WITHDRAWN: Development of a sensitive liquid phase-radioimmunoassay of 7α-methyl-19-nortestosterone.

abstract：:Steroid hormone analysis is widely used in health- and stress-related research to get insights into various diseases and the adaption to stress. Hair analysis has been used as a tool for the long-term monitoring of these steroid hormones. In this study, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) metho...

journal_title：Steroids

authors： Voegel CD,Hofmann M,Kraemer T,Baumgartner MR,Binz TM

更新日期：2020-02-01 00:00:00

abstract：:Apoptosis is a highly organized mechanism that allows specific cells to die in a controlled manner. Apoptosis can be induced by a variety of agents in a great number of cell types, but cell shrinkage and discrete chromatin cleavage remain common components of apoptosis that is manifested by constitutive enzymes. Despi...

journal_title：Steroids

authors： Montague JW,Bortner CD,Hughes FM Jr,Cidlowski JA

更新日期：1999-09-01 00:00:00

abstract：:Due to their high profile biological activity, the steroids are among the most important secondary metabolites. A review of literature on the synthesis of pentacyclic steroids is presented. ...

journal_title：Steroids

authors： Ibrahim-Ouali M

更新日期：2008-09-01 00:00:00

abstract：:A highly active inhibitor of the binding of estrone and estradiol-17beta to rat alpha-fetoprotein is demonstrated for the first time in embryo, immature and adult rat sera as well as in fetal and adult human sera. The competitive character and the narrow specificity of this inhibition effect is shown. The major compou...

journal_title：Steroids

authors： Benassayag C,Vallette G,Delorme J,Savu L,Nunez EA,Jayle MF

更新日期：1977-12-01 00:00:00

abstract：:Human placental estradiol 17 beta-dehydrogenase (E.C. 1.1.1.62) was inactivated at pH 6.3 by 3-bromo [2'-14C] acetoxy-1,3,5(10) estratrien-17-one, a know substrate. The affinity-alkylated enzyme was then hydrolyzed by trypsin. Radioactive peptides were initially isolated by gel filtration and identified according to w...

journal_title：Steroids

authors： Murdock GL,Warren JC

更新日期：1982-02-01 00:00:00

abstract：:Glycyrrhizic acid (GZA) and glycyrrhetinic acid (GRA) can be determined rapidly and precisely by high-performance liquid chromatography (HPLC) in biological fluids and tissues from experimental animals and humans. From plasma and tissues, GZA and GRA are extracted by organic solvents and the extracts can directly be u...

journal_title：Steroids

authors： Krähenbühl S,Hasler F,Krapf R

更新日期：1994-02-01 00:00:00

abstract：:Contraceptive methods for breastfeeding women should be safe for the mother and infant and should not interfere with lactation. Progestin-only methods meet these conditions and can be used from the sixth week postpartum. Because all progestins are excreted in milk, those that are insufficiently active by the oral rout...

journal_title：Steroids

authors： Massai R,Díaz S,Jackanicz T,Croxatto HB

更新日期：2000-10-01 00:00:00

abstract：:From the aerial parts of Euphorbia sogdiana Popov, obtusifoliol (1) and two related steroids (2-3) have been isolated and characterized along with a known cycloartane derivative (4). The chemical structure of the obtusifoliol-related compounds, obtained by 1D and 2D NMR, and MS measurements, have been determined as: 3...

journal_title：Steroids

authors： Aghaei M,Yazdiniapour Z,Ghanadian M,Zolfaghari B,Lanzotti V,Mirsafaee V

更新日期：2016-11-01 00:00:00

abstract：:We describe a novel steroid, which we have named "Ionotropin". Its unique features are: [1] it has 23 carbon atoms and [2] it is a phosphocholine ester. There are no other known mammalian steroids with either structural feature. Ionotropin cross reacts with digoxin-specific antibodies and may be the long-sought, endog...

journal_title：Steroids

authors： Chasalow F,Pierce-Cohen L

更新日期：2018-08-01 00:00:00

abstract：:17 beta-Hydroxysteroid dehydrogenase (HSD) was studied in endometrium removed surgically from intact female monkeys in the luteal phase of the menstrual cycle. Also castrated monkeys (N=20) implanted with estradiol (E2) and progesterone (P) in silastic capsules were used to investigate the hormonal dependence of this ...

journal_title：Steroids

authors： Kreitmann O,Kreitmann-Gimbal B,Bayard F,Hodgen GD

更新日期：1979-01-01 00:00:00

abstract：:A first assay based on stable isotope dilution/gas chromatography-mass spectrometry (ID/GC-MS) has been developed for plasma 11-deoxycortisol (Reichstein's compound S), the leading hormonal marker of 11beta-hydroxylase deficiency. A suitable internal standard being unavailable, we synthesized dideuterated 11-deoxycort...

