Cholecalciferol sulfate identification in human milk by HPLC.

abstract：:Enol aldehydes are one type of key degradation and metabolic intermediates from a group of corticosteroids containing the 1,3-dihydroxyacetone side chain on their D-rings, such as betamethasone, dexamethasone, beclomethasone, and related compounds. The formation of enol aldehydes from these corticosteroids is via acid...

journal_title：Steroids

authors： Chen B,Li M,Lin M,Tumambac G,Rustum A

更新日期：2009-01-01 00:00:00

abstract：:In addition to using radioimmunoassays for the determination of estrogens and other steroids, the possibility of using mass fragmentography for analysis was investigated. t-Butyldimethylsilyl chloride was selected as a reagent for derivatisation because it forms rather stable silylethers. In all the mass-spectra obrai...

journal_title：Steroids

authors： Ballhorn L,Mueller WF,Korte F

更新日期：1979-04-01 00:00:00

abstract：:The characterization of new conjugated vitamin D metabolites in rat bile was performed using HPLC, liquid chromatography/tandem mass spectrometry combined derivatization, and GC-MS. After the administration of 24,25-dihydroxyvitamin D(3) to rats, 23, 25-dihydroxy-24-oxovitamin D(3) 23-glucuronide, 3-epi-24, 25-dihydro...

journal_title：Steroids

authors： Higashi T,Miura K,Kikuchi R,Shimada K,Hiyamizu H,Ooi H,Iwabuchi Y,Hatakeyama S,Kubodera N

更新日期：2000-05-01 00:00:00

abstract：:In this paper, we studied the effect of heme availability on corticosterone and aldosterone synthesis in rat adrenal. We found that hemin stimulated corticosterone and aldosterone production in adrenal homogenates in a dose-dependent fashion. Hemin administration to rats also provoked an increase in both corticosteron...

journal_title：Steroids

authors： Martini CN,Vaena de Avalos SG,Romero DG,San Martín de Viale L,Vila Mde C

更新日期：1997-12-01 00:00:00

abstract：:In this paper, we examine corticosteroid 11 beta-oxidation and 11-reduction as properties of the microsomal 11 beta-hydroxysteroid dehydrogenase complex in vertebrate livers. No hepatic activity in the oxidative direction (11 beta -dehydrogenase) was found in the frog, toad, mud puppy, shark, and bird livers. In contr...

journal_title：Steroids

authors： Monder C,Lakshmi V

更新日期：1988-11-01 00:00:00

abstract：:While the neuroprotective benefits of estrogen and progesterone in critical illness are well established, the data regarding the effects of androgens are conflicting. Surgical repair of congenital heart disease is associated with significant morbidity and mortality, but there are scant data regarding the postoperative...

journal_title：Steroids

authors： Heckmann M,d'Uscio CH,de Laffolie J,Neuhaeuser C,Bödeker RH,Thul J,Schranz D,Frey BM

更新日期：2014-03-01 00:00:00

abstract：:Androgen-binding protein/sex hormone-binding globulin (ABP/SHBG) is an extracellular carrier protein that binds androgens and estrogens with high affinity. In the adult, ABP/SHBG is thought to function in the male reproductive system and the general circulation in both sexes to modulate the actions of sex steroids. Th...

journal_title：Steroids

authors： Becchis M,Sullivan PM,Ordronneau P,Petrusz P,Joseph DR

更新日期：1996-07-01 00:00:00

abstract：:A cytochrome P450-deficient mutant of the pathogenic fungus, Candida albicans, which accumulates exclusively 14 alpha-methylsterols in place of the normal end product sterol, ergosterol, was examined for alterations in membrane fluidity by electron paramagnetic resonance. The results using four nitroxyl spin labels in...

journal_title：Steroids

authors： Lees ND,Kleinhans FW,Broughton MC,Pennington DE,Ricker VA,Bard M

更新日期：1989-03-01 00:00:00

abstract：:Aspergillus niger, one of fungal species most frequently used for experimental and industrial-scale biotransformations of various organic compounds, is generally known to transform steroids at 16β position. In this work, application of the strain A. niger KCH910 to bioconversion of dehydroepiandrosterone (DHEA), andro...

journal_title：Steroids

authors： Świzdor A,Panek A,Milecka-Tronina N

更新日期：2017-10-01 00:00:00

abstract：:4- to 8-cell mouse PIE's (preimplantation embryos) were cultured in vitro for 48 hr in media containing various concentrations of the anti-estrogen CI-628. Graded effects of the various concentrations were observed with the highest concentration (1.5 microng/ml) being 100% effective in blocking development to the blas...

