A radioimmunoassay for 7 alpha-hydroxy dehydroepiandrosterone in human plasma.

Abstract:

:A radioimmunoassay for plasma 3 beta, 7 alpha-dihydroxy-5-androsten-17-one (7 alpha-hydroxy DHA) has been developed using anti-sera raised against 3 beta, 7 alpha-dihydroxy-5-androstene-17 beta-carboxyl-bovine serum albumin conjugate and [1, 2 (n) - 3H] 7 alpha-hydroxy DHA as the radioligand. Significant cross reactivity was found with 3 beta, 7 alpha-dihydroxy-5-pregnen-20-one (44%), 3 beta, 7 beta-dihydroxy-5-androsten-17-one (6%), 3 beta, 6 beta-dihydroxy-4-androsten-17-one (2.5%), 3 beta-hydroxy-5-androsten-17-one (DHA, 2%), 3 beta, 7 beta-dihydroxy-5-pregnen-20-one (2%) and 7 alpha-hydroxy-4-androstene-3, 20-dione (1%). 7 alpha-Hydroxy DHA was extracted from plasma and separated from cross-reacting factors using alumina micro-columns. The separation of bound and free steroid was achieved using dextran-coated charcoal. The concentration of 7 alpha-hydroxy DHA in the plasma of breast cancer patients was significantly lower than the concentrations in the plasma of normal women, hospitalized women suffering from non-endocrine diseases and patients with benign breast disease. The decrease in the concentration of 7 alpha-hydroxy DHA in the plasma of pregnant women was not significant.

journal_name

Steroids

journal_title

Steroids

authors

Skinner SJ,Tobler CJ,Couch RA

doi

10.1016/0039-128x(77)90023-x

subject

Has Abstract

pub_date

1977-09-01 00:00:00

pages

315-30

issue

3

eissn

0039-128X

issn

1878-5867

pii

0039-128X(77)90023-X

journal_volume

30

pub_type

杂志文章

相关文献

STEROIDS文献大全
  • Isolation and structure of a cytotoxic epoxy sterol from the marine mollusc Planaxis sulcatus.

    abstract::A novel epoxy sterol isolated from the marine mollusc Planaxis sulcatus has been identified as 9 alpha,11 alpha-epoxycholest-7-ene-3 beta,5 alpha,6 beta-triol by spectrometric methods. ...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/0039-128x(88)90216-4

    authors: Alam M,Sanduja R,Weinheimer AJ

    更新日期:1988-07-01 00:00:00

  • Novel action of estrone on vascular tissue: regulation of NOS and COX activity.

    abstract::The hypothesis tested in the present work is that estrone non-genomically regulates aortic nitric oxide synthase (NOS) and cyclooxygenase (COX) activities in female rats, and that such regulation depends on ovarian function. We found that physiological concentrations of estrone (E(1)) (0.1-10nM) significantly increase...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/j.steroids.2004.10.012

    authors: Selles J,Polini N,Alvarez C,Massheimer V

    更新日期:2005-04-01 00:00:00

  • Time-course changes of catabolic proteins following muscle atrophy induced by dexamethasone.

    abstract::This study was designed to describe the time-course changes of catabolic proteins following muscle atrophy induced by 10 days of dexamethasone (DEX). Rats underwent DEX treatment for 1, 3, 5, 7 and 10 days. Body weight (BW) and lean mass were obtained using a dual energy X-ray absorptiometry (DEXA) scan. Muscle ringer...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/j.steroids.2015.12.016

    authors: Macedo AG,Krug AL,Souza LM,Martuscelli AM,Constantino PB,Zago AS,Rush JW,Santos CF,Amaral SL

    更新日期:2016-03-01 00:00:00

  • Synthesis of new steroid haptens for radioimmunoassay. Part III. 15beta-Carboxyethylmercaptosteroid-bovine serum albumin conjugates. Specific antisera for radioimmunoassay of 5alpha-dihydrotestosterone, 5alpha-androstane-3beta, 17beta-diol and 5alpha-andr

    abstract::The syntheses of 15beta-carboxyethylmercapto-5alpha-dihydrotestosterone, 15beta-carbosyethylmercapto-5alpha-androstane-3beta, 17beta-diol and 15beta-carboxyethylmercapto-5alpha-androstane-3alpha, 17beta-diol and the preparation of their bovine serum albumin (BSA) conjugates are described. These conjugates were employe...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/0039-128x(77)90037-x

    authors: Rao PN,Khan AH,Moore PH Jr

    更新日期:1977-02-01 00:00:00

  • Comparison of flavonoids and isoflavonoids to inhibit rat and human 11β-hydroxysteroid dehydrogenase 1 and 2.

