Electrical responses to 1alpha,25(OH)2-Vitamin D3 and their physiological significance in osteoblasts.


:Osteoblasts are a main target for the steroid 1alpha,25(OH)2-Vitamin D3 (1,25D3), where a major outcome is the modulation of the bone remodeling process. 1,25D3 deficiency leads to clinical disorders such as osteomalacia and osteoporosis, characterized by a state of insufficiently calcified tissue and bone loss, respectively. In the osteoblast nucleus, 1,25D3 modulates gene transcription for the synthesis of bone matrix proteins via the Vitamin D receptor (VDR). At the plasma membrane level, 1,25D3 potentiates ion channel functions, activates signal transduction pathways, and increases cytoplasmic calcium concentrations. So far, no clear physiological significance has been attributed to membrane-initiated 1,25D3 actions in single cells. To investigate if (a) 1,25D3 is a modulatory agent of secretion in osteoblasts and (b) the classical VDR is involved in rapid electrical events in the cell membrane, we studied hormone effects on ion channel activities in relation to exocytosis in osteoblasts isolated from VDR knockout (KO) and wild-type (WT) mice. This paper is a retrospect of the electrophysiological studies done in our laboratory to date. We found that 1,25D3-promoted ion channel responses are coupled to secretion in calvarial osteoblasts, and develop only in the presence of a functional nuclear steroid VDR. This 1,25D3-regulated exocytosis in osteoblasts, which takes place within minutes of hormone application, seems to be the natural complement of genomic actions that evolve at a longer time scale. The absence of both 1,25D3 membrane and nuclear effects in VDR KO osteoblasts may explain bone abnormalities typically found in VDR KO mice.






Zanello LP,Norman AW




Has Abstract


2004-08-01 00:00:00
















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    abstract::In an endeavour to develop potent anti-tumor agents from diosgenin, a series of C-6 derived 1,2,3-triazolyl derivatives were designed and synthesized by employing Cu(I) catalyzed Huisgen 1,3-dipolar cycloaddition reaction of novel azides - (22R,25R)-6β-azidospirostan-3β,5α-diol and 6β-azido-7α-hydroxyspirosta-1,4-dien...


    pub_type: 杂志文章


    authors: Mironov ME,Oleshko OS,Pokrovskii MA,Rybalova TV,Pechurov VK,Pokrovskii AG,Cheresis SV,Mishinov SV,Stupak VV,Shults EE

    更新日期:2019-11-01 00:00:00

  • Cross-talk between cAMP and p53-generated signals in induction of differentiation and apoptosis in steroidogenic granulosa cells.

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    pub_type: 杂志文章,评审


    authors: Amsterdam A,Keren-Tal I,Aharoni D

    更新日期:1996-04-01 00:00:00

  • Unique effect of the pregnancy hormone estriol on antigen-induced production of specific antibodies in female BALB/c mice.

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    pub_type: 杂志文章


    authors: Ding J,Zhu BT

    更新日期:2008-03-01 00:00:00

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    pub_type: 杂志文章


    authors: Reach G,Nakane H,Nakane Y,Auzan C,Corvol P

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  • Synthesis and biological evaluation of novel C6-cyclo secondary amine substituted purine steroid-nucleosides analogues.

    abstract::Novel C6-cyclo secondary amine substituted purine steroid-nucleoside analogues (2-9) were efficiently synthesized through displacement of the C6 chloro on the purine ring of series 1 with versatile cyclic secondary amines, including pyrrolidines, piperidine, morpholine, and piperazines. All the newly-synthesized compo...


    pub_type: 杂志文章


    authors: Huang LH,Li Y,Xu HD,Zheng YF,Liu HM

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    pub_type: 杂志文章


    authors: Selman PJ,Wolfswinkel J,Mol JA

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  • The ecdysteroids from the tobacco hornworm during pupal-adult development five days after peak titer of molting hormone activity.

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    pub_type: 杂志文章


    authors: Kaplanis JN,Thompson MJ,Dutky SR,Robbins WE

    更新日期:1979-09-01 00:00:00

  • 15 beta-Hydroxysteroids (Part VII). Steroids of the human perinatal period: the synthesis of steroid markers and their radioactive tracers.

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    authors: Reeder AY,Joannou GE

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    pub_type: 杂志文章


    authors: Vanderstichele H,Eechaute W,Lacroix E,Leusen I

    更新日期:1989-06-01 00:00:00

  • Isolation of two new C30 sterols, (24E)-24-N-propylidenecholesterol and 24 epsilon-N-propylcholesterol, from a cultured marine Chrysophyte.

    abstract::Two new sterols, (24E)-24-n-propylidenecholesterol and 24 epsilon-n-propylcholesterol, were isolated from a cultured marine Chrysophyte. Since most of the steroids found in marine invertebrates are unchanged or modified sterols of algal or symbiotic origin, the discovery of these two unusual sterols in a unicellular a...


    pub_type: 杂志文章


    authors: Rohmer M,Kokke WC,Fenical W,Djerassi C

    更新日期:1980-02-01 00:00:00

  • Studies on the mechanism of action of 1 alpha,24-dihydroxyvitamin D3. III. The specific binding of 1 alpha,24-dihydroxyvitamin D3 to the receptor of chick parathyroid gland.

