Abstract:
:The mechanism accounting for the development of castration-resistant prostate cancer (CRPC) remains unclear. Studies in CRPC tissues suggest that, after androgen deprivation therapy (ADT), the adrenal androgens may be an important source of testosterone (T) and 5-alpha dihydrotestosterone (DHT) in CRPC tissues. To clarify the role of adrenal androgens in the prostatic tissues (prostatic tissue adrenal androgens) during ADT, we developed a high sensitive and specific quantification method for the levels of androgens in prostatic tissue using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Human prostatic tissues were purified using mixed-mode reversed-phase, strong anion exchange Oasis cartridges (Oasis MAX). Analysis of steroids was performed using LC-MS/MS after picolinic acid derivatization. The validation tests showed that our method of quantitative analysis was precise and sensitive enough for the quantification of dehydroepiandrosterone (DHEA), androstenedione, androstenediol, T, and DHT in the prostatic tissue. The levels of adrenal androgens in prostate cancer tissues after ADT were similar to those in untreated PCa. Especially, DHEA was the most existing androgen precursor in PCa tissues after ADT. The levels of DHEA were high in PCa tissues, irrespective of ADT. We assumed that DHEA played a significant role in the synthesis of T and DHT in PCa tissues after ADT.
journal_name
Steroidsjournal_title
Steroidsauthors
Arai S,Miyashiro Y,Shibata Y,Tomaru Y,Kobayashi M,Honma S,Suzuki Kdoi
10.1016/j.steroids.2010.12.001subject
Has Abstractpub_date
2011-02-01 00:00:00pages
301-8issue
3eissn
0039-128Xissn
1878-5867pii
S0039-128X(10)00277-1journal_volume
76pub_type
杂志文章相关文献
STEROIDS文献大全abstract::This article describes the oxidation of cholesta-5,7-dien-3beta-yl acetate (4) with the urea-hydrogen peroxide adduct (UHP) using methyltrioxorhenium (MTO) as catalyst, under various conditions. Specifically, the effects of using different solvents (CHCl(3) and ethers) and additives (EtOH and pyridine) on the course o...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(02)00017-x
更新日期:2002-06-01 00:00:00
abstract::A novel epoxy sterol isolated from the marine mollusc Planaxis sulcatus has been identified as 9 alpha,11 alpha-epoxycholest-7-ene-3 beta,5 alpha,6 beta-triol by spectrometric methods. ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(88)90216-4
更新日期:1988-07-01 00:00:00
abstract::The abundant expression of progesterone receptors (PR) in human meningiomas is well established. It is unknown, however, how PR expression is regulated, especially since estrogen receptors (ER) are virtually absent in these tumors. At the mRNA level, ER splice variants occur in meningioma but these appear not to be in...
journal_title:Steroids
pub_type: 杂志文章,评审
doi:10.1016/s0039-128x(00)00193-8
更新日期:2000-10-01 00:00:00
abstract::15alpha-Hydroxydehydroisoandrosterone and 15alpha-hydroxypregnenolone were isolated from hydrolyzed extracts of human late pregnancy urine and identified by means of the isotope dilution technique. In two separate determinations the excretion rate of 15alpha-hydroxydehydroisoandrosterone was found to be 1.7 and 3.2 mi...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(78)80002-6
更新日期:1978-05-01 00:00:00
abstract::Smith-Lemli-Opitz syndrome (SLOS) is a malformation syndrome associated with 7-dehydrocholesterol (7DHC) 7-reductase deficiency. Although SLOS can be detected in an affected fetus before midpregnancy by measurement of 7DHC levels in amniotic fluid or chorionic villus cells, a noninvasive, more routine method is needed...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(99)00026-4
更新日期:1999-07-01 00:00:00
abstract::Three new polyhydroxysterols, named muriflasteroids A-C (1-3) were isolated from the South China Sea gorgonian Muriceopsis flavida, together with sixteen known analogs, cholest-3β,5α,6β-triol,3β-acetate (4), 5α-methoxycholest-3β,6β-diol (5), (22E)-cholest-22-en-3β,5α,6β-triol (6), cholest-3β,5α,6β-triol (7), (22E)-24-...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2012.10.003
更新日期:2013-01-01 00:00:00
abstract::We synthesized various analogues of 1alpha,25-(OH)(2)D(3)-26,23-lactone and examined the effects of them on HL-60 cell differentiation using the evaluation system of the genomic action of 1alpha,25-(OH)(2)D(3). We found that (23S)- and (23R)-25-dehydro-1alpha-OH-D(3)-26,23-lactone (TEI-9647 and TEI-9648) strongly boun...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(00)00146-x
更新日期:2001-03-01 00:00:00
abstract::The use of the synthetic progestin medroxyprogesterone acetate (MPA) for estrus prevention in the dog can result in overproduction of growth hormone, suppression of plasma glucocorticoid levels, and the induction of mammary tumors. Proligestone (PROL) was claimed to be devoid of these unwanted side effects. In the pre...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(95)00216-d
更新日期:1996-03-01 00:00:00
abstract::Three new pregnane steroids, 2beta,3beta,5beta-trihydroxy-pregn-20-en-6-one (1), 3beta-hydroxy-5alpha-pregn-7,20-dien-6-one (2), and 3beta-acetoxy-5alpha-pregn-7,20-dien-6-one (3) were isolated from the twigs and leaves of Turraea pubescens, and were structurally elucidated on the basis of spectroscopic data and chemi...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2006.04.010
更新日期:2006-08-01 00:00:00
abstract::Endochondral bone formation is a precise and highly ordered process whose exact regulatory framework is still being elucidated. Multiple regulatory pathways are known to be involved. In some cases, regulation impacts gene expression, resulting in changes in chondrocyte phenotypic expression and extracellular matrix sy...
