Invalidation of a commercially available human 5α-dihydrotestosterone immunoassay.

abstract：:Two isoforms of the androgen receptor (AR-A and AR-B), differing by a lack of the first 187 amino acids in the NH2-terminal transactivation domain of AR-A, are expressed in connective tissue and bone. Transient transfections of normal human osteoblastic cells (HOB) and of genital skin fibroblasts defective in AR (GSF-...

journal_title：Steroids

authors： Liegibel UM,Sommer U,Boercsoek I,Hilscher U,Bierhaus A,Schweikert HU,Nawroth P,Kasperk C

更新日期：2003-12-01 00:00:00

abstract：:In this study, we investigated the binding characteristics of [3H]Delta(5)-androstene-3beta,17beta-diol to rabbit vaginal cytosolic and nuclear extracts and in freshly excised intact tissue strips. [3H]delta(5)-Androstene-3beta,17beta-diol bound to a protein(s) in the vaginal nuclear extract with high affinity (K(d)=3...

journal_title：Steroids

authors： Traish AM,Huang YH,Min K,Kim NN,Munarriz R,Goldstein I

更新日期：2004-01-01 00:00:00

abstract：:In an attempt to synthesize compounds with selective estrogen-receptor binding, fluoro- and amino-clomiphene were totally synthesized from benzyl chloride, and their estrogenic/antiestrogenic activity as well as that of some of their chemical intermediates was evaluated. The triazene prepared from the amino-clomiphene...

journal_title：Steroids

authors： Gazit A,Livshitz T,Shani J

更新日期：1986-07-01 00:00:00

abstract：:A convenient procedure for the synthesis of 5 beta-cholestane-3 alpha,7 alpha,12 alpha,25-tetrol via a modified homologation sequence of the intermediate 3 alpha,7 alpha,12 alpha-triformyloxy-24-oxo-25-diazo-25-homo-5 beta-cholane involving a homogeneous medium is described. This involves treating the intermediate alp...

journal_title：Steroids

authors： Dayal B,Bagan E,Speck J,Salen G

更新日期：1980-04-01 00:00:00

abstract：:The syntheses of 15beta-carboxyethylmercapto-5alpha-dihydrotestosterone, 15beta-carbosyethylmercapto-5alpha-androstane-3beta, 17beta-diol and 15beta-carboxyethylmercapto-5alpha-androstane-3alpha, 17beta-diol and the preparation of their bovine serum albumin (BSA) conjugates are described. These conjugates were employe...

journal_title：Steroids

authors： Rao PN,Khan AH,Moore PH Jr

更新日期：1977-02-01 00:00:00

abstract：:We developed a sensitive, reliable, and accurate LC/ESI-MS/MS method for measurement of 3β-sulfooxy-7β-N-acetylglucosaminyl-5-cholen-24-oic acid and its glycine and taurine amides in urine. This atypical C24 bile acid has been reported previously to be present in the urine of patients with Niemann-Pick Type C (NPC) di...

journal_title：Steroids

authors： Maekawa M,Misawa Y,Sotoura A,Yamaguchi H,Togawa M,Ohno K,Nittono H,Kakiyama G,Iida T,Hofmann AF,Goto J,Shimada M,Mano N

更新日期：2013-10-01 00:00:00

abstract：:For the purpose of describing the pathway by which estrones are synthesized in the rhesus monkey (Macaca mulatta) corpus luteum (CL), CL were obtained during the midluteal phase of the menstrual cycle and fragments incubated with equimolar amounts of [7-3H]pregnenolone plus [4-14C]progesterone. Metabolites including 3...

journal_title：Steroids

authors： Sholl SA,Wolf RC

更新日期：1980-08-01 00:00:00

abstract：:We have previously shown that the photoactive 4-azasteroid, [1,2 3H]N-4(benzylbenzoyl)-3-oxo-4-aza-4-methyl-5alpha-androst an-17beta-carboxamide is an effective probe of rat steroid 5alpha-reductase (isozyme-1) (5alphaR-1). In the current investigation, PEG-fractionated (6.5%) detergent-solubilized preparations contai...

journal_title：Steroids

authors： Bhattacharyya AK,Wang M,Rajagopalan K,Taylor MF,Hiipakka R,Liao S,Collins DC

更新日期：1999-03-01 00:00:00

abstract：BACKGROUND:Polycystic ovary syndrome (PCOS) is mainly defined by hyperandrogenemia, from ovarian and adrenal origin, and is characterized by insulin resistance (IR). Studies found that raising in vivo non-esterified fatty acid (NEFA) levels, which induces lipotoxicity, increases androgen levels and IR. The aim of this ...

journal_title：Steroids

authors： Bellanger S,Battista MC,Fink GD,Baillargeon JP

更新日期：2012-03-10 00:00:00

abstract：:Dehydroepiandrosterone (DHEA), an adrenal steroid, has a protective role against diabetes; however, its mechanisms of action are unknown. Here, we focus on the effect of DHEA on the activation of endothelial cells induced by a high concentration of glucose. Adhesion on U937 cells, expression of adhesion molecules, pro...

