Abstract:
:Cell cycle regulator p21(CIP1) has controversial biological effects in breast cancer since in spite of its role as cell cycle inhibitor and promoter of cellular senescence, it also induces cell proliferation and chemoteraphy resistance. We here explored the molecular mechanisms involved in progestin regulation of p21(CIP1) expression. We also investigated the biological effects of p21(CIP1) in breast cancer cells. We found that the synthetic progestin medroxyprogesterone acetate (MPA) upregulates p21(CIP1) protein expression via c-Src, signal transducer and activator of transcription 3 (Stat3) and ErbB-2 phosphorylation. Notably, we also found that ErbB-2 nuclear function plays a key role in MPA-induction of p21(CIP1) expression. Interestingly, we determined that progestin drives p21(CIP1) transcriptional activation via a novel nonclassical transcriptional mechanism in which progesterone receptor is recruited along with Stat3 and ErbB-2 to a Stat3 binding site at p21(CIP1) promoter. Our findings revealed that ErbB-2 functions as a coactivator of Stat3 in progestin induction of p21(CIP1) transcriptional activation. Furthermore, we demonstrated that blockage of p21(CIP1) expression strongly inhibited in vitro and in vivo progestin-induced breast cancer cell proliferation. These results further support the hypothesis that according to cell context and type of stimulus, p21(CIP1) is capable of inducing cell cycle progression. Moreover, we provided evidence that Stat3 and nuclear ErbB-2 are key players in progestin-induced p21(CIP1) regulation.
journal_name
Steroidsjournal_title
Steroidsauthors
Diaz Flaqué MC,Vicario R,Proietti CJ,Izzo F,Schillaci R,Elizalde PVdoi
10.1016/j.steroids.2012.11.003subject
Has Abstractpub_date
2013-06-01 00:00:00pages
559-67issue
6eissn
0039-128Xissn
1878-5867pii
S0039-128X(12)00303-0journal_volume
78pub_type
杂志文章相关文献
STEROIDS文献大全abstract::It has been shown that stress or disease-induced increases in plasma corticosterone result in diminished testosterone secretion from the testes. This article reviews investigations from our laboratories that explore the role of 11 beta-hydroxysteroid dehydrogenase (11 beta-OHSD) in this process. It is proposed that th...
journal_title:Steroids
pub_type: 杂志文章,评审
doi:10.1016/0039-128x(94)90078-7
更新日期:1994-02-01 00:00:00
abstract::The peripheral conversion of testosterone (T) into androstenedione (delta 4) and dihydrotestosterone (DHT) were studied in pregnant (days 32, 48 and 67 post coitum) and non-pregnant anesthetized guinea-pigs by constant intravenous infusion of tritium labeled T. Endogenous levels were measured by radioimmunoassay, and ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(83)90157-5
更新日期:1983-08-01 00:00:00
abstract::Structural modifications of natural products with complex structures like steroids require great synthetic effort. A review of literature is presented on the chemistry of the steroidal hormone 17α-methyltestosterone that is approved by Food and Drug Administration (FDA) in the United States as an androgen for estrogen...
journal_title:Steroids
pub_type: 杂志文章,评审
doi:10.1016/j.steroids.2015.11.004
更新日期:2016-01-01 00:00:00
abstract::Estrogen is the key regulator of bone metabolism in both men and women. Menopause and the accompanying loss of ovarian estrogens are associated with declines in bone mineral density (BMD): 10-year cumulative loss was 9.1% at the femoral neck and 10.6%, lumbar spine. Estradiol concentrations also predict fractures. Tot...
journal_title:Steroids
pub_type: 杂志文章,评审
doi:10.1016/j.steroids.2014.12.010
更新日期:2015-07-01 00:00:00
abstract::Thyroid hormone receptors (TRs) are nuclear receptors that are activated by thyroid hormone ligands and co-regulator proteins. Two receptor subtypes, TRalpha and TRbeta, have been suggested to play a role in numerous physiological functions. However, specificity of receptor subtype function and co-regulator interactio...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2008.11.014
更新日期:2009-02-01 00:00:00
abstract::The mass spectral elimination of water in epimeric 1,3-diols of vitamin D3 (colecalciferol) series has been investigated. It was found that the mass spectra of these steroisomers differ sharply in the relative intensities of the ions M-H2O (m/e 382) and a-H2O (m/e +/- 34), where ion a (formed via formal cleavage of th...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(79)90039-4
更新日期:1979-05-01 00:00:00
abstract::This article has been withdrawn at the request of the Publisher and the Editor-in-Chiefs. The Publisher apologizes for any inconvenience this may cause. The full Elsevier Policy on Article Withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy. ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2010.11.002
更新日期:2010-11-12 00:00:00
abstract::Until recently, the idea that estradiol could affect cellular processes independent of nuclear estrogen receptors was often dismissed as artifact. This in spite of a large number of carefully controlled studies performed both within and outside the nervous system demonstrating estrogens regulate various intracellular ...