    abstract::Many flavonoids and isoflavonoids have anti-diabetic effects in animal models. However, the mechanisms that are involved are generally unclear. Since 11β-hydroxysteroid dehydrogenases (HSD11Bs) play important roles in diabetes, we hypothesize that flavonoids and isoflavonoids may affect diabetes by targeting two isofo...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/j.steroids.2018.01.013

    authors: Zhu Q,Ge F,Dong Y,Sun W,Wang Z,Shan Y,Chen R,Sun J,Ge RS

    更新日期:2018-04-01 00:00:00

  • Immunochemical studies on the putative plasmalemmal receptor for 1,25-dihydroxyvitamin D3 II. Chick kidney and brain.

    abstract::Chick kidney and brain were analyzed for the subcellular distribution (if any) of a putative plasma membrane receptor for 1,25-dihydroxyvitamin D3 [1,25(OH)2D3]. Fractionation protocols were found to be based not only on differential centrifugation conditions, but also gentleness of resuspension procedures, and suffic...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/s0039-128x(99)00030-6

    authors: Jia Z,Nemere I

    更新日期:1999-08-01 00:00:00

  • Naturally occurring marine steroid 24-methylenecholestane-3β,5α,6β,19-tetraol functions as a novel neuroprotectant.

    abstract::Steroids have been shown to have multiple effects on the nervous system including neuroprotective activities, and they have the potential to be used for the treatment of neurodegenerative diseases. In this current study, we tested the hypothesis that the marine steroid 24-methylenecholestane-3β,5α,6β,19-tetraol (Tetro...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/j.steroids.2015.11.005

    authors: Leng T,Liu A,Wang Y,Chen X,Zhou S,Li Q,Zhu W,Zhou Y,Su X,Huang Y,Yin W,Qiu P,Hu H,Xiong ZG,Zhang J,Yan G

    更新日期:2016-01-01 00:00:00

  • Role of microsomal receptors in steroid hormone action.

    abstract::Ventral prostate was used as a system to study the nature and properties of microsomal androgen receptor. The endoplasmic reticulum from rat ventral prostate contains high-affinity, low-capacity binding sites for androgen that are intrinsic to this intracellular compartment. Microsomal androgen receptors are not due t...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/0039-128x(91)90126-g

    authors: Steinsapir J,Muldoon TG

    更新日期:1991-02-01 00:00:00

  • Synthesis of biologically active steroid derivatives by the utility of Lawesson's reagent.

    abstract::Steroid derivatives V, VI, VII and VIII reacted with Lawesson's reagent (LR) to produce spiro-oxazaphosphole-4',17-androstene derivative XI, diazaphospholoandrostane XIV and the thionated derivatives XVI and XVII, respectively. The structures of the new compounds were confirmed by analytical and spectroscopic evidence...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/j.steroids.2004.11.001

    authors: Mohamed NR,Elmegeed GA,Abd-ElMalek HA,Younis M

    更新日期:2005-03-01 00:00:00

  • Allopregnanolone promotes proliferation and differential gene expression in human glioblastoma cells.

    abstract::Allopregnanolone (3α-THP) is one of the main reduced progesterone (P4) metabolites that is recognized as a neuroprotective and myelinating agent. 3α-THP also induces proliferation of different neural cells. It has been shown that P4 favors the progression of glioblastomas (GBM), the most common and aggressive primary ...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/j.steroids.2017.01.004

    authors: Zamora-Sánchez CJ,Hansberg-Pastor V,Salido-Guadarrama I,Rodríguez-Dorantes M,Camacho-Arroyo I

    更新日期:2017-03-01 00:00:00

  • Insulin alters the effects of follicle stimulating hormone on aromatase in bovine granulosa cells in vitro.

