Enzyme immunoassay for cortisol in serum using cortisol 21-amine.

abstract：:Addition of soluble supernatant to testis microsomes results in 42% increase in steroid 17,20-lyase activity and a 65% increase in 17alpha-hydroxylase activity. This stimulatory activity could be partially purified by salt fractionation. The activating factor(s) was not removed by dialysis nor did it appear to be lipi...

journal_title：Steroids

authors： Betz G,Tsai P

更新日期：1978-10-01 00:00:00

abstract：:These studies investigated whether treatment with carbenoxolone (CBX), an inhibitor of 11 beta-hydroxysteroid dehydrogenase (11 beta-HSD), resulted in an enhanced mineralocorticoid response to endogenous or infused cortisol. In conscious sodium replete sheep with a parotid fistula, infusion of CBX (40 mg/h for 10 days...

journal_title：Steroids

authors： Dodic M,May CN,Coghlan JP

更新日期：1998-02-01 00:00:00

abstract：:The properties of 5-ene-3 beta hydroxysteroid oxidoreductase (3 beta-HSD) from human placental homogenates were studied in vitro. The apparent Michaelis constants for 3 beta-HSD with the substrates pregnenolone (delta 5P) and dehydroepiandrosterone (DHA) were 170 nM and nM respectively. The optimal pH for both these s...

journal_title：Steroids

authors： Yoshida N

更新日期：1979-01-01 00:00:00

abstract：:Bioassays were conducted to determine various endocrinological properties of two spirolactone derivatives, 4',5'-dihydrospiro-[estr-4-ene-17,2'(3'H)-furan]-3-one (Compound I) and dispiro[cyclopropane-1,6'-estr-4'-ene-17',2"(3"h)-furan]-3'-one (Compound II). They proved to be very potent estrogen antagonists in immatur...

journal_title：Steroids

authors： Brooks JR,Steelman SL,Patanelli DJ

更新日期：1977-06-01 00:00:00

abstract：:Antiandrogens are widely used agents for the treatment of androgen dependent diseases as inhibitors of androgen receptors (AR) action. Although the precise mechanism of antiandrogen action is not yet elucidated, recent studies indicate the involvement of the structure of the ligand in relation with the nuclear co-repr...

journal_title：Steroids

authors： Cabeza M,Bratoeff E,Ramírez E,Heuze I,Recillas S,Berrios H,Cruz A,Cabrera O,Perez V

更新日期：2008-09-01 00:00:00

abstract：:Neutral steroids are difficult to analyse using desorption ionisation methods coupled with mass spectrometry (MS). However, steroids with an unhindered ketone group can readily be derivatised with the Girard P (GP) reagent to give GP hydrazones. Steroid GP hydrazones contain a quaternary nitrogen atom and are readily ...

journal_title：Steroids

authors： Khan MA,Wang Y,Heidelberger S,Alvelius G,Liu S,Sjövall J,Griffiths WJ

更新日期：2006-01-01 00:00:00

abstract：:An enzyme immunoassay for estrone in swine serum was established. For this, beta-galactosidase from E. coli was conjugated through estrone-17 (O-carboxymethyl)oxime using a mixed anhydride reaction. The percentage of immunoreactive estrone-17 (O-carboxymethyl)oxime-beta-galactosidase conjugate was estimated to be abou...

journal_title：Steroids

authors： Tamamura F,Nakao T,Tsunoda N,Kawata K

更新日期：1982-06-01 00:00:00

abstract：:There is considerable evidence that although estradiol may trigger the preovulatory surge of gonadotropins, progesterone is required for its full magnitude and duration and that glucocorticoids bring about selective follicle-stimulating hormone release. The luteinizing hormone-releasing hormone (LHRH) neuron does not ...

journal_title：Steroids

authors： Mahesh VB,Brann DW

更新日期：1998-05-01 00:00:00

abstract：INTRODUCTION:ERα36 is a recently cloned variant of estrogen receptor-alpha which has been proved to play an active role in a series of malignant diseases. METHOD:ERα36 expression was examined using immunohistochemical methods with sections from 126 resected NSCLC specimens. The immunoreactivity of ERα66 was also studi...

journal_title：Steroids

authors： Zhang S,Qiu C,Wang L,Liu Q,Du J

更新日期：2014-09-01 00:00:00

abstract：:Steroidal nucleoside analogs were synthesized starting from testosterone. By reduction of the oxime of 17 beta-hydroxy-androst-4-en-3-one (testosterone), a mixture of the two amino epimers of C-3 were obtained. The 3 alpha-amino-androst-4-en-17 beta-ol was crystallized in 73% yield and coupled with 5-amino-4,6-dichlor...

