Evaluation of chemiluminescent estradiol conjugates by using a surface plasmon resonance detector.

Abstract:

:A series of chemiluminescent 17beta-estradiol probes were synthesized. Relative equilibrium dissociation constants (K(D)) for the interaction of an anti-E(2) Fab fragment for the probes in solution were evaluated using a single E(2)-analog biosensor surface on a BIAcore surface plasmon resonance instrument. The results show the antibody fragment binds all chemiluminescent conjugates tested with high affinity showing only minor preferences for site of substitution (C6 versus C7), stereochemistry (alpha versus beta), or linker moiety.

journal_name

Steroids

journal_title

Steroids

authors

Adamczyk M,Chen YY,Gebler JC,Johnson DD,Mattingly PG,Moore JA,Reddy RE,Wu J,Yu Z

doi

10.1016/s0039-128x(00)00091-x

subject

Has Abstract

pub_date

2000-06-01 00:00:00

pages

295-303

issue

6

eissn

0039-128X

issn

1878-5867

pii

S0039-128X(00)00091-X

journal_volume

65

pub_type

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