Abstract:
:The Na-K-2Cl cotransporter (NKCC2) regulates sodium transport along the thick ascending limb of Henle's loop and is important in control of sodium balance, renal concentrating ability and renin release. To determine if there are sex differences in NKCC2 abundance and/or distribution, and to evaluate the contribution of ovarian hormones to any such differences, we performed semiquantitative immunoblotting and immunoperoxidase immunohistochemistry for NKCC2 in the kidney of Sprague Dawley male, female and ovariectomized (OVX) rats with and without 17-beta estradiol or progesterone supplementation. Intact females demonstrated greater NKCC2 protein in homogenates of whole kidney (334+/-29%), cortex (219+/-20%) and outer medulla (133+/-9%) compared to males. Ovarian hormone supplementation to OVX rats regulated NKCC2 in the outer medulla only, with NKCC2 protein abundance decreasing slightly in response to progesterone but increasing in response to 17-beta estradiol. Immunohistochemistry demonstrated prominent NKCC2 labeling in the apical membrane of thick ascending limb cells. Kidney section NKCC2 labeling confirmed regionalized regulation of NKCC2 by ovarian hormones. Localized regulation of NKCC2 by ovarian hormones may have importance in controlling sodium and water balance over the lifetime of women as the milieu of sex hormones varies.
journal_name
Steroidsjournal_title
Steroidsauthors
Musselman TM,Zhang Z,Masilamani SMdoi
10.1016/j.steroids.2010.05.014subject
Has Abstractpub_date
2010-11-01 00:00:00pages
760-5issue
11eissn
0039-128Xissn
1878-5867pii
S0039-128X(10)00130-3journal_volume
75pub_type
杂志文章相关文献
STEROIDS文献大全abstract::Ru(II) steroidal metal complexes were synthesized by the reaction of dichlorodicarbonyl ruthenium(II) [Ru(CO)2Cl2]n with Steroidal thiosemicarbazones. Coordination via the thionic sulfur and the azomethine nitrogen atom of the thiosemicarbazone to the Ru(II) metal. Steroidal thiosemicarbazone derivatives were obtained...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2017.05.001
更新日期:2017-08-01 00:00:00
abstract::The non-genomic action of steroid hormones regulates a wide variety of cellular responses including regulation of ion transport, cell proliferation, migration, death and differentiation. In order to achieve such plethora of effects steroid hormones utilize nearly all known signal transduction pathways. One of the key ...
journal_title:Steroids
pub_type: 杂志文章,评审
doi:10.1016/j.steroids.2008.01.001
更新日期:2008-10-01 00:00:00
abstract::The biotransformation of a series of delta 4-3-ketosteroids by the fungus Penicillium decumbens ATCC 10436 has been investigated. Conversion to the 5 alpha-dihydrosteroid was observed for several substrates of the androstene and pregnene series: the reaction is tolerant of non-polar substituents (Cl and CH3) at C-4 of...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(94)90020-5
更新日期:1994-11-01 00:00:00
abstract::Homogenates from human myometrium with an added NADPH regenerating system and ATP were incubated with 4-(14)C progesterone. Six metabolites were identified: 5alpha-pregnane-3, 20-dione, 5beta-pregnane-3, 20-dione, 3alpha-hydroxy-4-pregnen-20-one, 3beta hydroxy-4-pregnen-20-one. 20alpha-hydroxy-4-pregnen-3-one and 20be...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(77)90131-3
更新日期:1977-07-01 00:00:00
abstract::The reaction of 23-oxotigogenin acetate with TMSOTf in THF afforded the corresponding bisnorcholanic lactone in 60% yield. The analogous reactions carried out in dichloromethane or benzene gave the rearranged products--the isomeric spirostanic ketone (10-15%) and bisfuran (40-42%). Similar products were also obtained ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2009.02.010
更新日期:2009-08-01 00:00:00
abstract::3beta-Hydroxy sterols occurring at a concentration of at least 0.001% of the sterol mixtures of Pseudoplexaura porosa and Plexaura homomalla have been fractionated using a series of refined techniques and subsequently analyzed using combined gas chromatography-mass spectrometry (GC-MS) in the development of a procedur...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(76)90009-x
更新日期:1976-11-01 00:00:00
abstract::Progesterone, estrone, estradiol-17 beta and corticosterone were quantified simultaneously for the first time in female American kestrel (Falco sparverius) plasma. A mean level for each hormone was determined for the laying and non-laying periods of the summer (April-September), and for February. Means were comparable...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(84)90012-6
更新日期:1984-04-01 00:00:00
abstract::A series of chemiluminescent 17beta-estradiol probes were synthesized. Relative equilibrium dissociation constants (K(D)) for the interaction of an anti-E(2) Fab fragment for the probes in solution were evaluated using a single E(2)-analog biosensor surface on a BIAcore surface plasmon resonance instrument. The result...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(00)00091-x
更新日期:2000-06-01 00:00:00
abstract::An efficient procedure for the chemical synthesis of 3 beta-hydroxy-5 alpha-cholest-8-en-7-one and 3 beta-hydroxy-5 alpha-cholest-8-en-11-one is described. These ketosterols have been shown to have possible significant hypocholesterolemic effects when fed to normal rats at a level of 0.15% in a laboratory chow diet. T...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(86)90027-9
