Abstract:
:The giant freshwater prawn, Macrobrachium rosenbergii, is important to many Asian countries due to its high economic value as an aquaculture product. With demand increasing, there is requirement for a better understanding of the biosynthetic components that regulate its growth and reproduction, including steroids, in order to help increase production. Vertebrate-type steroids and their receptors were identified in crustaceans and implicated in reproduction. In this study, we presented the sex steroids estradiol and progesterone by LC-MS/MS in female M. rosenbergii, and reveal steroidogenic-related genes by in silico analysis of de novo assembled transcriptomes. Comparative analysis with other species was performed to confirm their putative role, as well as tissue-specific and quantitative gene expression. We reveal 29 transcripts that encode for steroidogenic-related proteins, including steroidogenic enzymes, a nuclear steroid hormone receptors, and a steroidogenic factor. Moreover, we identified for the first time the presence of steroidogenic factor 1, StAR-related lipid transfer protein, estradiol receptor- and progesterone-like protein in M. rosenbergii. Those targeted for gene expression analysis (3 beta-hydroxysteroid dehydrogenase, 17 beta-hydroxysteroid dehydrogenase, estrogen sulfotransferase and progesterone receptor-like) showed widespread expression within many tissues, and at relatively high levels in the central nervous system (CNS) during ovarian maturation. In summary, we provide further evidence for the existence of steroidogenic pathways in crustaceans, which may be useful for advancing prawn aquaculture.
journal_name
Steroidsjournal_title
Steroidsauthors
Thongbuakaew T,Siangcham T,Suwansa-ard S,Elizur A,Cummins SF,Sobhon P,Sretarugsa Pdoi
10.1016/j.steroids.2016.01.006subject
Has Abstractpub_date
2016-03-01 00:00:00pages
149-60eissn
0039-128Xissn
1878-5867pii
S0039-128X(16)00007-6journal_volume
107pub_type
杂志文章相关文献
STEROIDS文献大全abstract::The hepatic metabolism of 3-oxoandrost-4-ene-17 beta-carboxylic acid (etienic acid), a probable acidic catabolite of deoxycorticosterone, was investigated using rats prepared with an external biliary fistula. Metabolic products were identified by GC-MS after hydrolysis with beta-glucuronidase and by proton nuclear mag...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(92)90052-b
更新日期:1992-07-01 00:00:00
abstract::Two isomeric dimeric steroids, 3,3'-bis(methyl 3-hydroxyandrost-4-en-17-on-19-oate-3-yl), with symmetrical (alpha, alpha') and unsymmetrical structures (alpha,beta'), have been obtained by reduction of methyl androst-4-ene-3,17-dion-19-oate with zinc in aqueous acetic acid together with the major products, the isomeri...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(99)00108-7
更新日期:2000-04-01 00:00:00
abstract::13beta-Ethyl-3-methoxy-17beta-ol-8,14-seco-1,3,5(10),8-gonatetraen-14-one (IIIa) was isolated and its participation in the well-known acidic cyclization process was established. ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(75)90015-x
更新日期:1975-05-01 00:00:00
abstract::Two variants of the Claisen rearrangement were evaluated for a stereoselective construction of a C-25 stereogenic center in cholestenoic acids based on 1,4-chirality transfer. Johnson orthoester Claisen rearrangement of (22R)- and (22S)-propargyl enol ethers proceeded in a highly stereoselective manner to give (25R)- ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2013.03.006
更新日期:2013-07-01 00:00:00
abstract::17-Hydroxyprogesterone is a well-known precursor of androstenedione in adrenal biosynthesis. This study using sheep adrenal incubations demonstrates that 11-deoxycortisol, the precursor of cortisol synthesis, also can be a precursor of androstenedione. Indeed, our data show that androstenedione synthesis is negatively...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(91)90112-9
更新日期:1991-01-01 00:00:00
abstract::The effect of sodium intake on the aldosterone metabolic clearance rate (MCR) was examined in 5 normal subjects. Measurements were made under conditions where dietary sodium ranged from 10 to 1500 meq/day. There were no consistent changes in MCR over these extremes of sodium intake, and the plasma level of aldosterone...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(81)90002-7
更新日期:1981-01-01 00:00:00
abstract:BACKGROUND:Once a septic condition is progressing, administration of steroids in the pro-inflammatory phase of septic shock ought to yield maximal effect on the subsequent, devastating inflammatory response. Recently, a retrospective study showed that early initiation of corticosteroid therapy improved survival in sept...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2012.06.001
更新日期:2012-09-01 00:00:00
