Kinetic analysis of the placental microsomal 3 beta-hydroxysteroid dehydrogenase activity.

abstract：:By analyzing the steroidal content of fresh whole plants of Tacca subflabellata (Taccaceae), we isolated one sapogenin and eight glycosides with four kinds of steroidal skeletons including four new glycosides, named taccasubosides A-D (1-4), together with five known compounds. Among them, compound 1 is the first penta...

journal_title：Steroids

authors： Li L,Ni W,Li XR,Hua Y,Fang PL,Kong LM,Pan LL,Li Y,Chen CX,Liu HY

更新日期：2011-09-01 00:00:00

abstract：:Evidence showed that the stress hormone corticosterone (CORT) injection resulted in dysregulation of the hypothalamic-pituitary-adrenal (HPA) axis implicated in major depressive disorder. Magnolol, main constituent identified in the barks of Magnolia officinalis, exerted antidepressant effects in a rat model of depres...

journal_title：Steroids

authors： Bai Y,Song L,Dai G,Xu M,Zhu L,Zhang W,Jing W,Ju W

更新日期：2018-07-01 00:00:00

abstract：:CGS 16949A inhibited the conversion of [4-14C]androstenedione (A) to [4-14C]estrone by human placental microsomes in a competitive manner (Ki = 1.6 nM). Aminoglutethimide, also a competitive inhibitor, had a Ki = 0.7 microM in this assay system. The Km for the aromatization of A was 0.11 microM. Using ovarian microsom...

journal_title：Steroids

authors： Steele RE,Mellor LB,Sawyer WK,Wasvary JM,Browne LJ

更新日期：1987-07-01 00:00:00

abstract：:A series of chemiluminescent 17beta-estradiol probes were synthesized. Relative equilibrium dissociation constants (K(D)) for the interaction of an anti-E(2) Fab fragment for the probes in solution were evaluated using a single E(2)-analog biosensor surface on a BIAcore surface plasmon resonance instrument. The result...

journal_title：Steroids

authors： Adamczyk M,Chen YY,Gebler JC,Johnson DD,Mattingly PG,Moore JA,Reddy RE,Wu J,Yu Z

更新日期：2000-06-01 00:00:00

abstract：:Glucocorticoid receptor binding was compared in liver cytosol preparations from spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats using homologous displacement of [3H]dexamethasone. At 5 degrees C, there was no difference in receptor binding affinity or concentration between strains for dexamethasone, ...

journal_title：Steroids

authors： Panarelli M,Holloway CD,Barr AB,Fraser R,Kenyon CJ

更新日期：1995-01-01 00:00:00

abstract：:The purpose of this study was to investigate the influence of plasma steroid-binding proteins on androgen metabolism in intact leukocytes prepared from normal male and female blood samples. Leukocyte preparations were incubated for 24 h at 37 degrees C with either labeled or unlabeled testosterone (T), 5 alpha-dihydro...

journal_title：Steroids

authors： Déchaud H,Goujon R,Claustrat F,Boucherat M,Pugeat M

更新日期：1995-02-01 00:00:00

abstract：:Inflammatory cytokines interleukin-1 (IL-1) and tumor necrosis factor-α (TNF-α) regulate the activity of the hypothalamo-pituitary-adrenal (HPA) axis at several levels. Although hypothalamic CRH secretion may be the primary mechanism by which these cytokines activate the HPA axis, IL-1 expression is increased within t...

journal_title：Steroids

authors： Tkachenko IV,Jääskeläinen T,Jääskeläinen J,Palvimo JJ,Voutilainen R

更新日期：2011-09-01 00:00:00

abstract：:17-Hydroxyprogesterone is a well-known precursor of androstenedione in adrenal biosynthesis. This study using sheep adrenal incubations demonstrates that 11-deoxycortisol, the precursor of cortisol synthesis, also can be a precursor of androstenedione. Indeed, our data show that androstenedione synthesis is negatively...

journal_title：Steroids

authors： Auzéby A,Bogdan A,Touitou Y

更新日期：1991-01-01 00:00:00

abstract：:Estrogen is an important atheroprotective molecule that causes the rapid dilation of blood vessels by stimulating endothelial nitric oxide synthase (eNOS). There is also evidence that estrogen modulates airway epithelial NO production, thereby potentially affecting bronchial hyperresponsiveness. Studies in cultured en...

journal_title：Steroids

authors： Shaul PW

更新日期：1999-01-01 00:00:00

abstract：:While the neuroprotective benefits of estrogen and progesterone in critical illness are well established, the data regarding the effects of androgens are conflicting. Surgical repair of congenital heart disease is associated with significant morbidity and mortality, but there are scant data regarding the postoperative...

