Abstract：
:Fingernail clipping is expected to be a specimen for steroid testing, because it has several advantages over blood; i.e., noninvasive collection, ease of storage, portability and handling, and possibility for an assessment of the steroid status over a relatively long and retrospective time window. In this study, we examined whether there is a difference in the nail contents between the right and left hands for five steroids [glycochenodeoxycholic acid (GCDCA), taurochenodeoxycholic acid (TCDCA), dehydroepiandrosterone sulfate (DHEAS), testosterone (TST) and cortisol (CRT)] using newly developed liquid chromatography/electrospray ionization-tandem mass spectrometry methods. The nail contents between the hands were significantly different for GCDCA, TCDCA and DHEAS, whereas those of TST and CRT only slightly differed. These results might be due to the difference in the binding affinity of each steroid for the nail keratin. The relatively hydrophilic steroids, GCDCA, TCDCA and DHEAS, may be lost from nails in daily life due to their low affinity for keratin, which would produce differences in the nail contents between the hands. Thus, the fingernail GCDCA, TCDCA and DHEAS contents may be influenced by factors other than the disease; the nail analysis is inefficient in the diagnosis of the disease associated with these steroids. On the other hand, the nail analysis looks promising for evaluation of the status of TST and CRT, which are lipophilic and inferred to be tightly bound to the keratin. In fact, the nail TST content showed a significant sex difference, just like its serum/plasma concentration.
journal_name
Steroidsjournal_title
Steroidsauthors
Higashi T,Yamagata K,Kato Y,Ogawa Y,Takano K,Nakaaze Y,Iriyama T,Min JZ,Ogawa Sdoi
10.1016/j.steroids.2016.02.013subject
Has Abstractpub_date
2016-05-01 00:00:00pages
60-5eissn
0039-128Xissn
1878-5867pii
S0039-128X(16)00044-1journal_volume
109pub_type
杂志文章相关文献
STEROIDS文献大全abstract：:Sedentary lifestyle and over-nutrition are the main causes of obesity and type 2 diabetes (T2D). However, the same causes are major triggers of hypogonadism. Many T2D patients show low testosterone levels while hypogonadal men seem to be prone to become diabetic. Testosterone plays a major role in the regulation of mu...
journal_title：Steroids
pub_type： 杂志文章,评审
doi：10.1016/j.steroids.2018.08.002
更新日期：2018-10-01 00:00:00
abstract：:Sleep apnea is a disorder, which increasingly affects people worldwide. Whether the associated hypoxic events during sleep are central or obstructive in origin, the end result is excessive daytime sleepiness and an increased risk for several comorbidities, such as cardiovascular and neurodegenerative disorders. Sleep ...
journal_title：Steroids
pub_type： 杂志文章,评审
doi：10.1016/j.steroids.2017.12.006
更新日期：2018-05-01 00:00:00
abstract：:A series of clickable bile acid-nucleosides conjugates linked directly or via amino acid linker were synthesized, and characterized by spectroscopic techniques such as 1H NMR, 13C NMR, FT-IR, HRMS and HPLC. The synthesized compounds 6a-p were screened for their in vitro anticancer property against a panel of three can...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/j.steroids.2018.09.006
更新日期：2018-11-01 00:00:00
abstract：OBJECTIVE:To evaluate the role of physiologic levels of androgens and their precursors in the regulation of body composition, energy and substrate metabolism and aerobic capacity in healthy, cycling, premenopausal women. EXPERIMENTAL:We evaluated 30 young (27±1 year) premenopausal, non-obese (23±0.5 kg/m(2)), normally...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/j.steroids.2011.06.001
更新日期：2011-11-01 00:00:00
abstract：:17beta-estradiol exerts an antiapoptotic action in skeletal muscle cells through extranuclear ERalpha and beta. This protective action, mainly involves a non-genomic mechanism of ERK1/2 and PI3K/Akt activation and BAD phosphorylation. ERbeta plays a major role in the inhibition of apoptosis by 17beta-estradiol at the ...