abstract::The abilities of a variety of estrogens and antiestrogens to stimulate DNA synthesis in the prepuberal rat uterus were compared. One microgram of each compound was administered in vivo via a single intraperitoneal injection. DNA synthesis was assayed in vitro in isolated nuclei 24 h later. The relative mitogenicities ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(89)90096-2
更新日期:1989-08-01 00:00:00
abstract::A short and efficient synthesis of steroid synthons, di(tert-butyldimethylsilyl) ethers of 3,21-dihydroxy-24-nor-chol-5-en-23-al (8 and 10) and of ethyl 3,21-dihydroxy-25-homo-chola-5,23-dien-25-oate (9 and 11), having natural (20R) and unnatural (20S) configuration from 3β-(tert-butyldimethylsilyloxy)-14α,20ξ-card-5-...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2011.01.010
更新日期:2011-04-01 00:00:00
abstract::CGS 16949A inhibited the conversion of [4-14C]androstenedione (A) to [4-14C]estrone by human placental microsomes in a competitive manner (Ki = 1.6 nM). Aminoglutethimide, also a competitive inhibitor, had a Ki = 0.7 microM in this assay system. The Km for the aromatization of A was 0.11 microM. Using ovarian microsom...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(83)90068-5
更新日期:1987-07-01 00:00:00
abstract::Inhibition of concise enzymes in ergosterol biosynthesis is one of the most prominent strategies for antifungal chemotherapy. Nevertheless, the enzymes sterol C5-desaturase and sterol C22-desaturase, which introduce double bonds into the sterol core and side chain, have not been fully investigated yet for their potent...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2015.05.004
更新日期:2015-09-01 00:00:00
abstract::Sertoli cells isolated from 17 day old rats were maintained in culture and incubated with [14C]-progesterone for 20 h. The cells and media were extracted with ether/chloroform and the extracts chromatographed two-dimensionally on TLC and the radioactive metabolites visualized by autoradiography. Nine of the metabolite...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(80)80009-2
更新日期:1980-05-01 00:00:00
abstract::Mouse models of cystic fibrosis (CF) display increased sulfotransferase 1E1 (SULT1E1) activity in hepatocytes of cystic fibrosis transmembrane receptor (CFTR)-deficient animals. SULT1E1 is responsible for the sulfation and inactivation of beta-estradiol (E2) at physiological concentrations. IGF-1 message levels in CFT...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2008.09.002
更新日期:2009-01-01 00:00:00
abstract::The full assignments of the (1)H and (13)C NMR signals of steroids bearing the 16beta,23:23,26-diepoxy side chain are provided. Differentiation of the diasterotopic H-26 pair was achieved with the aid of NOESY experiments. The main substituent and steric effects associated with this moiety and their influence on the c...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2008.01.027
更新日期:2008-07-01 00:00:00
abstract::Estradiol benzoate (EB) has been one of the most widely used estrogenic agents in animal husbandry, as a way of exogenously introducing the natural hormone estradiol-17β into the animal organism. Estradiol was previously employed to induce anabolic effects or reproductive improvements in cattle. However, the employmen...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2010.12.005
更新日期:2011-03-01 00:00:00
abstract::Incubation of aldosterone with confluent layers of A6 (toad kidney) cells leads to its hydroxylation at the 6 beta-position. 6 beta-Hydroxyaldosterone is the major metabolite when the incubation is carried out at pH 6.8, whereas the product comprises 6 beta-hydroxy-17-isoaldosterone accompanied by some 6 beta-hydroxya...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(90)90084-o
更新日期:1990-11-01 00:00:00
abstract::The role of an estrogen-binding protein similar to a known mammalian estrogen receptor (ER) is described in the estradiol-dependent reproduction of the helminth parasite Taenia crassiceps. Previous results have shown that 17-β-estradiol induces a concentration-dependent increase in bud number of in vitro cultured cyst...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2011.05.003
更新日期:2011-09-01 00:00:00
abstract::To simultaneously measure some targeted endocrine disruptors and several forms of sex hormones in rat serum, an accurate analytical procedure was developed. First, a comparison between a polymeric-based solid-phase extraction (SPE) and a micro-extraction by packed sorbent was performed to choose the optimal method to ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2015.10.011
更新日期:2015-12-01 00:00:00
abstract::Prostaglandin E(2) (PGE(2)) is a major mediator in the pathophysiology, and pathogenesis of gynecological diseases associated with abnormal endometrial disease with proliferation and inflammation, such as endometriosis. In this study, we investigated the effect of dienogest, a selective progesterone receptor agonist, ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2010.08.010