    abstract::It is known that follicle-stimulating hormone (FSH) and insulin stimulate estradiol secretion from cultured non-luteinizing granulosa cells. The interaction between these hormones is less well understood. Granulosa cells from small (2-4 mm) bovine follicles were cultured in serum-free medium to determine if cytochrome...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/s0039-128x(00)00218-x

    authors: Bhatia B,Price CA

    更新日期:2001-06-01 00:00:00

  • Tibolone and its metabolites enhance tissue factor and PAI-1 expression in human endometrial stromal cells: Evidence of progestogenic effects.

    abstract::Tibolone is a highly effective postmenopausal hormone treatment that has its biological activity dependent on metabolism to 3alpha- and 3beta-OH tibolone, which bind solely to the estrogen receptor. Despite the high levels of estrogen receptor-binding metabolites in the circulation, the endometrium becomes atrophic, s...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/j.steroids.2005.04.010

    authors: Schatz F,Kuczynski E,Kloosterboer HJ,Buchwalder L,Tang C,Krikun G,Lockwood CJ

    更新日期:2005-11-01 00:00:00

  • Adrenal steroidogenesis in the guinea pig: effects of androgens.

    abstract::In humans, the onset of adrenache has been found to occur with the appearance of the zona reticularis, the inner zone of the adrenal cortex. Since an increase in the volume of adrenal cortex during maturation in the guinea pig has been associated with the growth of the zona reticularis, we were interested in investiga...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/0039-128x(92)90033-6

    authors: Bélanger B,Caron S,Boudou P,Fiet J,Bélanger A

    更新日期:1992-02-01 00:00:00

  • Heterocyclic steroids-part VIII: Studies on the total synthesis of racemic 2-phenyl-7-methyl-3-oxa-A-nor-14 beta-estra-1,5(10),6,8-tetraen-17 alpha-ol.

    abstract::2-Phenyl-6-methyl-4-oxo-4,5,6,7-tetrahydrobenzofuran (II) is converted into racemic 2-phenyl-7-methyl-3-oxa-A-nor-14 beta-estra-1,5(10),6,8-tetraen-17 alpha-ol (VIII). ...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/0039-128x(79)90023-0

    authors: Ramadas SR,Padmanabhan S

    更新日期:1979-02-01 00:00:00

  • 21-Hydroxylation of C-11-oxygenated and C-11-deoxysteroids by perfused dog adrenals.

    abstract::The left adrenal of two female dogs were perfused with either 3 muCi [4-14C]cpd. S (2) and 45 muCi [1, 2-3H] 21-deoxycortisone (Dog I), or with 1 muCi [4-14C] 17-hydroxyprogesterone and 65 muCi [1,2-3H] 21-deoxycortisone (Dog II). In Dog I, 40% of the perfused 21-deoxycortisone was converted to cortisone and 23% of cp...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/0039-128x(77)90078-2

    authors: Horn H,Halperin G,Weidenfeld J,Mordohovich D,Maschler I

    更新日期:1977-08-01 00:00:00

  • Synthesis of 13C-labeled steroid hormones.

    abstract::The synthesis of 13C-labeled steroid hormones is reviewed. Two general approaches are highlighted: partial synthesis in which part of the steroid nucleus is replaced with 13C-labeled synthons, and total synthesis. Examples from both approaches, leading to (3-(3)C)-, (4-(13)C)-, (3,4-(13)C2)-, and (1,2,3,4-(13)C4)- lab...

    journal_title:Steroids

    pub_type: 杂志文章,评审

    doi:10.1016/0039-128x(90)90012-z

    authors: Zomer G,Stavenuiter JF

    更新日期:1990-10-01 00:00:00

  • Ultrahigh-performance liquid chromatography-ion trap mass spectrometry characterization of the steroidal saponins of Dioscorea panthaica Prain et Burkill and its application for accelerating the isolation and structural elucidation of steroidal saponins.