journal_title:Steroids
pub_type: 杂志文章,评审
doi:10.1016/j.steroids.2017.12.003
更新日期:2019-02-01 00:00:00
abstract::Sedentary lifestyle and over-nutrition are the main causes of obesity and type 2 diabetes (T2D). However, the same causes are major triggers of hypogonadism. Many T2D patients show low testosterone levels while hypogonadal men seem to be prone to become diabetic. Testosterone plays a major role in the regulation of mu...
journal_title:Steroids
pub_type: 杂志文章,评审
doi:10.1016/j.steroids.2018.08.002
更新日期:2018-10-01 00:00:00
abstract::This study describes a new approach using stable isotope methodology in evaluating 11beta-HSD activities in vivo based on urinary excretion of cortisol, cortisone, and their A-ring reduced metabolites. The method involved the measurement of deuterium-labeled cortisol and its deuterium-labeled metabolites by GC/MS simu...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(99)00086-0
更新日期:2000-02-01 00:00:00
abstract::The condensation of O-substituted hydroxylamines with conjugated 3-oxo-4-en steroids results in a mixture of syn-anti configurations. Syn-anti ratios are influenced by sterical hindrance between the oximino substituents (e.g. O-benzyl) and the steroidal skeleton. Stereostructures and isomeric ratios were proved by det...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(98)00111-1
更新日期:1999-04-01 00:00:00
abstract::The ability of human adult adrenal to utilize progesterone (P4) for cortisol (F) synthesis in vitro has been compared with that of fetal adrenal tissue. Explant cultures were studied for 6 days using Ham's F10 medium supplemented with 10% fetal bovine serum (FBS), with or without added P4 as substrate. Short-term (4h)...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(86)90097-8
更新日期:1986-04-01 00:00:00
abstract::The Na-K-2Cl cotransporter (NKCC2) regulates sodium transport along the thick ascending limb of Henle's loop and is important in control of sodium balance, renal concentrating ability and renin release. To determine if there are sex differences in NKCC2 abundance and/or distribution, and to evaluate the contribution o...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2010.05.014
更新日期:2010-11-01 00:00:00
abstract::By the conventional methods of gas liquid chromatography (GLC) as well as mass spectrometry, 3beta,7alpha-dihydroxychol-5-en-24-oic acid (delta5-acid), a key intermediate of chenodeoxycholic acid biogenesis and its metabolic by-product, 3alpha,7alpha-dihydroxychol-4-en-24-oic acid (delta4-acid) have not yet been ident...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(78)90102-2
更新日期:1978-07-01 00:00:00
abstract::Bile acid derivatives, with and without C-3 sulfate groups, and having either the diazo- or halomethylketone moieties, have been synthesized in good yield and purity. The synthetic sequence, COOH leads to COC1 leads to COCHN2 leads to COCH2X, was used with deoxycholic and cholic acids, which requires carefully control...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(79)90046-1
更新日期:1979-08-01 00:00:00
abstract::The sterol mixture isolated from the marine alga Eutreptia viridis consists mainly of delta 5,7-dienes which account for 80% of the free sterols. Eighteen different sterols were detected, including a novel sterol with the rare delta 23-unsaturation, viz. 24-ethylcholesta-5,7,23Z-trien-3 beta-o1. ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(82)90018-6
更新日期:1982-10-01 00:00:00
abstract::Chick kidney and brain were analyzed for the subcellular distribution (if any) of a putative plasma membrane receptor for 1,25-dihydroxyvitamin D3 [1,25(OH)2D3]. Fractionation protocols were found to be based not only on differential centrifugation conditions, but also gentleness of resuspension procedures, and suffic...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(99)00030-6
更新日期:1999-08-01 00:00:00
abstract::Saturation analysis of the binding of [3H]dexamethasone [( 3H]DEX) to ammonium sulfate precipitates (ASPs) confirmed the presence of a limited-capacity, high-affinity binder in human adipose tissue cytosols. Various non-radioactive steroids competed with [3H]DEX for binding to the ASPs in the following sequence: dexam...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(87)90091-2
