Abstract:
:Steroidogenesis begins with the metabolism of cholesterol to pregnenolone by the inner mitochondrial membrane cytochrome P450 side-chain cleavage (P450scc) enzyme. The rate of steroid formation, however, depends on the rate of cholesterol transport from intracellular stores to the inner mitochondrial membrane and loading of P450scc with cholesterol. In previous in vitro studies, we demonstrated that a key element in the regulation of cholesterol transport is the mitochondrial peripheral-type benzodiazepine receptor (PBR). We also showed that the polypeptide diazepam binding inhibitor (DBI), an endogenous PBR ligand, stimulates cholesterol transport and promotes loading of cholesterol to P450scc in vitro, and that its presence is vital for hCG-induced steroidogenesis by Leydig cells. Based on these data and the observations that i) the mitochondrial PBR binding and topography are regulated by hormones; ii) the 18-kDa PBR protein is functionally coupled to the mitochondrial contact site voltage-dependent anion channel protein; iii) the 18-kDa PBR protein is a channel for cholesterol, as shown by molecular modeling and in vitro reconstitution studies; iv) targeted disruption of the PBR gene in steroidogenic cells dramatically reduces the ability of the cells to transport cholesterol in the mitochondria and produce steroids; v) endocrine disruptors, with known anisteroidogenic effect, inhibit PBR ligand binding; and vi) in vivo reduction of adrenal PBR expression results in reduced circulating glucocorticoid levels, we conclude that PBR is an indispensable element of the steroidogenic machinery.
journal_name
Steroidsjournal_title
Steroidsauthors
Papadopoulos V,Amri H,Boujrad N,Cascio C,Culty M,Garnier M,Hardwick M,Li H,Vidic B,Brown AS,Reversa JL,Bernassau JM,Drieu Kdoi
10.1016/s0039-128x(96)00154-7subject
Has Abstractpub_date
1997-01-01 00:00:00pages
21-8issue
1eissn
0039-128Xissn
1878-5867pii
S0039-128X(96)00154-7journal_volume
62pub_type
杂志文章,评审相关文献
STEROIDS文献大全abstract::Brassinosteroid (BR) phytohormones play indispensable roles in plant growth and development. Brassinolide (BL) and 24-epibrassinolide (24-epiBL) are the most active ones among the BRs reported thus far. Unfortunately, the extremely low natural content and intricate synthesis process limit their popularization in agric...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2015.09.002
更新日期:2015-12-01 00:00:00
abstract::Sertoli cells isolated from 17 day old rats were maintained in culture and incubated with [14C]-progesterone for 20 h. The cells and media were extracted with ether/chloroform and the extracts chromatographed two-dimensionally on TLC and the radioactive metabolites visualized by autoradiography. Nine of the metabolite...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(80)80009-2
更新日期:1980-05-01 00:00:00
abstract::The effect of sodium intake on the aldosterone metabolic clearance rate (MCR) was examined in 5 normal subjects. Measurements were made under conditions where dietary sodium ranged from 10 to 1500 meq/day. There were no consistent changes in MCR over these extremes of sodium intake, and the plasma level of aldosterone...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(81)90002-7
更新日期:1981-01-01 00:00:00
abstract::The configurations at C-24 of 24-alkylsterols of six samples of Clerodendrum species (Verbenaceae) - the aerial parts of C. fragrans, C. inerme, C. infortunatum, C. scandens, and C. siphonanthus, and the seeds of C. infortunatum - were examined by NMR. All samples contained 24 beta-ethylsterols possessing a delta 25-b...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(89)90036-6
更新日期:1989-03-01 00:00:00
abstract::This study represents a continuing effort to find a new biomarker for the diagnosis and management of prostatic cancer. Polyclonal antibodies were prepared to a peptide (CAKP) representing amino acids 28 to 43 of the 5 alpha-reductase type 2 isozyme. Using immunoaffinity-purified antibodies, the sera of 62 patients we...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(96)00184-5
更新日期:1996-11-01 00:00:00
abstract::A radioimmunoassay technique has been developed for the measurement of cortisol in a single methylene chloride extract of human plasma without chromatography. The antiserum, obtained by immunizing rabbits with cortisol-3-carboxymethyl-oxime conjugated to bovine serum albumin, had a high affinity (KA = 1.8 X 10(9) 1/mo...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(75)90057-4
更新日期:1975-11-01 00:00:00
abstract::Prostaglandin E(2) (PGE(2)) is a major mediator in the pathophysiology, and pathogenesis of gynecological diseases associated with abnormal endometrial disease with proliferation and inflammation, such as endometriosis. In this study, we investigated the effect of dienogest, a selective progesterone receptor agonist, ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2010.08.010