    abstract::Dioscorea panthaica is a traditional Chinese medicinal herb used in the treatment of various physiological conditions, including cardiovascular disease, gastropathy and hypertension. Steroidal saponins (SS) are the main active ingredients of this herb and have effects on myocardial ischemia and cancer. The phytochemic...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/j.steroids.2014.12.023

    authors: Wang W,Zhao Y,Jing W,Zhang J,Xiao H,Zha Q,Liu A

    更新日期:2015-03-01 00:00:00

  • Estrogen modulation of K(+) channel activity in hypothalamic neurons involved in the control of the reproductive axis.

    abstract::Here we report on the progress we have made in elucidating the mechanisms through which estrogen alters synaptic responses in hypothalamic neurons. We examined the modulation by estrogen of the coupling of various receptor systems to inwardly rectifying and small conductance, Ca(2+)-activated K(+) (SK) channels. We us...

    journal_title:Steroids

    pub_type: 杂志文章,评审

    doi:10.1016/s0039-128x(01)00181-7

    authors: Kelly MJ,Rønnekleiv OK,Ibrahim N,Lagrange AH,Wagner EJ

    更新日期:2002-05-01 00:00:00

  • Biotransformation and molecular docking studies of aromatase inhibitors.

    abstract::Bioconversion of the aromatase inhibitor formestane (4-hydroxyandrost-4-ene-3,17-dione) (1) by the fungus Rhizopus oryzae ATCC 11145 resulted in a new minor metabolite 3,5α-dihydroxyandrost-2-ene-4,17-dione (2) and the known 4β,5α-dihydroxyandrostane-4,17-dione (3) as the major product. The structural elucidation and ...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/j.steroids.2016.07.003

    authors: Martin GD,Narvaez J,Bulmer R,Durrant MC

    更新日期:2016-09-01 00:00:00

  • In human T cells mifepristone antagonizes glucocorticoid non-genomic rapid responses in terms of Na(+)/H(+)-exchange 1 activity, but not ezrin/radixin/moesin phosphorylation.

    abstract::Glucocorticoids (GCs) and progesterone have been employed as immunosuppressive agents during pregnancy for many years. Intracellular acidification by GCs is due to a rapid non-genomic inhibition of membrane Na(+)/H(+)-exchange 1 (NHE1) activity and is followed by immunosuppression of PHA-stimulated proliferation. NHE1...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/j.steroids.2016.01.004

    authors: Chien EJ,Hsu CH,Chang VH,Lin EP,Kuo TP,Chien CH,Lin HY

    更新日期:2016-07-01 00:00:00

  • 5 alpha-reductase inhibition by finasteride (Proscar) in epithelium and stroma of human benign prostatic hyperplasia.

    abstract::Finasteride is a specific 5 alpha-reductase inhibitor that has been shown to reduce the size of human benign prostatic hyperplasia (BPH) by inhibiting the intraprostatic conversion of testosterone to 5 alpha-dihydrotestosterone. The aim of the present in vitro study was to describe in more detail the inhibitory effect...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/0039-128x(94)90016-7

    authors: Weisser H,Tunn S,Debus M,Krieg M

    更新日期:1994-11-01 00:00:00

  • Prolonged progesterone treatment of endometrial epithelial cells modifies the effect of estradiol on their sensitivity to oxytocin.

    abstract::Estradiol (E2), progesterone (P4), and oxytocin (OT) are important for the initiation of luteolysis in ruminants but the mechanisms involved are still poorly understood. The objective of this study was to determine if duration of exposure of bovine endometrial epithelial cells to P4 affected the response of the cells ...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/s0039-128x(03)00094-1

    authors: Kombé A,Sirois J,Goff AK

    更新日期:2003-09-01 00:00:00

  • Human mammary tumor cell proliferation: primary role of platelet-derived growth factor and possible synergism with human alpha-fetoprotein.

    abstract::Human mammary medullary carcinoma cells (passages 16 to 21) were cultured for 2 days to allow for attachment, followed by 6 days of culture in either fetal calf serum, human cord blood, human amniotic fluid, or growth factors in the presence or absence of purified human alpha-fetoprotein (AFP). When growth factors wer...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/0039-128x(91)90042-t

    authors: Leal JA,Gangrade BK,Kiser JL,May JV,Keel BA

    更新日期:1991-05-01 00:00:00

  • Plasma and urinary markers of oral testosterone undecanoate misuse.