更新日期:1987-06-01 00:00:00
abstract::The crystal structures of chenodeoxycholic acid (CDCA), ursodeoxycholic acid (7beta isomer of CDCA) and their other two epimers (3beta,7alpha- and 3beta,7beta-isomers) have been resolved. The four isomers were recrystallized from p-xylene. CDCA crystal is hexagonal P6(5) while the crystals of the other three isomers a...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2007.03.007
更新日期:2007-06-01 00:00:00
abstract::We describe a convenient and stereoselective route to the synthesis of 27-hydroxycholesterol. Also its radiolabeled analog, 22, 23 di [(3)H]-27-hydroxycholesterol with high specific radioactivity (55 Ci/mmol) was synthesized by this method. Julia condensation of steroidal 22-sulfone with aldehyde, led to the addition ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(00)00099-4
更新日期:2000-07-01 00:00:00
abstract::Structural transformation of the steroidal lactone, oxandrolone (1), by suspended-cell cultures of the plant pathogen fungus Rhizopus stolonifer, resulted in the production of three new metabolites. These metabolites were identified as 11alpha-hydroxyoxandrolone (2), 6alpha-hydroxyoxandrolone (3) and 9alpha-hydroxyoxa...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2009.08.003
更新日期:2009-11-01 00:00:00
abstract::Plant lignans are currently being widely studied for their potential benefits for human health as their consumption has been correlated with lower risks for developing chronic diseases, such as breast cancer and coronary heart disease. However, studies of some classes of lignans, in particular the furofurans, are hamp...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2005.10.005
更新日期:2006-03-01 00:00:00
abstract::A highly active inhibitor of the binding of estrone and estradiol-17beta to rat alpha-fetoprotein is demonstrated for the first time in embryo, immature and adult rat sera as well as in fetal and adult human sera. The competitive character and the narrow specificity of this inhibition effect is shown. The major compou...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(77)80023-8
更新日期:1977-12-01 00:00:00
abstract::Ten new 9,11-secosteroids, subergorgols A-J (1-10), including two pairs of epimers (3/4, and 6/7), along with three known analogues (11-13) were isolated from the South China Sea gorgonian Subergorgia suberosa. The structures of these compounds were elucidated on the basis of extensive spectroscopic analyses and compa...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2013.05.009
更新日期:2013-09-01 00:00:00
abstract::Progesterone and its polar metabolite(s) trigger the meiotic divisions in the amphibian oocyte through a non-genomic signaling system at the plasma membrane. Published site-directed mutagenesis studies of ouabain binding and progesterone-ouabain competition studies indicate that progesterone binds to a 23 amino acid e...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2012.04.012
更新日期:2012-09-01 00:00:00
abstract::Determinations of oxygen solubility in serum were made utilizing various concentrations of corticosteroids in the serum. Within a certain concentration range dexamethasone and betamethasone caused an increase in oxygen solubility. None of the other steroids tested demonstrated this property. ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(76)90041-6
更新日期:1976-09-01 00:00:00
abstract::A radioimmunoassay technique has been developed for the measurement of cortisol in a single methylene chloride extract of human plasma without chromatography. The antiserum, obtained by immunizing rabbits with cortisol-3-carboxymethyl-oxime conjugated to bovine serum albumin, had a high affinity (KA = 1.8 X 10(9) 1/mo...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(75)90057-4
更新日期:1975-11-01 00:00:00
abstract::Structural modifications of natural products with complex structures like steroids require great synthetic effort. A review of literature is presented on the chemistry of the steroidal hormone 17α-methyltestosterone that is approved by Food and Drug Administration (FDA) in the United States as an androgen for estrogen...
journal_title:Steroids
pub_type: 杂志文章,评审
doi:10.1016/j.steroids.2015.11.004
更新日期:2016-01-01 00:00:00