更新日期:2011-01-01 00:00:00
abstract::Inhibition of 5alpha-reduction of testosterone by an anti-androgen TSAA-291 (16beta-ethyl-17beta-hydroxy-4-estren-3-one) was studied in rat ventral prostates and the metabolic conversion of 3H-TSAA-291 was examined both in vitro and in vivo. In the in vitro experiment using nuclear 5alpha-reductase of the prostate, 5a...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(81)90021-0
更新日期:1981-07-01 00:00:00
abstract::We describe a novel steroid, which we have named "Ionotropin". Its unique features are: [1] it has 23 carbon atoms and [2] it is a phosphocholine ester. There are no other known mammalian steroids with either structural feature. Ionotropin cross reacts with digoxin-specific antibodies and may be the long-sought, endog...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2018.03.001
更新日期:2018-08-01 00:00:00
abstract::Progesterone, estrone, estradiol-17 beta and corticosterone were quantified simultaneously for the first time in female American kestrel (Falco sparverius) plasma. A mean level for each hormone was determined for the laying and non-laying periods of the summer (April-September), and for February. Means were comparable...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(84)90012-6
更新日期:1984-04-01 00:00:00
abstract::It has now been more than 30 years since the laboratory verification of the localization of estrogen in certain animal tissues. Much has been learned since that time regarding the details of this process, including the presence of specific receptors for these hormones in target tissues, the mechanism of ligand binding...
journal_title:Steroids
pub_type: 杂志文章,评审
doi:10.1016/0039-128x(93)90069-y
更新日期:1993-06-01 00:00:00
abstract::Carbon-11-labeled casimiroin analogues were first designed and synthesized as new potential PET agents for imaging of quinone reductase (QR) 2 and aromatase expression in breast cancer. [(11)C]casimiroin (6-[(11)C]methoxy-9-methyl-[1,3]dioxolo[4,5-h]quinolin-8(9H)-one, [(11)C]11) and its carbon-11-labeled analogues 5,...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2010.06.004
更新日期:2010-12-01 00:00:00
abstract::The left adrenal of two female dogs were perfused with either 3 muCi [4-14C]cpd. S (2) and 45 muCi [1, 2-3H] 21-deoxycortisone (Dog I), or with 1 muCi [4-14C] 17-hydroxyprogesterone and 65 muCi [1,2-3H] 21-deoxycortisone (Dog II). In Dog I, 40% of the perfused 21-deoxycortisone was converted to cortisone and 23% of cp...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(77)90078-2
更新日期:1977-08-01 00:00:00
abstract::Enzyme immunoassays (EIA) are commonly utilized for the evaluation of androgens in biological fluids; however, careful consideration must be given to cross-reactivity with other endogenous sex-steroid hormones. Our purpose was to determine the validity of a commonly-utilized commercially-available dihydrotestosterone ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2013.08.013
更新日期:2013-12-11 00:00:00
abstract::The kinetics of 11 beta-hydroxysteroid dehydrogenase (11HSD) catalyzing the interconversion of cortisol (F) and cortisone (E) were compared in vitro following incubation of homogenates of human (N = 7) and baboon (N = 2) placenta. In both species, enzyme activity catalyzing the conversion of F to E was associated with...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(84)90004-7
更新日期:1984-09-01 00:00:00
abstract::Dioscorea panthaica is a traditional Chinese medicinal herb used in the treatment of various physiological conditions, including cardiovascular disease, gastropathy and hypertension. Steroidal saponins (SS) are the main active ingredients of this herb and have effects on myocardial ischemia and cancer. The phytochemic...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2014.12.023
更新日期:2015-03-01 00:00:00
abstract::During our studies of the hepatic androgen receptor in cynomolgus monkeys, tritiated mibolerone +/- a 200-fold excess of unlabeled mibolerone has been used to determine specific binding in cytosol. During time-course studies, high-capacity, unsaturable binding of [3H]mibolerone was noted after short-term incubations (...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(95)00116-8
更新日期:1995-11-01 00:00:00
abstract::This study aimed to develop a quantitative and in vivo magnetic resonance imaging (MRI) approach to investigate the muscle growth effects of anabolic steroids. A protocol of MRI acquisition on a standard clinical 1.5 T scanner and quantitative image analysis was established and employed to measure the individual muscl...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2007.12.011
更新日期:2008-04-01 00:00:00