journal_title：Steroids

authors： Gelpi ME,Cadenas RA,Mosettig J,Zuazo BN

更新日期：2002-03-01 00:00:00

abstract：:In comparison with the glucocorticoids cortisol and corticosterone, the mineralocorticoid aldosterone is a minority hormone of the mammalian adrenal cortex, and its proper function is dependent upon protective physiological mechanisms. These include a particular site of aldosterone synthesis in zona glomerulosa cells ...

journal_title：Steroids

authors： Müller J

更新日期：1995-01-01 00:00:00

abstract：:Structural modifications of natural products with complex structures like steroids require great synthetic effort. A review of literature is presented on the chemistry of the steroidal hormone 17α-methyltestosterone that is approved by Food and Drug Administration (FDA) in the United States as an androgen for estrogen...

journal_title：Steroids

authors： El-Desoky el-SI,Reyad M,Afsah EM,Dawidar AA

更新日期：2016-01-01 00:00:00

abstract：:During the second half of the luteal phase, the human corpus luteum becomes responsive to regular luteinizing hormone (LH) pulses. These LH pulses stimulate progesterone secretion tonically, and during this tonic stimulation, additional LH-independent progesterone pulses occur, which are particularly pronounced in wom...

journal_title：Steroids

authors： Wuttke W,Theiling K,Hinney B,Pitzel L

更新日期：1998-05-01 00:00:00

abstract：:A new synthetic pathway towards secosteroidal macrocycles was described via a reaction of cycloaddition as the key step. The characteristic (1)H and (13)C NMR spectroscopic features of the synthesized compounds are reported. ...

journal_title：Steroids

authors： Ibrahim-Ouali M,Hamze K

更新日期：2014-02-01 00:00:00

abstract：:Radioactive mestranol (ME) and/or ethynylestradiol (EE) were administered to women in Nigeria, Sri Lanka, and the USA, and the types and patterns of radioactive urinary conjugates examined by Sephadex LH-20 chromatography. There are no differences in the total excretion of urinary radioactivity over 3 days. Consistent...

journal_title：Steroids

authors： Williams MC,Goldzieher JW

更新日期：1980-09-01 00:00:00

abstract：:During our studies of the hepatic androgen receptor in cynomolgus monkeys, tritiated mibolerone +/- a 200-fold excess of unlabeled mibolerone has been used to determine specific binding in cytosol. During time-course studies, high-capacity, unsaturable binding of [3H]mibolerone was noted after short-term incubations (...

journal_title：Steroids

authors： Koritnik DR,Marschke KB,Koshy A

更新日期：1995-11-01 00:00:00

abstract：:Homogenates from human myometrium with an added NADPH regenerating system and ATP were incubated with 4-(14)C progesterone. Six metabolites were identified: 5alpha-pregnane-3, 20-dione, 5beta-pregnane-3, 20-dione, 3alpha-hydroxy-4-pregnen-20-one, 3beta hydroxy-4-pregnen-20-one. 20alpha-hydroxy-4-pregnen-3-one and 20be...

journal_title：Steroids

authors： Junkermann H,Runnebaum B,Lisboa BP

更新日期：1977-07-01 00:00:00

abstract：:Human progesterone receptor (PR) contains a polyproline motif in the amino-terminal domain that interacts with the SH3 domain of Src and mediates rapid activation of c-Src and downstream MAPK (Erk-1/-2) independent of the transcriptional activity of PR. Forcedly target PR to different locations in the cell by use of m...

journal_title：Steroids

authors： Boonyaratanakornkit V,Bi Y,Rudd M,Edwards DP

更新日期：2008-10-01 00:00:00

abstract：:We report the synthesis of 10 novel steroids obtained from 3 beta, 15 beta-diacetoxy-17 alpha-hydroxy-5-pregnen-20-one (1c) as intermediates in the synthesis of 15 beta-acetoxy-20,20-ethylenedioxy-17 alpha-hydroxy-4-pregnen-3-one (6a) and its tritiated tracer 15 beta-acetoxy-20,20-ethylenedioxy-17 alpha-hydroxy-1,2,6,...

journal_title：Steroids

authors： Reeder AY,Joannou GE

更新日期：1997-02-01 00:00:00

abstract：:We investigated the modulatory effect of estriol (E(3)), an estrogen predominantly produced during human pregnancy, on the antigen-induced production of specific antibodies in female BALB/c mice, and its effect was compared with 17beta-estradiol (E(2)). Estriol (E(3)) had a very different effect than E(2) on the antig...

journal_title：Steroids

authors： Ding J,Zhu BT

更新日期：2008-03-01 00:00:00

abstract：:A number of testosterone analogs with a 13,14-secosteroidal fragment have been prepared from (13S)-13-iodo-6beta-methoxy-3alpha, 5-cyclo-13,14-seco-5alpha-androstan-14,17-dione. The key steps involved stereoselective deiodination of the starting compound with triphenylphosphine and selective protection of the 17-keto ...