journal_title:Steroids
pub_type: 杂志文章,评审
doi:10.1016/j.steroids.2008.11.013
更新日期:2009-07-01 00:00:00
abstract::Dehydroepiandrosterone (DHEA) is an endogenous steroid hormone involved in a number of biological actions in humans and rodents, but its effects on renal tissue have not yet been fully understood. The aim of this study is to assess the effect of DHEA treatment on diabetic rats, mainly in relation to renal function and...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2011.02.006
更新日期:2011-05-01 00:00:00
abstract::Bile acid conjugates are found in human breast cyst fluid in average concentrations about 50-fold greater than those in blood. Because epidemiologic studies have linked colon and breast cancer and aberrant bile acid profiles are associated with colon cancer risk, we decided to study the influence of bile acid conjugat...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(97)00050-0
更新日期:1997-10-01 00:00:00
abstract::Dehydroepiandrosterone (DHEA) is a multifunctional steroid with a broad range of biological effects in humans and animals. DHEA can be converted to multiple oxygenated metabolites in the brain and peripheral tissues. The mechanisms by which DHEA exerts its effects are not well understood. However, evidence that the ef...
journal_title:Steroids
pub_type: 杂志文章,评审
doi:10.1016/j.steroids.2011.09.008
更新日期:2012-01-01 00:00:00
abstract::Enzyme immunoassays (EIA) are commonly utilized for the evaluation of androgens in biological fluids; however, careful consideration must be given to cross-reactivity with other endogenous sex-steroid hormones. Our purpose was to determine the validity of a commonly-utilized commercially-available dihydrotestosterone ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2013.08.013
更新日期:2013-12-11 00:00:00
abstract::Several derivatives of the arthropod moulting hormone have been synthesized which were coupled to AH Sepharose 4B yielding about 2 mumole ligand per g wet gel. As an indication of the suitability of the ligands for biological work the puff inducing capacity of their methyl esters was tested. The methyl ester of inokos...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(76)90088-x
更新日期:1976-04-01 00:00:00
abstract::By analyzing the steroidal content of fresh whole plants of Tacca subflabellata (Taccaceae), we isolated one sapogenin and eight glycosides with four kinds of steroidal skeletons including four new glycosides, named taccasubosides A-D (1-4), together with five known compounds. Among them, compound 1 is the first penta...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2011.04.003
更新日期:2011-09-01 00:00:00
abstract::A facile strategy for the annulation of 2,6-dicyanoaniline moiety to steroidal A/B-ring is described from base catalyzed and microwave-promoted reaction of steroidal 3-keto-2-hydroxymethylenes with malononitrile in high yields. The generality of the reaction has been extended to non-steroidal cyclic and aliphatic keto...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2010.02.009
更新日期:2010-06-01 00:00:00
abstract::Antiandrogens are widely used agents for the treatment of androgen dependent diseases as inhibitors of androgen receptors (AR) action. Although the precise mechanism of antiandrogen action is not yet elucidated, recent studies indicate the involvement of the structure of the ligand in relation with the nuclear co-repr...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2008.03.006
更新日期:2008-09-01 00:00:00
abstract::In an attempt to synthesize compounds with selective estrogen-receptor binding, fluoro- and amino-clomiphene were totally synthesized from benzyl chloride, and their estrogenic/antiestrogenic activity as well as that of some of their chemical intermediates was evaluated. The triazene prepared from the amino-clomiphene...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(86)90042-5
更新日期:1986-07-01 00:00:00
abstract::Biotransformation of an orally active contraceptive drug, drospirenone (1), by Cunninghamella elegans ATCC 36114 yielded four new metabolites, 6β,7β,15β,16β-dimethylene-3-oxo-14α-hydroxy-17α-pregn-4-ene-21,17-carbolactone (2), 6β,7β,15β,16β-dimethylene-3,11-dioxo-17α-pregn-4-ene-21,17-carbolactone (3), 6β,7β,15β,16β-d...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2017.07.010
更新日期:2017-10-01 00:00:00
abstract::The effect of cortisol and prostaglandin inhibitors on the growth and development of germinating mung bean, Vigna radiata L. Wilzek, cv. Jumbo was investigated. Cortisol, indomethacin, and a mixture of cortisol with aspirin, or benoxaprofen significantly increased radicle length and the number of lateral roots as comp...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(85)90052-2