    abstract::Orally administered testosterone undecanoate (TU), an anabolic, androgenic steroid, can potentially be abused by athletes. Indirect evidence for detecting oral TU intake could be deduced from the changes in steroid profile post-administration. Direct evidence could be obtained by detection of unchanged TU in plasma. T...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/s0039-128x(01)00128-3

    authors: Peng SH,Segura J,Farré M,González JC,de la Torre X

    更新日期:2002-01-01 00:00:00

  • Isolation of histidyl peptides of the steroid-binding site of human placental estradiol 17 beta-dehydrogenase.

    abstract::Human placental estradiol 17 beta-dehydrogenase (E.C. 1.1.1.62) was inactivated at pH 6.3 by 3-bromo [2'-14C] acetoxy-1,3,5(10) estratrien-17-one, a know substrate. The affinity-alkylated enzyme was then hydrolyzed by trypsin. Radioactive peptides were initially isolated by gel filtration and identified according to w...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/0039-128x(82)90084-8

    authors: Murdock GL,Warren JC

    更新日期:1982-02-01 00:00:00

  • Membrane estrogen receptor signaling impacts the reward circuitry of the female brain to influence motivated behaviors.

    abstract::Within the adult female, estrogen signaling is well-described as an integral component of the physiologically significant hypothalamic-pituitary-gonadal axis. In rodents, the timing of ovulation is intrinsically entwined with the display of sexual receptivity. For decades, the importance of estradiol activating intrac...

    journal_title:Steroids

    pub_type: 杂志文章,评审

    doi:10.1016/j.steroids.2017.11.013

    authors: Tonn Eisinger KR,Larson EB,Boulware MI,Thomas MJ,Mermelstein PG

    更新日期:2018-05-01 00:00:00

  • Simplified radioimmunoassay for aldosterone using antisera to aldosterone-gamma-lactone.

    abstract::A simplified, non-chromatographic method for aldosterone-gamma-lactone (2) radio-immunoassay is described, using a high titer aldosterone-gamma-lactone antibody. This method takes six working hours for completion; the blanks are below the sensitivity of the assay (0-10 pg), and the intra and interassay coefficient of ...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/0039-128x(76)90004-0

    authors: Antunes JR,Dale SL,Melby JC

    更新日期:1976-11-01 00:00:00

  • Evidence for a glucocorticoid receptor beta splice variant in the rat and its physiological regulation in liver.

    abstract::Glucocorticoids are important regulators of metabolism and immune function. Synthetic glucocorticoids are extensively used for immunosuppression/anti-inflammatory therapy. Since the glucocorticoid receptor (GR) is central to most hormone effects; its in vivo regulation will influence hormone/drug action. An alternativ...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/j.steroids.2012.11.014

    authors: DuBois DC,Sukumaran S,Jusko WJ,Almon RR

    更新日期:2013-02-01 00:00:00

  • Measurement of bile acid synthesis by 14CO2: the metabolism of propionyl CoA.

    abstract::The relationship between 14CO2 evolution from the catabolism of [26 or 2714C] cholesterol to bile acids was studied in rats with biliary fistulae. When equal quantities of [26 or 2714C] cholesterol and [414C] cholesterol were administered, there was a significant linear relationship between 14CO2 expiration in the bre...

    journal_title:Steroids

    pub_type: 杂志文章

    doi:10.1016/0039-128x(75)90062-8

    authors: Davis RA,Showalter P,Kern F Jr

    更新日期:1975-10-01 00:00:00

  • Molecular analysis of 11 beta-hydroxysteroid dehydrogenase and its role in the syndrome of apparent mineralocorticoid excess.

    abstract::The syndrome of apparent mineralocorticoid excess (AME) is an inherited form of hypertension in which 11 beta-hydroxysteroid dehydrogenase (11-HSD) is defective. This enzyme converts cortisol to its inactive metabolite, cortisone. The deficiency allows mineralocorticoid receptors to be occupied by cortisol, because th...

    journal_title:Steroids

    pub_type: 杂志文章,评审

    doi:10.1016/s0039-128x(96)00164-x

    authors: White PC,Mune T,Rogerson FM,Kayes KM,Agarwal AK

    更新日期:1997-01-01 00:00:00