journal_title：Steroids

authors： Khripach VA,Zhabinskii VN,Kuchto AI,Zhiburtovich YY,Fando GP,Lyakhov AS,Govorova AA,Groen MB,van der Louw J,de Groot A

更新日期：2004-07-01 00:00:00

abstract：:Orally administered testosterone undecanoate (TU), an anabolic, androgenic steroid, can potentially be abused by athletes. Indirect evidence for detecting oral TU intake could be deduced from the changes in steroid profile post-administration. Direct evidence could be obtained by detection of unchanged TU in plasma. T...

journal_title：Steroids

authors： Peng SH,Segura J,Farré M,González JC,de la Torre X

更新日期：2002-01-01 00:00:00

abstract：:Saturation analysis of the binding of [3H]dexamethasone [( 3H]DEX) to ammonium sulfate precipitates (ASPs) confirmed the presence of a limited-capacity, high-affinity binder in human adipose tissue cytosols. Various non-radioactive steroids competed with [3H]DEX for binding to the ASPs in the following sequence: dexam...

journal_title：Steroids

authors： Miller LK,Kral JG,Strain GW,Zumoff B

更新日期：1987-06-01 00:00:00

abstract：:The synthesis of the esters of norethisterone (17 alpha-ethynyl-17 beta-hydroxyestr-4-en-3-one) with three bile acids and of the cholesteryl carbonate of norethisterone are described. :This article reports the synthesis of the esters of norethisterone (17 alpha-ethynl-17beta-hydroxyestr-4-en-3-one) with 3 bile acids ...

journal_title：Steroids

authors： Herz JE,Sandoval J

更新日期：1983-03-01 00:00:00

abstract：:17 beta-Hydroxysteroid dehydrogenase (17 beta-HSD) controls the last step in the formation of all androgens and all estrogens. This crucial role of 17 beta-HSD is performed by at least five 17 beta-HSD isoenzymes having individual cell-specific expression, substrate specificity, regulation mechanisms, and reductive or...

journal_title：Steroids

authors： Labrie F,Luu-The V,Lin SX,Labrie C,Simard J,Breton R,Bélanger A

更新日期：1997-01-01 00:00:00

abstract：:Formestane (F, androst-4-en-4-ol-3,17-dione) is an irreversible aromatase inhibitor with the ability to suppress the estrogen production from anabolic steroids. Consequently, F is mentioned on the World Anti-Doping Agency (WADA) prohibited list and because studies have shown that F is produced endogenously in small am...

journal_title：Steroids

authors： Polet M,Van Renterghem P,Van Gansbeke W,Van Eenoo P

更新日期：2013-11-01 00:00:00

abstract：:The 11 beta-hydroxysteroid dehydrogenase type II enzyme (11 beta HSD2) converts cortisol to cortisone, allowing the non-selective mineralocorticoid receptor to bind aldosterone. When the activity of this enzyme is compromised, as occurs in licorice intoxication or in the congenital syndrome of apparent mineralocortico...

journal_title：Steroids

authors： Ferrari P,Obeyesekere VR,Li K,Andrews RK,Krozowski ZS

更新日期：1996-04-01 00:00:00

abstract：:We have previously demonstrated that 1α,25 dihydroxy-vitamin D(3) (1α,25(OH)(2)D(3)) has antiproliferative effects on the growth of endothelial cells transformed by the viral G protein-coupled receptor associated to Kaposi sarcoma (vGPCR). In this work, we have investigated whether 1α,25(OH)(2)D(3) exerts its growth i...

journal_title：Steroids

authors： Gonzalez-Pardo V,D'Elia N,Verstuyf A,Boland R,Russo de Boland A

更新日期：2012-09-01 00:00:00

abstract：:The preparation of 9alpha, 11xi-tritiated 17alpha-ethinyl estradiol, mestranol, estradiol-17beta, and norethindrone are described. Estrone-3-methyl ether was employed as starting material, and ethinylation with lithium acetylide-ethylene diamine resulted in 95% mestranol. Demethylation of mestranol with boron tribro...

journal_title：Steroids

authors： Rao PN

更新日期：1971-08-01 00:00:00

abstract：:Bioconversion of the aromatase inhibitor formestane (4-hydroxyandrost-4-ene-3,17-dione) (1) by the fungus Rhizopus oryzae ATCC 11145 resulted in a new minor metabolite 3,5α-dihydroxyandrost-2-ene-4,17-dione (2) and the known 4β,5α-dihydroxyandrostane-4,17-dione (3) as the major product. The structural elucidation and ...

journal_title：Steroids

authors： Martin GD,Narvaez J,Bulmer R,Durrant MC

更新日期：2016-09-01 00:00:00