更新日期:2011-01-01 00:00:00
abstract::A highly active inhibitor of the binding of estrone and estradiol-17beta to rat alpha-fetoprotein is demonstrated for the first time in embryo, immature and adult rat sera as well as in fetal and adult human sera. The competitive character and the narrow specificity of this inhibition effect is shown. The major compou...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(77)80023-8
更新日期:1977-12-01 00:00:00
abstract::Postmitochondrial supernatant (PMS) (1) has been prepared from the homogenate of rat seminal vesicles and the characteristics of the binding reaction of 5alpha-dihydrotestosterone (DHT) to the cytoplasmic androgen receptor have been studied using a charcoal adsorption procedure. At 0 degrees C apparent equilibrium of ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(77)80021-4
更新日期:1977-12-01 00:00:00
abstract::Inhibition of 5alpha-reduction of testosterone by an anti-androgen TSAA-291 (16beta-ethyl-17beta-hydroxy-4-estren-3-one) was studied in rat ventral prostates and the metabolic conversion of 3H-TSAA-291 was examined both in vitro and in vivo. In the in vitro experiment using nuclear 5alpha-reductase of the prostate, 5a...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(81)90021-0
更新日期:1981-07-01 00:00:00
abstract::It is well established that 1alpha,25-dihydroxyvitamin D(3) (1alpha,25(OH)(2)D(3)), the active metabolite of vitamin D, plays a role in regulating proliferation and differentiation of cells, in addition to its classic function in mineral homeostasis. Recent studies have also provided evidence for the involvement of 1a...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(00)00154-9
更新日期:2001-03-01 00:00:00
abstract::The reduction of 17-oxosteroids to 17β-hydroxysteroids is one of the important transformations for the preparation of many steroidal drugs and intermediates. The strain Zygowilliopsis sp. WY7905 was found to catalyze the reduction of C-17 carbonyl group of androst-4-ene-3,17-dione (AD) to give testosterone (TS) as the...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2016.11.002
更新日期:2017-02-01 00:00:00
abstract::Glucocorticoids (GCs) are essential steroid hormones that regulate numerous metabolic and homeostatic functions in almost all physiological systems. Synthetic glucocorticoids are among the most commonly prescribed drugs for the treatment of various conditions including autoimmune, allergic and inflammatory diseases. G...
journal_title:Steroids
pub_type: 杂志文章,评审
doi:10.1016/j.steroids.2017.11.002
更新日期:2018-05-01 00:00:00
abstract::We describe a novel steroid, which we have named "Ionotropin". Its unique features are: [1] it has 23 carbon atoms and [2] it is a phosphocholine ester. There are no other known mammalian steroids with either structural feature. Ionotropin cross reacts with digoxin-specific antibodies and may be the long-sought, endog...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2018.03.001
更新日期:2018-08-01 00:00:00
abstract::A short and efficient synthesis of steroid synthons, di(tert-butyldimethylsilyl) ethers of 3,21-dihydroxy-24-nor-chol-5-en-23-al (8 and 10) and of ethyl 3,21-dihydroxy-25-homo-chola-5,23-dien-25-oate (9 and 11), having natural (20R) and unnatural (20S) configuration from 3β-(tert-butyldimethylsilyloxy)-14α,20ξ-card-5-...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2011.01.010
更新日期:2011-04-01 00:00:00
abstract::An homogenate from cortical tissue of mare adrenals was incubated in the presence of tritiated pregnenolone. The (3H) androstenedione and the (3H) testosterone synthesized during the incubation were extracted, purified, and co-crystallized to constant specific activity in the presence of unlabeled carriers. The rate o...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(84)90033-3
更新日期:1984-02-01 00:00:00
abstract::Different phytoestrogens were tested as inhibitors of 17beta-hydroxysteroid dehydrogenase from the fungus Cochliobolus lunatus (17beta-HSDcl), a member of the short-chain dehydrogenase/reductase superfamily. Phytoestrogens inhibited the oxidation of 100microM 17beta-hydroxyestra-4-en-3-one and the reduction of 100micr...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2005.02.022
更新日期:2005-08-01 00:00:00
abstract::Gene therapy based on gene delivery is a promising strategy for the treatment of various human diseases such as cancer. Cationic lipids represent one of the important synthetic gene delivery systems. There is a great interest in imaging of gene therapy using the biomedical imaging technique positron emission tomograph...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2010.04.009
更新日期:2010-10-01 00:00:00