更新日期:1986-11-01 00:00:00
abstract::17-Hydroxyprogesterone is a well-known precursor of androstenedione in adrenal biosynthesis. This study using sheep adrenal incubations demonstrates that 11-deoxycortisol, the precursor of cortisol synthesis, also can be a precursor of androstenedione. Indeed, our data show that androstenedione synthesis is negatively...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(91)90112-9
更新日期:1991-01-01 00:00:00
abstract::Two isoforms of the androgen receptor (AR-A and AR-B), differing by a lack of the first 187 amino acids in the NH2-terminal transactivation domain of AR-A, are expressed in connective tissue and bone. Transient transfections of normal human osteoblastic cells (HOB) and of genital skin fibroblasts defective in AR (GSF-...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2003.08.016
更新日期:2003-12-01 00:00:00
abstract::Agave plants are popular for their myriad applications in traditional medicine attributed to their reported anti-inflammatory, immunomodulatory, cytotoxic and antifungal activities. The aim of this study was to examine the anti-inflammatory, immunomodulatory and ulceroprotective activity of Agave species in relation t...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2020.108648
更新日期:2020-08-01 00:00:00
abstract::Using 1 alpha,2 alpha-oxido-cholesta-5,7-diene-3 beta,25-diol (2) as a starting material, the provitamins of calcitriol with an additional 2 beta-chloro-, 2 beta-fluoro-, and 2 beta-methoxy-substituent (3,4,5) are obtained by transdiaxial opening of the oxirane ring with nucleophiles. An efficient irradiation process ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(98)00072-5
更新日期:1998-12-01 00:00:00
abstract::Key to the production of biologically active steroids is the enzyme 3 beta-hydroxysteroid dehydrogenase-isomerase. Some controversy has arisen concerning the subcellular distribution of this enzyme within steroidogenic cells. The distribution of 3 beta-hydroxysteroid dehydrogenase-isomerase was assessed in subcellular...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(91)90056-2
更新日期:1991-06-01 00:00:00
abstract::The hydrolysis of 108 esters of norethisterone (17 alpha-ethynyl-17 beta-hydroxyestr-4-en-3-one) was studied in vitro using a rabbit liver preparation. Introduction of a double or triple bond into a straight-chain ester did not inhibit hydrolysis but a marked reduction in hydrolysis was produced on replacement of a me...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(83)90110-1
更新日期:1983-03-01 00:00:00
abstract::Biotransformation of danazol (1) (17β-hydroxy-17α-pregna-2,4-dien-20-yno-[2,3-d]-isoxazole) with Cunninghamella blakesleeana yielded three new metabolites 2-4 and a known metabolite 5. These metabolites were identified as 14β,17β-dihydroxy-2-(hydroxymethyl)-17α-pregn-4-en-20-yn-3-one (2), 1α,17β-dihydroxy-17α-pregna-2...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2015.11.010
更新日期:2016-01-01 00:00:00
abstract::To reduce the variability in estradiol (E2) testing and to assure better patient care, standardization of E2 measurements has been recommended. This study aims to assess the accuracy and variability of E2 measurements performed by 11 routine immunological methods and 6 mass spectrometry methods using single donor seru...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2013.12.005
更新日期:2014-04-01 00:00:00
abstract::The relationship between 14CO2 evolution from the catabolism of [26 or 2714C] cholesterol to bile acids was studied in rats with biliary fistulae. When equal quantities of [26 or 2714C] cholesterol and [414C] cholesterol were administered, there was a significant linear relationship between 14CO2 expiration in the bre...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(75)90062-8
更新日期:1975-10-01 00:00:00
abstract::18,20-Epoxy-11 beta,17 alpha,20 beta,21-tetrahydroxypregn-4-en-3-one was synthesized by the application of hypoiodite reaction to the cortisol acetonide. The intermediary 18-iodo derivative was converted to the 11-oxo steroid by chromic acid prior to silver ion-assisted solvolysis. Removal of the protective group with...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(92)90095-q
更新日期:1992-09-01 00:00:00
abstract::The metabolism of a C26 bile alcohol (I, 24-nor-5beta-cho-lestane-3alpha, 7alpha,25-triol) was studied in the isolated perfused rabbit liver. The new bile alcohol and bile acid metabolites secreted into the bile were isolated and identified by a combination of TLC, GLC and GLC-MS. The following bile alcohols were foun...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(77)90099-x
更新日期:1977-10-01 00:00:00
abstract:BACKGROUND:Chronic administration of exogenous glucocorticoids is often required in experimental research. We compared the efficacy and reliability of three different methods of continuous glucocorticoid administration in mice. MATERIALS AND METHODS:Male CD1 Swiss White mice aged 7-9 weeks received corticosterone (CS)...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2008.11.009
更新日期:2009-02-01 00:00:00
abstract::The xenobiotic receptors CAR and PXR constitute two important members of the NR1I nuclear receptor family. They function as sensors of toxic byproducts derived from endogenous metabolism and of exogenous chemicals, in order to enhance their elimination. This unique function of CAR and PXR sets them apart from the ster...
