Abstract:
:Incubation of aldosterone with confluent layers of A6 (toad kidney) cells leads to its hydroxylation at the 6 beta-position. 6 beta-Hydroxyaldosterone is the major metabolite when the incubation is carried out at pH 6.8, whereas the product comprises 6 beta-hydroxy-17-isoaldosterone accompanied by some 6 beta-hydroxyapoaldosterone at pH 7.4. All products were identified by high-field 1H nuclear magnetic resonance spectroscopy. Control experiments indicated that the side-chain isomerization to form the 17-iso and apo derivatives occurs after the cytochrome P 450-dependent synthesis of 6 beta-hydroxyaldosterone.
journal_name
Steroidsjournal_title
Steroidsauthors
Morris DJ,Latif SA,Conca TJ,Wei LT,Watlington CO,Kirk DN,Toms HC,Shackleton CHdoi
10.1016/0039-128x(90)90084-osubject
Has Abstractpub_date
1990-11-01 00:00:00pages
482-7issue
11eissn
0039-128Xissn
1878-5867pii
0039-128X(90)90084-Ojournal_volume
55pub_type
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