journal_title：Steroids

authors： Wudy SA,Hartmann M,Homoki J

更新日期：2002-09-01 00:00:00

abstract：:The compounds named in the title have been synthesized from the di-(ethylene ketal) of 21-hydroxy-3,20-dioxo-19-norpregn-5-ene-18, 11 beta-lactone and its 5(10)-ene isomer. Reduction of this mixture 1 with sodium aluminum bis-(methoxyethoxy)hydride furnished the 11 beta, 18, 21-triol 2a. Conversion to the 18,21-diacet...

journal_title：Steroids

authors： Harnik M,Kashman Y,Carmely S,Cojocaru M,Dale SL,Holbrook MM,Melby JC

更新日期：1986-01-01 00:00:00

abstract：:21-Hydroxypregnenolone and its metabolite 5-pregnene-3 beta, 20 alpha 21-triol have been measured in the sulfate fraction of neonatal urine. These two steroids are the major two 21-hydroxylated 5-pregnenes produced by neonates and are almost exclusively excreted as disulfates. The excretions of these steroids by norma...

journal_title：Steroids

authors： Shackleton CH,Homoki J,Taylor NF

更新日期：1987-04-01 00:00:00

abstract：:A series of clickable bile acid-nucleosides conjugates linked directly or via amino acid linker were synthesized, and characterized by spectroscopic techniques such as 1H NMR, 13C NMR, FT-IR, HRMS and HPLC. The synthesized compounds 6a-p were screened for their in vitro anticancer property against a panel of three can...

journal_title：Steroids

authors： Agarwal DS,Siva Krishna V,Sriram D,Yogeeswari P,Sakhuja R

更新日期：2018-11-01 00:00:00

abstract：:Novel androstanopyrazoles have been efficiently synthesized from steroidal β-ketoaldehydes with different arylhydrazine hydrochlorides both under acidic and basic conditions. Knorr-type transformations of 16-hydroxymethylene-dehydroepiandrosterone containing its 1,3-dicarbonyl moiety on ring D, proved to be regioselec...

journal_title：Steroids

authors： Baji Á,Kovács F,Mótyán G,Schneider G,Wölfling J,Sinka I,Zupkó I,Ocsovszki I,Frank É

更新日期：2017-10-01 00:00:00

abstract：:Tibolone is a highly effective postmenopausal hormone treatment that has its biological activity dependent on metabolism to 3alpha- and 3beta-OH tibolone, which bind solely to the estrogen receptor. Despite the high levels of estrogen receptor-binding metabolites in the circulation, the endometrium becomes atrophic, s...

journal_title：Steroids

authors： Schatz F,Kuczynski E,Kloosterboer HJ,Buchwalder L,Tang C,Krikun G,Lockwood CJ

更新日期：2005-11-01 00:00:00

abstract：:The therapeutic use of progesterone following traumatic brain injury has recently entered phase III clinical trials as a means of neuroprotection. Although it has been hypothesized that progesterone protects against calcium overload following excitotoxic shock, the exact mechanisms underlying the beneficial effects of...

journal_title：Steroids

authors： Luoma JI,Kelley BG,Mermelstein PG

更新日期：2011-08-01 00:00:00

abstract：:Postmitochondrial supernatant (PMS) (1) has been prepared from the homogenate of rat seminal vesicles and the characteristics of the binding reaction of 5alpha-dihydrotestosterone (DHT) to the cytoplasmic androgen receptor have been studied using a charcoal adsorption procedure. At 0 degrees C apparent equilibrium of ...

journal_title：Steroids

authors： Zakár T,Tóth M

更新日期：1977-12-01 00:00:00

abstract：:A radioimmunoassay for plasma 3 beta, 7 alpha-dihydroxy-5-androsten-17-one (7 alpha-hydroxy DHA) has been developed using anti-sera raised against 3 beta, 7 alpha-dihydroxy-5-androstene-17 beta-carboxyl-bovine serum albumin conjugate and [1, 2 (n) - 3H] 7 alpha-hydroxy DHA as the radioligand. Significant cross reactiv...

journal_title：Steroids

authors： Skinner SJ,Tobler CJ,Couch RA

更新日期：1977-09-01 00:00:00

abstract：:The synthesis of 16 alpha-3H androgens and estrogens is described. 1-(3H)-Acetic acid in the presence of zinc dust reacts with 16 alpha-bromo-17-ketosteroids to produce 16 alpha-3H-17-ketosteroids. This chemical reaction was used to prepare 16 alpha-3H-dehydroepiandrosterone (I) and 16 alpha-3H-estrone acetate (XI) fr...

journal_title：Steroids

authors： Cantineau R,Kremers P,De Graeve J,Cornelis A,Laszlo P,Gielen JE,Lambotte R

更新日期：1981-02-01 00:00:00

abstract：:In this study, we assessed the rate of estradiol degradation via the 17 beta-hydroxysteroid dehydrogenase (HSD) enzyme in breast tumors from postmenopausal women. We initially studied the effects of time, level of enzyme activity, amount of tissue assayed, and substrate concentration on the linearity of conversion of ...

