Abstract:
:In addition to using radioimmunoassays for the determination of estrogens and other steroids, the possibility of using mass fragmentography for analysis was investigated. t-Butyldimethylsilyl chloride was selected as a reagent for derivatisation because it forms rather stable silylethers. In all the mass-spectra obrained from the steroid derivatives, one pronounced peak suitable for mass fragmentography was always present. Some of the spectra of the investigated estrogens, as well as testosterone, 3 alpha-hydroxy-5 alpha-androstan-17-one and 17 alpha-methyl-17 beta-hydroxy-5 alpha-androstan-3-one are discussed. The stability of various t-butyldimethylsilylethers and the rate of enolization of testosterone and progesterone in the presence of the silylation-agent under different conditions were established.
journal_name
Steroidsjournal_title
Steroidsauthors
Ballhorn L,Mueller WF,Korte Fdoi
10.1016/0039-128x(79)90013-8subject
Has Abstractpub_date
1979-04-01 00:00:00pages
379-88issue
4eissn
0039-128Xissn
1878-5867pii
0039-128X(79)90013-8journal_volume
33pub_type
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