journal_title：Steroids

authors： Heckmann M,d'Uscio CH,de Laffolie J,Neuhaeuser C,Bödeker RH,Thul J,Schranz D,Frey BM

更新日期：2014-03-01 00:00:00

abstract：:Steroidal nucleoside analogs were synthesized starting from testosterone. By reduction of the oxime of 17 beta-hydroxy-androst-4-en-3-one (testosterone), a mixture of the two amino epimers of C-3 were obtained. The 3 alpha-amino-androst-4-en-17 beta-ol was crystallized in 73% yield and coupled with 5-amino-4,6-dichlor...

journal_title：Steroids

authors： Gelpi ME,Cadenas RA,Mosettig J,Zuazo BN

更新日期：2002-03-01 00:00:00

abstract：:It is known that follicle-stimulating hormone (FSH) and insulin stimulate estradiol secretion from cultured non-luteinizing granulosa cells. The interaction between these hormones is less well understood. Granulosa cells from small (2-4 mm) bovine follicles were cultured in serum-free medium to determine if cytochrome...

journal_title：Steroids

authors： Bhatia B,Price CA

更新日期：2001-06-01 00:00:00

abstract：:Fingernail clipping is expected to be a specimen for steroid testing, because it has several advantages over blood; i.e., noninvasive collection, ease of storage, portability and handling, and possibility for an assessment of the steroid status over a relatively long and retrospective time window. In this study, we ex...

journal_title：Steroids

authors： Higashi T,Yamagata K,Kato Y,Ogawa Y,Takano K,Nakaaze Y,Iriyama T,Min JZ,Ogawa S

更新日期：2016-05-01 00:00:00

abstract：:The in vivo retention of 3-H-testosterone, dihydrotestosterone (DHT), 3alpha-androstanediol (3alpha-DIOL), 3beta-DIOL, androstenedione, progesterone and cortisol by renal cytoplasm and nuclei of male and female mice was studied. Testosterone was the major androgen isolated from cytoplasm and nuclei following testoster...

journal_title：Steroids

authors： Bullock LP,Bardin CW

更新日期：1975-01-01 00:00:00

abstract：:The syntheses of 15beta-carboxyethylmercapto-5alpha-dihydrotestosterone, 15beta-carbosyethylmercapto-5alpha-androstane-3beta, 17beta-diol and 15beta-carboxyethylmercapto-5alpha-androstane-3alpha, 17beta-diol and the preparation of their bovine serum albumin (BSA) conjugates are described. These conjugates were employe...

journal_title：Steroids

authors： Rao PN,Khan AH,Moore PH Jr

更新日期：1977-02-01 00:00:00

abstract：:Dehydroepiandrosterone (DHEA) is a multifunctional steroid with a broad range of biological effects in humans and animals. DHEA can be converted to multiple oxygenated metabolites in the brain and peripheral tissues. The mechanisms by which DHEA exerts its effects are not well understood. However, evidence that the ef...

journal_title：Steroids

authors： El Kihel L

更新日期：2012-01-01 00:00:00

abstract：:Glucocorticoids (GCs) are essential steroid hormones that regulate numerous metabolic and homeostatic functions in almost all physiological systems. Synthetic glucocorticoids are among the most commonly prescribed drugs for the treatment of various conditions including autoimmune, allergic and inflammatory diseases. G...

journal_title：Steroids

authors： Sulaiman RS,Kadmiel M,Cidlowski JA

更新日期：2018-05-01 00:00:00

abstract：:Previous studies have demonstrated a persistent rise in serum cortisol concentrations after cardiac surgery. To further investigate this finding and to evaluate the effect of hemodilution that occurs with the onset of cardiopulmonary bypass (CPB), concentrations of cortisol-binding globulin (CBG), total and unbound co...

journal_title：Steroids

authors： Roth-Isigkeit AK,Dibbelt L,Schmucker P

更新日期：2000-09-01 00:00:00

abstract：:3 beta-Hydroxy-5-androsten-17-one is converted to 5-androstene-3, 17-dione by rat liver alcohol dehydrogenase (ADH). We have reported on the purity of the enzyme which is eluted with pyrazole as a single homogeneous protein using an AMP-agarose affinity column. Rat liver ADH can oxidize hydroxyl groups not only at 3 b...

journal_title：Steroids

authors： Knutson VP,Ungar F

更新日期：1982-11-01 00:00:00

abstract：:In an endeavour to develop potent anti-tumor agents from diosgenin, a series of C-6 derived 1,2,3-triazolyl derivatives were designed and synthesized by employing Cu(I) catalyzed Huisgen 1,3-dipolar cycloaddition reaction of novel azides - (22R,25R)-6β-azidospirostan-3β,5α-diol and 6β-azido-7α-hydroxyspirosta-1,4-dien...