journal_title：Steroids

authors： Leszczynski D,Santner SJ,Feil PD,Santen RJ

更新日期：1988-03-01 00:00:00

abstract：:Readily available vanadyl acetylacetonate was found to oxidize the allylic sites of Δ(5) steroidal alcohols without protection of hydroxyl groups. Cholesterol, dehydroepiandrosterone, cholesterol benzoate, cholesterol acetate, pregnenolone, and 5-pregnen-3,20-diene were oxidized to 7-keto products using vanadyl acetyl...

journal_title：Steroids

authors： Grainger WS,Parish EJ

更新日期：2015-09-01 00:00:00

abstract：:Three new pregnane steroids, 2beta,3beta,5beta-trihydroxy-pregn-20-en-6-one (1), 3beta-hydroxy-5alpha-pregn-7,20-dien-6-one (2), and 3beta-acetoxy-5alpha-pregn-7,20-dien-6-one (3) were isolated from the twigs and leaves of Turraea pubescens, and were structurally elucidated on the basis of spectroscopic data and chemi...

journal_title：Steroids

authors： Wang XN,Fan CQ,Yue JM

更新日期：2006-08-01 00:00:00

abstract：BACKGROUND:A high-throughput, sensitive, specific, mass spectrometry-based method for quantitating estrone (E1), estradiol (E2), and testosterone (T) in postmenopausal human serum has been developed for clinical research. The method consumes 100μl human serum for each measurement (triplicates consume 300μl) and does no...

journal_title：Steroids

authors： Wooding KM,Hankin JA,Johnson CA,Chosich JD,Baek SW,Bradford AP,Murphy RC,Santoro N

更新日期：2015-04-01 00:00:00

abstract：:Testicular homogenates of tamoxifen-treated rats were incubated with labeled steroid precursors (progesterone, 17 alpha-hydroxyprogesterone, dehydroepiandrosterone, androstenedione or testosterone) in order to study the effect of tamoxifen on testicular steroidogenesis. The results indicate that a 9 day treatment with...

journal_title：Steroids

authors： Vanderstichele H,Eechaute W,Lacroix E,Leusen I

更新日期：1989-06-01 00:00:00

abstract：:Many flavonoids and isoflavonoids have anti-diabetic effects in animal models. However, the mechanisms that are involved are generally unclear. Since 11β-hydroxysteroid dehydrogenases (HSD11Bs) play important roles in diabetes, we hypothesize that flavonoids and isoflavonoids may affect diabetes by targeting two isofo...

journal_title：Steroids

authors： Zhu Q,Ge F,Dong Y,Sun W,Wang Z,Shan Y,Chen R,Sun J,Ge RS

更新日期：2018-04-01 00:00:00

abstract：:Brassinosteroids (BRs) are steroid hormones regulating various aspects of plant metabolism, including growth, development and stress responses. However, little is known about the mechanism of their impact on antioxidant systems and phospholipid turnover. Using tobacco plants overexpressing H+/Ca2+vacuolar Arabidopsis ...

journal_title：Steroids

authors： Kretynin SV,Kolesnikov YS,Derevyanchuk MV,Kalachova TA,Blume YB,Khripach VA,Kravets VS

更新日期：2019-07-08 00:00:00

abstract：:The relationship between 14CO2 evolution from the catabolism of [26 or 2714C] cholesterol to bile acids was studied in rats with biliary fistulae. When equal quantities of [26 or 2714C] cholesterol and [414C] cholesterol were administered, there was a significant linear relationship between 14CO2 expiration in the bre...

journal_title：Steroids

authors： Davis RA,Showalter P,Kern F Jr

更新日期：1975-10-01 00:00:00

abstract：BACKGROUND:Congenital adrenal hyperplasia (CAH) caused by 21-hydroxylase deficiency (21OHD) is an autosomal recessive disorder due to mutation in the CYP21A2 gene. OBJECTIVE:To elucidate the genetic basis of 21-hydroxylase-deficient CAH in Hong Kong Chinese patients. PATIENTS AND METHODS:Mutational analysis of the CY...

journal_title：Steroids

authors： Chan AO,But WM,Ng KL,Wong LM,Lam YY,Tiu SC,Lee KF,Lee CY,Loung PY,Berry IR,Brown R,Charlton R,Cheng CW,Ho YC,Tse WY,Shek CC

更新日期：2011-09-01 00:00:00

abstract：:The main purpose of this review article is threefold (a) to try to address the question "why are two adrenal glucocorticoids, cortisol and corticosterone, secreted by humans and other mammalian species?", (b) to outline a hypothesis that under certain physiological conditions, corticosterone has additional biochemical...

journal_title：Steroids

authors： Morris DJ

更新日期：2015-10-01 00:00:00