Abstract：
:To simultaneously measure some targeted endocrine disruptors and several forms of sex hormones in rat serum, an accurate analytical procedure was developed. First, a comparison between a polymeric-based solid-phase extraction (SPE) and a micro-extraction by packed sorbent was performed to choose the optimal method to extract and concentrate the analytes: bisphenol A, atrazine, vinclozolin metabolite, testosterone, androstenedione, estrone, estradiol, estrone-sulfate and glucuronide and estradiol-sulfate and glucuronide. The analyses were then performed by high-performance liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) with electrospray ionisation in positive and negative modes. The protocol based on SPE was validated using the ICH/2005 guidelines. The validation demonstrated good performance in terms of linearity (R(2)>0.99), recovery (71-90%) and repeatability (relative standard deviation: 1-18%). The method was sensitive with LOQ comprised between 0.1 and 0.4 ng/ml for androgens and between 0.098 and 10.2 ng/ml for estrogens. The results obtained on the serum of rats exposed to the targeted endocrine disruptors showed the suitability of this analytical strategy.
journal_name
Steroidsjournal_title
Steroidsauthors
Tournier M,Pouech C,Quignot N,Lafay F,Wiest L,Lemazurier E,Cren-Olivé C,Vulliet Edoi
10.1016/j.steroids.2015.10.011subject
Has Abstractpub_date
2015-12-01 00:00:00pages
252-62eissn
0039-128Xissn
1878-5867pii
S0039-128X(15)00273-1journal_volume
104pub_type
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