journal_title：Steroids

authors： Gupta JS,Dey SK,Dickmann Z

更新日期：1977-03-01 00:00:00

abstract：:The four diastereomeric 3,16-diacetates 6, 9, 10 and 11 were prepared from gitoxin 1 by the routes shown in Schemes 1 and 2 and tested for inotropic activity in the isolated guinea-pig atrial preparation. In line with earlier findings in the digitoxigenin series, derivatives with a 3 alpha-acetoxy function, viz 9 and ...

journal_title：Steroids

authors： Humber DC,Jones PS,Phillipps GH

更新日期：1985-01-01 00:00:00

abstract：:Smith-Lemli-Opitz syndrome (SLOS) is a malformation syndrome associated with 7-dehydrocholesterol (7DHC) 7-reductase deficiency. Although SLOS can be detected in an affected fetus before midpregnancy by measurement of 7DHC levels in amniotic fluid or chorionic villus cells, a noninvasive, more routine method is needed...

journal_title：Steroids

authors： Shackleton CH,Roitman E,Kratz LE,Kelley RI

更新日期：1999-07-01 00:00:00

abstract：:Estrogen levels in breast tumors of post-menopausal women are as much as 10 times higher than estrogen levels in plasma, presumably due to in situ formation of estrogen. The major source of estrogen in breast cancer cells may be conversion of estrone sulfate to estrone by the enzyme estrone sulfatase. Thus, inhibitors...

journal_title：Steroids

authors： Li PK,Chu GH,Guo JP,Peters A,Selcer KW

更新日期：1998-07-01 00:00:00

abstract：:The incorporation of the heterocycles into the steroid nucleus has been recognized as a useful strategy to develop new steroidal agents for disease treatment. Representative examples are abiraterone and galeterone, which are presently used in clinic for the treatment of advanced prostate cancers. Herein we have develo...

journal_title：Steroids

authors： Yang YT,Du S,Wang S,Jia X,Wang X,Zhang X

更新日期：2019-11-01 00:00:00

abstract：:The kinetics of 11 beta-hydroxysteroid dehydrogenase (11HSD) catalyzing the interconversion of cortisol (F) and cortisone (E) were compared in vitro following incubation of homogenates of human (N = 7) and baboon (N = 2) placenta. In both species, enzyme activity catalyzing the conversion of F to E was associated with...

journal_title：Steroids

authors： Pepe GJ,Albrecht ED

更新日期：1984-09-01 00:00:00

abstract：:A significant dose-response relationship between gonadotropin-releasing hormone (GnRH) and time to luteinizing hormone (LH) peak, peak serum LH and total serum LH was obtained in prepubertal Holstein heifers (28 weeks of age) (Experiment 1). For the second experiment, the effect of steroid feedback on the anterior pit...

journal_title：Steroids

authors： Swanson LV,McCarthy SK

更新日期：1986-02-01 00:00:00

abstract：:The high concentrations of dehydroepiandrosterone sulfate and pregnenolone sulfate in the mammalian brain, despite the blood-brain barrier's impermeability to these compounds, and the apparent independence of these concentrations from those in plasma prompted us to investigate whether enzymatic sulfation of dehydroepi...

journal_title：Steroids

authors： Rajkowski KM,Robel P,Baulieu EE

更新日期：1997-05-01 00:00:00

abstract：:Estrogen results in the suppression of experimental allergic encephalomyelitis (EAE), a frequently used experimental animal model of multiple sclerosis (MS). The mechanism by which estrogen acts in diseases with an autoimmune background is less clear. Here, we used splenic dendritic cells (DC) from the Lewis rats EAE ...

journal_title：Steroids

authors： Xiao BG,Liu X,Link H

更新日期：2004-09-01 00:00:00

abstract：:Thyroid hormone receptors (TRs) are nuclear receptors that are activated by thyroid hormone ligands and co-regulator proteins. Two receptor subtypes, TRalpha and TRbeta, have been suggested to play a role in numerous physiological functions. However, specificity of receptor subtype function and co-regulator interactio...

journal_title：Steroids

authors： Koury EJ,Pawlyk AC,Berrodin TJ,Smolenski CL,Nagpal S,Deecher DC

更新日期：2009-02-01 00:00:00

abstract：:In this study, effects of estradiol, progesterone and genistein on uterine aquaporin (AQP)-1, 2, 5 and 7 expression were investigated in sex-steroid deficient state which could help to elucidate the mechanisms underlying uterine fluid volume changes that were reported under these hormone and hormone-like compound infl...

journal_title：Steroids

authors： Chinigarzadeh A,Muniandy S,Salleh N

更新日期：2016-11-01 00:00:00

abstract：:CGS 16949A inhibited the conversion of [4-14C]androstenedione (A) to [4-14C]estrone by human placental microsomes in a competitive manner (Ki = 1.6 nM). Aminoglutethimide, also a competitive inhibitor, had a Ki = 0.7 microM in this assay system. The Km for the aromatization of A was 0.11 microM. Using ovarian microsom...