journal_title：Steroids
pub_type： 杂志文章,评审
doi：10.1016/j.steroids.2007.12.027
更新日期：2008-10-01 00:00:00
abstract：:Spectroscopic examination of purified extracts of the rumen content of sheep intoxicated by Brachiaria decumbens revealed the presence of two spirostanes, identified as epi-sarsasapogenin and epi-smilagenin. Sarsasapogenone was obtained by the oxidation of sarsasapogenin. The reduction of sarsasapogenone using lithium...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/0039-128x(93)90043-m
更新日期：1993-08-01 00:00:00
abstract：:We describe a convenient and stereoselective route to the synthesis of 27-hydroxycholesterol. Also its radiolabeled analog, 22, 23 di [(3)H]-27-hydroxycholesterol with high specific radioactivity (55 Ci/mmol) was synthesized by this method. Julia condensation of steroidal 22-sulfone with aldehyde, led to the addition ...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/s0039-128x(00)00099-4
更新日期：2000-07-01 00:00:00
abstract：:Human placental estradiol 17 beta-dehydrogenase (E.C. 1.1.1.62) was inactivated at pH 6.3 by 3-bromo [2'-14C] acetoxy-1,3,5(10) estratrien-17-one, a know substrate. The affinity-alkylated enzyme was then hydrolyzed by trypsin. Radioactive peptides were initially isolated by gel filtration and identified according to w...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/0039-128x(82)90084-8
更新日期：1982-02-01 00:00:00
abstract：:Human progesterone receptor (PR) contains a polyproline motif in the amino-terminal domain that interacts with the SH3 domain of Src and mediates rapid activation of c-Src and downstream MAPK (Erk-1/-2) independent of the transcriptional activity of PR. Forcedly target PR to different locations in the cell by use of m...
journal_title：Steroids
pub_type： 杂志文章,评审
doi：10.1016/j.steroids.2008.01.010
更新日期：2008-10-01 00:00:00
abstract：:The accurate analysis of trace components in complex biological matrices requires the use of reliable internal standards. For liquid chromatography/mass spectrometry analysis, the stable isotope-labeled analogues of the analyte molecules are the most appropriate internal standards. In this paper the synthesis of the 3...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/j.steroids.2012.08.007
更新日期：2012-11-01 00:00:00
abstract：:Inhibition of 5alpha-reduction of testosterone by an anti-androgen TSAA-291 (16beta-ethyl-17beta-hydroxy-4-estren-3-one) was studied in rat ventral prostates and the metabolic conversion of 3H-TSAA-291 was examined both in vitro and in vivo. In the in vitro experiment using nuclear 5alpha-reductase of the prostate, 5a...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/0039-128x(81)90021-0
更新日期：1981-07-01 00:00:00
abstract：:Membrane-initiated cellular responses to steroids include modulation of ion channel activities via signal transduction pathways. However, the molecular mechanisms involved in nongenomic actions remain only partially understood. Our research has focused on the rapid effects of 1alpha,25(OH)(2) Vitamin D(3) [1,25D] on L...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/j.steroids.2005.09.012
更新日期：2006-04-01 00:00:00
abstract：:We report the synthesis of 3 beta,15 beta,17 alpha-trihydroxy-5-pregnen-20- one (1) from 3 beta,15 beta-dihydroxy-5,16-pregnadien-20-one (11) in 7 steps using boronate derivatives as a means of protecting the 17,20-glycol side-chain of steroid intermediates. 16 alpha,17 alpha-Epoxy-3 beta,15 beta-dihydroxy-5- pregnen-...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/0039-128x(95)00169-q
更新日期：1996-01-01 00:00:00
abstract：:The biosynthesis of progesterone from [3H]pregnenolone was curvilinear over a 6 h time course in human placenta cytotrophoblasts and in human placenta choriocarcinoma cells (JEG-3 cells). Mass measurements determined independently by radioimmunoassay indicate that the progesterone synthesized by cytotrophoblasts (21.0...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/0039-128x(88)90043-8
更新日期：1988-05-01 00:00:00
abstract：:The role played by progestogens in modulating Schwann cell pathophysiology is well established. Progestogens exert their effects in these cells through both classical genomic and non-genomic mechanisms, the latter mediated by the GABA-A receptor. However, there is evidence that other receptors may be involved. Membran...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/j.steroids.2017.09.009
更新日期：2019-02-01 00:00:00
abstract：:Reaction kinetics of the aromatase enzyme and of a new nonsteroidal aromatase inhibitor, R 76 713 (6-[(4-chlorophenyl)(1H-1,2,4-triazol-1-yl)-methyl]-1-methyl-1H- benzotriazole), were studied in ovarian homogenates obtained from pregnant mare's serum gonadotropin (PMSG)-injected female Wistar rats. The Km (Michaelis c...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/0039-128x(90)90027-9
更新日期：1990-02-01 00:00:00
abstract：:Synthesis, biochemical and biological testing of the first carborane derivatives of estrogens are described. Estrone 3-carboranylmethyl ether was synthesized in two steps from estrone. Reduction of estrone 3-carboranylmethyl ether with sodium borohydride provided estradiol-17 beta 3-carboranylmethyl ether. Enzyme kine...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/s0039-128x(81)80020-7
更新日期：1981-02-01 00:00:00
abstract：:The reaction of 23-oxotigogenin acetate with TMSOTf in THF afforded the corresponding bisnorcholanic lactone in 60% yield. The analogous reactions carried out in dichloromethane or benzene gave the rearranged products--the isomeric spirostanic ketone (10-15%) and bisfuran (40-42%). Similar products were also obtained ...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/j.steroids.2009.02.010
更新日期：2009-08-01 00:00:00
abstract：:Ru(II) steroidal metal complexes were synthesized by the reaction of dichlorodicarbonyl ruthenium(II) [Ru(CO)2Cl2]n with Steroidal thiosemicarbazones. Coordination via the thionic sulfur and the azomethine nitrogen atom of the thiosemicarbazone to the Ru(II) metal. Steroidal thiosemicarbazone derivatives were obtained...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/j.steroids.2017.05.001
更新日期：2017-08-01 00:00:00
abstract：:This review lays out the evidence for the role of E2 in homeostatic and hedonic feeding across several species. While significant effort has been expended on homeostatic feeding research, more studies for hedonic feeding need to be conducted (i.e. are there increases in meal size and enhanced motivation to natural foo...