更新日期:1985-08-01 00:00:00
abstract::Immature hypophysectomized rats were injected with PMS; some groups received hCG 48h later. The C17,20-lyase activity in the granulosa cells removed from the large preovulatory follicles was estimated by the amount of labelled acetic acid produced from 21 (14C) progesterone or 17-hydroxyprogesterone. 17 alpha-hydroxyl...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(83)90120-4
更新日期:1983-11-01 00:00:00
abstract::Boldenone is an anabolic-androgenic steroid that is prohibited in equine sports. Urine from the uncastrated male horse contains boldenone that is thought to be of endogenous origin and thus a threshold ('cut-off') concentration has been adopted internationally for free and conjugated boldenone to help distinguish case...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2019.03.011
更新日期:2019-06-01 00:00:00
abstract::A simplified, non-chromatographic method for aldosterone-gamma-lactone (2) radio-immunoassay is described, using a high titer aldosterone-gamma-lactone antibody. This method takes six working hours for completion; the blanks are below the sensitivity of the assay (0-10 pg), and the intra and interassay coefficient of ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(76)90004-0
更新日期:1976-11-01 00:00:00
abstract::An homogenate from cortical tissue of mare adrenals was incubated in the presence of tritiated pregnenolone. The (3H) androstenedione and the (3H) testosterone synthesized during the incubation were extracted, purified, and co-crystallized to constant specific activity in the presence of unlabeled carriers. The rate o...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/0039-128x(84)90033-3
更新日期:1984-02-01 00:00:00
abstract::The abundant expression of progesterone receptors (PR) in human meningiomas is well established. It is unknown, however, how PR expression is regulated, especially since estrogen receptors (ER) are virtually absent in these tumors. At the mRNA level, ER splice variants occur in meningioma but these appear not to be in...
journal_title:Steroids
pub_type: 杂志文章,评审
doi:10.1016/s0039-128x(00)00193-8
更新日期:2000-10-01 00:00:00
abstract::(20S)-[7,7,21,21-2H(4)]-3beta-(tert-Butyldimethylsilanyloxy)-20-methyl-pregn-5-en-21-ol, an intermediate for the preparation of deuterated isotopomers of sterols to be used as standards for biomedical studies, was prepared by reduction with dichloroaluminum deuteride of ethyl (20S)-3beta-(tert-butyldimethylsilanyloxy)...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(02)00172-1
更新日期:2003-02-01 00:00:00
abstract::Estrogen sulfamates are promising hormones by oral administration. Therefore, generally applicable and convenient methods for the multigram synthesis of these derivatives are desirable. Numerous estra-1,3,5(10)-trienes derived from estrone, estradiol. 14 alpha,15 alpha-methylenestradiol, ethinylestradiol, and estriol ...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(96)00200-0
更新日期:1996-12-01 00:00:00
abstract::In demyelinating or non-demyelinating neurodegenerative diseases, increased levels of 7-ketocholesterol (7KC), 7β-hydroxycholesterol (7β-OHC) and 24(S)-hydroxycholesterol (24S-OHC) can be observed in brain lesions. In 158N murine oligodendrocytes, 7KC triggers a complex mode of cell death defined as oxiapoptophagy, in...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2015.02.003
更新日期:2015-07-01 00:00:00
abstract::Synthesis, biochemical and biological testing of the first carborane derivatives of estrogens are described. Estrone 3-carboranylmethyl ether was synthesized in two steps from estrone. Reduction of estrone 3-carboranylmethyl ether with sodium borohydride provided estradiol-17 beta 3-carboranylmethyl ether. Enzyme kine...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(81)80020-7
更新日期:1981-02-01 00:00:00
abstract::Ru(II) steroidal metal complexes were synthesized by the reaction of dichlorodicarbonyl ruthenium(II) [Ru(CO)2Cl2]n with Steroidal thiosemicarbazones. Coordination via the thionic sulfur and the azomethine nitrogen atom of the thiosemicarbazone to the Ru(II) metal. Steroidal thiosemicarbazone derivatives were obtained...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/j.steroids.2017.05.001
更新日期:2017-08-01 00:00:00
abstract::A series of chemiluminescent 17beta-estradiol probes were synthesized. Relative equilibrium dissociation constants (K(D)) for the interaction of an anti-E(2) Fab fragment for the probes in solution were evaluated using a single E(2)-analog biosensor surface on a BIAcore surface plasmon resonance instrument. The result...
journal_title:Steroids
pub_type: 杂志文章
doi:10.1016/s0039-128x(00)00091-x
更新日期:2000-06-01 00:00:00