00:00
abstract::Bioconversion of the aromatase inhibitor formestane (4-hydroxyandrost-4-ene-3,17-dione) (1) by the fungus Rhizopus oryzae ATCC 11145 resulted in a new minor metabolite 3,5α-dihydroxyandrost-2-ene-4,17-dione (2) and the known 4β,5α-dihydroxyandrostane-4,17-dione (3) as the major product. The structural elucidation and ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2016.07.003
更新日期:2016-09-01 00:00:00
abstract::A new synthetic pathway towards secosteroidal macrocycles was described via a reaction of cycloaddition as the key step. The characteristic (1)H and (13)C NMR spectroscopic features of the synthesized compounds are reported. ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2013.12.002
更新日期:2014-02-01 00:00:00
abstract::Estradiol benzoate (EB) has been one of the most widely used estrogenic agents in animal husbandry, as a way of exogenously introducing the natural hormone estradiol-17β into the animal organism. Estradiol was previously employed to induce anabolic effects or reproductive improvements in cattle. However, the employmen...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2010.12.005
更新日期:2011-03-01 00:00:00
abstract::The hypothesis tested in the present work is that estrone non-genomically regulates aortic nitric oxide synthase (NOS) and cyclooxygenase (COX) activities in female rats, and that such regulation depends on ovarian function. We found that physiological concentrations of estrone (E(1)) (0.1-10nM) significantly increase...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2004.10.012
更新日期:2005-04-01 00:00:00
abstract::A series of final twelve propanoyloxy derivatives of 5β-cholan-24-oic acid (O-propanoyl derivatives of cholic acid) as potential drug absorption modifiers (skin penetration enhancers, intestinal absorption promoters) was generated by multistep synthesis. Structure confirmation of all generated compounds was accomplish...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2013.02.001
更新日期:2013-05-01 00:00:00
abstract::Hydroxycitric acid (HCA), a dietary-derived weight loss supplement, competitively inhibits ATP citrate lyase (ACLY). Tamoxifen (TAM) is the most frequently used therapy for estrogen receptor (ER)-positive breast cancer patients, but its application was restricted due to efficacy related issues. Lipid metabolic reprogr...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2020.108656
更新日期:2020-08-01 00:00:00
abstract::Two isomeric dimeric steroids, 3,3'-bis(methyl 3-hydroxyandrost-4-en-17-on-19-oate-3-yl), with symmetrical (alpha, alpha') and unsymmetrical structures (alpha,beta'), have been obtained by reduction of methyl androst-4-ene-3,17-dion-19-oate with zinc in aqueous acetic acid together with the major products, the isomeri...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(99)00108-7
更新日期:2000-04-01 00:00:00
abstract::Biotransformation of danazol (1) (17β-hydroxy-17α-pregna-2,4-dien-20-yno-[2,3-d]-isoxazole) with Cunninghamella blakesleeana yielded three new metabolites 2-4 and a known metabolite 5. These metabolites were identified as 14β,17β-dihydroxy-2-(hydroxymethyl)-17α-pregn-4-en-20-yn-3-one (2), 1α,17β-dihydroxy-17α-pregna-2...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2015.11.010
更新日期:2016-01-01 00:00:00
abstract::A heretofore unknown metabolite of vitamin D3 was isolated from the 1 alpha, 24, 25-trihydroxyvitamin D3 fraction of lipid extracts obtained from plasma of rats which were given intravenous or oral doses of 100 pmol/100 g of either 1 alpha-hydroxyvitamin D3 or 1 alpha, 23-dihydroxyvitamin D3. Doses of 25-250 pmoles of...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(80)90065-3
更新日期:1980-07-01 00:00:00
abstract::In addition to using radioimmunoassays for the determination of estrogens and other steroids, the possibility of using mass fragmentography for analysis was investigated. t-Butyldimethylsilyl chloride was selected as a reagent for derivatisation because it forms rather stable silylethers. In all the mass-spectra obrai...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(79)90013-8
更新日期:1979-04-01 00:00:00
abstract::Apoptosis is a highly organized mechanism that allows specific cells to die in a controlled manner. Apoptosis can be induced by a variety of agents in a great number of cell types, but cell shrinkage and discrete chromatin cleavage remain common components of apoptosis that is manifested by constitutive enzymes. Despi...
journal_title:Steroids
pub_type: 杂志文章,评审
doi:10.1016/s0039-128x(99)00034-3
更新日期:1999-09-01 00:00:00
abstract::Corticosteroids were used normally as anti-inflammatory drugs. However, in some area certain corticosteroids might be illegally used as growth promoting agent in feed, and as prohibited doping substances in game and sport for human or/and animal performance-enhancing. Synthesized structural similar corticosteroids wer...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2020.108739
更新日期:2021-01-01 00:00:00
abstract::15alpha-Hydroxydehydroisoandrosterone and 15alpha-hydroxypregnenolone were isolated from hydrolyzed extracts of human late pregnancy urine and identified by means of the isotope dilution technique. In two separate determinations the excretion rate of 15alpha-hydroxydehydroisoandrosterone was found to be 1.7 and 3.2 mi...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(78)80002-6
更新日期:1978-05-01 00:00:00
abstract::The four diastereomeric 3,16-diacetates 6, 9, 10 and 11 were prepared from gitoxin 1 by the routes shown in Schemes 1 and 2 and tested for inotropic activity in the isolated guinea-pig atrial preparation. In line with earlier findings in the digitoxigenin series, derivatives with a 3 alpha-acetoxy function, viz 9 and ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(85)90062-5
更新日期:1985-01-01 00:00:00
abstract::Catalytic cycloalumination of (3β,5α)-3-vinylcholestane and (3α,5α)-3-allylcholestane with Et3Al catalyzed by Cp2ZrCl2 was performed for the first time to give previously unknown aluminacyclopentanes in ∼90% yield; these products were converted in situ to carbo- and heterocyclic (5α)-cholestane derivatives. ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2016.01.015
更新日期:2016-04-01 00:00:00