journal_title：Steroids
pub_type： 杂志文章,评审
doi：10.1016/j.steroids.2017.11.011
更新日期：2018-05-01 00:00:00
abstract：:This article has been withdrawn at the request of the Publisher and the Editor-in-Chiefs. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy. ...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/j.steroids.2010.11.002
更新日期：2010-11-12 00:00:00
abstract：:Cell cycle regulator p21(CIP1) has controversial biological effects in breast cancer since in spite of its role as cell cycle inhibitor and promoter of cellular senescence, it also induces cell proliferation and chemoteraphy resistance. We here explored the molecular mechanisms involved in progestin regulation of p21(...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/j.steroids.2012.11.003
更新日期：2013-06-01 00:00:00
abstract：:The uterine milk (UTM) proteins are the major progesterone-regulated proteins secreted by the sheep uterus during pregnancy. Recently, proteins related to the UTM proteins have been identified in uterine secretions of the pregnant cow and sow. The present objective was to determine the time course for induction of the...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/0039-128x(91)90022-n
更新日期：1991-12-01 00:00:00
abstract：:Based on the fact that timosaponin A-III (TA-III) exhibits potent cytotoxic effects and has been considered as a potential anti-tumor agent, a range of novel sarsasapogenin derivatives 1, 2a-2g, 3, 4, 5, 6a-6g have been synthesized by a simple and facile synthetic route. The in vitro cytotoxic activity of these synthe...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/j.steroids.2014.09.007
更新日期：2015-01-01 00:00:00
abstract：:The formation of a horseradish peroxidase-testosterone conjugate for the enzyme-linked immunoassay of testosterone was investigated, using tritiated testosterone to follow the reaction. The formation of testosterone-3-(carboxymethyl) oxime-peroxidase by the mixed anhydride method was found to give a conjugate of high ...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/0039-128x(77)90021-6
更新日期：1977-05-01 00:00:00
abstract：:3 beta-Hydroxy-5-androsten-17-one is converted to 5-androstene-3, 17-dione by rat liver alcohol dehydrogenase (ADH). We have reported on the purity of the enzyme which is eluted with pyrazole as a single homogeneous protein using an AMP-agarose affinity column. Rat liver ADH can oxidize hydroxyl groups not only at 3 b...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/0039-128x(82)90079-4
更新日期：1982-11-01 00:00:00
abstract：:A series of chemiluminescent 17beta-estradiol probes were synthesized. Relative equilibrium dissociation constants (K(D)) for the interaction of an anti-E(2) Fab fragment for the probes in solution were evaluated using a single E(2)-analog biosensor surface on a BIAcore surface plasmon resonance instrument. The result...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/s0039-128x(00)00091-x
更新日期：2000-06-01 00:00:00
abstract：:Glucocorticoids (GCs) and progesterone have been employed as immunosuppressive agents during pregnancy for many years. Intracellular acidification by GCs is due to a rapid non-genomic inhibition of membrane Na(+)/H(+)-exchange 1 (NHE1) activity and is followed by immunosuppression of PHA-stimulated proliferation. NHE1...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/j.steroids.2016.01.004
更新日期：2016-07-01 00:00:00
abstract：:A series of novel 5α, 8α-endoperoxide steroidal hybrid derivatives containing isatin or indole substituents on the C-17 side chain were synthesized and characterized. The preliminary anti-proliferative activity of the compounds against HepG2, MCF-7, HT-29 and HeLa cell lines were investigated. Compounds 7g and 7l disp...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/j.steroids.2019.108471
更新日期：2020-01-01 00:00:00
abstract：:Using a whole cell suspension assay technique, we have examined both the specific binding of testosterone (T) and dihydrotestosterone (17 beta-hydroxy-5 alpha-androstan-3-one) (DHT) and the subsequent distribution of androgen binding activity within MCF-7 human breast cancer cells. We have observed that both androgens...
journal_title：Steroids
pub_type： 杂志文章
doi：10.1016/0039-128x(81)90078-7
更新日期：1981-10-01 00:00:00
