Abstract:
:Neutral steroids are difficult to analyse using desorption ionisation methods coupled with mass spectrometry (MS). However, steroids with an unhindered ketone group can readily be derivatised with the Girard P (GP) reagent to give GP hydrazones. Steroid GP hydrazones contain a quaternary nitrogen atom and are readily desorbed in the matrix-assisted laser desorption/ionisation (MALDI) process, giving an improvement in sensitivity of two orders of magnitude. Steroids without a ketone group, but with a 3beta-hydroxy-Delta5 function, can be readily converted to 3-oxo-Delta4 steroids and subsequently derivatised to GP hydrazones for MALDI analysis. In addition to giving strong [M]+ ions upon MALDI, steroid GP hydrazones give informative post-source decay (PSD) spectra. By using the accurate mass of the precursor-ion measured by MALDI-MS, in combination with the structural information encoded in its PSD spectrum, steroid structures can readily be determined.
journal_name
Steroidsjournal_title
Steroidsauthors
Khan MA,Wang Y,Heidelberger S,Alvelius G,Liu S,Sjövall J,Griffiths WJdoi
10.1016/j.steroids.2005.08.002subject
Has Abstractpub_date
2006-01-01 00:00:00pages
42-53issue
1eissn
0039-128Xissn
1878-5867pii
S0039-128X(05)00190-Xjournal_volume
71pub_type
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