Abstract:
:A cytochrome P450-deficient mutant of the pathogenic fungus, Candida albicans, which accumulates exclusively 14 alpha-methylsterols in place of the normal end product sterol, ergosterol, was examined for alterations in membrane fluidity by electron paramagnetic resonance. The results using four nitroxyl spin labels indicated that exponential phase cultures of the mutant strain, D10, had a uniformly more rigid membrane than similarly grown wild type. Since D10 shows a sterol spectrum similar to that of wild type cells treated with imidazole and triazole antifungal agents, many of the physiological effects reported as the result of azole application may be the result of alterations in membrane fluidity.
journal_name
Steroidsjournal_title
Steroidsauthors
Lees ND,Kleinhans FW,Broughton MC,Pennington DE,Ricker VA,Bard Mdoi
10.1016/0039-128x(89)90032-9subject
Has Abstractpub_date
1989-03-01 00:00:00pages
567-78issue
3-5eissn
0039-128Xissn
1878-5867pii
0039-128X(89)90032-9journal_volume
53pub_type
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