abstract::Novel C6-piperazine substituted purine nucleoside analogues (2-9) bearing a modified pyranose-like D ring of the 4-azasteroid moiety were efficiently synthesized through nucleophilic substitution at C6 position of the steroid-nucleoside precursors (1) with versatile piperazines. All newly-synthesized compounds were ev...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2013.12.004
更新日期:2014-04-01 00:00:00
abstract::The full assignments of the (1)H and (13)C NMR signals of steroids bearing the 16beta,23:23,26-diepoxy side chain are provided. Differentiation of the diasterotopic H-26 pair was achieved with the aid of NOESY experiments. The main substituent and steric effects associated with this moiety and their influence on the c...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2008.01.027
更新日期:2008-07-01 00:00:00
abstract::Preimplantation rabbit embryos were incubated with pregnenolone and dehydroisoandrosterone under conditions which gave formazan precipitation by the histochemical technique. The metabolic fate of the labeled steroids was assessed simultaneously. There was no concomitant transformation of pregnenolone to progesterone a...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(81)80012-8
更新日期:1981-02-01 00:00:00
abstract::Biotransformation of an orally active contraceptive drug, desogestrel (1), with Cunninghamella elegans yielded a new metabolite, 13β-ethyl-11-methylene-18,19-dinor-17α-pregn-4-en-20-yn-17β-ol-3,6-dione (2), along with five known metabolites, i.e., 13β-ethyl-11-methylene-18,19-dinor-17α-pregn-4-en-20-yn-3β,6β,17β-triol...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2020.108694
更新日期:2020-10-01 00:00:00
abstract::The LNCaP-Fast Growing Colony (FGC) human prostate cancer cell line proliferates in response to the addition of dihydrotestosterone (DHT) 10(-10)-10(-8) M in charcoal-stripped serum-supplemented media. LNCaP-FGC cells will not attach or proliferate in serum-free conditions. LNCaP-FGC stock cultures were maintained in ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(93)90084-z
更新日期:1993-09-01 00:00:00
abstract:BACKGROUND:Once a septic condition is progressing, administration of steroids in the pro-inflammatory phase of septic shock ought to yield maximal effect on the subsequent, devastating inflammatory response. Recently, a retrospective study showed that early initiation of corticosteroid therapy improved survival in sept...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2012.06.001
更新日期:2012-09-01 00:00:00
abstract::The characterization of new conjugated vitamin D metabolites in rat bile was performed using HPLC, liquid chromatography/tandem mass spectrometry combined derivatization, and GC-MS. After the administration of 24,25-dihydroxyvitamin D(3) to rats, 23, 25-dihydroxy-24-oxovitamin D(3) 23-glucuronide, 3-epi-24, 25-dihydro...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(00)00087-8
更新日期:2000-05-01 00:00:00
abstract::In this study, we assessed the rate of estradiol degradation via the 17 beta-hydroxysteroid dehydrogenase (HSD) enzyme in breast tumors from postmenopausal women. We initially studied the effects of time, level of enzyme activity, amount of tissue assayed, and substrate concentration on the linearity of conversion of ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(88)90020-7
更新日期:1988-03-01 00:00:00
abstract::Biologic sex and sex steroids are important factors in clinical and experimental stroke. This review evaluates key evidence that biological sex strongly alters mechanisms and outcomes from cerebral ischemia. The role of androgens in male stroke is understudied and important to pursue given that male sex is a well know...
journal_title:Steroids
pub_type: 杂志文章,评审
doi:10.1016/j.steroids.2009.10.014
更新日期:2010-11-01 00:00:00
abstract::Optimal assay conditions for cholesterol 7 alpha-hydroxylase activity in swine liver microsomes were determined. The enzyme activity is induced three-fold by feeding cholestyramine to the swine. This suggests that cholesterol 7 alpha-hydroxylase is likely to be the rate-limiting enzyme for biosynthesis of bile acids i...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(79)90008-4
更新日期:1979-03-01 00:00:00
abstract::An enzyme immunoassay for estrone in swine serum was established. For this, beta-galactosidase from E. coli was conjugated through estrone-17 (O-carboxymethyl)oxime using a mixed anhydride reaction. The percentage of immunoreactive estrone-17 (O-carboxymethyl)oxime-beta-galactosidase conjugate was estimated to be abou...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(82)90136-2
更新日期:1982-06-01 00:00:00
abstract::A variety of progestins are available for therapeutic use. It is convenient to classify them into those related in chemical structure to progesterone or testosterone. Progestins related to progesterone can be subdivided into pregnanes and 19-norpregnanes, whereas those related to testosterone can be subdivided into th...
journal_title:Steroids
pub_type: 杂志文章,评审
doi:10.1016/j.steroids.2003.08.003
更新日期:2003-11-01 00:00:00