journal_title：Steroids

authors： Mironov ME,Oleshko OS,Pokrovskii MA,Rybalova TV,Pechurov VK,Pokrovskii AG,Cheresis SV,Mishinov SV,Stupak VV,Shults EE

更新日期：2019-11-01 00:00:00

abstract：:A series of steroidal and nonsteroidal Michael acceptors that represent reaction products for 3 alpha-hydroxysteroid dehydrogenase were synthesized and evaluated as potential enzyme-generated inactivators. Introduction of exocyclic olefins either at C-2 or C-6 produced inhibitors with high affinity for the enzyme (0.0...

journal_title：Steroids

authors： Penning TM,Thronton R,Ricigliano JW

更新日期：1991-08-01 00:00:00

abstract：:A time course study to measure adrenal cortical function was undertaken for the period prior to the development of hypertension until the onset of hypertension in the adrenal-regeneration hypertension (ARH) model. Quiescent rat kills were used so that all adrenal cortical parameters investigated would reflect basal or...

journal_title：Steroids

authors： Bergon L,Gallant S,Brownie AC

更新日期：1975-03-01 00:00:00

abstract：:5 alpha-(17R,20R)-14,15-Secocholestane (12) was synthesized from cholesterol (1) in 12 steps. The key intermediate, 5 alpha-cholest-14-en-3 beta-yl acetate (4), underwent ozonization, reduction, hydrolysis, and oxidation to provide 5 alpha-14,15-secocholesta-3,14,15-trione (8). One of the Clemmensen reduction products...

journal_title：Steroids

authors： Li TS,Li YL,Liang XT

更新日期：1990-06-01 00:00:00

abstract：:A radioimmunoassay technique has been developed for the measurement of cortisol in a single methylene chloride extract of human plasma without chromatography. The antiserum, obtained by immunizing rabbits with cortisol-3-carboxymethyl-oxime conjugated to bovine serum albumin, had a high affinity (KA = 1.8 X 10(9) 1/mo...

journal_title：Steroids

authors： Dash RJ,England BG,Midgley AR Jr,Niswender GD

更新日期：1975-11-01 00:00:00

abstract：:Steroids have been shown to have multiple effects on the nervous system including neuroprotective activities, and they have the potential to be used for the treatment of neurodegenerative diseases. In this current study, we tested the hypothesis that the marine steroid 24-methylenecholestane-3β,5α,6β,19-tetraol (Tetro...

journal_title：Steroids

authors： Leng T,Liu A,Wang Y,Chen X,Zhou S,Li Q,Zhu W,Zhou Y,Su X,Huang Y,Yin W,Qiu P,Hu H,Xiong ZG,Zhang J,Yan G

更新日期：2016-01-01 00:00:00

abstract：:Morphologic changes at the interface of rat endometrial luminal epithelial cells and the stromal cells immediately adjacent were examined and correlated with hypertrophy of the epithelial cells during estradiol (E2) infusion (1 microgram E2/24 h). While the lamina densa in castrate endometrium was thread-like, it beca...

journal_title：Steroids

authors： Lavia LA,Larson BA

更新日期：1991-03-01 00:00:00

abstract：:The concentration of testosterone (T) in the peripheral plasma of laboratory mice is extremely variable. This variability is already evident at 20--25 days of age and is not eliminated by brief or chronic exposure to male or female mice, or by isolation. The variation in T levels in plasma samples collected from the s...

journal_title：Steroids

authors： Bartke A,Dalterio S

更新日期：1975-12-01 00:00:00

abstract：:Contrary to the long-held postulate of steroid-hormone binding globulin action, these protein carriers of steroids are major players in steroid actions in the body. This manuscript will focus on our work with sex hormone binding globulin (SHBG) and corticosteroid binding globulin (CBG) and demonstrate how they are act...

journal_title：Steroids

authors： Caldwell JD,Jirikowski GF

更新日期：2014-03-01 00:00:00

abstract：:The steroid hormone dehydroepiandrosterone (DHEA) has beneficial effects on vascular function, survival of neurons, and fatty acid metabolism. However, a specific receptor for DHEA has not been identified to date. Here, we describe the synthesis of a photoreactive DHEA derivative (Photo-DHEA). In Photo-DHEA, typical c...

journal_title：Steroids

authors： Waschatko G,Kojro E,Zahnow M,Gehrig-Burger K

更新日期：2011-04-01 00:00:00

abstract：:The uterine milk (UTM) proteins are the major progesterone-regulated proteins secreted by the sheep uterus during pregnancy. Recently, proteins related to the UTM proteins have been identified in uterine secretions of the pregnant cow and sow. The present objective was to determine the time course for induction of the...

journal_title：Steroids

authors： Leslie MV,Hansen PJ

更新日期：1991-12-01 00:00:00