Abstract:
:A series of chemiluminescent 17beta-estradiol probes were synthesized. Relative equilibrium dissociation constants (K(D)) for the interaction of an anti-E(2) Fab fragment for the probes in solution were evaluated using a single E(2)-analog biosensor surface on a BIAcore surface plasmon resonance instrument. The results show the antibody fragment binds all chemiluminescent conjugates tested with high affinity showing only minor preferences for site of substitution (C6 versus C7), stereochemistry (alpha versus beta), or linker moiety.
journal_name
Steroidsjournal_title
Steroidsauthors
Adamczyk M,Chen YY,Gebler JC,Johnson DD,Mattingly PG,Moore JA,Reddy RE,Wu J,Yu Zdoi
10.1016/s0039-128x(00)00091-xsubject
Has Abstractpub_date
2000-06-01 00:00:00pages
295-303issue
6eissn
0039-128Xissn
1878-5867pii
S0039-128X(00)00091-Xjournal_volume
65pub_type
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