journal_title：Steroids

authors： Steele RE,Mellor LB,Sawyer WK,Wasvary JM,Browne LJ

更新日期：1987-07-01 00:00:00

abstract：:20S-hydroxyvitamin D3 (20S-(OH)D3), an in vitro product of vitamin D3 metabolism by the cytochrome P450scc, was recently isolated, identified and shown to possess antiproliferative activity without inducing hypercalcemia. The enzymatic production of 20S-(OH)D3 is tedious, expensive, and cannot meet the requirements fo...

journal_title：Steroids

authors： Li W,Chen J,Janjetovic Z,Kim TK,Sweatman T,Lu Y,Zjawiony J,Tuckey RC,Miller D,Slominski A

更新日期：2010-12-01 00:00:00

abstract：:Formestane (F, androst-4-en-4-ol-3,17-dione) is an irreversible aromatase inhibitor with the ability to suppress the estrogen production from anabolic steroids. Consequently, F is mentioned on the World Anti-Doping Agency (WADA) prohibited list and because studies have shown that F is produced endogenously in small am...

journal_title：Steroids

authors： Polet M,Van Renterghem P,Van Gansbeke W,Van Eenoo P

更新日期：2013-11-01 00:00:00

abstract：:To reduce the variability in estradiol (E2) testing and to assure better patient care, standardization of E2 measurements has been recommended. This study aims to assess the accuracy and variability of E2 measurements performed by 11 routine immunological methods and 6 mass spectrometry methods using single donor seru...

journal_title：Steroids

authors： Vesper HW,Botelho JC,Vidal ML,Rahmani Y,Thienpont LM,Caudill SP

更新日期：2014-04-01 00:00:00

abstract：:Morphologic changes at the interface of rat endometrial luminal epithelial cells and the stromal cells immediately adjacent were examined and correlated with hypertrophy of the epithelial cells during estradiol (E2) infusion (1 microgram E2/24 h). While the lamina densa in castrate endometrium was thread-like, it beca...

journal_title：Steroids

authors： Lavia LA,Larson BA

更新日期：1991-03-01 00:00:00

abstract：:Novel deoxycholic acid (DCA) derivatives were stereoselectively synthesised with -OH and -CH2SR moieties at the C-3 position, where R was a substituted aryl [2-aminophenyl (8) or 4-chlorophenyl (9)] or hetaryl [1-methylimidazolyl (5), 1,2,4-triazolyl (6), 5-amino-1,3,4-thiadiazolyl (7), pyridinyl (10) or pyrimidinyl (...

journal_title：Steroids

authors： Popadyuk II,Markov AV,Morozova EA,Babich VO,Salomatina OV,Logashenko EB,Zenkova MA,Tolstikova TG,Salakhutdinov NF

更新日期：2017-11-01 00:00:00

abstract：:The synthesis of 13C-labeled steroid hormones is reviewed. Two general approaches are highlighted: partial synthesis in which part of the steroid nucleus is replaced with 13C-labeled synthons, and total synthesis. Examples from both approaches, leading to (3-(3)C)-, (4-(13)C)-, (3,4-(13)C2)-, and (1,2,3,4-(13)C4)- lab...

journal_title：Steroids

authors： Zomer G,Stavenuiter JF

更新日期：1990-10-01 00:00:00

abstract：:Saturation analysis of the binding of [3H]dexamethasone [( 3H]DEX) to ammonium sulfate precipitates (ASPs) confirmed the presence of a limited-capacity, high-affinity binder in human adipose tissue cytosols. Various non-radioactive steroids competed with [3H]DEX for binding to the ASPs in the following sequence: dexam...

journal_title：Steroids

authors： Miller LK,Kral JG,Strain GW,Zumoff B

更新日期：1987-06-01 00:00:00

abstract：:Incubation of aldosterone with confluent layers of A6 (toad kidney) cells leads to its hydroxylation at the 6 beta-position. 6 beta-Hydroxyaldosterone is the major metabolite when the incubation is carried out at pH 6.8, whereas the product comprises 6 beta-hydroxy-17-isoaldosterone accompanied by some 6 beta-hydroxya...

journal_title：Steroids

authors： Morris DJ,Latif SA,Conca TJ,Wei LT,Watlington CO,Kirk DN,Toms HC,Shackleton CH

更新日期：1990-11-01 00:00:00

abstract：:A sensitive accurate assay for the placental microsomal 3 beta-hydroxysteroid dehydrogenase (E.C.1.1.1.51) has been developed using tritiated substrates. Kinetic analysis of the enzyme with 3 beta-hydroxy-5-androsten-17-one and 3 beta-hydroxy-5-pregnen-20-one indicates that the apparent Km values for these substrates ...

journal_title：Steroids

authors： Gibb W

更新日期：1979-04-01 00:00:00