journal_title:Steroids
pub_type: 杂志文章,评审
doi:10.1016/j.steroids.2006.12.006
更新日期:2007-03-01 00:00:00
abstract::The 3alpha,5alpha- and 3alpha,5beta-reduced derivatives of progesterone, deoxycorticosterone, dehydroepiandrosterone and testosterone enhance GABAergic neurotransmission and produce inhibitory neurobehavioral and anti-inflammatory effects. Despite substantial information on the progesterone derivative (3alpha,5alpha)-...
journal_title:Steroids
pub_type: 临床试验,杂志文章
doi:10.1016/j.steroids.2008.12.015
更新日期:2009-04-01 00:00:00
abstract::(24R and 24S)-5beta-cholestane-3alpha,7alpha,24,25-tetrols were prepared by osmium tetroxide oxidation of 5beta-cholest-24-ene-3alpha,7alpha-diol. The resulting diastereomeric tetrols were separated by thin-layer chromatography, their purity ascertained by melting point, gas-liquid chromatography and mass spectra and ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(78)90022-3
更新日期:1978-01-01 00:00:00
abstract::Neutral steroids are difficult to analyse using desorption ionisation methods coupled with mass spectrometry (MS). However, steroids with an unhindered ketone group can readily be derivatised with the Girard P (GP) reagent to give GP hydrazones. Steroid GP hydrazones contain a quaternary nitrogen atom and are readily ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2005.08.002
更新日期:2006-01-01 00:00:00
abstract::A phytochemical study of Physalis pubescens L. afforded twelve compounds, including six new withanolides (1, 4, and 6i-9), four new withanolide glucosides (2, 3, 5, and 6), and two known withanolides (10 and 11). Their structures were established via extensive spectroscopic analysis. The absolute configuration of 3 wa...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2016.09.002
更新日期:2016-11-01 00:00:00
abstract::The binding characteristics and quantitation of the recently reported fetal steroid binding protein (FSBP) cannot be determined on unpurified samples; an immunoassay was therefore desirable. The protein was purified to homogeneity in order to raise a highly specific polyclonal antibody. An enzyme-linked immunosorbent ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(85)90063-7
更新日期:1985-01-01 00:00:00
abstract:OBJECTIVE:To evaluate the role of physiologic levels of androgens and their precursors in the regulation of body composition, energy and substrate metabolism and aerobic capacity in healthy, cycling, premenopausal women. EXPERIMENTAL:We evaluated 30 young (27±1 year) premenopausal, non-obese (23±0.5 kg/m(2)), normally...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2011.06.001
更新日期:2011-11-01 00:00:00
abstract::The synthesis of seco-D and D-homo digitalis derivatives, from the carda-14,20(22)-dienolide 1, is described. Selective ozonolysis gave the seco-D 14-ketoaldehyde 2a. Modification of the two carbonyl groups and of the alpha, beta-unsaturated lactone ring of the seco-D 14-ketoaldehyde 2a allowed preparation of derivati...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(96)00117-1
更新日期:1996-10-01 00:00:00
abstract::The potent antiproliferative effect of progestins has been utilized in clinical regimens for treatment of endometrial proliferative disorders. The progestin infiltrated intrauterine device used as therapy for endometrial carcinoma as well as endometrial hyperplasia yields a hundred-fold increase of local progestin con...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2007.09.010
更新日期:2008-01-01 00:00:00