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    pub_type: 杂志文章


    authors: Ishizuka S,Bannai K,Naruchi T,Hashimoto Y

    更新日期:1982-01-01 00:00:00

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    pub_type: 杂志文章


    authors: Heckmann M,d'Uscio CH,de Laffolie J,Neuhaeuser C,Bödeker RH,Thul J,Schranz D,Frey BM

    更新日期:2014-03-01 00:00:00

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    pub_type: 杂志文章


    authors: Negi AS,Dwivedy I,Roy R,Ray S

    更新日期:1995-06-01 00:00:00

  • The distribution of 5 alpha-reductase and 3 alpha(beta)-hydroxysteroid dehydrogenase activities in the hyperplastic human prostate gland.

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    pub_type: 杂志文章


    authors: Habib FK,Beynon L,Chisholm GD,Busuttil A

    更新日期:1983-01-01 00:00:00

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    pub_type: 杂志文章


    authors: Davis RA,Showalter P,Kern F Jr

    更新日期:1975-10-01 00:00:00

  • (23S)- and (23R)-25-dehydro-1alpha-hydroxyvitamin D(3)-26,23-lactone function as antagonists of vitamin D receptor-mediated genomic actions of 1alpha,25-dihydroxyvitamin D(3).

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    pub_type: 杂志文章


    authors: Ishizuka S,Miura D,Ozono K,Saito M,Eguchi H,Chokki M,Norman AW

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  • Evidence that "embryonic estrogen" is a factor which controls the development of the mouse preimplantation embryo.

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    pub_type: 杂志文章


    authors: Gupta JS,Dey SK,Dickmann Z

    更新日期:1977-03-01 00:00:00

  • Biotransformation of contraceptive drug desogestrel with Cunninghamella elegans, and anti-inflammatory activity of its metabolites.

    abstract::Biotransformation of an orally active contraceptive drug, desogestrel (1), with Cunninghamella elegans yielded a new metabolite, 13β-ethyl-11-methylene-18,19-dinor-17α-pregn-4-en-20-yn-17β-ol-3,6-dione (2), along with five known metabolites, i.e., 13β-ethyl-11-methylene-18,19-dinor-17α-pregn-4-en-20-yn-3β,6β,17β-triol...


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    authors: Ibrahim I,Atia-Tul-Wahab,Khan N,Siddiqui M,Hassan Ajandouz E,Jabeen A,Mesmar J,Baydoun E,Iqbal Choudhary M

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    authors: Sholl SA,Wolf RC

    更新日期:1980-08-01 00:00:00

  • Synthesis and antiproliferative activity of 17-[1',2',3']-selenadiazolylpregnenolone compounds.

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    pub_type: 杂志文章


    authors: Thys-Jacobs S,Donovan D,Papadopoulos A,Sarrel P,Bilezikian JP

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  • Characterization of new conjugated metabolites in bile of rats administered 24,25-dihydroxyvitamin D(3) and 25-hydroxyvitamin D(3).

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    pub_type: 杂志文章


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    更新日期:2000-05-01 00:00:00

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    pub_type: 杂志文章


    authors: Chan AO,Taylor NF,Tiu SC,Shek CC

    更新日期:2008-09-01 00:00:00

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    pub_type: 杂志文章


    authors: Steinsapir J,Muldoon TG

    更新日期:1991-02-01 00:00:00

  • Synthesis of new steroidal quinolines with antitumor properties.

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    pub_type: 杂志文章


    authors: Yang YT,Du S,Wang S,Jia X,Wang X,Zhang X

    更新日期:2019-11-01 00:00:00

  • CAR and PXR: the xenobiotic-sensing receptors.

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    pub_type: 杂志文章,评审


    authors: Timsit YE,Negishi M

    更新日期:2007-03-01 00:00:00

  • Facile synthesis of carbon-11-labeled cholesterol-based cationic lipids as new potential PET probes for imaging of gene delivery in cancer.

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    pub_type: 杂志文章


    authors: Gao M,Wang M,Miller KD,Sledge GW,Hutchins GD,Zheng QH

    更新日期:2010-10-01 00:00:00

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    pub_type: 杂志文章


    authors: Sohár P,Bodor A,Schwartz R

    更新日期:1999-04-01 00:00:00

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    pub_type: 杂志文章


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    pub_type: 杂志文章,评审


    authors: Grossmann C,Ruhs S,Seiferth A,Gekle M

    更新日期:2010-08-01 00:00:00