journal_title：Steroids

authors： Huerta-García E,Ventura-Gallegos JL,Victoriano ME,Montiél-Dávalos A,Tinoco-Jaramillo G,López-Marure R

更新日期：2012-02-01 00:00:00

abstract：:The configurations at C-24 of 24-alkylsterols of six samples of Clerodendrum species (Verbenaceae) - the aerial parts of C. fragrans, C. inerme, C. infortunatum, C. scandens, and C. siphonanthus, and the seeds of C. infortunatum - were examined by NMR. All samples contained 24 beta-ethylsterols possessing a delta 25-b...

journal_title：Steroids

authors： Akihisa T,Matsubara Y,Ghosh P,Thakur S,Tamura T,Matsumoto T

更新日期：1989-03-01 00:00:00

abstract：:The biotransformation of a series of delta 4-3-ketosteroids by the fungus Penicillium decumbens ATCC 10436 has been investigated. Conversion to the 5 alpha-dihydrosteroid was observed for several substrates of the androstene and pregnene series: the reaction is tolerant of non-polar substituents (Cl and CH3) at C-4 of...

journal_title：Steroids

authors： Holland HL,Dore S,Xu W,Brown FM

更新日期：1994-11-01 00:00:00

abstract：:Sex steroid-binding protein receptor was detected on membranes prepared from human premenopausal endometrium. The binding of sex steroid-binding protein to membranes was specific, saturable, and high affinity. Scatchard analysis showed the presence of two binding sites at different affinities. The addition of estradio...

journal_title：Steroids

authors： Fortunati N,Fissore F,Fazzari A,Berta L,Giudici M,Frairia R

更新日期：1991-06-01 00:00:00

abstract：:The purpose of this study was to investigate the influence of plasma steroid-binding proteins on androgen metabolism in intact leukocytes prepared from normal male and female blood samples. Leukocyte preparations were incubated for 24 h at 37 degrees C with either labeled or unlabeled testosterone (T), 5 alpha-dihydro...

journal_title：Steroids

authors： Déchaud H,Goujon R,Claustrat F,Boucherat M,Pugeat M

更新日期：1995-02-01 00:00:00

abstract：:Addition of soluble supernatant to testis microsomes results in 42% increase in steroid 17,20-lyase activity and a 65% increase in 17alpha-hydroxylase activity. This stimulatory activity could be partially purified by salt fractionation. The activating factor(s) was not removed by dialysis nor did it appear to be lipi...

journal_title：Steroids

authors： Betz G,Tsai P

更新日期：1978-10-01 00:00:00

abstract：:Testosterone (17 beta -hydroxy-4-androsten-3-one ; T) and dihydrotestosterone (17 beta -hydroxy-5 alpha -androstan-3-one ; DHT) concentrations were determined by radioimmunoassay in different fetal tissues taken from male guinea-pigs. Androgen concentrations were maximal in the components of the Wolffian duct (vas def...

journal_title：Steroids

authors： Rigaudière N

更新日期：1981-08-01 00:00:00

abstract：:The mass spectral elimination of water in epimeric 1,3-diols of vitamin D3 (colecalciferol) series has been investigated. It was found that the mass spectra of these steroisomers differ sharply in the relative intensities of the ions M-H2O (m/e 382) and a-H2O (m/e +/- 34), where ion a (formed via formal cleavage of th...

journal_title：Steroids

authors： Zaretskii ZV

更新日期：1979-05-01 00:00:00

abstract：:The hepatic metabolism of 3-oxoandrost-4-ene-17 beta-carboxylic acid (etienic acid), a probable acidic catabolite of deoxycorticosterone, was investigated using rats prepared with an external biliary fistula. Metabolic products were identified by GC-MS after hydrolysis with beta-glucuronidase and by proton nuclear mag...

journal_title：Steroids

authors： Radominska A,Little JM,Lester R,St Pyrek J

更新日期：1992-07-01 00:00:00

abstract：:The incorporation of the heterocycles into the steroid nucleus has been recognized as a useful strategy to develop new steroidal agents for disease treatment. Representative examples are abiraterone and galeterone, which are presently used in clinic for the treatment of advanced prostate cancers. Herein we have develo...

journal_title：Steroids

authors： Yang YT,Du S,Wang S,Jia X,Wang X,Zhang X

更新日期：2019-11-01 00:00:00

abstract：:Androstane brassinosteroid analogues with alpha-azido acid ester groups in position 17beta were synthesized from 2alpha,3alpha,17beta-trihydroxy-5alpha-androstan-6-one after the protection of the 2alpha,3alpha-diols upon treatment with the corresponding alpha-azido acid and the subsequent deprotection of the diol grou...

journal_title：Steroids

authors： Hnilickova J,Kohout L,Capdevila E,Esteve A,Vilaplana M,Molist M,Brosa C,Swaczynova-Oklestkova J,Slavikova B

更新日期：2010-12-01 00:00:00

abstract：:The functions of the group of proteins known as nuclear receptors will be understood fully only when their working three-dimensional structures are known. These ligand-activated transcription factors belong to the steroid-thyroid-retinoid receptor superfamily, which include the receptors for steroids, thyroid hormone,...

journal_title：Steroids

authors： Kumar R,Thompson EB

更新日期：1999-05-01 00:00:00

abstract：:This study was designed to describe the time-course changes of catabolic proteins following muscle atrophy induced by 10 days of dexamethasone (DEX). Rats underwent DEX treatment for 1, 3, 5, 7 and 10 days. Body weight (BW) and lean mass were obtained using a dual energy X-ray absorptiometry (DEXA) scan. Muscle ringer...

journal_title：Steroids

authors： Macedo AG,Krug AL,Souza LM,Martuscelli AM,Constantino PB,Zago AS,Rush JW,Santos CF,Amaral SL

更新日期：2016-03-01 00:00:00

abstract：:Steroidogenesis begins with the metabolism of cholesterol to pregnenolone by the inner mitochondrial membrane cytochrome P450 side-chain cleavage (P450scc) enzyme. The rate of steroid formation, however, depends on the rate of cholesterol transport from intracellular stores to the inner mitochondrial membrane and load...

journal_title：Steroids

authors： Papadopoulos V,Amri H,Boujrad N,Cascio C,Culty M,Garnier M,Hardwick M,Li H,Vidic B,Brown AS,Reversa JL,Bernassau JM,Drieu K

更新日期：1997-01-01 00:00:00

abstract：:(25S)-3-Oxocholesta-1,4-dien-26-oic acid (1) and a new (25S)-18-acetoxy-3-oxocholesta-1,4-dien-26-oic acid (2) were isolated from a soft coral Minabea sp. (cf. aldersladei) collected in North Sulawesi, Indonesia, together with two known cholic-acid-type compounds, 3-oxochol-1,4-dien-24-oic acid (3) and 3-oxochol-4-en-...

journal_title：Steroids

authors： Wang W,Lee JS,Nakazawa T,Ukai K,Mangindaan RE,Wewengkang DS,Rotinsulu H,Kobayashi H,Tsukamoto S,Namikoshi M

更新日期：2009-09-01 00:00:00

abstract：:Metandienone is one of the most frequently detected anabolic androgenic steroids in sports drug testing. Metandienone misuse is commonly detected by monitoring different metabolites excreted free or conjugated with glucuronic acid using gas chromatography mass spectrometry (GC-MS) and liquid chromatography tandem mass...

journal_title：Steroids

authors： Gómez C,Pozo OJ,Garrostas L,Segura J,Ventura R

更新日期：2013-12-11 00:00:00

abstract：:Parathyroid hormone-related protein (PTHrP) increases the growth and osteolytic potential of prostate cancer cells, making it important to control PTHrP expression in these cells. We show that 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) and its non-hypercalcemic analog, EB1089, decrease PTHrP mRNA and cellular protein leve...

journal_title：Steroids

authors： Sepulveda VA,Weigel NL,Falzon M

更新日期：2006-02-01 00:00:00

abstract：:A new synthetic pathway towards secosteroidal macrocycles was described via a reaction of cycloaddition as the key step. The characteristic (1)H and (13)C NMR spectroscopic features of the synthesized compounds are reported. ...

journal_title：Steroids

authors： Ibrahim-Ouali M,Hamze K

更新日期：2014-02-01 00:00:00

abstract：:The 11 beta-hydroxysteroid dehydrogenase type II enzyme (11 beta HSD2) converts cortisol to cortisone, allowing the non-selective mineralocorticoid receptor to bind aldosterone. When the activity of this enzyme is compromised, as occurs in licorice intoxication or in the congenital syndrome of apparent mineralocortico...

journal_title：Steroids

authors： Ferrari P,Obeyesekere VR,Li K,Andrews RK,Krozowski ZS

更新日期：1996-04-01 00:00:00

abstract：:A new technique that permits measurement of Androgen-Binding Protein (ABP) is validated by reproducibility, linearity and correlation studies. Using this apparatus allowing Scatchard plot analysis, it is also possible to measure association and dissociation rate constants. In addition, it is a very useful tool for a r...

journal_title：Steroids

authors： Barbey P,Fradin S,Carreau S,Drosdowsky MA

更新日期：1988-10-01 00:00:00

abstract：:Recent reports demonstrate neurotrophic properties of dehydroepiandrosterone sulfate (DHEAS) in men at rest, as well as profound neurotrophic responses to stress in both men and women. Little is known of neuroprotective-neurotrophic effects of DHEAS during stress exposure, either in men or women. This translational st...

journal_title：Steroids

authors： Taylor MK,Stone M,Laurent HK,Rauh MJ,Granger DA

更新日期：2014-09-01 00:00:00